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Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor

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Authors
Manojlović, Verica
Agouridis, Nikos
Kopsahelis, Nikos
Kanellaki, Maria
Bugarski, Branko
Nedović, Viktor
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Abstract
The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of e...thanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.

Source:
Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2008, 2, 327-334
Publisher:
  • 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists

WoS: 000269184700043

Scopus: 2-s2.0-84891400446
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3
3
Handle
https://hdl.handle.net/21.15107/rcub_agrospace_1640
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/1640
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Poljoprivredni fakultet
TY  - CONF
AU  - Manojlović, Verica
AU  - Agouridis, Nikos
AU  - Kopsahelis, Nikos
AU  - Kanellaki, Maria
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1640
AB  - The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of ethanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.
PB  - 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
C3  - Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
T1  - Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor
EP  - 334
SP  - 327
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1640
ER  - 
@conference{
author = "Manojlović, Verica and Agouridis, Nikos and Kopsahelis, Nikos and Kanellaki, Maria and Bugarski, Branko and Nedović, Viktor",
year = "2008",
abstract = "The aim of this study was to examine the novel gas-lift three phase bioreactor with external loop in beer fermentation by immobilized yeast cells on gluten pellets. Cells of Saccharomyces cerevisiae were immobilized on 40 g of gluten pellets, freeze-dried and introduced into a 1.4 L external loop gas-lift bioreactor with 1.1 L of wort. During the fermentation process, nitrogen was inserted in the bioreactor. Several batch repetitive fermentation processes were performed at different temperatures (5, 10 and 20 degrees C). The kinetics of fermentation and beer productivity were determined. Relatively high cell concentrations and presumably, good mass transfer rates in the flowing medium enabled rapid fermentation (48 h fermentation time at 20 degrees C) and high beer productivity of this system, even with such low load of immobilised biocatalyst (3.6% w/w). Further, the final beer product was analyzed on ethanol and volatile compounds important for the organolaptic profile. Contents of ethanol and flavour compounds were in the ranges characteristic for beers of acceptable to fine quality. In addition, preliminary tests on hydrodynamic characteristics of the external loop gas-lift bioreactor were done, providing important information for potential establishing and standardization of this type of fermentors for beer production in prospect.",
publisher = "2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists",
journal = "Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20",
title = "Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor",
pages = "334-327",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1640"
}
Manojlović, V., Agouridis, N., Kopsahelis, N., Kanellaki, M., Bugarski, B.,& Nedović, V.. (2008). Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists., 2, 327-334.
https://hdl.handle.net/21.15107/rcub_agrospace_1640
Manojlović V, Agouridis N, Kopsahelis N, Kanellaki M, Bugarski B, Nedović V. Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20. 2008;2:327-334.
https://hdl.handle.net/21.15107/rcub_agrospace_1640 .
Manojlović, Verica, Agouridis, Nikos, Kopsahelis, Nikos, Kanellaki, Maria, Bugarski, Branko, Nedović, Viktor, "Brewing by Immobilized Freeze Dried Cells in a Novel Gas Flow Bioreactor" in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2 (2008):327-334,
https://hdl.handle.net/21.15107/rcub_agrospace_1640 .

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