TY  - JOUR
AU  - Dimitrellou, Dimitra
AU  - Kandylis, Panagiotis
AU  - Lević, Steva
AU  - Petrović, Tanja
AU  - Ivanović, Sanjin
AU  - Nedović, Viktor
AU  - Kourkoutas, Yiannis
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4989
AB  - The aim of the present study was to investigate encapsulation of probiotic Lactobacillus casei ATCC 393 on alginates using the extrusion technique and to evaluate cell survival under simulated gastrointestinal conditions and during fermented milk production and storage at 4 degrees C for up to four weeks. Encapsulation of probiotic bacteria led to significantly higher viabilities compared to free cells under simulated gastrointestinal conditions, as well as after storage for 28 days in fermented milk (7.13 log cfu g(-1)). In addition, the effect of encapsulated L. casei ATCC 393 cells on aroma volatile compounds formation during milk fermentation was studied using the SPME GC/MS technique. The fermented milks presented improved aroma, containing characteristic compounds produced usually by L. casei in fermented dairy products. The results showed that the alginate matrix has a great potential as encapsulation material for probiotics and application of the encapsulated cells in fermented dairy products may result in effective maintenance of cell survival during refrigerated storage and improvement of sensory characteristics. From an economical point of view and under certain conditions, the application of encapsulated probiotic cells is a sustainable process for the production of fermented milk.
PB  - Elsevier, Amsterdam
T2  - LWT-Food Science and Technology
T1  - Encapsulation of Lactobacillus casei ATCC 393 in alginate capsules for probiotic fermented milk production
VL  - 116
DO  - 10.1016/j.lwt.2019.108501
ER  - 

@article{
author = "Dimitrellou, Dimitra and Kandylis, Panagiotis and Lević, Steva and Petrović, Tanja and Ivanović, Sanjin and Nedović, Viktor and Kourkoutas, Yiannis",
year = "2019",
abstract = "The aim of the present study was to investigate encapsulation of probiotic Lactobacillus casei ATCC 393 on alginates using the extrusion technique and to evaluate cell survival under simulated gastrointestinal conditions and during fermented milk production and storage at 4 degrees C for up to four weeks. Encapsulation of probiotic bacteria led to significantly higher viabilities compared to free cells under simulated gastrointestinal conditions, as well as after storage for 28 days in fermented milk (7.13 log cfu g(-1)). In addition, the effect of encapsulated L. casei ATCC 393 cells on aroma volatile compounds formation during milk fermentation was studied using the SPME GC/MS technique. The fermented milks presented improved aroma, containing characteristic compounds produced usually by L. casei in fermented dairy products. The results showed that the alginate matrix has a great potential as encapsulation material for probiotics and application of the encapsulated cells in fermented dairy products may result in effective maintenance of cell survival during refrigerated storage and improvement of sensory characteristics. From an economical point of view and under certain conditions, the application of encapsulated probiotic cells is a sustainable process for the production of fermented milk.",
publisher = "Elsevier, Amsterdam",
journal = "LWT-Food Science and Technology",
title = "Encapsulation of Lactobacillus casei ATCC 393 in alginate capsules for probiotic fermented milk production",
volume = "116",
doi = "10.1016/j.lwt.2019.108501"
}

Dimitrellou, D., Kandylis, P., Lević, S., Petrović, T., Ivanović, S., Nedović, V.,& Kourkoutas, Y.. (2019). Encapsulation of Lactobacillus casei ATCC 393 in alginate capsules for probiotic fermented milk production. in LWT-Food Science and Technology
Elsevier, Amsterdam., 116.
https://doi.org/10.1016/j.lwt.2019.108501

Dimitrellou D, Kandylis P, Lević S, Petrović T, Ivanović S, Nedović V, Kourkoutas Y. Encapsulation of Lactobacillus casei ATCC 393 in alginate capsules for probiotic fermented milk production. in LWT-Food Science and Technology. 2019;116.
doi:10.1016/j.lwt.2019.108501 .

Dimitrellou, Dimitra, Kandylis, Panagiotis, Lević, Steva, Petrović, Tanja, Ivanović, Sanjin, Nedović, Viktor, Kourkoutas, Yiannis, "Encapsulation of Lactobacillus casei ATCC 393 in alginate capsules for probiotic fermented milk production" in LWT-Food Science and Technology, 116 (2019),
https://doi.org/10.1016/j.lwt.2019.108501 . .

