TY  - JOUR
AU  - Meland, Mekjell
AU  - Froynes, Oddmund
AU  - Fotirić-Akšić, Milica
AU  - Pojskić, Naris
AU  - Kalamujić-Stroil, Belma
AU  - Lasić, Lejla
AU  - Gasi, Fuad
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5335
AB  - European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars 'Edda', 'Opal' (self-fertile), 'Jubileum', 'Reeves', 'Mallard', 'Avalon', 'Cacanska Lepotica' (self-fertile), and 'Valor', 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that 'Opal' was the most successful pollinizer among the investigated plum cultivars. The main exception was 'Cacanska Lepotica', which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of 'Opal' was 'Mallard'. However, in more than two thirds of embryos extracted from 'Opal' fruits self-fertilization was determined. 'Reeves' was identified as the most successful pollinizer among embryos collected from 'Valor'. Among the five cultivars ('Edda', 'Jubileum', 'Reeves', 'Mallard', and 'Avalon') that did not display self-pollination, the pollinizer success rate of 'Opal', ranged from 36.5% ('Mallard') to 93.5% ('Edda') in 2017, while in 2018 this rate ranged from 43.5% ('Jubileum' and 'Reeves') up to 96.5% ('Edda'). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers
IS  - 2
VL  - 10
DO  - 10.3390/agronomy10020264
ER  - 

@article{
author = "Meland, Mekjell and Froynes, Oddmund and Fotirić-Akšić, Milica and Pojskić, Naris and Kalamujić-Stroil, Belma and Lasić, Lejla and Gasi, Fuad",
year = "2020",
abstract = "European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars 'Edda', 'Opal' (self-fertile), 'Jubileum', 'Reeves', 'Mallard', 'Avalon', 'Cacanska Lepotica' (self-fertile), and 'Valor', 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that 'Opal' was the most successful pollinizer among the investigated plum cultivars. The main exception was 'Cacanska Lepotica', which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of 'Opal' was 'Mallard'. However, in more than two thirds of embryos extracted from 'Opal' fruits self-fertilization was determined. 'Reeves' was identified as the most successful pollinizer among embryos collected from 'Valor'. Among the five cultivars ('Edda', 'Jubileum', 'Reeves', 'Mallard', and 'Avalon') that did not display self-pollination, the pollinizer success rate of 'Opal', ranged from 36.5% ('Mallard') to 93.5% ('Edda') in 2017, while in 2018 this rate ranged from 43.5% ('Jubileum' and 'Reeves') up to 96.5% ('Edda'). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers",
number = "2",
volume = "10",
doi = "10.3390/agronomy10020264"
}

Meland, M., Froynes, O., Fotirić-Akšić, M., Pojskić, N., Kalamujić-Stroil, B., Lasić, L.,& Gasi, F.. (2020). Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers. in Agronomy-Basel
MDPI, BASEL., 10(2).
https://doi.org/10.3390/agronomy10020264

Meland M, Froynes O, Fotirić-Akšić M, Pojskić N, Kalamujić-Stroil B, Lasić L, Gasi F. Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers. in Agronomy-Basel. 2020;10(2).
doi:10.3390/agronomy10020264 .

Meland, Mekjell, Froynes, Oddmund, Fotirić-Akšić, Milica, Pojskić, Naris, Kalamujić-Stroil, Belma, Lasić, Lejla, Gasi, Fuad, "Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers" in Agronomy-Basel, 10, no. 2 (2020),
https://doi.org/10.3390/agronomy10020264 . .