@misc{
author = "Sknepnek, Aleksandra and Filipović, Suzana and Pavlović, Vladimir B. and Mirković, Nemanja and Miletić, Dunja and Pantić, Milena and Mirković, Miljana",
year = "2024",
abstract = "Acetic acid bacteria (AAB) are renowned for their proficiency in bacterial cellulose (BC) production, particularly species within the Acetobacter and Komagataeibacter genera. BC, derived solely from bacterial cells, represents the purest form of cellulose known, with AAB capable of generating quantities viable for commercial utilization. The utilization of bacteria for biopolymer or cellulose hydrogel production, as opposed to traditional plant sources requiring aggressive chemical treatments, presents a sustainable approach with environmental preservation benefits.
While BC materials inherently lack functional properties, their porous structure and three-dimensional nanofiber network with high specific surface area render them ideal carriers for antimicrobials or other agents in the production of functional composite materials. Moreover, by-products or wastes that contain carbon or nitrogen sources for AAB could potentially substitute some part of conventional substrates, reducing production costs of BC and conserving resources.
This study involved the identification of eight isolates from two kombucha beverages using molecular method, followed by screening for the most proficient cellulose-producing species. Morphological characterization of cellulose was conducted using scanning electron microscopy (SEM), while X-ray diffraction (XRD) was employed for crystallinity and phase analysis. Functionalization of nanocellulose involved the incorporation of titanium dioxide and hydroxyapatite, with elemental composition analyzed using energy-dispersive X-ray (EDS) techniques. Antimicrobial properties were evaluated using plate count tests. Additionally, discarded ethanol waste from the functionalization step was investigated for sustainable BC cellulose production.
Isolated species demonstrated the production of pure, nanosized, densely intertwined BC polymers with high crystallinity. EDS analysis confirmed the presence of only carbon and oxygen elements in pure cellulose. While pure BC exhibited no antimicrobial activity, functionalized BC demonstrated antifungal and antibacterial properties. Furthermore, discarded ethanol waste proved effective for BC synthesis. These findings underscore the potential applications of functionalized BC in touch surfaces, coatings, packaging, agriculture, and medicine.",
publisher = "Serbian Society for Microbiology",
journal = "XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA, MICROMED REGIO 5",
title = "ACETIC ACID BACTERIA-DERIVED BACTERIAL NANOCELLULOSE: SUSTAINABLE SYNTHESIS AND ANTIMICROBIAL POTENTIAL DEVELOPMENT",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6948"
}
