TY  - JOUR
AU  - Wang, S.L.
AU  - Chen, D.
AU  - Guo, G.F.
AU  - Zhang, T.
AU  - Jiang, S.S.
AU  - Shen, X.X.
AU  - Perović, D.
AU  - Prodanović, Slaven
AU  - Yan, Y.M.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3038
AB  - In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Cereal Research Communications
T1  - Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species
EP  - 551
IS  - 4
SP  - 542
VL  - 40
DO  - 10.1556/CRC.40.2012.0006
ER  - 

@article{
author = "Wang, S.L. and Chen, D. and Guo, G.F. and Zhang, T. and Jiang, S.S. and Shen, X.X. and Perović, D. and Prodanović, Slaven and Yan, Y.M.",
year = "2012",
abstract = "In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Cereal Research Communications",
title = "Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species",
pages = "551-542",
number = "4",
volume = "40",
doi = "10.1556/CRC.40.2012.0006"
}

Wang, S.L., Chen, D., Guo, G.F., Zhang, T., Jiang, S.S., Shen, X.X., Perović, D., Prodanović, S.,& Yan, Y.M.. (2012). Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species. in Cereal Research Communications
Akademiai Kiado Zrt, Budapest., 40(4), 542-551.
https://doi.org/10.1556/CRC.40.2012.0006

Wang S, Chen D, Guo G, Zhang T, Jiang S, Shen X, Perović D, Prodanović S, Yan Y. Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species. in Cereal Research Communications. 2012;40(4):542-551.
doi:10.1556/CRC.40.2012.0006 .

Wang, S.L., Chen, D., Guo, G.F., Zhang, T., Jiang, S.S., Shen, X.X., Perović, D., Prodanović, Slaven, Yan, Y.M., "Molecular Characterization of LMW-GS Genes from C, N, U and S-s Genomes among Aegilops Species" in Cereal Research Communications, 40, no. 4 (2012):542-551,
https://doi.org/10.1556/CRC.40.2012.0006 . .