TY  - JOUR
AU  - Zhu, Dong
AU  - Liu, Yanlin
AU  - Jin, Man
AU  - Chen, Guanxing
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5055
AB  - Expansins are a group of plant cell wall loosening proteins that play important roles in plant growth and development. In this study, we performed the first study on the molecular characterization, transcriptional expression and functional properties of two wheat expansin genes TaEXPA2 and TaEXPB1. The results indicated that TaEXPA2 and TaEXPB1 genes had typical structural features of plant expansin gene family. As a member of alpha-expansins, TaEXPA2 is closely related to rice OsEXPA17 while the beta-expansin member TaEXPB1 has closely phylogenetic relationships with rice OsEXPAB4. The genetic transformation to Arabidopsis showed that both TaEXPA2 and TaEXPB1 were located in cell wall and highly expressed in roots, leaves and seeds. Overexpression of TaEXPA2 and TaEXPB1 genes showed similar functions, causing rapid root elongation, early bolting, and increases in leaves number, rosette diameter and stems length. These results demonstrated that wheat expansin genes TaEXPA1 and TaEXPB2 can enhance plant growth and development.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Expression and function analysis of wheat expasin genes expa2 and expb1
EP  - 274
IS  - 1
SP  - 261
VL  - 51
DO  - 10.2298/GENSR1901261Z
ER  - 

@article{
author = "Zhu, Dong and Liu, Yanlin and Jin, Man and Chen, Guanxing and Prodanović, Slaven and Yan, Yueming",
year = "2019",
abstract = "Expansins are a group of plant cell wall loosening proteins that play important roles in plant growth and development. In this study, we performed the first study on the molecular characterization, transcriptional expression and functional properties of two wheat expansin genes TaEXPA2 and TaEXPB1. The results indicated that TaEXPA2 and TaEXPB1 genes had typical structural features of plant expansin gene family. As a member of alpha-expansins, TaEXPA2 is closely related to rice OsEXPA17 while the beta-expansin member TaEXPB1 has closely phylogenetic relationships with rice OsEXPAB4. The genetic transformation to Arabidopsis showed that both TaEXPA2 and TaEXPB1 were located in cell wall and highly expressed in roots, leaves and seeds. Overexpression of TaEXPA2 and TaEXPB1 genes showed similar functions, causing rapid root elongation, early bolting, and increases in leaves number, rosette diameter and stems length. These results demonstrated that wheat expansin genes TaEXPA1 and TaEXPB2 can enhance plant growth and development.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Expression and function analysis of wheat expasin genes expa2 and expb1",
pages = "274-261",
number = "1",
volume = "51",
doi = "10.2298/GENSR1901261Z"
}

Zhu, D., Liu, Y., Jin, M., Chen, G., Prodanović, S.,& Yan, Y.. (2019). Expression and function analysis of wheat expasin genes expa2 and expb1. in Genetika
Društvo genetičara Srbije, Beograd., 51(1), 261-274.
https://doi.org/10.2298/GENSR1901261Z

Zhu D, Liu Y, Jin M, Chen G, Prodanović S, Yan Y. Expression and function analysis of wheat expasin genes expa2 and expb1. in Genetika. 2019;51(1):261-274.
doi:10.2298/GENSR1901261Z .

Zhu, Dong, Liu, Yanlin, Jin, Man, Chen, Guanxing, Prodanović, Slaven, Yan, Yueming, "Expression and function analysis of wheat expasin genes expa2 and expb1" in Genetika, 51, no. 1 (2019):261-274,
https://doi.org/10.2298/GENSR1901261Z . .

TY  - JOUR
AU  - Wang, Wanqing
AU  - Wang, Ke
AU  - Chen, Xi
AU  - Prodanović, Slaven
AU  - Li, Xiaohui
AU  - Ye, Xingguo
AU  - Yan, Yueming
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4648
AB  - Gliadins, as the major components of wheat storage proteins, determine the extensibility properties of dough and have important effects on flour processing quality. Wheat related species carries potential storage protein gene resources for quality improvement. In this study, we isolated and characterized the first complete omega-gliadin gene Omega-AS from Aegilops speltoides L. (2n = 2x = 14, SS) by allelic-specific PCR and investigated its phylogenetic relationships among Triticum and Aegilops species. Molecular structure showed that Omega-AS gene consisted of 1122 bp encoding 373 amino acid residues with deduced molecular mass 41379.21 Da. Omega-AS gene was exceptionally rich in prolines and glutamines with fewer methionine and no cysteine. Sequence characterization and epitope analysis showed that three epitopes QQPIPVQPQQ, TQPQQPTPIQ and IQPQQPFPQQ were absent in Omega-AS gene encoded protein, indicating its potential value for wheat quality improvement with less toxic, or no toxic peptides. Phylogenetic analysis revealed that Omega-AS was closely related to gliadin genes of wheat and related species and its divergence from bread wheat was more recently (less than 1.243 MYA). Heterologous expression showed that Omega-AS gene could successfully express with a high level in E. coli under the control of T-7 promoter. The transcription expression pattern of Omega AS gene during grain development detected by qRT-PCR revealed that the highest expression level occurred at 17 days post anthesis.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.
EP  - 517
IS  - 2
SP  - 503
VL  - 50
DO  - 10.2298/GENSR1802503W
ER  - 

