TY  - JOUR
AU  - Thien Trung Le
AU  - Miočinović, Jelena
AU  - Tuyet Mai Nguyen
AU  - Rombaut, Roeland
AU  - van Camp, John
AU  - Dewettinck, Koen
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2648
AB  - A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials
EP  - 10413
IS  - 19
SP  - 10407
VL  - 59
DO  - 10.1021/jf200202d
ER  - 

@article{
author = "Thien Trung Le and Miočinović, Jelena and Tuyet Mai Nguyen and Rombaut, Roeland and van Camp, John and Dewettinck, Koen",
year = "2011",
abstract = "A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials",
pages = "10413-10407",
number = "19",
volume = "59",
doi = "10.1021/jf200202d"
}

Thien Trung Le, Miočinović, J., Tuyet Mai Nguyen, Rombaut, R., van Camp, J.,& Dewettinck, K.. (2011). Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 59(19), 10407-10413.
https://doi.org/10.1021/jf200202d

Thien Trung Le, Miočinović J, Tuyet Mai Nguyen, Rombaut R, van Camp J, Dewettinck K. Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials. in Journal of Agricultural and Food Chemistry. 2011;59(19):10407-10413.
doi:10.1021/jf200202d .

Thien Trung Le, Miočinović, Jelena, Tuyet Mai Nguyen, Rombaut, Roeland, van Camp, John, Dewettinck, Koen, "Improved Solvent Extraction Procedure and High-Performance Liquid Chromatography-Evaporative Light-Scattering Detector Method for Analysis of Polar Lipids from Dairy Materials" in Journal of Agricultural and Food Chemistry, 59, no. 19 (2011):10407-10413,
https://doi.org/10.1021/jf200202d . .