TY  - JOUR
AU  - Matović, Kazimir
AU  - Misić, Dušan
AU  - Karabasil, Nedjeljko
AU  - Nedić, Nebojša
AU  - Dmitrić, Marko
AU  - Jevtić, Goran
AU  - Ćirić, Jelena
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5064
AB  - A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Clostridium botulinum spores in European honey bees from Serbia
EP  - 426
IS  - 3
SP  - 420
VL  - 58
DO  - 10.1080/00218839.2018.1560654
ER  - 

@article{
author = "Matović, Kazimir and Misić, Dušan and Karabasil, Nedjeljko and Nedić, Nebojša and Dmitrić, Marko and Jevtić, Goran and Ćirić, Jelena",
year = "2019",
abstract = "A total of 61 honey bees from different regions of the Republic of Serbia were analyzed for Clostridium botulinum (C. botulinum) spores. The microbiological methods and molecular methods (multiplex PCR/mPCR and PCR method) were utilized to examine multiple subunits of each honey bees samples. The C. botulinum spores in PCR-positive samples were estimated by the most probable number method (MPN). The presence of C. botulinum spores, by applying mPCR and PCR methods, was detected in 1 of the 61 honey bees (1.64%). Using MPN method, the number of spores in positive sample was 110/kg. Detection of C. botulinum spores directly from untreated honey bees, without prior enrichment, is impossible by applying PCR. Using conventional microbiological methods, detection of C. botulinum spores in dead honey bees is not possible without preenrichment. Therefore, conventional, microbiological methods are not suitable for the detection of C. botulinum spores in honey bees. In order to detect C. botulinum spores in honey bees using PCR methods, due to the small and/or unequal distribution of spores in the samples, it is desirable to use multiple subunits/replicates for each sample examined.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Clostridium botulinum spores in European honey bees from Serbia",
pages = "426-420",
number = "3",
volume = "58",
doi = "10.1080/00218839.2018.1560654"
}

Matović, K., Misić, D., Karabasil, N., Nedić, N., Dmitrić, M., Jevtić, G.,& Ćirić, J.. (2019). Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 58(3), 420-426.
https://doi.org/10.1080/00218839.2018.1560654

Matović K, Misić D, Karabasil N, Nedić N, Dmitrić M, Jevtić G, Ćirić J. Clostridium botulinum spores in European honey bees from Serbia. in Journal of Apicultural Research. 2019;58(3):420-426.
doi:10.1080/00218839.2018.1560654 .

Matović, Kazimir, Misić, Dušan, Karabasil, Nedjeljko, Nedić, Nebojša, Dmitrić, Marko, Jevtić, Goran, Ćirić, Jelena, "Clostridium botulinum spores in European honey bees from Serbia" in Journal of Apicultural Research, 58, no. 3 (2019):420-426,
https://doi.org/10.1080/00218839.2018.1560654 . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Ćirić, Jelena
AU  - Kaljević, Vesna
AU  - Nedić, Nebojša
AU  - Jevtić, Goran
AU  - Vasković, Nikola
AU  - Baltić, Milan Ž.
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4792
AB  - Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia
EP  - 14157
IS  - 14
SP  - 14148
VL  - 25
DO  - 10.1007/s11356-018-1659-1
ER  - 

@article{
author = "Matović, Kazimir and Ćirić, Jelena and Kaljević, Vesna and Nedić, Nebojša and Jevtić, Goran and Vasković, Nikola and Baltić, Milan Ž.",
year = "2018",
abstract = "Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, five acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia",
pages = "14157-14148",
number = "14",
volume = "25",
doi = "10.1007/s11356-018-1659-1"
}

Matović, K., Ćirić, J., Kaljević, V., Nedić, N., Jevtić, G., Vasković, N.,& Baltić, M. Ž.. (2018). Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 25(14), 14148-14157.
https://doi.org/10.1007/s11356-018-1659-1

Matović K, Ćirić J, Kaljević V, Nedić N, Jevtić G, Vasković N, Baltić MŽ. Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia. in Environmental Science and Pollution Research. 2018;25(14):14148-14157.
doi:10.1007/s11356-018-1659-1 .

