TY  - JOUR
AU  - Zhu, Gengrui
AU  - Wang, Shunli
AU  - Zhen, Shoumin
AU  - Shen, Xixi
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3839
AB  - Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima
EP  - 203
IS  - 1
SP  - 185
VL  - 47
DO  - 10.2298/GENSR1501185Z
ER  - 

@article{
author = "Zhu, Gengrui and Wang, Shunli and Zhen, Shoumin and Shen, Xixi and Prodanović, Slaven and Yan, Yueming",
year = "2015",
abstract = "Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima",
pages = "203-185",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501185Z"
}

Zhu, G., Wang, S., Zhen, S., Shen, X., Prodanović, S.,& Yan, Y.. (2015). Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 185-203.
https://doi.org/10.2298/GENSR1501185Z

Zhu G, Wang S, Zhen S, Shen X, Prodanović S, Yan Y. Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika. 2015;47(1):185-203.
doi:10.2298/GENSR1501185Z .

Zhu, Gengrui, Wang, Shunli, Zhen, Shoumin, Shen, Xixi, Prodanović, Slaven, Yan, Yueming, "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima" in Genetika, 47, no. 1 (2015):185-203,
https://doi.org/10.2298/GENSR1501185Z . .