Gliadin proteins extracted from fifteen Chinese and Yugoslav winter wheat cultivars were fractionated using a new separation technique - Capillary Zone Electrophoresis (CZE). Different CZE conditions were defined to optimize resolution and reproducibility of gliadin separations. Excellent resolution and high reproducibility of gliadin CZE patterns were obtained by using 47 cm length, 50 mu m i.d. capillaries at 15 kV and 30 degrees C in sodium berate buffer system with acetonitrile (ACN) and sodium dodecyl sulfate. By using these CZE conditions, gliadin proteins from each cultivar were easily separated into more than 35 components. This resolution is generally superior to that of one- and two-dimensional electrophoresis and RP-HPLC. Analysis of reproducibility of gliadin CZE patterns from Chinese cultivar 'Lumai 6' showed that the average relative standard deviation (RSD) for peak migration times and heights was 0.21% and 4.06%, respectively. Gliadin electrophoregrams of all cultivars ...studied showed clear qualitative and quantitative differences, including presence or absence of some major peak, migration times and heights of peaks. Specifically, some closely related cultivars that were not differentiable by A-PAGE, were readily differentiated by CZE. In addition, winter wheat cultivars from China and Yugoslavia showed greater differences in gliadin compositions revealed by CZE.
TY - JOUR
AU - Yan, YM
AU - Šurlan-Momirović, Gordana
AU - Prodanović, Slaven
AU - Zorić, D
AU - Liu, GT
PY - 1999
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/162
AB - Gliadin proteins extracted from fifteen Chinese and Yugoslav winter wheat cultivars were fractionated using a new separation technique - Capillary Zone Electrophoresis (CZE). Different CZE conditions were defined to optimize resolution and reproducibility of gliadin separations. Excellent resolution and high reproducibility of gliadin CZE patterns were obtained by using 47 cm length, 50 mu m i.d. capillaries at 15 kV and 30 degrees C in sodium berate buffer system with acetonitrile (ACN) and sodium dodecyl sulfate. By using these CZE conditions, gliadin proteins from each cultivar were easily separated into more than 35 components. This resolution is generally superior to that of one- and two-dimensional electrophoresis and RP-HPLC. Analysis of reproducibility of gliadin CZE patterns from Chinese cultivar 'Lumai 6' showed that the average relative standard deviation (RSD) for peak migration times and heights was 0.21% and 4.06%, respectively. Gliadin electrophoregrams of all cultivars studied showed clear qualitative and quantitative differences, including presence or absence of some major peak, migration times and heights of peaks. Specifically, some closely related cultivars that were not differentiable by A-PAGE, were readily differentiated by CZE. In addition, winter wheat cultivars from China and Yugoslavia showed greater differences in gliadin compositions revealed by CZE.
PB - Kluwer Academic Publ, Dordrecht
T2 - Euphytica
T1 - Capillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivars
EP - 204
IS - 3
SP - 197
VL - 105
DO - 10.1023/A:1003431602558
ER -
@article{
author = "Yan, YM and Šurlan-Momirović, Gordana and Prodanović, Slaven and Zorić, D and Liu, GT",
year = "1999",
abstract = "Gliadin proteins extracted from fifteen Chinese and Yugoslav winter wheat cultivars were fractionated using a new separation technique - Capillary Zone Electrophoresis (CZE). Different CZE conditions were defined to optimize resolution and reproducibility of gliadin separations. Excellent resolution and high reproducibility of gliadin CZE patterns were obtained by using 47 cm length, 50 mu m i.d. capillaries at 15 kV and 30 degrees C in sodium berate buffer system with acetonitrile (ACN) and sodium dodecyl sulfate. By using these CZE conditions, gliadin proteins from each cultivar were easily separated into more than 35 components. This resolution is generally superior to that of one- and two-dimensional electrophoresis and RP-HPLC. Analysis of reproducibility of gliadin CZE patterns from Chinese cultivar 'Lumai 6' showed that the average relative standard deviation (RSD) for peak migration times and heights was 0.21% and 4.06%, respectively. Gliadin electrophoregrams of all cultivars studied showed clear qualitative and quantitative differences, including presence or absence of some major peak, migration times and heights of peaks. Specifically, some closely related cultivars that were not differentiable by A-PAGE, were readily differentiated by CZE. In addition, winter wheat cultivars from China and Yugoslavia showed greater differences in gliadin compositions revealed by CZE.",
publisher = "Kluwer Academic Publ, Dordrecht",
journal = "Euphytica",
title = "Capillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivars",
pages = "204-197",
number = "3",
volume = "105",
doi = "10.1023/A:1003431602558"
}
Yan, Y., Šurlan-Momirović, G., Prodanović, S., Zorić, D.,& Liu, G.. (1999). Capillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivars. in Euphytica
Kluwer Academic Publ, Dordrecht., 105(3), 197-204.
https://doi.org/10.1023/A:1003431602558
Yan Y, Šurlan-Momirović G, Prodanović S, Zorić D, Liu G. Capillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivars. in Euphytica. 1999;105(3):197-204.
doi:10.1023/A:1003431602558 .
Yan, YM, Šurlan-Momirović, Gordana, Prodanović, Slaven, Zorić, D, Liu, GT, "Capillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivars" in Euphytica, 105, no. 3 (1999):197-204,
https://doi.org/10.1023/A:1003431602558 . .
