TY  - JOUR
AU  - Ismail, Emadeldeen
AU  - Blom, Jochen
AU  - Bultreys, Alain
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
AU  - van Doorn, Joop
AU  - Bergsma-Vlami, Maria
AU  - Maes, Martine
AU  - Willems, Anne
AU  - Duffy, Brion
AU  - Stockwell, Virginia O.
AU  - Smits, Theo H.M.
AU  - Pulawska, Joanna
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3470
AB  - Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.
PB  - Springer, New York
T2  - Archives of Microbiology
T1  - A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids
EP  - 899
IS  - 12
SP  - 891
VL  - 196
DO  - 10.1007/s00203-014-1028-5
ER  - 

@article{
author = "Ismail, Emadeldeen and Blom, Jochen and Bultreys, Alain and Ivanović, Milan and Obradović, Aleksa and van Doorn, Joop and Bergsma-Vlami, Maria and Maes, Martine and Willems, Anne and Duffy, Brion and Stockwell, Virginia O. and Smits, Theo H.M. and Pulawska, Joanna",
year = "2014",
abstract = "Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.",
publisher = "Springer, New York",
journal = "Archives of Microbiology",
title = "A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids",
pages = "899-891",
number = "12",
volume = "196",
doi = "10.1007/s00203-014-1028-5"
}

Ismail, E., Blom, J., Bultreys, A., Ivanović, M., Obradović, A., van Doorn, J., Bergsma-Vlami, M., Maes, M., Willems, A., Duffy, B., Stockwell, V. O., Smits, T. H.M.,& Pulawska, J.. (2014). A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids. in Archives of Microbiology
Springer, New York., 196(12), 891-899.
https://doi.org/10.1007/s00203-014-1028-5

Ismail E, Blom J, Bultreys A, Ivanović M, Obradović A, van Doorn J, Bergsma-Vlami M, Maes M, Willems A, Duffy B, Stockwell VO, Smits TH, Pulawska J. A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids. in Archives of Microbiology. 2014;196(12):891-899.
doi:10.1007/s00203-014-1028-5 .

Ismail, Emadeldeen, Blom, Jochen, Bultreys, Alain, Ivanović, Milan, Obradović, Aleksa, van Doorn, Joop, Bergsma-Vlami, Maria, Maes, Martine, Willems, Anne, Duffy, Brion, Stockwell, Virginia O., Smits, Theo H.M., Pulawska, Joanna, "A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids" in Archives of Microbiology, 196, no. 12 (2014):891-899,
https://doi.org/10.1007/s00203-014-1028-5 . .