Increasing the market significance of forage crops by breeding and optimizing seed production technology

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Increasing the market significance of forage crops by breeding and optimizing seed production technology (en)
Повећање тржишног значаја крмних биљака оплемењивањем и оптимизацијом технологије производње семена (sr)
Povećanje tržišnog značaja krmnih biljaka oplemenjivanjem i optimizacijom tehnologije proizvodnje semena (sr_RS)
Authors

Publications

Variability of red clover genotypes on the basis of morphological markers

Radinović, Irena; Vasiljević, Sanja; Zorić, Miroslav; Branković, Gordana; Živanović, Tomislav; Prodanović, Slaven

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Zorić, Miroslav
AU  - Branković, Gordana
AU  - Živanović, Tomislav
AU  - Prodanović, Slaven
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4670
AB  - Red clover (Trifolium pratense L.) is an important forage legume of temperate regions dominantly used as a source of animal food. The present research aimed at assessment of genetic diversity based on morphological markers, through the analyses of five morphological markers in a collection of 46 red clover genotypes. These morphological markers were screened according to the UPOV descriptor (2001) in the trial laid out in a randomized complete block design with three replications. The traits analyzed and investigated were: time of flowering, growth habit, density of hairs, leaf color and intensity of white marks. The average value of Shannon's diversity index for five morphological markers amounted 0.711. Homogeneity analysis (HOMALS) of the same five descriptors accounted for 71.2% of the total variation of the standardized data, with the first and second axis explaining 38.4% and 32.8% of the morphological variability, respectively. Based on this analyses all red clover genotypes were grouped into seven homogeneous groups in two-dimensional space, thus providing visualization of genotypes diversity based on their morphological traits. UPGMA cluster analysis of the same morphological markers allowed the description of four groups with genetic distances represented by the simple matching coefficient of similarity ranging from 0.00 to 1.00. Observed results achieved by these two analyses were compared and although both of analyses were successful in grouping and discrimination of red clover genotypes with some similarities and differences, priority in future breeding programs was given to the HOMALS.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Variability of red clover genotypes on the basis of morphological markers
EP  - 906
IS  - 3
SP  - 895
VL  - 50
DO  - 10.2298/GENSR1803895R
ER  - 
@article{
author = "Radinović, Irena and Vasiljević, Sanja and Zorić, Miroslav and Branković, Gordana and Živanović, Tomislav and Prodanović, Slaven",
year = "2018",
abstract = "Red clover (Trifolium pratense L.) is an important forage legume of temperate regions dominantly used as a source of animal food. The present research aimed at assessment of genetic diversity based on morphological markers, through the analyses of five morphological markers in a collection of 46 red clover genotypes. These morphological markers were screened according to the UPOV descriptor (2001) in the trial laid out in a randomized complete block design with three replications. The traits analyzed and investigated were: time of flowering, growth habit, density of hairs, leaf color and intensity of white marks. The average value of Shannon's diversity index for five morphological markers amounted 0.711. Homogeneity analysis (HOMALS) of the same five descriptors accounted for 71.2% of the total variation of the standardized data, with the first and second axis explaining 38.4% and 32.8% of the morphological variability, respectively. Based on this analyses all red clover genotypes were grouped into seven homogeneous groups in two-dimensional space, thus providing visualization of genotypes diversity based on their morphological traits. UPGMA cluster analysis of the same morphological markers allowed the description of four groups with genetic distances represented by the simple matching coefficient of similarity ranging from 0.00 to 1.00. Observed results achieved by these two analyses were compared and although both of analyses were successful in grouping and discrimination of red clover genotypes with some similarities and differences, priority in future breeding programs was given to the HOMALS.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Variability of red clover genotypes on the basis of morphological markers",
pages = "906-895",
number = "3",
volume = "50",
doi = "10.2298/GENSR1803895R"
}
Radinović, I., Vasiljević, S., Zorić, M., Branković, G., Živanović, T.,& Prodanović, S.. (2018). Variability of red clover genotypes on the basis of morphological markers. in Genetika
Društvo genetičara Srbije, Beograd., 50(3), 895-906.
https://doi.org/10.2298/GENSR1803895R
Radinović I, Vasiljević S, Zorić M, Branković G, Živanović T, Prodanović S. Variability of red clover genotypes on the basis of morphological markers. in Genetika. 2018;50(3):895-906.
doi:10.2298/GENSR1803895R .
Radinović, Irena, Vasiljević, Sanja, Zorić, Miroslav, Branković, Gordana, Živanović, Tomislav, Prodanović, Slaven, "Variability of red clover genotypes on the basis of morphological markers" in Genetika, 50, no. 3 (2018):895-906,
https://doi.org/10.2298/GENSR1803895R . .
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Assessment of some parameters productivity and quality of populations phleum pratense l. Grown in conditions of Serbia

