Five samples of feces from premature born nursling were taken from Institute of Neonatology (Belgrade). Three different media based on MRS was used for isolation of intestinal isolates of lactic acid bacteria. From 25 obtained cultures nine were selected for further investigation on potential probiotic ability and compared with Lactobacillus rhamnosus GG as reference strain. Preliminary identification has been performed according morphological traits (Gram staining, cells shape and colony characteristics on agar plate). Isolates suspected to lactobacilli and bifidobacteria were tested for selected physiological and biochemical characteristics. Tests for investigation of potential probiotic ability comprised antimicrobial activity against wide range of pathogens, sensitivity to simulated gastrointestinal tract conditions, and antibiotic resistance. The morphological and physiological profiles showed that all strains are Lactobacillus rhamnosus. There were some differences in their sugar... fermentation pattern as well as in their antimicrobial activities. All strain grown well in simulated gastrointestinal condition but their sensitivity was greater on bile acids and pancreatin compared with low pH (1, 2 and 3) and pepsin. Most isolates showed good probiotic potential with similar properties with referent strain Lactobacillus rhamnosus GG determined in vitro.
Keywords:
Intestinal isolates / Premature born nursling / Probiotic
Source:
Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 2008, 1, 343-349
Publisher:
2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
TY - CONF
AU - Dimitrijević, S.
AU - Nedović, Viktor
AU - Bogicević, B.
AU - Trsić, N.
AU - Petrović, Tanja
AU - Nikšić, Miomir
AU - Bugarski, Branko
AU - Lacroix, C.
PY - 2008
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/1621
AB - Five samples of feces from premature born nursling were taken from Institute of Neonatology (Belgrade). Three different media based on MRS was used for isolation of intestinal isolates of lactic acid bacteria. From 25 obtained cultures nine were selected for further investigation on potential probiotic ability and compared with Lactobacillus rhamnosus GG as reference strain. Preliminary identification has been performed according morphological traits (Gram staining, cells shape and colony characteristics on agar plate). Isolates suspected to lactobacilli and bifidobacteria were tested for selected physiological and biochemical characteristics. Tests for investigation of potential probiotic ability comprised antimicrobial activity against wide range of pathogens, sensitivity to simulated gastrointestinal tract conditions, and antibiotic resistance. The morphological and physiological profiles showed that all strains are Lactobacillus rhamnosus. There were some differences in their sugar fermentation pattern as well as in their antimicrobial activities. All strain grown well in simulated gastrointestinal condition but their sensitivity was greater on bile acids and pancreatin compared with low pH (1, 2 and 3) and pepsin. Most isolates showed good probiotic potential with similar properties with referent strain Lactobacillus rhamnosus GG determined in vitro.
PB - 2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
C3 - Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
T1 - Characterization of intestinal isolates of lactic acid bacteria with potential probiotic ability from premature born nursling
EP - 349
SP - 343
VL - 1
UR - https://hdl.handle.net/21.15107/rcub_agrospace_1621
ER -
@conference{
author = "Dimitrijević, S. and Nedović, Viktor and Bogicević, B. and Trsić, N. and Petrović, Tanja and Nikšić, Miomir and Bugarski, Branko and Lacroix, C.",
year = "2008",
abstract = "Five samples of feces from premature born nursling were taken from Institute of Neonatology (Belgrade). Three different media based on MRS was used for isolation of intestinal isolates of lactic acid bacteria. From 25 obtained cultures nine were selected for further investigation on potential probiotic ability and compared with Lactobacillus rhamnosus GG as reference strain. Preliminary identification has been performed according morphological traits (Gram staining, cells shape and colony characteristics on agar plate). Isolates suspected to lactobacilli and bifidobacteria were tested for selected physiological and biochemical characteristics. Tests for investigation of potential probiotic ability comprised antimicrobial activity against wide range of pathogens, sensitivity to simulated gastrointestinal tract conditions, and antibiotic resistance. The morphological and physiological profiles showed that all strains are Lactobacillus rhamnosus. There were some differences in their sugar fermentation pattern as well as in their antimicrobial activities. All strain grown well in simulated gastrointestinal condition but their sensitivity was greater on bile acids and pancreatin compared with low pH (1, 2 and 3) and pepsin. Most isolates showed good probiotic potential with similar properties with referent strain Lactobacillus rhamnosus GG determined in vitro.",
publisher = "2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists",
journal = "Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20",
title = "Characterization of intestinal isolates of lactic acid bacteria with potential probiotic ability from premature born nursling",
pages = "349-343",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1621"
}
Dimitrijević, S., Nedović, V., Bogicević, B., Trsić, N., Petrović, T., Nikšić, M., Bugarski, B.,& Lacroix, C.. (2008). Characterization of intestinal isolates of lactic acid bacteria with potential probiotic ability from premature born nursling. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20
2008 Joint Central European Congress of the 4th Central European Congress on Food, CEFood 2008 and 6th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists., 1, 343-349.
https://hdl.handle.net/21.15107/rcub_agrospace_1621
Dimitrijević S, Nedović V, Bogicević B, Trsić N, Petrović T, Nikšić M, Bugarski B, Lacroix C. Characterization of intestinal isolates of lactic acid bacteria with potential probiotic ability from premature born nursling. in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20. 2008;1:343-349.
https://hdl.handle.net/21.15107/rcub_agrospace_1621 .
Dimitrijević, S., Nedović, Viktor, Bogicević, B., Trsić, N., Petrović, Tanja, Nikšić, Miomir, Bugarski, Branko, Lacroix, C., "Characterization of intestinal isolates of lactic acid bacteria with potential probiotic ability from premature born nursling" in Proceedings - 2008 Joint Central European Congress: 4th Central European Congress on Food, CEFood 20, 1 (2008):343-349,
https://hdl.handle.net/21.15107/rcub_agrospace_1621 .
