Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.
Keywords:
A-PAGE / Genetic control / LMW-GS / Wheat
Source:
Cereal Research Communications, 1999, 27, 3, 251-257
TY - JOUR
AU - Yan, Y.
AU - Prodanović, Slaven
AU - Mladenov, N.
AU - Milovanović, M.
PY - 1999
UR - http://aspace.agrif.bg.ac.rs/handle/123456789/147
AB - Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.
T2 - Cereal Research Communications
T1 - Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis
EP - 257
IS - 3
SP - 251
VL - 27
DO - 10.1007/bf03543533
ER -
@article{
author = "Yan, Y. and Prodanović, Slaven and Mladenov, N. and Milovanović, M.",
year = "1999",
abstract = "Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.",
journal = "Cereal Research Communications",
title = "Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis",
pages = "257-251",
number = "3",
volume = "27",
doi = "10.1007/bf03543533"
}
Yan, Y., Prodanović, S., Mladenov, N.,& Milovanović, M.. (1999). Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis. in Cereal Research Communications, 27(3), 251-257.
https://doi.org/10.1007/bf03543533
Yan Y, Prodanović S, Mladenov N, Milovanović M. Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis. in Cereal Research Communications. 1999;27(3):251-257.
doi:10.1007/bf03543533 .
Yan, Y., Prodanović, Slaven, Mladenov, N., Milovanović, M., "Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis" in Cereal Research Communications, 27, no. 3 (1999):251-257,
https://doi.org/10.1007/bf03543533 . .
