TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5315
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Turnip Yellows Virus on Cabbage in Serbia
EP  - 998
IS  - 3
SP  - 997
VL  - 104
DO  - 10.1094/PDIS-08-19-1682-PDN
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Stanković, Ivana and Krstić, Branka",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Turnip Yellows Virus on Cabbage in Serbia",
pages = "998-997",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1682-PDN"
}

Milošević, D., Ignjatov, M., Vlajić, S., Nikolić, Z., Gvozdanović-Varga, J., Stanković, I.,& Krstić, B.. (2020). First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(3), 997-998.
https://doi.org/10.1094/PDIS-08-19-1682-PDN

Milošević D, Ignjatov M, Vlajić S, Nikolić Z, Gvozdanović-Varga J, Stanković I, Krstić B. First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease. 2020;104(3):997-998.
doi:10.1094/PDIS-08-19-1682-PDN .

Milošević, Dragana, Ignjatov, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Stanković, Ivana, Krstić, Branka, "First Report of Turnip Yellows Virus on Cabbage in Serbia" in Plant Disease, 104, no. 3 (2020):997-998,
https://doi.org/10.1094/PDIS-08-19-1682-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Krstić, Branka
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5234
AB  - In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
AB  - Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
T1  - Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
EP  - 87
IS  - 3
SP  - 82
VL  - 56
DO  - 10.5937/ratpov56-23514
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Stanković, Ivana and Nikolić, Zorica and Tamindžić, Gordana and Krstić, Branka",
year = "2019",
abstract = "In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank., Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia, Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji",
pages = "87-82",
number = "3",
volume = "56",
doi = "10.5937/ratpov56-23514"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Stanković, I., Nikolić, Z., Tamindžić, G.,& Krstić, B.. (2019). Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 56(3), 82-87.
https://doi.org/10.5937/ratpov56-23514

Milošević D, Ignjatov M, Marjanović-Jeromela A, Stanković I, Nikolić Z, Tamindžić G, Krstić B. Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo. 2019;56(3):82-87.
doi:10.5937/ratpov56-23514 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Stanković, Ivana, Nikolić, Zorica, Tamindžić, Gordana, Krstić, Branka, "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia" in Ratarstvo i povrtarstvo, 56, no. 3 (2019):82-87,
https://doi.org/10.5937/ratpov56-23514 . .

TY  - JOUR
AU  - Nikšić, Dragan
AU  - Pantelić, Vlada
AU  - Ostojić-Andrić, Dušica
AU  - Perišić, Predrag
AU  - Petricević, Veselin
AU  - Lazarević, Marina
AU  - Petricević, Maja
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4661
AB  - The objective of this study was to determine the frequency of the kappa-casein and beta-lactoglobulin genotypes in the Simmental cattle in Serbia and compare it with the frequency according to the Hardy-Weinberg equilibrium law. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Of the 157 cows included in this study, the AA kappa-casein genotype was found in 53 cows, which makes a frequency of 33.80%, the AB genotype in 81 cows or 51.60% and the BB genotype in 23 cows or 14.60%. The allelic frequency A was 59.60%, while allele B had a frequency of 40.40%. In regard to the frequency of genotypes and beta-lactoglobulin alleles for the total studied population of cows obtained for AA, AB and BB genotypes for beta-lactoglobulin, was 33.10%, 49.70% and 17.20%, respectively, which means that 52 animals had genotype AA, 78 genotype AB and 27 genotype BB. The frequency of alleles A and B resulting from the incidence of genotypes was 58.00% for allele A and 42.00% for allele B. The specified frequencies for both protein fractions statistically differed significantly from the frequency according to the Hardy-Weinberg equilibrium law, which confirmed the absence of equilibrium in the examined population.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia
EP  - 668
IS  - 2
SP  - 659
VL  - 50
DO  - 10.2298/GENSR1802659N
ER  - 

@article{
author = "Nikšić, Dragan and Pantelić, Vlada and Ostojić-Andrić, Dušica and Perišić, Predrag and Petricević, Veselin and Lazarević, Marina and Petricević, Maja",
year = "2018",
abstract = "The objective of this study was to determine the frequency of the kappa-casein and beta-lactoglobulin genotypes in the Simmental cattle in Serbia and compare it with the frequency according to the Hardy-Weinberg equilibrium law. Blood samples were taken from a total of 157 cows of the Simmental breed in Toplica and Rasina districts. Of the 157 cows included in this study, the AA kappa-casein genotype was found in 53 cows, which makes a frequency of 33.80%, the AB genotype in 81 cows or 51.60% and the BB genotype in 23 cows or 14.60%. The allelic frequency A was 59.60%, while allele B had a frequency of 40.40%. In regard to the frequency of genotypes and beta-lactoglobulin alleles for the total studied population of cows obtained for AA, AB and BB genotypes for beta-lactoglobulin, was 33.10%, 49.70% and 17.20%, respectively, which means that 52 animals had genotype AA, 78 genotype AB and 27 genotype BB. The frequency of alleles A and B resulting from the incidence of genotypes was 58.00% for allele A and 42.00% for allele B. The specified frequencies for both protein fractions statistically differed significantly from the frequency according to the Hardy-Weinberg equilibrium law, which confirmed the absence of equilibrium in the examined population.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia",
pages = "668-659",
number = "2",
volume = "50",
doi = "10.2298/GENSR1802659N"
}