TY  - JOUR
AU  - Dimitrellou, Dimitra
AU  - Kandylis, Panagiotis
AU  - Petrović, Tanja
AU  - Dimitrijević-Branković, Suzana
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Kourkoutas, Yiannis
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4156
AB  - In the present study, the survival of spray-dried microencapsulated Lactobacillus casei ATCC 393 cells was evaluated under simulated gastrointestinal conditions, as well as during production and storage of fermented milk. Under simulated gastric juice and bile salts solutions, microencapsulation provided significantly improved protection compared to free cells. Furthermore, it retained a significantly higher number of viable L. casei compared to free cell system during fermented milk production. No significant differences in the physicochemical and sensorial characteristics of produced fermented milk with spray dried L. casei cells were observed compared to the control and commercial samples, which is an important feature for consumers' acceptability. Importantly, after all in vitro tests, the number of microencapsulated L. casei cells remained over 6 log cfu g(-1), in contrast to free cells, which is the minimum required level for conferring a probiotic effect.
PB  - Elsevier Science Bv, Amsterdam
T2  - LWT-Food Science and Technology
T1  - Survival of spray dried microencapsulated Lactobacillus casei ATCC 393 in simulated gastrointestinal conditions and fermented milk
EP  - 174
SP  - 169
VL  - 71
DO  - 10.1016/j.lwt.2016.03.007
ER  - 

@article{
author = "Dimitrellou, Dimitra and Kandylis, Panagiotis and Petrović, Tanja and Dimitrijević-Branković, Suzana and Lević, Steva and Nedović, Viktor and Kourkoutas, Yiannis",
year = "2016",
abstract = "In the present study, the survival of spray-dried microencapsulated Lactobacillus casei ATCC 393 cells was evaluated under simulated gastrointestinal conditions, as well as during production and storage of fermented milk. Under simulated gastric juice and bile salts solutions, microencapsulation provided significantly improved protection compared to free cells. Furthermore, it retained a significantly higher number of viable L. casei compared to free cell system during fermented milk production. No significant differences in the physicochemical and sensorial characteristics of produced fermented milk with spray dried L. casei cells were observed compared to the control and commercial samples, which is an important feature for consumers' acceptability. Importantly, after all in vitro tests, the number of microencapsulated L. casei cells remained over 6 log cfu g(-1), in contrast to free cells, which is the minimum required level for conferring a probiotic effect.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "LWT-Food Science and Technology",
title = "Survival of spray dried microencapsulated Lactobacillus casei ATCC 393 in simulated gastrointestinal conditions and fermented milk",
pages = "174-169",
volume = "71",
doi = "10.1016/j.lwt.2016.03.007"
}

Dimitrellou, D., Kandylis, P., Petrović, T., Dimitrijević-Branković, S., Lević, S., Nedović, V.,& Kourkoutas, Y.. (2016). Survival of spray dried microencapsulated Lactobacillus casei ATCC 393 in simulated gastrointestinal conditions and fermented milk. in LWT-Food Science and Technology
Elsevier Science Bv, Amsterdam., 71, 169-174.
https://doi.org/10.1016/j.lwt.2016.03.007

Dimitrellou D, Kandylis P, Petrović T, Dimitrijević-Branković S, Lević S, Nedović V, Kourkoutas Y. Survival of spray dried microencapsulated Lactobacillus casei ATCC 393 in simulated gastrointestinal conditions and fermented milk. in LWT-Food Science and Technology. 2016;71:169-174.
doi:10.1016/j.lwt.2016.03.007 .

Dimitrellou, Dimitra, Kandylis, Panagiotis, Petrović, Tanja, Dimitrijević-Branković, Suzana, Lević, Steva, Nedović, Viktor, Kourkoutas, Yiannis, "Survival of spray dried microencapsulated Lactobacillus casei ATCC 393 in simulated gastrointestinal conditions and fermented milk" in LWT-Food Science and Technology, 71 (2016):169-174,
https://doi.org/10.1016/j.lwt.2016.03.007 . .