Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis

Abstract

Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.