Nikšić, D., Pantelić, V., Ostojić-Andrić, D., Perišić, P., Petricević, V., Lazarević, M.,& Petricević, M.. (2018). Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 50(2), 659-668.
https://doi.org/10.2298/GENSR1802659N

Nikšić D, Pantelić V, Ostojić-Andrić D, Perišić P, Petricević V, Lazarević M, Petricević M. Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia. in Genetika. 2018;50(2):659-668.
doi:10.2298/GENSR1802659N .

Nikšić, Dragan, Pantelić, Vlada, Ostojić-Andrić, Dušica, Perišić, Predrag, Petricević, Veselin, Lazarević, Marina, Petricević, Maja, "Polymorphism of k-casein and b-lactoglobuline in simmental cattle in Serbia" in Genetika, 50, no. 2 (2018):659-668,
https://doi.org/10.2298/GENSR1802659N . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4880
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Occurrence and diversity of viruses infecting pepper in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 155
IS  - 46
SP  - 141
VL  - 23
DO  - 10.5937/AASer1846141M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Stanković, Ivana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Krstić, Branka",
year = "2018",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Occurrence and diversity of viruses infecting pepper in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "155-141",
number = "46",
volume = "23",
doi = "10.5937/AASer1846141M"
}

Milošević, D., Ignjatov, M., Stanković, I., Nikolić, Z., Gvozdanović-Varga, J.,& Krstić, B.. (2018). Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 23(46), 141-155.
https://doi.org/10.5937/AASer1846141M

Milošević D, Ignjatov M, Stanković I, Nikolić Z, Gvozdanović-Varga J, Krstić B. Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica. 2018;23(46):141-155.
doi:10.5937/AASer1846141M .

Milošević, Dragana, Ignjatov, Maja, Stanković, Ivana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Krstić, Branka, "Occurrence and diversity of viruses infecting pepper in Serbia" in Acta agriculturae Serbica, 23, no. 46 (2018):141-155,
https://doi.org/10.5937/AASer1846141M . .

TY  - JOUR
AU  - Krstić, Branka
AU  - Stanković, Ivana
AU  - Milošević, Dragana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4544
AB  - The number of virus species infecting pepper crops and their incidences has increased considerably over the past 30 years. This is probably due to a combination of factors, including the expansion and intensification of pepper cultivation, the increased global trade of plant material carrying viruses and vectors to new locations, and climate change expanding the geographic range suitable for the viruses and vectors. About 68 viruses are known to infect peppers worldwide. Most pepper viruses are distributed worldwide with the exception of viruses that have been reported only in certain geographic areas. Virus-infected peppers generally cxhibit a variety of symptoms, the most common of which are mosaic, mottle, necrosis and leaf distortion with consequent reductions in crop growth and yield. More than half of known pepper viruses are transmitted by aphids, and the other common pepper viruses are transmitted by whiteflies, thrips, or by contact and/or through the soil. In Serbia, the presence of five viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV) and Tobacco mosaic virus (TMV), with the prevalence of CMV and PVY. Some of these viruses cause considerable yield losses. For each economically important virus in Serbia, control measures are reported here.
AB  - Brojnost virusa koji zaražavaju papriku, kao i učestalost njihove pojave, značajno se povećala poslednjih 30 godina. Uzrok tome je, verovatno, kombinacija različitih faktora, kao što su ekspanzija i intenzivnije gajenje paprike, povećan obim međunarodne trgovine biljnog materijala čime je povećan rizik unošenja virusa i njihovih vektora u nove regione sveta i lokalitete, kao i klimatske promene koje odgovaraju širenju virusa i njihovih vektora. Do sada je utvrđeno da 68 virusa može da zarazi papriku. Većina virusa paprike je raširena celim svetom, ali ima i izuzetaka, koji su prisutni samo u određenim delovima sveta. Virusi paprike izazivaju ispoljavanje različitih tipova simptoma, a najčešći su mozaik, šarenilo i deformacija lišća koji imaju za posledicu smanjenje porasta biljaka i prinosa. Više od polovine virusa paprike se prenosi biljnim vašima, a drugi česti virusi se prenose leptirastim vašima, tripsima ili mehanički. U Srbiji je utvrđeno prisustvo pet virusa: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV) i virus mozaika duvana (Tobacco mosaic virus, TMV), s tim što su prevalentni CMV i PVY. Neki od tih virusa prouzrokuju značajne gubitke u prinosu. Za svaki ekonomski važan virus u Srbiji navedene su mere kontrole.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Economically important virus disease of pepper in Serbia
T1  - Ekonomski značajne viroze paprike u Srbiji
EP  - 627
IS  - 6
SP  - 610
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4544
ER  - 

