TY  - JOUR
AU  - Dong, Liwei
AU  - Li, Ning
AU  - Lu, Xiaobing
AU  - Prodanović, Slaven
AU  - Xu, Yanhao
AU  - Zhang, Wenying
AU  - Yan, Yueming
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4191
AB  - Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines
EP  - 269
IS  - 1
SP  - 249
VL  - 48
DO  - 10.2298/GENSR1601249D
ER  - 

@article{
author = "Dong, Liwei and Li, Ning and Lu, Xiaobing and Prodanović, Slaven and Xu, Yanhao and Zhang, Wenying and Yan, Yueming",
year = "2016",
abstract = "Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines",
pages = "269-249",
number = "1",
volume = "48",
doi = "10.2298/GENSR1601249D"
}

Dong, L., Li, N., Lu, X., Prodanović, S., Xu, Y., Zhang, W.,& Yan, Y.. (2016). Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 249-269.
https://doi.org/10.2298/GENSR1601249D

Dong L, Li N, Lu X, Prodanović S, Xu Y, Zhang W, Yan Y. Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika. 2016;48(1):249-269.
doi:10.2298/GENSR1601249D .

Dong, Liwei, Li, Ning, Lu, Xiaobing, Prodanović, Slaven, Xu, Yanhao, Zhang, Wenying, Yan, Yueming, "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines" in Genetika, 48, no. 1 (2016):249-269,
https://doi.org/10.2298/GENSR1601249D . .

TY  - JOUR
AU  - Zhu, Gengrui
AU  - Wang, Shunli
AU  - Zhen, Shoumin
AU  - Shen, Xixi
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3839
AB  - Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima
EP  - 203
IS  - 1
SP  - 185
VL  - 47
DO  - 10.2298/GENSR1501185Z
ER  - 

@article{
author = "Zhu, Gengrui and Wang, Shunli and Zhen, Shoumin and Shen, Xixi and Prodanović, Slaven and Yan, Yueming",
year = "2015",
abstract = "Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima",
pages = "203-185",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501185Z"
}

Zhu, G., Wang, S., Zhen, S., Shen, X., Prodanović, S.,& Yan, Y.. (2015). Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 185-203.
https://doi.org/10.2298/GENSR1501185Z

Zhu G, Wang S, Zhen S, Shen X, Prodanović S, Yan Y. Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika. 2015;47(1):185-203.
doi:10.2298/GENSR1501185Z .

Zhu, Gengrui, Wang, Shunli, Zhen, Shoumin, Shen, Xixi, Prodanović, Slaven, Yan, Yueming, "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima" in Genetika, 47, no. 1 (2015):185-203,
https://doi.org/10.2298/GENSR1501185Z . .