TY  - JOUR
AU  - Hassan, Nurul Amirah
AU  - Supramani, Sugenendran
AU  - Sohedein, Mohamad Nor Azzimi
AU  - Usuldin, Siti Rokhiyah Ahmad
AU  - Klaus, Anita
AU  - Ilham, Zul
AU  - Chen, Wei-Hsin
AU  - Wan-Mohtar, Wan Abd Al Qadr Imad
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5007
AB  - A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p  lt  0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.
PB  - Elsevier, Amsterdam
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation
VL  - 21
DO  - 10.1016/j.bcab.2019.101305
ER  - 

@article{
author = "Hassan, Nurul Amirah and Supramani, Sugenendran and Sohedein, Mohamad Nor Azzimi and Usuldin, Siti Rokhiyah Ahmad and Klaus, Anita and Ilham, Zul and Chen, Wei-Hsin and Wan-Mohtar, Wan Abd Al Qadr Imad",
year = "2019",
abstract = "A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Ayala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642.8 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (K-nuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4-6), temperature (20 degrees C-30 degrees C), and glucose concentration (10 g/L-50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p  lt  0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30 degrees C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.",
publisher = "Elsevier, Amsterdam",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation",
volume = "21",
doi = "10.1016/j.bcab.2019.101305"
}

Hassan, N. A., Supramani, S., Sohedein, M. N. A., Usuldin, S. R. A., Klaus, A., Ilham, Z., Chen, W.,& Wan-Mohtar, W. A. A. Q. I.. (2019). Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology
Elsevier, Amsterdam., 21.
https://doi.org/10.1016/j.bcab.2019.101305

Hassan NA, Supramani S, Sohedein MNA, Usuldin SRA, Klaus A, Ilham Z, Chen W, Wan-Mohtar WAAQI. Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation. in Biocatalysis and Agricultural Biotechnology. 2019;21.
doi:10.1016/j.bcab.2019.101305 .

Hassan, Nurul Amirah, Supramani, Sugenendran, Sohedein, Mohamad Nor Azzimi, Usuldin, Siti Rokhiyah Ahmad, Klaus, Anita, Ilham, Zul, Chen, Wei-Hsin, Wan-Mohtar, Wan Abd Al Qadr Imad, "Efficient biomass-exopolysaccharide production from an identified wild-Serbian Ganoderma lucidum strain BGF4A1 mycelium in a controlled submerged fermentation" in Biocatalysis and Agricultural Biotechnology, 21 (2019),
https://doi.org/10.1016/j.bcab.2019.101305 . .