@article{
author = "Krstić, Branka and Stanković, Ivana and Milošević, Dragana and Vučurović, Ana and Zečević, Katarina",
year = "2017",
abstract = "The number of virus species infecting pepper crops and their incidences has increased considerably over the past 30 years. This is probably due to a combination of factors, including the expansion and intensification of pepper cultivation, the increased global trade of plant material carrying viruses and vectors to new locations, and climate change expanding the geographic range suitable for the viruses and vectors. About 68 viruses are known to infect peppers worldwide. Most pepper viruses are distributed worldwide with the exception of viruses that have been reported only in certain geographic areas. Virus-infected peppers generally cxhibit a variety of symptoms, the most common of which are mosaic, mottle, necrosis and leaf distortion with consequent reductions in crop growth and yield. More than half of known pepper viruses are transmitted by aphids, and the other common pepper viruses are transmitted by whiteflies, thrips, or by contact and/or through the soil. In Serbia, the presence of five viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV) and Tobacco mosaic virus (TMV), with the prevalence of CMV and PVY. Some of these viruses cause considerable yield losses. For each economically important virus in Serbia, control measures are reported here., Brojnost virusa koji zaražavaju papriku, kao i učestalost njihove pojave, značajno se povećala poslednjih 30 godina. Uzrok tome je, verovatno, kombinacija različitih faktora, kao što su ekspanzija i intenzivnije gajenje paprike, povećan obim međunarodne trgovine biljnog materijala čime je povećan rizik unošenja virusa i njihovih vektora u nove regione sveta i lokalitete, kao i klimatske promene koje odgovaraju širenju virusa i njihovih vektora. Do sada je utvrđeno da 68 virusa može da zarazi papriku. Većina virusa paprike je raširena celim svetom, ali ima i izuzetaka, koji su prisutni samo u određenim delovima sveta. Virusi paprike izazivaju ispoljavanje različitih tipova simptoma, a najčešći su mozaik, šarenilo i deformacija lišća koji imaju za posledicu smanjenje porasta biljaka i prinosa. Više od polovine virusa paprike se prenosi biljnim vašima, a drugi česti virusi se prenose leptirastim vašima, tripsima ili mehanički. U Srbiji je utvrđeno prisustvo pet virusa: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV) i virus mozaika duvana (Tobacco mosaic virus, TMV), s tim što su prevalentni CMV i PVY. Neki od tih virusa prouzrokuju značajne gubitke u prinosu. Za svaki ekonomski važan virus u Srbiji navedene su mere kontrole.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Economically important virus disease of pepper in Serbia, Ekonomski značajne viroze paprike u Srbiji",
pages = "627-610",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4544"
}

Krstić, B., Stanković, I., Milošević, D., Vučurović, A.,& Zečević, K.. (2017). Economically important virus disease of pepper in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 610-627.
https://hdl.handle.net/21.15107/rcub_agrospace_4544

Krstić B, Stanković I, Milošević D, Vučurović A, Zečević K. Economically important virus disease of pepper in Serbia. in Biljni lekar. 2017;45(6):610-627.
https://hdl.handle.net/21.15107/rcub_agrospace_4544 .

Krstić, Branka, Stanković, Ivana, Milošević, Dragana, Vučurović, Ana, Zečević, Katarina, "Economically important virus disease of pepper in Serbia" in Biljni lekar, 45, no. 6 (2017):610-627,
https://hdl.handle.net/21.15107/rcub_agrospace_4544 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Krstić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4539
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Presence and distribution of pepper viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 656
IS  - 6
SP  - 647
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4539
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Ignjatov, Maja and Nikolić, Zorica and Krstić, Branka",
year = "2017",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Presence and distribution of pepper viruses in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "656-647",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4539"
}

Milošević, D., Stanković, I., Ignjatov, M., Nikolić, Z.,& Krstić, B.. (2017). Presence and distribution of pepper viruses in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 647-656.
https://hdl.handle.net/21.15107/rcub_agrospace_4539

Milošević D, Stanković I, Ignjatov M, Nikolić Z, Krstić B. Presence and distribution of pepper viruses in Serbia. in Biljni lekar. 2017;45(6):647-656.
https://hdl.handle.net/21.15107/rcub_agrospace_4539 .

