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dc.creatorYan, YM
dc.creatorŠurlan-Momirović, Gordana
dc.creatorProdanović, Slaven
dc.creatorZorić, D
dc.creatorLiu, GT
dc.date.accessioned2020-12-17T17:39:37Z
dc.date.available2020-12-17T17:39:37Z
dc.date.issued1999
dc.identifier.issn0014-2336
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/162
dc.description.abstractGliadin proteins extracted from fifteen Chinese and Yugoslav winter wheat cultivars were fractionated using a new separation technique - Capillary Zone Electrophoresis (CZE). Different CZE conditions were defined to optimize resolution and reproducibility of gliadin separations. Excellent resolution and high reproducibility of gliadin CZE patterns were obtained by using 47 cm length, 50 mu m i.d. capillaries at 15 kV and 30 degrees C in sodium berate buffer system with acetonitrile (ACN) and sodium dodecyl sulfate. By using these CZE conditions, gliadin proteins from each cultivar were easily separated into more than 35 components. This resolution is generally superior to that of one- and two-dimensional electrophoresis and RP-HPLC. Analysis of reproducibility of gliadin CZE patterns from Chinese cultivar 'Lumai 6' showed that the average relative standard deviation (RSD) for peak migration times and heights was 0.21% and 4.06%, respectively. Gliadin electrophoregrams of all cultivars studied showed clear qualitative and quantitative differences, including presence or absence of some major peak, migration times and heights of peaks. Specifically, some closely related cultivars that were not differentiable by A-PAGE, were readily differentiated by CZE. In addition, winter wheat cultivars from China and Yugoslavia showed greater differences in gliadin compositions revealed by CZE.en
dc.publisherKluwer Academic Publ, Dordrecht
dc.rightsrestrictedAccess
dc.sourceEuphytica
dc.subjectcapillary electrophoresisen
dc.subjectcultivar identificationen
dc.subjectgliadinsen
dc.subjectwheaten
dc.titleCapillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivarsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage204
dc.citation.issue3
dc.citation.other105(3): 197-204
dc.citation.rankM21
dc.citation.spage197
dc.citation.volume105
dc.identifier.doi10.1023/A:1003431602558
dc.identifier.scopus2-s2.0-0033011408
dc.identifier.wos000079330200005
dc.type.versionpublishedVersion


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