TY  - JOUR
AU  - Supramani, Sugenendran
AU  - Ahmad, Rahayu
AU  - Ilham, Zul
AU  - Annuar, Mohamad Suffian Mohamad
AU  - Klaus, Anita
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5018
AB  - Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p  lt  0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p  lt  0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.
PB  - Amer Inst Mathematical Sciences-Aims, Springfield
T2  - Aims Microbiology
T1  - Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology
EP  - 38
IS  - 1
SP  - 19
VL  - 5
DO  - 10.3934/microbiol.2019.1.19
ER  - 

@article{
author = "Supramani, Sugenendran and Ahmad, Rahayu and Ilham, Zul and Annuar, Mohamad Suffian Mohamad and Klaus, Anita and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p  lt  0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p  lt  0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum.",
publisher = "Amer Inst Mathematical Sciences-Aims, Springfield",
journal = "Aims Microbiology",
title = "Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology",
pages = "38-19",
number = "1",
volume = "5",
doi = "10.3934/microbiol.2019.1.19"
}

Supramani, S., Ahmad, R., Ilham, Z., Annuar, M. S. M., Klaus, A.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology. in Aims Microbiology
Amer Inst Mathematical Sciences-Aims, Springfield., 5(1), 19-38.
https://doi.org/10.3934/microbiol.2019.1.19

Supramani S, Ahmad R, Ilham Z, Annuar MSM, Klaus A, Wan-Mohtar WAAQI. Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology. in Aims Microbiology. 2019;5(1):19-38.
doi:10.3934/microbiol.2019.1.19 .

Supramani, Sugenendran, Ahmad, Rahayu, Ilham, Zul, Annuar, Mohamad Suffian Mohamad, Klaus, Anita, Wan-Mohtar, Wan Abd Al Qadr Imad, "Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology" in Aims Microbiology, 5, no. 1 (2019):19-38,
https://doi.org/10.3934/microbiol.2019.1.19 . .