Milošević, Dragana, Stanković, Ivana, Ignjatov, Maja, Nikolić, Zorica, Krstić, Branka, "Presence and distribution of pepper viruses in Serbia" in Biljni lekar, 45, no. 6 (2017):647-656,
https://hdl.handle.net/21.15107/rcub_agrospace_4539 .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4542
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4542
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4542"
}

Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.
https://hdl.handle.net/21.15107/rcub_agrospace_4542

Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596.
https://hdl.handle.net/21.15107/rcub_agrospace_4542 .

Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596,
https://hdl.handle.net/21.15107/rcub_agrospace_4542 .

TY  - JOUR
AU  - Jecmenica, Maja
AU  - Kravić, Natalija
AU  - Vasić, Mirjana
AU  - Živanović, Tomislav
AU  - Mandić, Violeta
AU  - Damnjanović, Jelena
AU  - Dragičević, Vesna
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4050
AB  - Characterisation of bean genotypes, particularly local landraces is important for ongoing breeding programs, especially for drought tolerance. Susceptibility to drought is emphasized when bean is grown as a stubble crop and sown at the middle of summer. The aim of this study was to compare variability of ten bean genotypes to optimal (25 degrees C) and higher (30 degrees C) temperatures in combination with optimal (80%) and reduced (40%) field water capacity (FWC), from the point of growth (root and shoot length and fresh matter accumulation) and thermodynamic parameters of free energy (calculated parameter after drying at 60 degrees C, 105 degrees C and 130 degrees C) during the early seedlings stage. Significant and positive correlation between root length and fresh matter with free energy at symplast and chemically bound water occurred under temperature stress (i.e. 30 degrees C). Root growth and elongation were affected by drought stress, i.e. under a combination of high temperature (30 degrees C) and water deficit (40% FWC). Based on higher energy consumption, the ability of shoot to continue a growth in stressful conditions could be possible to achieve, as was confirm through significant and positive correlation between evaluated growth parameters in shoot and free energy of free water. It could be concluded that most of the examined dry bean accessions expressed some sensitivity to stress applied. Among genotypes tested, local landrace zecak expressed lesser susceptibility to stresses applied. Accession Maksa was more tolerant to changes at cytoplasmic level, while Medijana and Sataja 425 expressed root i.e. shoot stress tolerance. Those accessions could be considered as potentially drought tolerant genotypes.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genetic variability of free energy in a function of drought tolerance in common bean accessions
EP  - 1015
IS  - 3
SP  - 1003
VL  - 48
DO  - 10.2298/GENSR1603003J
ER  - 

@article{
author = "Jecmenica, Maja and Kravić, Natalija and Vasić, Mirjana and Živanović, Tomislav and Mandić, Violeta and Damnjanović, Jelena and Dragičević, Vesna",
year = "2016",
abstract = "Characterisation of bean genotypes, particularly local landraces is important for ongoing breeding programs, especially for drought tolerance. Susceptibility to drought is emphasized when bean is grown as a stubble crop and sown at the middle of summer. The aim of this study was to compare variability of ten bean genotypes to optimal (25 degrees C) and higher (30 degrees C) temperatures in combination with optimal (80%) and reduced (40%) field water capacity (FWC), from the point of growth (root and shoot length and fresh matter accumulation) and thermodynamic parameters of free energy (calculated parameter after drying at 60 degrees C, 105 degrees C and 130 degrees C) during the early seedlings stage. Significant and positive correlation between root length and fresh matter with free energy at symplast and chemically bound water occurred under temperature stress (i.e. 30 degrees C). Root growth and elongation were affected by drought stress, i.e. under a combination of high temperature (30 degrees C) and water deficit (40% FWC). Based on higher energy consumption, the ability of shoot to continue a growth in stressful conditions could be possible to achieve, as was confirm through significant and positive correlation between evaluated growth parameters in shoot and free energy of free water. It could be concluded that most of the examined dry bean accessions expressed some sensitivity to stress applied. Among genotypes tested, local landrace zecak expressed lesser susceptibility to stresses applied. Accession Maksa was more tolerant to changes at cytoplasmic level, while Medijana and Sataja 425 expressed root i.e. shoot stress tolerance. Those accessions could be considered as potentially drought tolerant genotypes.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genetic variability of free energy in a function of drought tolerance in common bean accessions",
pages = "1015-1003",
number = "3",
volume = "48",
doi = "10.2298/GENSR1603003J"
}

