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Stolbur phytoplasmas infecting chrysanthemum plants in Serbia

Stolbur fitoplazme na hrizantemi u Srbiji

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2006
1196.pdf (465.0Kb)
Authors
Duduk, Bojan
Dukić, Nataša
Bulajić, Aleksandra
Krstić, Branka
Bertaccini, Assunta
Article (Published version)
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Abstract
During a survey on ornamental crops growing in open field, chrysanthemum plants showing proliferation, virescence and stunting were observed. To verify possible phytoplasma association with described symptoms, polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses on phytoplasma 16Sr DNA were carried out. Phytoplasmas belonging to 16SrXII-A ribosomal subgroup (stolbur) were identified in all samples tested from symptomatic plants. This first report of 16SrXII-A stolbur phytoplasmas in chrysanthemum with flower proliferation and stunting symptoms allow to distinguish this from already known chrysanthemum yellows associated with phytoplasmas belonging to aster yellows 16Srl-B ribosomal group, "Candidatus Phytoplasma asteris".
Tokom pregleda otvorenog polja sa ukrasnim biljkama na lokalitetu Vinča, Beograd, primećene su biljke hrizanteme sa simptomima fitoplazmoza. Primećeni simptomi su obuhvatali proliferaciju cvetova, virescenciju i kržljavost. Radi provere moguće zaraze fitoplazmama, simptomatične biljke hrizanteme analizirane su molekularnim tehnikama, umetnutom lančanom reakcijom polimeraze (nested PCR) praćenom analizom polimorfizma dužine restrikcionih fragmenata (RFLP) na 16Sr DNA. U svim testiranim uzorcima uzetim sa simtomatičnih biljaka hrizanteme identifikovane su fitoplazme iz 16SrXII-A ribozomalne podgrupe. Prvi nalaz 16SrXII-A stolbur fitoplazmi u biljkama hrizanteme sa simptomima proliferacije cvetova, virescencije i kržljavosti razlikuje ovu fitoplazmozu od već poznate "chrysanthemum yellows" kojaje povezana sa fitoplazmama koje pripadaju aster yellows 16Srl-B ribozomalnoj podgrupi, "Candidatus Phytoplasma asteris". .
Keywords:
Chrysanthemum / Chrysanthemum indicum hybridum / Stolbur phyto plasma / hrizantema / Chrysanthemum indicumhybridum / stolbur fitoplazma
Source:
Pesticidi i fitomedicina, 2006, 21, 2, 107-112
Publisher:
  • Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd

