TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Jevdjović, Radosav
AU  - Zivković, Jelena
AU  - Razić, Slavica
AU  - Moravčević, Djordje
AU  - Maksimović, Zoran
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5039
AB  - In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.
EP  - 169
SP  - 162
VL  - 130
DO  - 10.1016/j.indcrop.2018.12.055
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Jevdjović, Radosav and Zivković, Jelena and Razić, Slavica and Moravčević, Djordje and Maksimović, Zoran",
year = "2019",
abstract = "In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.",
pages = "169-162",
volume = "130",
doi = "10.1016/j.indcrop.2018.12.055"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Jevdjović, R., Zivković, J., Razić, S., Moravčević, D.,& Maksimović, Z.. (2019). Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 130, 162-169.
https://doi.org/10.1016/j.indcrop.2018.12.055

Arsenijević J, Drobac M, Šoštarić I, Jevdjović R, Zivković J, Razić S, Moravčević D, Maksimović Z. Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products. 2019;130:162-169.
doi:10.1016/j.indcrop.2018.12.055 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Jevdjović, Radosav, Zivković, Jelena, Razić, Slavica, Moravčević, Djordje, Maksimović, Zoran, "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All." in Industrial Crops and Products, 130 (2019):162-169,
https://doi.org/10.1016/j.indcrop.2018.12.055 . .

TY  - JOUR
AU  - Cebović, Tatjana
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Zivković, Jelena
AU  - Šoštarić, Ivan
AU  - Maksimović, Zoran
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4740
AB  - Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent
EP  - 567
IS  - 2
SP  - 560
VL  - 55
DO  - 10.1007/s13197-017-2965-1
ER  - 

@article{
author = "Cebović, Tatjana and Arsenijević, Jelena and Drobac, Milica and Zivković, Jelena and Šoštarić, Ivan and Maksimović, Zoran",
year = "2018",
abstract = "Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent",
pages = "567-560",
number = "2",
volume = "55",
doi = "10.1007/s13197-017-2965-1"
}

Cebović, T., Arsenijević, J., Drobac, M., Zivković, J., Šoštarić, I.,& Maksimović, Z.. (2018). Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 55(2), 560-567.
https://doi.org/10.1007/s13197-017-2965-1

Cebović T, Arsenijević J, Drobac M, Zivković J, Šoštarić I, Maksimović Z. Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore. 2018;55(2):560-567.
doi:10.1007/s13197-017-2965-1 .