Jecmenica, M., Kravić, N., Vasić, M., Živanović, T., Mandić, V., Damnjanović, J.,& Dragičević, V.. (2016). Genetic variability of free energy in a function of drought tolerance in common bean accessions. in Genetika
Društvo genetičara Srbije, Beograd., 48(3), 1003-1015.
https://doi.org/10.2298/GENSR1603003J

Jecmenica M, Kravić N, Vasić M, Živanović T, Mandić V, Damnjanović J, Dragičević V. Genetic variability of free energy in a function of drought tolerance in common bean accessions. in Genetika. 2016;48(3):1003-1015.
doi:10.2298/GENSR1603003J .

Jecmenica, Maja, Kravić, Natalija, Vasić, Mirjana, Živanović, Tomislav, Mandić, Violeta, Damnjanović, Jelena, Dragičević, Vesna, "Genetic variability of free energy in a function of drought tolerance in common bean accessions" in Genetika, 48, no. 3 (2016):1003-1015,
https://doi.org/10.2298/GENSR1603003J . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Petrović, Gordana R.
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3684
AB  - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Detection and molecular characterization of pepper mild mottle virus in Serbia
EP  - 663
IS  - 2
SP  - 651
VL  - 47
DO  - 10.2298/GENSR1502651M
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana R. and Krstić, Branka",
year = "2015",
abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Detection and molecular characterization of pepper mild mottle virus in Serbia",
pages = "663-651",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502651M"
}

Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G. R.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 651-663.
https://doi.org/10.2298/GENSR1502651M

Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović GR, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika. 2015;47(2):651-663.
doi:10.2298/GENSR1502651M .

Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana R., Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika, 47, no. 2 (2015):651-663,
https://doi.org/10.2298/GENSR1502651M . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tamindžić, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3947
AB  - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II.
AB  - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
T1  - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
EP  - 73
IS  - 2
SP  - 67
VL  - 52
DO  - 10.5937/ratpov52-7814
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji",
pages = "73-67",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-7814"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73.
https://doi.org/10.5937/ratpov52-7814

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo. 2015;52(2):67-73.
doi:10.5937/ratpov52-7814 .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo, 52, no. 2 (2015):67-73,
https://doi.org/10.5937/ratpov52-7814 . .

TY  - JOUR
AU  - Pejić, Borivoj
AU  - Gajić, Boško
AU  - Bošnjak, Djuro
AU  - Stričević, Ružica
AU  - Mačkić, Ksenija
AU  - Kresović, Branka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3506
AB  - The study of effects of water stress on yield and water use by onion plants was carried out at the experimental field of the Institute of Field and Vegetable Crops in Novi Sad in the period 2005-2007. Onion sensitivity to water stress was determined using a yield response factor (Ky). The values of Ky were derived from the linear relationship between relative evapotranspiration deficits (1-ETa/ETm) and relative yield decrease (1-Ya/Ym). To assess the irrigation effect on onion yield, irrigation water use efficiency (Iwue) and evapotranspiration water use efficiency (ETwue) were determined. Values of Ky in the growing season (Ky 1.78) indicate that onion is highly sensitive to water stress under the climate conditions of the Vojvodina region. The amounts of water used on evapotranspiration under irrigation and non-irrigation conditions ranged from 448.4 to511.9 mm, and 290.2 to 393.9 mm, respectively. The values of Iwue and ETwue varied from 4.35 to 28.05 kg ha-1/m-3 and 7.87 to 19.51 kg ha-1/m-3, respectively, mostly depending on the favorableness of the year for the onion production and irrigation water applied. Ky, Iwue and ETwue can be used as a good basis for onion growers in the region in terms of optimum irrigation water use, for the planning, design and operation of irrigation projects in the region, and also for the improvement the production technology of the crop.
PB  - National Centre for Agrarian Sciences
T2  - Bulgarian Journal of Agricultural Science
T1  - Effects of water stress on water use and yield of onion
EP  - 302
IS  - 2
SP  - 297
VL  - 20
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3506
ER  - 