@article{
author = "Wang, Wanqing and Wang, Ke and Chen, Xi and Prodanović, Slaven and Li, Xiaohui and Ye, Xingguo and Yan, Yueming",
year = "2018",
abstract = "Gliadins, as the major components of wheat storage proteins, determine the extensibility properties of dough and have important effects on flour processing quality. Wheat related species carries potential storage protein gene resources for quality improvement. In this study, we isolated and characterized the first complete omega-gliadin gene Omega-AS from Aegilops speltoides L. (2n = 2x = 14, SS) by allelic-specific PCR and investigated its phylogenetic relationships among Triticum and Aegilops species. Molecular structure showed that Omega-AS gene consisted of 1122 bp encoding 373 amino acid residues with deduced molecular mass 41379.21 Da. Omega-AS gene was exceptionally rich in prolines and glutamines with fewer methionine and no cysteine. Sequence characterization and epitope analysis showed that three epitopes QQPIPVQPQQ, TQPQQPTPIQ and IQPQQPFPQQ were absent in Omega-AS gene encoded protein, indicating its potential value for wheat quality improvement with less toxic, or no toxic peptides. Phylogenetic analysis revealed that Omega-AS was closely related to gliadin genes of wheat and related species and its divergence from bread wheat was more recently (less than 1.243 MYA). Heterologous expression showed that Omega-AS gene could successfully express with a high level in E. coli under the control of T-7 promoter. The transcription expression pattern of Omega AS gene during grain development detected by qRT-PCR revealed that the highest expression level occurred at 17 days post anthesis.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.",
pages = "517-503",
number = "2",
volume = "50",
doi = "10.2298/GENSR1802503W"
}

Wang, W., Wang, K., Chen, X., Prodanović, S., Li, X., Ye, X.,& Yan, Y.. (2018). Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.. in Genetika
Društvo genetičara Srbije, Beograd., 50(2), 503-517.
https://doi.org/10.2298/GENSR1802503W

Wang W, Wang K, Chen X, Prodanović S, Li X, Ye X, Yan Y. Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.. in Genetika. 2018;50(2):503-517.
doi:10.2298/GENSR1802503W .

Wang, Wanqing, Wang, Ke, Chen, Xi, Prodanović, Slaven, Li, Xiaohui, Ye, Xingguo, Yan, Yueming, "Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l." in Genetika, 50, no. 2 (2018):503-517,
https://doi.org/10.2298/GENSR1802503W . .

TY  - JOUR
AU  - Wu, Jisu
AU  - Lu, Xiaobin
AU  - Yu, Zitong
AU  - Han, Caixia
AU  - Li, Xiaohui
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4407
AB  - Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and gliadins through intermolecular disulfide bond, confer dough viscoelasticity and wheat processing quality. Glutenins consist of high and low molecular weight glutenin subunits (HMW-GS, LMW-GS) encoded by Glu-1 and Glu-3 loci, respectively. The allelic variations at both loci have important effects on GMP content and breadmaking quality. In this study, GMP extraction and size-exclusion high performance liquid chromatography (SE-HPLC) separation were optimized, and then applied to investigate the effects of Glu-1 and Glu-3 allelic variations on GMP content using different wheat genotypes, chromosome substitution lines and near-isogenic lines (NILs). The results showed that the optimized GMP extraction and SE-HPLC protocol could obtain a reproducible separation and reliable quantitation of GMP content with small samples. The allelic variations at Glu-1 and Glu-3 were closely related to GMP content. Particularly, Glu-DId encoded 1Dx5+1Dy10 subunits, Glu-A3a and Glu-B3h encoded an abundant LMW-B subunit respectively had positive effects on GMP content and breadmaking quality. The introgress of HMW-GS and LMW-GS into bread wheat from related genomes could significantly increase GMP content, indicating that wheat related species has potential gene resources for breadmaking quality improvement. Our results demonstrated that SE-HPLC could serve as an effective tool for rapid separation and quantitation of GMPs and had potential application for gluten quality screening in the early generations during wheat quality improvement program.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc)
EP  - 691
IS  - 2
SP  - 677
VL  - 49
DO  - 10.2298/GENSR1702677W
ER  - 

