Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor
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Manojlović, VericaAgouridis, Nikos
Kopsahelis, Nikos
Kanellaki, Maria
Bugarski, Branko

Nedović, Viktor

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The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of e...thanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.
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Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2008, 2, 327-334Publisher:
- 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
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Poljoprivredni fakultetTY - CONF AU - Manojlović, Verica AU - Agouridis, Nikos AU - Kopsahelis, Nikos AU - Kanellaki, Maria AU - Bugarski, Branko AU - Nedović, Viktor PY - 2008 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/1640 AB - The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of ethanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect. PB - 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists C3 - Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20 T1 - Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor EP - 334 SP - 327 VL - 2 UR - https://hdl.handle.net/21.15107/rcub_agrospace_1640 ER -
@conference{ author = "Manojlović, Verica and Agouridis, Nikos and Kopsahelis, Nikos and Kanellaki, Maria and Bugarski, Branko and Nedović, Viktor", year = "2008", abstract = "The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of ethanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.", publisher = "2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists", journal = "Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20", title = "Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor", pages = "334-327", volume = "2", url = "https://hdl.handle.net/21.15107/rcub_agrospace_1640" }
Manojlović, V., Agouridis, N., Kopsahelis, N., Kanellaki, M., Bugarski, B.,& Nedović, V.. (2008). Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists., 2, 327-334. https://hdl.handle.net/21.15107/rcub_agrospace_1640
Manojlović V, Agouridis N, Kopsahelis N, Kanellaki M, Bugarski B, Nedović V. Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20. 2008;2:327-334. https://hdl.handle.net/21.15107/rcub_agrospace_1640 .
Manojlović, Verica, Agouridis, Nikos, Kopsahelis, Nikos, Kanellaki, Maria, Bugarski, Branko, Nedović, Viktor, "Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor" in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2 (2008):327-334, https://hdl.handle.net/21.15107/rcub_agrospace_1640 .