Research Council of NorwayResearch Council of Norway [244510]

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Research Council of NorwayResearch Council of Norway [244510]

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S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'

Gasi, Fuad; Froynes, Oddmund; Kalamujić-Stroil, Belma; Lasić, Lejla; Pojskić, Naris; Fotirić-Akšić, Milica; Meland, Mekjell

(MDPI, BASEL, 2020)

TY  - JOUR
AU  - Gasi, Fuad
AU  - Froynes, Oddmund
AU  - Kalamujić-Stroil, Belma
AU  - Lasić, Lejla
AU  - Pojskić, Naris
AU  - Fotirić-Akšić, Milica
AU  - Meland, Mekjell
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5291
AB  - The diploid Celina/QTee(R)('Coloree de Juillet' x 'Williams'), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green BACKGROUND: . Although, 'Celina' is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine theS-alleles of 'Celina', as well as its frequently used pollinizers, and, through paternity testing of 'Celina' seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, 'Celina' and its potential pollinizers were allS-genotyped. After harvest, seeds collected from 'Celina' fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from 'Celina', as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that 'Anna', the only examined pollinizer that was fully cross-compatible with 'Celina', together with 'Fritjof', the genotype which had the highest flowering overlap with 'Celina', proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated 'Celina' orchards.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'
IS  - 9
VL  - 10
DO  - 10.3390/agronomy10091372
ER  - 
@article{
author = "Gasi, Fuad and Froynes, Oddmund and Kalamujić-Stroil, Belma and Lasić, Lejla and Pojskić, Naris and Fotirić-Akšić, Milica and Meland, Mekjell",
year = "2020",
abstract = "The diploid Celina/QTee(R)('Coloree de Juillet' x 'Williams'), one of the most promising pear cultivars developed by the Norwegian breeding program Graminor, was launched in 2010. In Norway, the flowering is medium to late, while the fruits ripen in the beginning of September. The fruits are attractive with an intense red blush (50%) on a green BACKGROUND: . Although, 'Celina' is cultivated in the most climatically suitable regions for fruit cultivation, present in Norway, unfavorable environmental conditions for pear pollination can have a very negative effect on fruit set and consequent yield. The aim of this study was to determine theS-alleles of 'Celina', as well as its frequently used pollinizers, and, through paternity testing of 'Celina' seeds, give a recommendation regarding the most important pollinizers of this pear cultivar. In order to accomplish this, 'Celina' and its potential pollinizers were allS-genotyped. After harvest, seeds collected from 'Celina' fruit in 2017 and 2018 were genotyped using eleven microsatellite markers. Genomic DNA was also extracted from leaf material collected from 'Celina', as well as from five pear cultivars used as pollinizers in the three examined orchards, and analyzed using the same marker set. Subsequently a simple sequence repeat (SSR) database was constructed and used for gene assignment analyses with the aim of quantifying pollen donor contribution from individual pollinizers. The obtained results indicate that 'Anna', the only examined pollinizer that was fully cross-compatible with 'Celina', together with 'Fritjof', the genotype which had the highest flowering overlap with 'Celina', proved to be the most successful pollinizers across all seasons and orchards. Although both cultivars were ubiquitous in the examined orchards, either as planted trees or as branches introduced during the flowering period, they were the most abundant pollinizers in only one orchard each. It is therefore possible to conclude that pollinizer abundance has a secondary significance in pollinizer success within investigated 'Celina' orchards.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'",
number = "9",
volume = "10",
doi = "10.3390/agronomy10091372"
}
Gasi, F., Froynes, O., Kalamujić-Stroil, B., Lasić, L., Pojskić, N., Fotirić-Akšić, M.,& Meland, M.. (2020). S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'. in Agronomy-Basel
MDPI, BASEL., 10(9).
https://doi.org/10.3390/agronomy10091372
Gasi F, Froynes O, Kalamujić-Stroil B, Lasić L, Pojskić N, Fotirić-Akšić M, Meland M. S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'. in Agronomy-Basel. 2020;10(9).
doi:10.3390/agronomy10091372 .
Gasi, Fuad, Froynes, Oddmund, Kalamujić-Stroil, Belma, Lasić, Lejla, Pojskić, Naris, Fotirić-Akšić, Milica, Meland, Mekjell, "S-Genotyping and Seed Paternity Testing of the Pear Cultivar 'Celina'" in Agronomy-Basel, 10, no. 9 (2020),
https://doi.org/10.3390/agronomy10091372 . .
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Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate

Cerović, Radosav; Fotirić-Akšić, Milica; Meland, Mekjell

(MDPI, BASEL, 2020)

TY  - JOUR
AU  - Cerović, Radosav
AU  - Fotirić-Akšić, Milica
AU  - Meland, Mekjell
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5249
AB  - Norwegian pear production is low due to climatic limitations, a lack of well-adapted cultivars and suitable pollinizers. However, nowadays it is increasing as a result of newly introduced and bred pear cultivars. Since cross pollination is necessary for high yields and good fruit quality, the aim of this investigation was to find the most suitable pollinizers for the pear cultivars "Ingeborg" ("Conference" x "Bonne Louise") and "Celina" ("Coloree de Juillet" x "Williams"). Self-pollination of "Ingeborg" and "Celina", together with "Conference", "Belle Lucrative", "Anna", "Clara Frijs", "Herzogin Elsa", "Kristina" and "Fritjof" as potential pollinizers, were studied in this experiment during the 2017 and 2018 seasons in Norway. The success rate of each pollinizer was tested under field conditions, while the monitoring of pollen tube growth was done using the fluorescence microscopy method. All reproductive parameters (pollen germination, number of pollen tubes in the upper part of the style, pollen tube number in the locule of the ovary, number of fertilized ovules, initial fruit set, and final fruit set) in all crossing combinations were higher in 2018 due to much warmer weather. Based on the flowering overlap and success rate of each individual pollinizer and fruit set, the cultivars "Anna" and "Clara Frijs" can be suggested as pollinizers for the cultivar "Ingeborg", while "Fritjof", "Anna", "Kristina" and "Herzogin Elsa" for the cultivar "Celina". An even distribution of two compatible pollinizers having overlapping flowering times with the main commercial pear cultivar is a general recommendation for commercial pear production.
PB  - MDPI, BASEL
T2  - Agronomy-Basel
T1  - Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate
IS  - 7
VL  - 10
DO  - 10.3390/agronomy10070970
ER  - 
@article{
author = "Cerović, Radosav and Fotirić-Akšić, Milica and Meland, Mekjell",
year = "2020",
abstract = "Norwegian pear production is low due to climatic limitations, a lack of well-adapted cultivars and suitable pollinizers. However, nowadays it is increasing as a result of newly introduced and bred pear cultivars. Since cross pollination is necessary for high yields and good fruit quality, the aim of this investigation was to find the most suitable pollinizers for the pear cultivars "Ingeborg" ("Conference" x "Bonne Louise") and "Celina" ("Coloree de Juillet" x "Williams"). Self-pollination of "Ingeborg" and "Celina", together with "Conference", "Belle Lucrative", "Anna", "Clara Frijs", "Herzogin Elsa", "Kristina" and "Fritjof" as potential pollinizers, were studied in this experiment during the 2017 and 2018 seasons in Norway. The success rate of each pollinizer was tested under field conditions, while the monitoring of pollen tube growth was done using the fluorescence microscopy method. All reproductive parameters (pollen germination, number of pollen tubes in the upper part of the style, pollen tube number in the locule of the ovary, number of fertilized ovules, initial fruit set, and final fruit set) in all crossing combinations were higher in 2018 due to much warmer weather. Based on the flowering overlap and success rate of each individual pollinizer and fruit set, the cultivars "Anna" and "Clara Frijs" can be suggested as pollinizers for the cultivar "Ingeborg", while "Fritjof", "Anna", "Kristina" and "Herzogin Elsa" for the cultivar "Celina". An even distribution of two compatible pollinizers having overlapping flowering times with the main commercial pear cultivar is a general recommendation for commercial pear production.",
publisher = "MDPI, BASEL",
journal = "Agronomy-Basel",
title = "Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate",
number = "7",
volume = "10",
doi = "10.3390/agronomy10070970"
}
Cerović, R., Fotirić-Akšić, M.,& Meland, M.. (2020). Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate. in Agronomy-Basel
MDPI, BASEL., 10(7).
https://doi.org/10.3390/agronomy10070970
Cerović R, Fotirić-Akšić M, Meland M. Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate. in Agronomy-Basel. 2020;10(7).
doi:10.3390/agronomy10070970 .
Cerović, Radosav, Fotirić-Akšić, Milica, Meland, Mekjell, "Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate" in Agronomy-Basel, 10, no. 7 (2020),
https://doi.org/10.3390/agronomy10070970 . .
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