TY  - JOUR
AU  - Machado-Moreira, Bernardino
AU  - Monteiro, Silvia
AU  - Santos, Ricardo
AU  - Martinez-Murcia, Antonio
AU  - Rajković, Andreja
AU  - Šmigić, Nada
AU  - Richards, Karl G.
AU  - Abram, Florence
AU  - Burgess, Catherine M.
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5356
AB  - There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.
PB  - Elsevier, Amsterdam
T2  - Journal of Microbiological Methods
T1  - Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR
VL  - 168
DO  - 10.1016/j.mimet.2019.105786
ER  - 

@article{
author = "Machado-Moreira, Bernardino and Monteiro, Silvia and Santos, Ricardo and Martinez-Murcia, Antonio and Rajković, Andreja and Šmigić, Nada and Richards, Karl G. and Abram, Florence and Burgess, Catherine M.",
year = "2020",
abstract = "There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Microbiological Methods",
title = "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR",
volume = "168",
doi = "10.1016/j.mimet.2019.105786"
}

Machado-Moreira, B., Monteiro, S., Santos, R., Martinez-Murcia, A., Rajković, A., Šmigić, N., Richards, K. G., Abram, F.,& Burgess, C. M.. (2020). Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. in Journal of Microbiological Methods
Elsevier, Amsterdam., 168.
https://doi.org/10.1016/j.mimet.2019.105786

Machado-Moreira B, Monteiro S, Santos R, Martinez-Murcia A, Rajković A, Šmigić N, Richards KG, Abram F, Burgess CM. Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR. in Journal of Microbiological Methods. 2020;168.
doi:10.1016/j.mimet.2019.105786 .

Machado-Moreira, Bernardino, Monteiro, Silvia, Santos, Ricardo, Martinez-Murcia, Antonio, Rajković, Andreja, Šmigić, Nada, Richards, Karl G., Abram, Florence, Burgess, Catherine M., "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR" in Journal of Microbiological Methods, 168 (2020),
https://doi.org/10.1016/j.mimet.2019.105786 . .