TY  - JOUR
AU  - Machado-Moreira, Bernardino
AU  - Monteiro, Silvia
AU  - Santos, Ricardo
AU  - Martinez-Murcia, Antonio
AU  - Rajković, Andreja
AU  - Šmigić, Nada
AU  - Richards, Karl G.
AU  - Abram, Florence
AU  - Burgess, Catherine M.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5356
AB  - There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.
PB  - Elsevier, Amsterdam
T2  - Journal of Microbiological Methods
T1  - Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR
VL  - 168
DO  - 10.1016/j.mimet.2019.105786
ER  - 

@article{
author = "Machado-Moreira, Bernardino and Monteiro, Silvia and Santos, Ricardo and Martinez-Murcia, Antonio and Rajković, Andreja and Šmigić, Nada and Richards, Karl G. and Abram, Florence and Burgess, Catherine M.",
year = "2020",
abstract = "There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Microbiological Methods",
title = "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR",
volume = "168",
doi = "10.1016/j.mimet.2019.105786"
}

Machado-Moreira, B., Monteiro, S., Santos, R., Martinez-Murcia, A., Rajković, A., Šmigić, N., Richards, K. G., Abram, F.,& Burgess, C. M.. (2020). Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. in Journal of Microbiological Methods
Elsevier, Amsterdam., 168.
https://doi.org/10.1016/j.mimet.2019.105786

Machado-Moreira B, Monteiro S, Santos R, Martinez-Murcia A, Rajković A, Šmigić N, Richards KG, Abram F, Burgess CM. Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. in Journal of Microbiological Methods. 2020;168.
doi:10.1016/j.mimet.2019.105786 .

Machado-Moreira, Bernardino, Monteiro, Silvia, Santos, Ricardo, Martinez-Murcia, Antonio, Rajković, Andreja, Šmigić, Nada, Richards, Karl G., Abram, Florence, Burgess, Catherine M., "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR" in Journal of Microbiological Methods, 168 (2020),
https://doi.org/10.1016/j.mimet.2019.105786 . .

TY  - JOUR
AU  - Gunnarsdottir, Maria J.
AU  - Gardarsson, Sigurdur M.
AU  - Figueras, Maria J.
AU  - Puigdomenech, Claudia
AU  - Juarez, Ruben
AU  - Saucedo, Gemma
AU  - Jose Arnedo, M.
AU  - Santos, Ricardo
AU  - Monteiro, Silvia
AU  - Avery, Lisa
AU  - Pagaling, Eulyn
AU  - Allan, Richard
AU  - Abel, Claire
AU  - Eglitis, Janis
AU  - Hambsch, Beate
AU  - Huegler, Michael
AU  - Rajković, Andreja
AU  - Šmigić, Nada
AU  - Udovički, Božidar
AU  - Albrechtsen, Hans-Jorgen
AU  - Lopez-Aviles, Alma
AU  - Hunter, Paul
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5360
AB  - Drinking water quality has been regulated in most European countries for nearly two decades by the drinking water directive 98/83/EC. The directive is now under revision with the goal of meeting stricter demands for safe water for all citizens, as safe water has been recognized as a human right by the United Nations. An important change to the directive is the implementation of a risk-based approach in all regulated water supplies. The European Union Framework Seventh Programme Aquavalens project has developed several new detection technologies for pathogens and indicators and tested them in water supplies in seven European countries. One of the tasks of the project was to evaluate the impact of these new techniques on water safety and on water safety management. Data were collected on risk factors to water safety for five large supplies in Denmark, Germany, Spain and the UK, and for fifteen small water supplies in Scotland, Portugal and Serbia, via a questionnaire aiming to ascertain risk factors and the stage of implementation of Water Safety Plans, and via site-specific surveys known as Sanitary Site Inspection. Samples we re collected from the water supplies from all stages of water production to delivery. Pathogens were detected in around 23% of the 470 samples tested. Fecal contamination was high in raw water and even in treated water at the small supplies. Old infrastructure was considered a challenge at all the water supplies. The results showed that some of the technique, if implemented as part of the water safety management, can detect rapidly the most common waterborne pathogens and fecal pollution indicators and therefore have a great early warning potential; can improve water safety for the consumer; can validate whether mitigation methods are working as intended; and can confirm the quality of the water at source and at the tap.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Water safety plan enhancements with improved drinking water quality detection techniques
VL  - 698
DO  - 10.1016/j.scitotenv.2019.134185
ER  - 