Janković, Vesna; Vučković, Savo; Mihailović, Vojislav; Popović, Vera; Živanović, Ljubiša; Simić, Divna; Vujošević, Ana; Stevanović, Petar

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Janković, Vesna
AU  - Vučković, Savo
AU  - Mihailović, Vojislav
AU  - Popović, Vera
AU  - Živanović, Ljubiša
AU  - Simić, Divna
AU  - Vujošević, Ana
AU  - Stevanović, Petar
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4767
AB  - Phleum pratense (L.) is the important forage grass species in Serbia. The study was conducted on 20 autochthonous population of Phleum pratense (L.), originating from Western Serbia. Plant height, protein content and crude cellulose content had a high direct impact on yield. Populations of PP16, PP20, PP2 and PP9 had the significantly higher yield of the raw matter compared to all the tested population and the highest values for the other evaluated parameters, also. The average yield of crude biomass was positive statistically highly significantly correlated with plant height (r=0.87**), and positively significantly correlated with a content of crude proteins and positive non significant correlated with crude cellulose (r=0.42 ). Based on obtained values and by the appropriate choice of selection methods, we conclude that we have excellent genotypes, PP16, PP20, PP2 and PP9 for a successful selection process in order to obtain new high yielding varieties of Phleum pratense.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Assessment of some parameters productivity and quality of populations phleum pratense l. Grown in conditions of Serbia
EP  - 10
IS  - 1
SP  - 1
VL  - 50
DO  - 10.2298/GENSR1801001J
ER  - 
@article{
author = "Janković, Vesna and Vučković, Savo and Mihailović, Vojislav and Popović, Vera and Živanović, Ljubiša and Simić, Divna and Vujošević, Ana and Stevanović, Petar",
year = "2018",
abstract = "Phleum pratense (L.) is the important forage grass species in Serbia. The study was conducted on 20 autochthonous population of Phleum pratense (L.), originating from Western Serbia. Plant height, protein content and crude cellulose content had a high direct impact on yield. Populations of PP16, PP20, PP2 and PP9 had the significantly higher yield of the raw matter compared to all the tested population and the highest values for the other evaluated parameters, also. The average yield of crude biomass was positive statistically highly significantly correlated with plant height (r=0.87**), and positively significantly correlated with a content of crude proteins and positive non significant correlated with crude cellulose (r=0.42 ). Based on obtained values and by the appropriate choice of selection methods, we conclude that we have excellent genotypes, PP16, PP20, PP2 and PP9 for a successful selection process in order to obtain new high yielding varieties of Phleum pratense.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Assessment of some parameters productivity and quality of populations phleum pratense l. Grown in conditions of Serbia",
pages = "10-1",
number = "1",
volume = "50",
doi = "10.2298/GENSR1801001J"
}
Janković, V., Vučković, S., Mihailović, V., Popović, V., Živanović, L., Simić, D., Vujošević, A.,& Stevanović, P.. (2018). Assessment of some parameters productivity and quality of populations phleum pratense l. Grown in conditions of Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 50(1), 1-10.
https://doi.org/10.2298/GENSR1801001J
Janković V, Vučković S, Mihailović V, Popović V, Živanović L, Simić D, Vujošević A, Stevanović P. Assessment of some parameters productivity and quality of populations phleum pratense l. Grown in conditions of Serbia. in Genetika. 2018;50(1):1-10.
doi:10.2298/GENSR1801001J .
Janković, Vesna, Vučković, Savo, Mihailović, Vojislav, Popović, Vera, Živanović, Ljubiša, Simić, Divna, Vujošević, Ana, Stevanović, Petar, "Assessment of some parameters productivity and quality of populations phleum pratense l. Grown in conditions of Serbia" in Genetika, 50, no. 1 (2018):1-10,
https://doi.org/10.2298/GENSR1801001J . .
2
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Variability of red clover genotypes on the basis of agronomic traits, morphological and microsatellite markers