@article{
author = "Pejić, Borivoj and Gajić, Boško and Bošnjak, Djuro and Stričević, Ružica and Mačkić, Ksenija and Kresović, Branka",
year = "2014",
abstract = "The study of effects of water stress on yield and water use by onion plants was carried out at the experimental field of the Institute of Field and Vegetable Crops in Novi Sad in the period 2005-2007. Onion sensitivity to water stress was determined using a yield response factor (Ky). The values of Ky were derived from the linear relationship between relative evapotranspiration deficits (1-ETa/ETm) and relative yield decrease (1-Ya/Ym). To assess the irrigation effect on onion yield, irrigation water use efficiency (Iwue) and evapotranspiration water use efficiency (ETwue) were determined. Values of Ky in the growing season (Ky 1.78) indicate that onion is highly sensitive to water stress under the climate conditions of the Vojvodina region. The amounts of water used on evapotranspiration under irrigation and non-irrigation conditions ranged from 448.4 to511.9 mm, and 290.2 to 393.9 mm, respectively. The values of Iwue and ETwue varied from 4.35 to 28.05 kg ha-1/m-3 and 7.87 to 19.51 kg ha-1/m-3, respectively, mostly depending on the favorableness of the year for the onion production and irrigation water applied. Ky, Iwue and ETwue can be used as a good basis for onion growers in the region in terms of optimum irrigation water use, for the planning, design and operation of irrigation projects in the region, and also for the improvement the production technology of the crop.",
publisher = "National Centre for Agrarian Sciences",
journal = "Bulgarian Journal of Agricultural Science",
title = "Effects of water stress on water use and yield of onion",
pages = "302-297",
number = "2",
volume = "20",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3506"
}

Pejić, B., Gajić, B., Bošnjak, D., Stričević, R., Mačkić, K.,& Kresović, B.. (2014). Effects of water stress on water use and yield of onion. in Bulgarian Journal of Agricultural Science
National Centre for Agrarian Sciences., 20(2), 297-302.
https://hdl.handle.net/21.15107/rcub_agrospace_3506

Pejić B, Gajić B, Bošnjak D, Stričević R, Mačkić K, Kresović B. Effects of water stress on water use and yield of onion. in Bulgarian Journal of Agricultural Science. 2014;20(2):297-302.
https://hdl.handle.net/21.15107/rcub_agrospace_3506 .

Pejić, Borivoj, Gajić, Boško, Bošnjak, Djuro, Stričević, Ružica, Mačkić, Ksenija, Kresović, Branka, "Effects of water stress on water use and yield of onion" in Bulgarian Journal of Agricultural Science, 20, no. 2 (2014):297-302,
https://hdl.handle.net/21.15107/rcub_agrospace_3506 .

TY  - JOUR
AU  - Gvozdanović-Varga, Jelica
AU  - Vasić, Mirjana
AU  - Červenski, Janko
AU  - Petrović, Anamarija
AU  - Moravčević, Djordje
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3102
AB  - The onion is a widely distributed vegetable crop, which takes an important place in the vegetable production in Serbia. The traditional planting method is the one from sets. Old cultivars and populations and, in recent years, foreign cultivars are grown. The large variety of genotypes, including both domestic populations and cultivars, comprises the significant gene pool of this region. The onion collection of the Institute of Field and Vegetable Crops in Novi Sad is based on the populations and cultivars from the territory of the former Yugoslavia. This paper reviews 30 onion genotypes on the basic IPGRI descriptors (ANNEX I). Variability of the reviewed characteristics was determined by PC analysis. High variability values have been established for bulb skin color, bulb flesh color, bulb hearting and bulb skin thickness. The genotypes varied in bulb skin color as well as in bulb flesh color from white to dark violet. These two characteristics had the largest impact on clustering, with a single genotype being heterogeneous exactly for these two characteristics.
AB  - Crni luk je široko rasprostranjena povrtarska vrsta, čija proizvodnja zauzima značajno mesto u setvenoj strukturi povrća u Srbiji. Tradicionalni način ove proizvodnje je iz arpadžika, gde su zastupljene stare sorte, populacije i sve prisutniji strani sortiment. Bogatstvo genotipova crnog luka, kako gajenih domaćih populacija tako i sorata, predstavlja značajan gen fond sa ovog područja. U Institutu za ratarstvo i povrtarstvo u Novom Sadu, osnovu kolekcije čine domaće populacije i sorte koje se gaje u širem region, sa prostora bivše Jugoslavije. U radu su prikazane osnovne osobine 30 genotipova crnog luka prema deskriptoru IPGRI (ANNEX I.). Varijabilnost ispitivanih osobina je utvrđena PC analizom, gde je ustanovljena visoka varijabilnost za boju lukovice, boju sočnih listova lukovice, broj gnezda i debljinu listova lukovice. Genotipovi su imali boju lukovice od bele do tamno ljubičaste, a takođe i boja sočnih listova. Ove dve osobine su imale najveći uticaj na grupisanje genotipova, tako su formirane dve grupe, uz izdvajanje jednog genotipa koji je heterogen upravo za ove osobine.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection
T1  - Fenotipske razlike u osnovnim karakteristikama kolekcije genotipova crnog luka iz Srbije
EP  - 108
IS  - 1
SP  - 101
VL  - 45
DO  - 10.2298/GENSR1301101G
ER  - 