@article{
author = "Gunnarsdottir, Maria J. and Gardarsson, Sigurdur M. and Figueras, Maria J. and Puigdomenech, Claudia and Juarez, Ruben and Saucedo, Gemma and Jose Arnedo, M. and Santos, Ricardo and Monteiro, Silvia and Avery, Lisa and Pagaling, Eulyn and Allan, Richard and Abel, Claire and Eglitis, Janis and Hambsch, Beate and Huegler, Michael and Rajković, Andreja and Šmigić, Nada and Udovički, Božidar and Albrechtsen, Hans-Jorgen and Lopez-Aviles, Alma and Hunter, Paul",
year = "2020",
abstract = "Drinking water quality has been regulated in most European countries for nearly two decades by the drinking water directive 98/83/EC. The directive is now under revision with the goal of meeting stricter demands for safe water for all citizens, as safe water has been recognized as a human right by the United Nations. An important change to the directive is the implementation of a risk-based approach in all regulated water supplies. The European Union Framework Seventh Programme Aquavalens project has developed several new detection technologies for pathogens and indicators and tested them in water supplies in seven European countries. One of the tasks of the project was to evaluate the impact of these new techniques on water safety and on water safety management. Data were collected on risk factors to water safety for five large supplies in Denmark, Germany, Spain and the UK, and for fifteen small water supplies in Scotland, Portugal and Serbia, via a questionnaire aiming to ascertain risk factors and the stage of implementation of Water Safety Plans, and via site-specific surveys known as Sanitary Site Inspection. Samples we re collected from the water supplies from all stages of water production to delivery. Pathogens were detected in around 23% of the 470 samples tested. Fecal contamination was high in raw water and even in treated water at the small supplies. Old infrastructure was considered a challenge at all the water supplies. The results showed that some of the technique, if implemented as part of the water safety management, can detect rapidly the most common waterborne pathogens and fecal pollution indicators and therefore have a great early warning potential; can improve water safety for the consumer; can validate whether mitigation methods are working as intended; and can confirm the quality of the water at source and at the tap.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Water safety plan enhancements with improved drinking water quality detection techniques",
volume = "698",
doi = "10.1016/j.scitotenv.2019.134185"
}

Gunnarsdottir, M. J., Gardarsson, S. M., Figueras, M. J., Puigdomenech, C., Juarez, R., Saucedo, G., Jose Arnedo, M., Santos, R., Monteiro, S., Avery, L., Pagaling, E., Allan, R., Abel, C., Eglitis, J., Hambsch, B., Huegler, M., Rajković, A., Šmigić, N., Udovički, B., Albrechtsen, H., Lopez-Aviles, A.,& Hunter, P.. (2020). Water safety plan enhancements with improved drinking water quality detection techniques. in Science of the Total Environment
Elsevier, Amsterdam., 698.
https://doi.org/10.1016/j.scitotenv.2019.134185

Gunnarsdottir MJ, Gardarsson SM, Figueras MJ, Puigdomenech C, Juarez R, Saucedo G, Jose Arnedo M, Santos R, Monteiro S, Avery L, Pagaling E, Allan R, Abel C, Eglitis J, Hambsch B, Huegler M, Rajković A, Šmigić N, Udovički B, Albrechtsen H, Lopez-Aviles A, Hunter P. Water safety plan enhancements with improved drinking water quality detection techniques. in Science of the Total Environment. 2020;698.
doi:10.1016/j.scitotenv.2019.134185 .

Gunnarsdottir, Maria J., Gardarsson, Sigurdur M., Figueras, Maria J., Puigdomenech, Claudia, Juarez, Ruben, Saucedo, Gemma, Jose Arnedo, M., Santos, Ricardo, Monteiro, Silvia, Avery, Lisa, Pagaling, Eulyn, Allan, Richard, Abel, Claire, Eglitis, Janis, Hambsch, Beate, Huegler, Michael, Rajković, Andreja, Šmigić, Nada, Udovički, Božidar, Albrechtsen, Hans-Jorgen, Lopez-Aviles, Alma, Hunter, Paul, "Water safety plan enhancements with improved drinking water quality detection techniques" in Science of the Total Environment, 698 (2020),
https://doi.org/10.1016/j.scitotenv.2019.134185 . .