TY  - JOUR
AU  - Oketić, K.
AU  - Bogović-Matijasić, B.
AU  - Obermajer, T.
AU  - Radulović, Zorica
AU  - Lević, Steva
AU  - Mirković, Nemanja
AU  - Nedović, Viktor
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3763
AB  - The aim of the study was to evaluate real-time PCR coupled with propidium monoazide (PMA) treatment for enumeration of microencapsulated probiotic lactobacilli microencapsulated in calcium alginate beads. Lactobacillus gasseri K7 (CCM 7710) and Lactobacillus delbrueckii subsp. bulgaricus (CCM 7712) were analysed by plate counting and PMA real-time PCR during storage at 4 degrees C for 90 days. PMA was effective in preventing PCR amplification of the target sequences of DNA released from heat-compromised bacteria. The values obtained by real-time PCR of non-treated samples were in general higher than those obtained by real-time PCR of PMA-treated samples or by plate counting, indicating the presence of sub-lethally injured cells. This study shows that plate count could not be completely replaced by culture independent method PMA real-time PCR for enumeration of probiotics, but may rather complement the well-established plate counting, providing useful information about the ratio of compromised bacteria in the samples.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads
EP  - 581
IS  - 4
SP  - 573
VL  - 6
DO  - 10.3920/BM2014.0095
ER  - 

@article{
author = "Oketić, K. and Bogović-Matijasić, B. and Obermajer, T. and Radulović, Zorica and Lević, Steva and Mirković, Nemanja and Nedović, Viktor",
year = "2015",
abstract = "The aim of the study was to evaluate real-time PCR coupled with propidium monoazide (PMA) treatment for enumeration of microencapsulated probiotic lactobacilli microencapsulated in calcium alginate beads. Lactobacillus gasseri K7 (CCM 7710) and Lactobacillus delbrueckii subsp. bulgaricus (CCM 7712) were analysed by plate counting and PMA real-time PCR during storage at 4 degrees C for 90 days. PMA was effective in preventing PCR amplification of the target sequences of DNA released from heat-compromised bacteria. The values obtained by real-time PCR of non-treated samples were in general higher than those obtained by real-time PCR of PMA-treated samples or by plate counting, indicating the presence of sub-lethally injured cells. This study shows that plate count could not be completely replaced by culture independent method PMA real-time PCR for enumeration of probiotics, but may rather complement the well-established plate counting, providing useful information about the ratio of compromised bacteria in the samples.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads",
pages = "581-573",
number = "4",
volume = "6",
doi = "10.3920/BM2014.0095"
}

Oketić, K., Bogović-Matijasić, B., Obermajer, T., Radulović, Z., Lević, S., Mirković, N.,& Nedović, V.. (2015). Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 6(4), 573-581.
https://doi.org/10.3920/BM2014.0095

Oketić K, Bogović-Matijasić B, Obermajer T, Radulović Z, Lević S, Mirković N, Nedović V. Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads. in Beneficial Microbes. 2015;6(4):573-581.
doi:10.3920/BM2014.0095 .

Oketić, K., Bogović-Matijasić, B., Obermajer, T., Radulović, Zorica, Lević, Steva, Mirković, Nemanja, Nedović, Viktor, "Evaluation of propidium monoazide real-time PCR for enumeration of probiotic lactobacilli microencapsulated in calcium alginate beads" in Beneficial Microbes, 6, no. 4 (2015):573-581,
https://doi.org/10.3920/BM2014.0095 . .