Radinović, Irena

(Univerzitet u Beogradu, Poljoprivredni fakultet, 2017)

TY  - THES
AU  - Radinović, Irena
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/49
AB  - Red clover (Trifolium pratense L.) is cross-pollinated plant species which is characterized by gametophytic self-incompatibility, and its populations are heterogeneous with heterozygous genotypes. The prior characterization and evaluation of samples that are in the collections of germplasm are important for using plant genetic resources in breeding programs. A thorough and comprehensive description of red clover germplasm is one of the main prerequisites for achieving a successful breeding. The aim of this doctoral thesis was to evaluate the phenotype variability of 46 genotypes of red clover as part of the collection of the Institute of Field and Vegetable Crops in Novi Sad, on the basis of agronomically important traits and morphological markers by UPOV. Molecular characterization of 46 genotypes of red clover was performed by applying the set of 15 microsatellite markers in order to establish the genetic variability of selected materials and its importance in breeding for certain agronomically important traits. In addition, in the tested material were determined certain important quality parameters: crude protein (CP) content, acid detergent fiber (ADF) and neutral detergent fiber (NDF). Through the analysis of four morphological traits (growth habit, density of hairs, leaf color and intensity of white marks) and one phenological trait) it was determined a high level of diversity of red clover genotypes and time of flowering is defined as the descriptor of the greatest discriminatory power. There was some degree of consensus in grouping genotypes using the analysis of homogeneity in relation to cluster analysis. In determining the morphological similarities and determining the qualities that contribute most to the discrimination of genotypes of red clover, homogeneity analysis has proven to be better compared to the cluster method...
AB  - Crvena detelina (Trifolium pratense L.) je izrazito stranooplodna biljna vrsta koja se odlikuje gametofitskim sistemom inkompatibilnosti, a populacije su heterogene i sastoje se od heterozigotnih genotipova. Da bi biljni genetički resursi mogli biti korišćeni u programima oplemenjivanja, neophodno je izvršiti prethodnu karakterizaciju i evaluaciju uzoraka, koji se nalaze u kolekcijama germplazme. Precizan i sveobuhvatan opis germplazme crvene deteline je jedan od osnovnih preduslova u ostvarivanju uspešnog oplemenjivanja. Cilj ove doktorske disertacije je obuhvatao evaluaciju fenotipske varijabilnosti 46 genotipova crvene deteline koji su deo kolekcije Instituta za ratarstvo i povrtarstvo u Novom Sadu, a na osnovu agronomski značajnih osobina i morfoloških markera po UPOV-u. Primenom odabranog seta od 15 mikrosatelitskih markera izvršena je molekularna karakterizacija 46 genotipova crvene deteline, kako bi se ustanovila genetička varijabilnost materijala i njegov značaj za oplemenjivanje na određene agronomski važne osobine. Osim toga, kod ispitivanih genotipova crvene deteline su određeni i značajni parametri kvaliteta: sadržaj sirovih proteina, vlakna nerastvorljiva u kiselom deterdžentu (ADF-acid detergent fiber) i vlakna nerastvorljiva u neutralnom deterdžentu (NDF- neutral detergent fiber). Na osnovu analize četiri morfološke osobine (forma rasta, maljavost stabljike, boja lista i intenzitet obojenosti pege na listu) i jedne fenološke osobine (vreme cvetanja), utvrđen je visok nivo diverziteta ispitivanih genotipova crvene deteline, a vreme cvetanja je određeno kao deskriptor najveće diskriminacione moći. Postignut je određeni stepen saglasnosti u grupisanju genotipova primenom analize homogenosti u odnosu na klaster analizu. Pri utvrđivanju morfološke sličnosti i određivanju osobina koje najviše doprinose diskriminaciji genotipova crvene deteline metod analize homogenosti se pokazao kao bolji u odnosu na klaster metod...
PB  - Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Variability of red clover genotypes on the basis of agronomic traits, morphological and microsatellite markers
T1  - Varijabilnost genotipova crvene deteline na osnovu agronomskih osobina, morfoloških i mikrosatelitskih markera
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8109
ER  - 
@phdthesis{
author = "Radinović, Irena",
year = "2017",