Matović, Kazimir, Ćirić, Jelena, Kaljević, Vesna, Nedić, Nebojša, Jevtić, Goran, Vasković, Nikola, Baltić, Milan Ž., "Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia" in Environmental Science and Pollution Research, 25, no. 14 (2018):14148-14157,
https://doi.org/10.1007/s11356-018-1659-1 . .

TY  - JOUR
AU  - Stanojević, S.
AU  - Valčić, Miroslav
AU  - Stanojević, S.
AU  - Radojicić, S.
AU  - Avramov, Stevan N.
AU  - Tambur, Z.
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3692
AB  - Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard holdings. Elements of the control strategy are based on applicable regulations and include the simulation of detection of the disease, setting up the protected and surveillance zones, standstill of pig movements and restricted movement of animals, vehicles, equipment, and people with strong control measures in protection and surveillance zones, euthanasia of susceptible pigs, protective vaccination of pigs, compensation etc. During the simulation, different output parameters were compared such as: duration of epidemic of a disease, number of affected holdings and animals, direct costs such as those for dead or culled animals, costs of surveillance, disposal of infectious materials, cleaning and disinfection. Depopulation of affected animals with early diagnostics and vaccination in the protection and surveillance zone proved to be the most effective measures to stop the spread and for the eradication of the disease. Moreover, during the simulation, systematic implementation of biosecurity measures in all pig production clusters was demonstrated to be an appropriate strategy for sustainable control of CSF and the establishment of a stable epidemiological situation.
PB  - Czech Academy of Agricultural Sciences
T2  - Veterinarni Medicina
T1  - Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia
EP  - 566
IS  - 10
SP  - 553
VL  - 60
DO  - 10.17221/8494-VETMED
ER  - 

@article{
author = "Stanojević, S. and Valčić, Miroslav and Stanojević, S. and Radojicić, S. and Avramov, Stevan N. and Tambur, Z.",
year = "2015",
abstract = "Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard holdings. Elements of the control strategy are based on applicable regulations and include the simulation of detection of the disease, setting up the protected and surveillance zones, standstill of pig movements and restricted movement of animals, vehicles, equipment, and people with strong control measures in protection and surveillance zones, euthanasia of susceptible pigs, protective vaccination of pigs, compensation etc. During the simulation, different output parameters were compared such as: duration of epidemic of a disease, number of affected holdings and animals, direct costs such as those for dead or culled animals, costs of surveillance, disposal of infectious materials, cleaning and disinfection. Depopulation of affected animals with early diagnostics and vaccination in the protection and surveillance zone proved to be the most effective measures to stop the spread and for the eradication of the disease. Moreover, during the simulation, systematic implementation of biosecurity measures in all pig production clusters was demonstrated to be an appropriate strategy for sustainable control of CSF and the establishment of a stable epidemiological situation.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Veterinarni Medicina",
title = "Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia",
pages = "566-553",
number = "10",
volume = "60",
doi = "10.17221/8494-VETMED"
}

Stanojević, S., Valčić, M., Stanojević, S., Radojicić, S., Avramov, S. N.,& Tambur, Z.. (2015). Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia. in Veterinarni Medicina
Czech Academy of Agricultural Sciences., 60(10), 553-566.
https://doi.org/10.17221/8494-VETMED

Stanojević S, Valčić M, Stanojević S, Radojicić S, Avramov SN, Tambur Z. Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia. in Veterinarni Medicina. 2015;60(10):553-566.
doi:10.17221/8494-VETMED .

Stanojević, S., Valčić, Miroslav, Stanojević, S., Radojicić, S., Avramov, Stevan N., Tambur, Z., "Simulation of a classical swine fever outbreak in rural areas of the Republic of Serbia" in Veterinarni Medicina, 60, no. 10 (2015):553-566,
https://doi.org/10.17221/8494-VETMED . .