Cebović, Tatjana, Arsenijević, Jelena, Drobac, Milica, Zivković, Jelena, Šoštarić, Ivan, Maksimović, Zoran, "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent" in Journal of Food Science and Technology-Mysore, 55, no. 2 (2018):560-567,
https://doi.org/10.1007/s13197-017-2965-1 . .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Razić, Slavica
AU  - Milenković, Marina
AU  - Couladis, Maria
AU  - Maksimović, Zoran
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4027
AB  - Hungarian thyme (Thymus pannonicus All., Lamiaceae) is an aromatic herb used as traditional remedy, a refreshing beverage and a food aromatizer. Herbal teas, i.e., infusions, of Hungarian thyme from eight localities in Serbia were analyzed regarding their polyphenolic and volatile composition, and tested for their antioxidant and antimicrobial activity. The total polyphenolics content, determined by the Folin-Ciocalteu method, ranged from 1122.25 to 1979.93 mg gallic acid/L. HPLC analysis revealed rosmarinic acid (367.42-1199.47 mg/L) and luteolin glucuronides as the main polyphenolics. The volatile fractions of the infusions, analyzed by static headspace extraction coupled with GC and GC-MS analyses, contained citral, 3-octanone, 1-octen-3-ol, linalool and 1,8-cineole as the dominant constituents. The antioxidant activity of the infusions was examined through the ferric reducing antioxidant power (FRAP) (68.09-124.58 mmol Fe2+/L) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests (SC50 1.32-2.96 mu L/mL). The antimicrobial activity was tested by the broth microdilution method against standard strains of Gram(+) Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Enterococcus faecalis and Gram(-) bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and one strain of yeast Candida albicans. The infusions inhibited microbial growth in the tested concentration range (31.25-500.00 mu L/mL) and the strongest activity was exhibited against the strain of C. albicans (MIC 31.25-62.50 mu L/mL). The antioxidant and antimicrobial properties of the infusions were, to some extent, in correlation with the composition and content of the polyphenolic compounds, whereas the volatiles noticeably influenced the exhibited antimicrobial activity.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics
EP  - 20
SP  - 14
VL  - 89
DO  - 10.1016/j.indcrop.2016.04.046
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Razić, Slavica and Milenković, Marina and Couladis, Maria and Maksimović, Zoran",
year = "2016",
abstract = "Hungarian thyme (Thymus pannonicus All., Lamiaceae) is an aromatic herb used as traditional remedy, a refreshing beverage and a food aromatizer. Herbal teas, i.e., infusions, of Hungarian thyme from eight localities in Serbia were analyzed regarding their polyphenolic and volatile composition, and tested for their antioxidant and antimicrobial activity. The total polyphenolics content, determined by the Folin-Ciocalteu method, ranged from 1122.25 to 1979.93 mg gallic acid/L. HPLC analysis revealed rosmarinic acid (367.42-1199.47 mg/L) and luteolin glucuronides as the main polyphenolics. The volatile fractions of the infusions, analyzed by static headspace extraction coupled with GC and GC-MS analyses, contained citral, 3-octanone, 1-octen-3-ol, linalool and 1,8-cineole as the dominant constituents. The antioxidant activity of the infusions was examined through the ferric reducing antioxidant power (FRAP) (68.09-124.58 mmol Fe2+/L) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests (SC50 1.32-2.96 mu L/mL). The antimicrobial activity was tested by the broth microdilution method against standard strains of Gram(+) Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Enterococcus faecalis and Gram(-) bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and one strain of yeast Candida albicans. The infusions inhibited microbial growth in the tested concentration range (31.25-500.00 mu L/mL) and the strongest activity was exhibited against the strain of C. albicans (MIC 31.25-62.50 mu L/mL). The antioxidant and antimicrobial properties of the infusions were, to some extent, in correlation with the composition and content of the polyphenolic compounds, whereas the volatiles noticeably influenced the exhibited antimicrobial activity.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics",
pages = "20-14",
volume = "89",
doi = "10.1016/j.indcrop.2016.04.046"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Razić, S., Milenković, M., Couladis, M.,& Maksimović, Z.. (2016). Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics. in Industrial Crops and Products
Elsevier, Amsterdam., 89, 14-20.
https://doi.org/10.1016/j.indcrop.2016.04.046

Arsenijević J, Drobac M, Šoštarić I, Razić S, Milenković M, Couladis M, Maksimović Z. Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics. in Industrial Crops and Products. 2016;89:14-20.
doi:10.1016/j.indcrop.2016.04.046 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Razić, Slavica, Milenković, Marina, Couladis, Maria, Maksimović, Zoran, "Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics" in Industrial Crops and Products, 89 (2016):14-20,
https://doi.org/10.1016/j.indcrop.2016.04.046 . .

