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Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana

dc.creatorDjekić, Ivana
dc.creatorBulajić, Aleksandra
dc.creatorVučurović, Ana
dc.creatorKrstić, Branka
dc.creatorJović, Jelena
dc.creatorKrnjajić, Slobodan
dc.creatorBerenji, Janoš
dc.date.accessioned2020-12-17T19:17:47Z
dc.date.available2020-12-17T19:17:47Z
dc.date.issued2008
dc.identifier.issn0351-9430
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/1779
dc.description.abstractThree-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. .en
dc.description.abstractTrogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.sr
dc.publisherNaučni institut za ratarstvo i povrtarstvo, Novi Sad
dc.rightsopenAccess
dc.sourceBilten za hmelj, sirak i lekovito bilje
dc.subjecttobaccoen
dc.subjectcucumber mosaic virusen
dc.subjectfrequencyen
dc.subjectELISAen
dc.subjectRT-PCRen
dc.subjectduvansr
dc.subjectvirus mozaika krastavcasr
dc.subjectučestalostsr
dc.subjectELISAsr
dc.subjectRT-PCRsr
dc.titleFrequency and molecular detection of cucumber mosaic virus in tobacco cropsen
dc.titleZastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvanasr
dc.typearticle
dc.rights.licenseARR
dc.citation.epage82
dc.citation.issue81
dc.citation.other40(81): 70-82
dc.citation.spage70
dc.citation.volume40
dc.identifier.rcubconv_2730
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_agrospace_1779
dc.type.versionpublishedVersion


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