@article{
author = "Gvozdanović-Varga, Jelica and Vasić, Mirjana and Červenski, Janko and Petrović, Anamarija and Moravčević, Djordje",
year = "2013",
abstract = "The onion is a widely distributed vegetable crop, which takes an important place in the vegetable production in Serbia. The traditional planting method is the one from sets. Old cultivars and populations and, in recent years, foreign cultivars are grown. The large variety of genotypes, including both domestic populations and cultivars, comprises the significant gene pool of this region. The onion collection of the Institute of Field and Vegetable Crops in Novi Sad is based on the populations and cultivars from the territory of the former Yugoslavia. This paper reviews 30 onion genotypes on the basic IPGRI descriptors (ANNEX I). Variability of the reviewed characteristics was determined by PC analysis. High variability values have been established for bulb skin color, bulb flesh color, bulb hearting and bulb skin thickness. The genotypes varied in bulb skin color as well as in bulb flesh color from white to dark violet. These two characteristics had the largest impact on clustering, with a single genotype being heterogeneous exactly for these two characteristics., Crni luk je široko rasprostranjena povrtarska vrsta, čija proizvodnja zauzima značajno mesto u setvenoj strukturi povrća u Srbiji. Tradicionalni način ove proizvodnje je iz arpadžika, gde su zastupljene stare sorte, populacije i sve prisutniji strani sortiment. Bogatstvo genotipova crnog luka, kako gajenih domaćih populacija tako i sorata, predstavlja značajan gen fond sa ovog područja. U Institutu za ratarstvo i povrtarstvo u Novom Sadu, osnovu kolekcije čine domaće populacije i sorte koje se gaje u širem region, sa prostora bivše Jugoslavije. U radu su prikazane osnovne osobine 30 genotipova crnog luka prema deskriptoru IPGRI (ANNEX I.). Varijabilnost ispitivanih osobina je utvrđena PC analizom, gde je ustanovljena visoka varijabilnost za boju lukovice, boju sočnih listova lukovice, broj gnezda i debljinu listova lukovice. Genotipovi su imali boju lukovice od bele do tamno ljubičaste, a takođe i boja sočnih listova. Ove dve osobine su imale najveći uticaj na grupisanje genotipova, tako su formirane dve grupe, uz izdvajanje jednog genotipa koji je heterogen upravo za ove osobine.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection, Fenotipske razlike u osnovnim karakteristikama kolekcije genotipova crnog luka iz Srbije",
pages = "108-101",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301101G"
}

Gvozdanović-Varga, J., Vasić, M., Červenski, J., Petrović, A.,& Moravčević, D.. (2013). Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection. in Genetika
Društvo genetičara Srbije, Beograd., 45(1), 101-108.
https://doi.org/10.2298/GENSR1301101G

Gvozdanović-Varga J, Vasić M, Červenski J, Petrović A, Moravčević D. Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection. in Genetika. 2013;45(1):101-108.
doi:10.2298/GENSR1301101G .

Gvozdanović-Varga, Jelica, Vasić, Mirjana, Červenski, Janko, Petrović, Anamarija, Moravčević, Djordje, "Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection" in Genetika, 45, no. 1 (2013):101-108,
https://doi.org/10.2298/GENSR1301101G . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2937
AB  - Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease.
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Sensitivity of some pepper genotypes to bacterial spot causal agent
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 

@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease., Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Sensitivity of some pepper genotypes to bacterial spot causal agent, Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}

Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769

Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .

Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Sensitivity of some pepper genotypes to bacterial spot causal agent" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Prokić, Andjelka
AU  - Kuzmanović, Nemanja
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2857
AB  - Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri.
AB  - Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Isolation of bacteriophages and their use in Xanthomonas spp. differantiation
T1  - Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.
EP  - 75
IS  - 2
SP  - 62
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2857
ER  - 

@article{
author = "Gašić, Katarina and Ivanović, Milan and Prokić, Andjelka and Kuzmanović, Nemanja and Ignjatov, Maja and Obradović, Aleksa",
year = "2012",
abstract = "Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri., Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation, Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.",
pages = "75-62",
number = "2",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2857"
}

Gašić, K., Ivanović, M., Prokić, A., Kuzmanović, N., Ignjatov, M.,& Obradović, A.. (2012). Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 63(2), 62-75.
https://hdl.handle.net/21.15107/rcub_agrospace_2857

Gašić K, Ivanović M, Prokić A, Kuzmanović N, Ignjatov M, Obradović A. Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja. 2012;63(2):62-75.
https://hdl.handle.net/21.15107/rcub_agrospace_2857 .