ISSN: 1820-3949

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_agrospace_1199
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/1199
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Poljoprivredni fakultet
TY  - JOUR
AU  - Duduk, Bojan
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
AU  - Bertaccini, Assunta
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1199
AB  - During a survey on ornamental crops growing in open field, chrysanthemum plants showing proliferation, virescence and stunting were observed. To verify possible phytoplasma association with described symptoms, polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses on phytoplasma 16Sr DNA were carried out. Phytoplasmas belonging to 16SrXII-A ribosomal subgroup (stolbur) were identified in all samples tested from symptomatic plants. This first report of 16SrXII-A stolbur phytoplasmas in chrysanthemum with flower proliferation and stunting symptoms allow to distinguish this from already known chrysanthemum yellows associated with phytoplasmas belonging to aster yellows 16Srl-B ribosomal group, "Candidatus Phytoplasma asteris".
AB  - Tokom pregleda otvorenog polja sa ukrasnim biljkama na lokalitetu Vinča, Beograd, primećene su biljke hrizanteme sa simptomima fitoplazmoza. Primećeni simptomi su obuhvatali proliferaciju cvetova, virescenciju i kržljavost. Radi provere moguće zaraze fitoplazmama, simptomatične biljke hrizanteme analizirane su molekularnim tehnikama, umetnutom lančanom reakcijom polimeraze (nested PCR) praćenom analizom polimorfizma dužine restrikcionih fragmenata (RFLP) na 16Sr DNA. U svim testiranim uzorcima uzetim sa simtomatičnih biljaka hrizanteme identifikovane su fitoplazme iz 16SrXII-A ribozomalne podgrupe. Prvi nalaz 16SrXII-A stolbur fitoplazmi u biljkama hrizanteme sa simptomima proliferacije cvetova, virescencije i kržljavosti razlikuje ovu fitoplazmozu od već poznate "chrysanthemum yellows" kojaje povezana sa fitoplazmama koje pripadaju aster yellows 16Srl-B ribozomalnoj podgrupi, "Candidatus Phytoplasma asteris". .
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Stolbur phytoplasmas infecting chrysanthemum plants in Serbia
T1  - Stolbur fitoplazme na hrizantemi u Srbiji
EP  - 112
IS  - 2
SP  - 107
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1199
ER  - 
@article{
author = "Duduk, Bojan and Dukić, Nataša and Bulajić, Aleksandra and Krstić, Branka and Bertaccini, Assunta",
year = "2006",
abstract = "During a survey on ornamental crops growing in open field, chrysanthemum plants showing proliferation, virescence and stunting were observed. To verify possible phytoplasma association with described symptoms, polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses on phytoplasma 16Sr DNA were carried out. Phytoplasmas belonging to 16SrXII-A ribosomal subgroup (stolbur) were identified in all samples tested from symptomatic plants. This first report of 16SrXII-A stolbur phytoplasmas in chrysanthemum with flower proliferation and stunting symptoms allow to distinguish this from already known chrysanthemum yellows associated with phytoplasmas belonging to aster yellows 16Srl-B ribosomal group, "Candidatus Phytoplasma asteris"., Tokom pregleda otvorenog polja sa ukrasnim biljkama na lokalitetu Vinča, Beograd, primećene su biljke hrizanteme sa simptomima fitoplazmoza. Primećeni simptomi su obuhvatali proliferaciju cvetova, virescenciju i kržljavost. Radi provere moguće zaraze fitoplazmama, simptomatične biljke hrizanteme analizirane su molekularnim tehnikama, umetnutom lančanom reakcijom polimeraze (nested PCR) praćenom analizom polimorfizma dužine restrikcionih fragmenata (RFLP) na 16Sr DNA. U svim testiranim uzorcima uzetim sa simtomatičnih biljaka hrizanteme identifikovane su fitoplazme iz 16SrXII-A ribozomalne podgrupe. Prvi nalaz 16SrXII-A stolbur fitoplazmi u biljkama hrizanteme sa simptomima proliferacije cvetova, virescencije i kržljavosti razlikuje ovu fitoplazmozu od već poznate "chrysanthemum yellows" kojaje povezana sa fitoplazmama koje pripadaju aster yellows 16Srl-B ribozomalnoj podgrupi, "Candidatus Phytoplasma asteris". .",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Stolbur phytoplasmas infecting chrysanthemum plants in Serbia, Stolbur fitoplazme na hrizantemi u Srbiji",
pages = "112-107",
number = "2",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1199"
}
Duduk, B., Dukić, N., Bulajić, A., Krstić, B.,& Bertaccini, A.. (2006). Stolbur phytoplasmas infecting chrysanthemum plants in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 21(2), 107-112.
https://hdl.handle.net/21.15107/rcub_agrospace_1199
Duduk B, Dukić N, Bulajić A, Krstić B, Bertaccini A. Stolbur phytoplasmas infecting chrysanthemum plants in Serbia. in Pesticidi i fitomedicina. 2006;21(2):107-112.
https://hdl.handle.net/21.15107/rcub_agrospace_1199 .
Duduk, Bojan, Dukić, Nataša, Bulajić, Aleksandra, Krstić, Branka, Bertaccini, Assunta, "Stolbur phytoplasmas infecting chrysanthemum plants in Serbia" in Pesticidi i fitomedicina, 21, no. 2 (2006):107-112,
https://hdl.handle.net/21.15107/rcub_agrospace_1199 .

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