@article{
author = "Wu, Jisu and Lu, Xiaobin and Yu, Zitong and Han, Caixia and Li, Xiaohui and Prodanović, Slaven and Yan, Yueming",
year = "2017",
abstract = "Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and gliadins through intermolecular disulfide bond, confer dough viscoelasticity and wheat processing quality. Glutenins consist of high and low molecular weight glutenin subunits (HMW-GS, LMW-GS) encoded by Glu-1 and Glu-3 loci, respectively. The allelic variations at both loci have important effects on GMP content and breadmaking quality. In this study, GMP extraction and size-exclusion high performance liquid chromatography (SE-HPLC) separation were optimized, and then applied to investigate the effects of Glu-1 and Glu-3 allelic variations on GMP content using different wheat genotypes, chromosome substitution lines and near-isogenic lines (NILs). The results showed that the optimized GMP extraction and SE-HPLC protocol could obtain a reproducible separation and reliable quantitation of GMP content with small samples. The allelic variations at Glu-1 and Glu-3 were closely related to GMP content. Particularly, Glu-DId encoded 1Dx5+1Dy10 subunits, Glu-A3a and Glu-B3h encoded an abundant LMW-B subunit respectively had positive effects on GMP content and breadmaking quality. The introgress of HMW-GS and LMW-GS into bread wheat from related genomes could significantly increase GMP content, indicating that wheat related species has potential gene resources for breadmaking quality improvement. Our results demonstrated that SE-HPLC could serve as an effective tool for rapid separation and quantitation of GMPs and had potential application for gluten quality screening in the early generations during wheat quality improvement program.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc)",
pages = "691-677",
number = "2",
volume = "49",
doi = "10.2298/GENSR1702677W"
}

Wu, J., Lu, X., Yu, Z., Han, C., Li, X., Prodanović, S.,& Yan, Y.. (2017). Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc). in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 677-691.
https://doi.org/10.2298/GENSR1702677W

Wu J, Lu X, Yu Z, Han C, Li X, Prodanović S, Yan Y. Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc). in Genetika. 2017;49(2):677-691.
doi:10.2298/GENSR1702677W .

Wu, Jisu, Lu, Xiaobin, Yu, Zitong, Han, Caixia, Li, Xiaohui, Prodanović, Slaven, Yan, Yueming, "Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc)" in Genetika, 49, no. 2 (2017):677-691,
https://doi.org/10.2298/GENSR1702677W . .

TY  - JOUR
AU  - Dong, Liwei
AU  - Li, Ning
AU  - Lu, Xiaobing
AU  - Prodanović, Slaven
AU  - Xu, Yanhao
AU  - Zhang, Wenying
AU  - Yan, Yueming
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4191
AB  - Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines
EP  - 269
IS  - 1
SP  - 249
VL  - 48
DO  - 10.2298/GENSR1601249D
ER  - 

@article{
author = "Dong, Liwei and Li, Ning and Lu, Xiaobing and Prodanović, Slaven and Xu, Yanhao and Zhang, Wenying and Yan, Yueming",
year = "2016",
abstract = "Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines",
pages = "269-249",
number = "1",
volume = "48",
doi = "10.2298/GENSR1601249D"
}

Dong, L., Li, N., Lu, X., Prodanović, S., Xu, Y., Zhang, W.,& Yan, Y.. (2016). Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 249-269.
https://doi.org/10.2298/GENSR1601249D

Dong L, Li N, Lu X, Prodanović S, Xu Y, Zhang W, Yan Y. Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika. 2016;48(1):249-269.
doi:10.2298/GENSR1601249D .

Dong, Liwei, Li, Ning, Lu, Xiaobing, Prodanović, Slaven, Xu, Yanhao, Zhang, Wenying, Yan, Yueming, "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines" in Genetika, 48, no. 1 (2016):249-269,
https://doi.org/10.2298/GENSR1601249D . .