abstract = "Red clover (Trifolium pratense L.) is cross-pollinated plant species which is characterized by gametophytic self-incompatibility, and its populations are heterogeneous with heterozygous genotypes. The prior characterization and evaluation of samples that are in the collections of germplasm are important for using plant genetic resources in breeding programs. A thorough and comprehensive description of red clover germplasm is one of the main prerequisites for achieving a successful breeding. The aim of this doctoral thesis was to evaluate the phenotype variability of 46 genotypes of red clover as part of the collection of the Institute of Field and Vegetable Crops in Novi Sad, on the basis of agronomically important traits and morphological markers by UPOV. Molecular characterization of 46 genotypes of red clover was performed by applying the set of 15 microsatellite markers in order to establish the genetic variability of selected materials and its importance in breeding for certain agronomically important traits. In addition, in the tested material were determined certain important quality parameters: crude protein (CP) content, acid detergent fiber (ADF) and neutral detergent fiber (NDF). Through the analysis of four morphological traits (growth habit, density of hairs, leaf color and intensity of white marks) and one phenological trait) it was determined a high level of diversity of red clover genotypes and time of flowering is defined as the descriptor of the greatest discriminatory power. There was some degree of consensus in grouping genotypes using the analysis of homogeneity in relation to cluster analysis. In determining the morphological similarities and determining the qualities that contribute most to the discrimination of genotypes of red clover, homogeneity analysis has proven to be better compared to the cluster method..., Crvena detelina (Trifolium pratense L.) je izrazito stranooplodna biljna vrsta koja se odlikuje gametofitskim sistemom inkompatibilnosti, a populacije su heterogene i sastoje se od heterozigotnih genotipova. Da bi biljni genetički resursi mogli biti korišćeni u programima oplemenjivanja, neophodno je izvršiti prethodnu karakterizaciju i evaluaciju uzoraka, koji se nalaze u kolekcijama germplazme. Precizan i sveobuhvatan opis germplazme crvene deteline je jedan od osnovnih preduslova u ostvarivanju uspešnog oplemenjivanja. Cilj ove doktorske disertacije je obuhvatao evaluaciju fenotipske varijabilnosti 46 genotipova crvene deteline koji su deo kolekcije Instituta za ratarstvo i povrtarstvo u Novom Sadu, a na osnovu agronomski značajnih osobina i morfoloških markera po UPOV-u. Primenom odabranog seta od 15 mikrosatelitskih markera izvršena je molekularna karakterizacija 46 genotipova crvene deteline, kako bi se ustanovila genetička varijabilnost materijala i njegov značaj za oplemenjivanje na određene agronomski važne osobine. Osim toga, kod ispitivanih genotipova crvene deteline su određeni i značajni parametri kvaliteta: sadržaj sirovih proteina, vlakna nerastvorljiva u kiselom deterdžentu (ADF-acid detergent fiber) i vlakna nerastvorljiva u neutralnom deterdžentu (NDF- neutral detergent fiber). Na osnovu analize četiri morfološke osobine (forma rasta, maljavost stabljike, boja lista i intenzitet obojenosti pege na listu) i jedne fenološke osobine (vreme cvetanja), utvrđen je visok nivo diverziteta ispitivanih genotipova crvene deteline, a vreme cvetanja je određeno kao deskriptor najveće diskriminacione moći. Postignut je određeni stepen saglasnosti u grupisanju genotipova primenom analize homogenosti u odnosu na klaster analizu. Pri utvrđivanju morfološke sličnosti i određivanju osobina koje najviše doprinose diskriminaciji genotipova crvene deteline metod analize homogenosti se pokazao kao bolji u odnosu na klaster metod...",
publisher = "Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Variability of red clover genotypes on the basis of agronomic traits, morphological and microsatellite markers, Varijabilnost genotipova crvene deteline na osnovu agronomskih osobina, morfoloških i mikrosatelitskih markera",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8109"
}
Radinović, I.. (2017). Variability of red clover genotypes on the basis of agronomic traits, morphological and microsatellite markers. 
Univerzitet u Beogradu, Poljoprivredni fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_8109
Radinović I. Variability of red clover genotypes on the basis of agronomic traits, morphological and microsatellite markers. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8109 .
Radinović, Irena, "Variability of red clover genotypes on the basis of agronomic traits, morphological and microsatellite markers" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8109 .