Gašić, Katarina, Ivanović, Milan, Prokić, Andjelka, Kuzmanović, Nemanja, Ignjatov, Maja, Obradović, Aleksa, "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation" in Zaštita bilja, 63, no. 2 (2012):62-75,
https://hdl.handle.net/21.15107/rcub_agrospace_2857 .

TY  - JOUR
AU  - Moravčević, Djordje
AU  - Bjelić, Vukašin
AU  - Savić, Dubravka
AU  - Gvozdanović-Varga, Jelica
AU  - Beatović, Damir
AU  - Jelačić, Slavica
AU  - Zarić, Vlade
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2598
AB  - Field experiments were conducted to study green garlic response to the following plant densities: 300,000; 450,000; 600,000; 750,000 and 900,000 plants/ha. The experiment lasted for two years. Plant measurements started 40 days after planting (DAP) and were performed at ten-day intervals. Eight measurements per year were made in total and the following traits were measured: leaf number per plant, leaf surface area and leaf area index (LAI). These parameters were used to determine the effects of stand density on the photosynthetic apparatus of the garlic plant. These effects were found to be present, but to varying degrees. Stand density had the strongest effect on LAI and the smallest on leaf area. The data collected during the experiment and the appearance of the garlic plants themselves showed that the crop performed best at moderate stand densities (600,000 and 750,000 plants/ha). At such densities, garlic developed a powerful photosynthetic apparatus and the bulbs were large and uniform.
PB  - Academic Journals, Victoria Island
T2  - African Journal of Biotechnology
T1  - Effect of plant density on the characteristics of photosynthetic apparatus of garlic (Allium sativum var. vulgare L.)
EP  - 15868
IS  - 71
SP  - 15861
VL  - 10
DO  - 10.5897/AJB11.105
ER  - 

@article{
author = "Moravčević, Djordje and Bjelić, Vukašin and Savić, Dubravka and Gvozdanović-Varga, Jelica and Beatović, Damir and Jelačić, Slavica and Zarić, Vlade",
year = "2011",
abstract = "Field experiments were conducted to study green garlic response to the following plant densities: 300,000; 450,000; 600,000; 750,000 and 900,000 plants/ha. The experiment lasted for two years. Plant measurements started 40 days after planting (DAP) and were performed at ten-day intervals. Eight measurements per year were made in total and the following traits were measured: leaf number per plant, leaf surface area and leaf area index (LAI). These parameters were used to determine the effects of stand density on the photosynthetic apparatus of the garlic plant. These effects were found to be present, but to varying degrees. Stand density had the strongest effect on LAI and the smallest on leaf area. The data collected during the experiment and the appearance of the garlic plants themselves showed that the crop performed best at moderate stand densities (600,000 and 750,000 plants/ha). At such densities, garlic developed a powerful photosynthetic apparatus and the bulbs were large and uniform.",
publisher = "Academic Journals, Victoria Island",
journal = "African Journal of Biotechnology",
title = "Effect of plant density on the characteristics of photosynthetic apparatus of garlic (Allium sativum var. vulgare L.)",
pages = "15868-15861",
number = "71",
volume = "10",
doi = "10.5897/AJB11.105"
}

Moravčević, D., Bjelić, V., Savić, D., Gvozdanović-Varga, J., Beatović, D., Jelačić, S.,& Zarić, V.. (2011). Effect of plant density on the characteristics of photosynthetic apparatus of garlic (Allium sativum var. vulgare L.). in African Journal of Biotechnology
Academic Journals, Victoria Island., 10(71), 15861-15868.
https://doi.org/10.5897/AJB11.105

Moravčević D, Bjelić V, Savić D, Gvozdanović-Varga J, Beatović D, Jelačić S, Zarić V. Effect of plant density on the characteristics of photosynthetic apparatus of garlic (Allium sativum var. vulgare L.). in African Journal of Biotechnology. 2011;10(71):15861-15868.
doi:10.5897/AJB11.105 .

Moravčević, Djordje, Bjelić, Vukašin, Savić, Dubravka, Gvozdanović-Varga, Jelica, Beatović, Damir, Jelačić, Slavica, Zarić, Vlade, "Effect of plant density on the characteristics of photosynthetic apparatus of garlic (Allium sativum var. vulgare L.)" in African Journal of Biotechnology, 10, no. 71 (2011):15861-15868,
https://doi.org/10.5897/AJB11.105 . .