TY  - JOUR
AU  - Jug-Dujaković, Marija
AU  - Ristić, Mihailo
AU  - Pljevljakusić, Dejan
AU  - Dajić-Stevanović, Zora
AU  - Liber, Zlatko
AU  - Hancević, Katarina
AU  - Radić, Tomislav
AU  - Satović, Zlatko
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2976
AB  - Essential oils of 25 indigenous populations of Dalmatian sage (Salvia officinalis L.) that represent nearly half of native distribution area of the species were analyzed. Plantlets collected from wild populations were grown in the same field under the same environmental conditions and then sampled for essential-oil analysis. The yield of essential oil ranged from 1.93 to 3.70% with average of 2.83%. Among the 62 compounds detected, eight (cis-thujone, camphor, trans-thujone, 1,8-cineole, beta-pinene, camphene, borneol, and bornyl acetate) formed 78.1387.33% of essential oils of individual populations. Strong positive correlations were observed between camphor and beta-pinene,beta-pinene and borneol, as well as between borneol and bornyl acetate. The strongest negative correlation was detected between camphor and trans-thujone. Principal component analysis (PCA) on the basis of eight main compounds showed that first main component separated populations with high thujone content, from those rich in camphor, while the second component separated populations rich in cis-thujone from those rich in trans-thujone. Cluster analysis (CA) led to the identification of three chemotypes of S. officinalis populations: cis-thujone; trans-tujone, and camphor/beta-pinene/borneol/bornyl acetate. We propose that differences in essential oils of 25 populations are mostly genetically controlled, since potential environmental factors were controlled in this study.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition
EP  - 2323
IS  - 10
SP  - 2309
VL  - 9
DO  - 10.1002/cbdv.201200131
ER  - 

@article{
author = "Jug-Dujaković, Marija and Ristić, Mihailo and Pljevljakusić, Dejan and Dajić-Stevanović, Zora and Liber, Zlatko and Hancević, Katarina and Radić, Tomislav and Satović, Zlatko",
year = "2012",
abstract = "Essential oils of 25 indigenous populations of Dalmatian sage (Salvia officinalis L.) that represent nearly half of native distribution area of the species were analyzed. Plantlets collected from wild populations were grown in the same field under the same environmental conditions and then sampled for essential-oil analysis. The yield of essential oil ranged from 1.93 to 3.70% with average of 2.83%. Among the 62 compounds detected, eight (cis-thujone, camphor, trans-thujone, 1,8-cineole, beta-pinene, camphene, borneol, and bornyl acetate) formed 78.1387.33% of essential oils of individual populations. Strong positive correlations were observed between camphor and beta-pinene,beta-pinene and borneol, as well as between borneol and bornyl acetate. The strongest negative correlation was detected between camphor and trans-thujone. Principal component analysis (PCA) on the basis of eight main compounds showed that first main component separated populations with high thujone content, from those rich in camphor, while the second component separated populations rich in cis-thujone from those rich in trans-thujone. Cluster analysis (CA) led to the identification of three chemotypes of S. officinalis populations: cis-thujone; trans-tujone, and camphor/beta-pinene/borneol/bornyl acetate. We propose that differences in essential oils of 25 populations are mostly genetically controlled, since potential environmental factors were controlled in this study.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition",
pages = "2323-2309",
number = "10",
volume = "9",
doi = "10.1002/cbdv.201200131"
}

Jug-Dujaković, M., Ristić, M., Pljevljakusić, D., Dajić-Stevanović, Z., Liber, Z., Hancević, K., Radić, T.,& Satović, Z.. (2012). High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 9(10), 2309-2323.
https://doi.org/10.1002/cbdv.201200131

Jug-Dujaković M, Ristić M, Pljevljakusić D, Dajić-Stevanović Z, Liber Z, Hancević K, Radić T, Satović Z. High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition. in Chemistry & Biodiversity. 2012;9(10):2309-2323.
doi:10.1002/cbdv.201200131 .

Jug-Dujaković, Marija, Ristić, Mihailo, Pljevljakusić, Dejan, Dajić-Stevanović, Zora, Liber, Zlatko, Hancević, Katarina, Radić, Tomislav, Satović, Zlatko, "High Diversity of Indigenous Populations of Dalmatian Sage (Salvia officinalis L.) in Essential-Oil Composition" in Chemistry & Biodiversity, 9, no. 10 (2012):2309-2323,
https://doi.org/10.1002/cbdv.201200131 . .