TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Flath, Kerstin
AU  - Silvar, Cristina
AU  - Branković, Gordana
AU  - Kopahnke, Doris
AU  - Knežević, Desimir
AU  - Schliephake, Edgar
AU  - Ordon, Frank
AU  - Perović, Dragan
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4151
AB  - Unlocking resistance genes in genbank collections are of prime importance for securing sustainable crop production. In this regard, the Serbian GenBank barley collection, comprising 93 local landraces and 36 commercial cultivars and elite barley breeding lines, was screened for novel resistances to powdery mildew (Blumeria graminis f. sp. hordei) using a set of 28 isolates with a wide spectrum of virulences/avirulences. No line was resistant to all the isolates, but one and three accessions showed resistance to 27 and 26 isolates, respectively. Twenty landraces (21.51 %) and ten cultivars (27.78 %) exhibited resistance to 50 % of the isolates. Infection type 2 was most frequent among resistant accessions. Nine B. graminis isolates were sufficient for gene postulation in 73 barley lines. In total, thirty-five different resistance spectra were recorded and the following known resistance genes were postulated namely, Mlra, Mlh, Mla12, Mla7(Mlu), Mlg, MlLa, Mla6, Mla7, Mlt, Mla22, Mlat, Mla1, Mlk. The majority of resistance profiles was constituted by only one line. Unidentified genes alone or in combination were proposed for twenty landraces and six cultivars. This report demonstrated that the barley collection held at the Serbian GenBank could be exploited as a new source for powdery mildew resistance.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance
EP  - 287
IS  - 2
SP  - 275
VL  - 63
DO  - 10.1007/s10722-015-0246-2
ER  - 

@article{
author = "Šurlan-Momirović, Gordana and Flath, Kerstin and Silvar, Cristina and Branković, Gordana and Kopahnke, Doris and Knežević, Desimir and Schliephake, Edgar and Ordon, Frank and Perović, Dragan",
year = "2016",
abstract = "Unlocking resistance genes in genbank collections are of prime importance for securing sustainable crop production. In this regard, the Serbian GenBank barley collection, comprising 93 local landraces and 36 commercial cultivars and elite barley breeding lines, was screened for novel resistances to powdery mildew (Blumeria graminis f. sp. hordei) using a set of 28 isolates with a wide spectrum of virulences/avirulences. No line was resistant to all the isolates, but one and three accessions showed resistance to 27 and 26 isolates, respectively. Twenty landraces (21.51 %) and ten cultivars (27.78 %) exhibited resistance to 50 % of the isolates. Infection type 2 was most frequent among resistant accessions. Nine B. graminis isolates were sufficient for gene postulation in 73 barley lines. In total, thirty-five different resistance spectra were recorded and the following known resistance genes were postulated namely, Mlra, Mlh, Mla12, Mla7(Mlu), Mlg, MlLa, Mla6, Mla7, Mlt, Mla22, Mlat, Mla1, Mlk. The majority of resistance profiles was constituted by only one line. Unidentified genes alone or in combination were proposed for twenty landraces and six cultivars. This report demonstrated that the barley collection held at the Serbian GenBank could be exploited as a new source for powdery mildew resistance.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance",
pages = "287-275",
number = "2",
volume = "63",
doi = "10.1007/s10722-015-0246-2"
}

Šurlan-Momirović, G., Flath, K., Silvar, C., Branković, G., Kopahnke, D., Knežević, D., Schliephake, E., Ordon, F.,& Perović, D.. (2016). Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance. in Genetic Resources and Crop Evolution
Springer, Dordrecht., 63(2), 275-287.
https://doi.org/10.1007/s10722-015-0246-2

Šurlan-Momirović G, Flath K, Silvar C, Branković G, Kopahnke D, Knežević D, Schliephake E, Ordon F, Perović D. Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance. in Genetic Resources and Crop Evolution. 2016;63(2):275-287.
doi:10.1007/s10722-015-0246-2 .

Šurlan-Momirović, Gordana, Flath, Kerstin, Silvar, Cristina, Branković, Gordana, Kopahnke, Doris, Knežević, Desimir, Schliephake, Edgar, Ordon, Frank, Perović, Dragan, "Exploring the Serbian GenBank barley (Hordeum vulgare L. subsp vulgare) collection for powdery mildew resistance" in Genetic Resources and Crop Evolution, 63, no. 2 (2016):275-287,
https://doi.org/10.1007/s10722-015-0246-2 . .

TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Krämer, Ilona
AU  - Bratković, Kamenko
AU  - Zorić, Miroslav
AU  - Momirović, Una
AU  - Branković, Gordana
AU  - Ćalić, Irena
AU  - Kandić, Vesna
AU  - Pržulj, Novo
AU  - Ordon, Frank
AU  - Perović, Dragan
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3123
AB  - Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes.
AB  - Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting
T1  - Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima
EP  - 180
IS  - 1
SP  - 167
VL  - 45
DO  - 10.2298/GENSR1301167S
ER  - 

@article{
author = "Šurlan-Momirović, Gordana and Krämer, Ilona and Bratković, Kamenko and Zorić, Miroslav and Momirović, Una and Branković, Gordana and Ćalić, Irena and Kandić, Vesna and Pržulj, Novo and Ordon, Frank and Perović, Dragan",
year = "2013",
abstract = "Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes., Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting, Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima",
pages = "180-167",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301167S"
}

Šurlan-Momirović, G., Krämer, I., Bratković, K., Zorić, M., Momirović, U., Branković, G., Ćalić, I., Kandić, V., Pržulj, N., Ordon, F.,& Perović, D.. (2013). Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika
Društvo genetičara Srbije, Beograd., 45(1), 167-180.
https://doi.org/10.2298/GENSR1301167S

Šurlan-Momirović G, Krämer I, Bratković K, Zorić M, Momirović U, Branković G, Ćalić I, Kandić V, Pržulj N, Ordon F, Perović D. Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika. 2013;45(1):167-180.
doi:10.2298/GENSR1301167S .

Šurlan-Momirović, Gordana, Krämer, Ilona, Bratković, Kamenko, Zorić, Miroslav, Momirović, Una, Branković, Gordana, Ćalić, Irena, Kandić, Vesna, Pržulj, Novo, Ordon, Frank, Perović, Dragan, "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting" in Genetika, 45, no. 1 (2013):167-180,
https://doi.org/10.2298/GENSR1301167S . .