Molecular characterization of red clover genotypes utilizing microsatellite markers

Radinović, Irena; Vasiljević, Sanja; Branković, Gordana; Ahsyee, Ramadan Salem; Momirović, Una; Perović, Dragan; Šurlan-Momirović, Gordana

(Inst Investigaciones Agropecuarias, Chillan, 2017)

TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Branković, Gordana
AU  - Ahsyee, Ramadan Salem
AU  - Momirović, Una
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4331
AB  - Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Molecular characterization of red clover genotypes utilizing microsatellite markers
EP  - 47
IS  - 1
SP  - 41
VL  - 77
DO  - 10.4067/S0718-58392017000100005
ER  - 
@article{
author = "Radinović, Irena and Vasiljević, Sanja and Branković, Gordana and Ahsyee, Ramadan Salem and Momirović, Una and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2017",
abstract = "Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Molecular characterization of red clover genotypes utilizing microsatellite markers",
pages = "47-41",
number = "1",
volume = "77",
doi = "10.4067/S0718-58392017000100005"
}
Radinović, I., Vasiljević, S., Branković, G., Ahsyee, R. S., Momirović, U., Perović, D.,& Šurlan-Momirović, G.. (2017). Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 77(1), 41-47.
https://doi.org/10.4067/S0718-58392017000100005
Radinović I, Vasiljević S, Branković G, Ahsyee RS, Momirović U, Perović D, Šurlan-Momirović G. Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research. 2017;77(1):41-47.
doi:10.4067/S0718-58392017000100005 .
Radinović, Irena, Vasiljević, Sanja, Branković, Gordana, Ahsyee, Ramadan Salem, Momirović, Una, Perović, Dragan, Šurlan-Momirović, Gordana, "Molecular characterization of red clover genotypes utilizing microsatellite markers" in Chilean Journal of Agricultural Research, 77, no. 1 (2017):41-47,
https://doi.org/10.4067/S0718-58392017000100005 . .
9
3
10

Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers

Ahsyee, Salem R.; Al-Sloge, O.; Ćalić, Irena; Branković, Gordana; Zorić, M.; Momirović, Una; Vasiljević, Sanja; Šurlan-Momirović, Gordana

(Srpsko biološko društvo, Beograd, i dr., 2013)

TY  - JOUR
AU  - Ahsyee, Salem R.
AU  - Al-Sloge, O.
AU  - Ćalić, Irena
AU  - Branković, Gordana
AU  - Zorić, M.
AU  - Momirović, Una
AU  - Vasiljević, Sanja
AU  - Šurlan-Momirović, Gordana
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3169
AB  - Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers
EP  - 602
IS  - 2
SP  - 595
VL  - 65
DO  - 10.2298/ABS1302595A
ER  - 
@article{
author = "Ahsyee, Salem R. and Al-Sloge, O. and Ćalić, Irena and Branković, Gordana and Zorić, M. and Momirović, Una and Vasiljević, Sanja and Šurlan-Momirović, Gordana",
year = "2013",
abstract = "Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers",
pages = "602-595",
number = "2",
volume = "65",
doi = "10.2298/ABS1302595A"
}
Ahsyee, S. R., Al-Sloge, O., Ćalić, I., Branković, G., Zorić, M., Momirović, U., Vasiljević, S.,& Šurlan-Momirović, G.. (2013). Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(2), 595-602.
https://doi.org/10.2298/ABS1302595A
Ahsyee SR, Al-Sloge O, Ćalić I, Branković G, Zorić M, Momirović U, Vasiljević S, Šurlan-Momirović G. Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences. 2013;65(2):595-602.
doi:10.2298/ABS1302595A .
Ahsyee, Salem R., Al-Sloge, O., Ćalić, Irena, Branković, Gordana, Zorić, M., Momirović, Una, Vasiljević, Sanja, Šurlan-Momirović, Gordana, "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers" in Archives of Biological Sciences, 65, no. 2 (2013):595-602,
https://doi.org/10.2298/ABS1302595A . .
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