TY  - JOUR
AU  - Kałużna, Monika
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
AU  - Weldon, William A.
AU  - Stockwell, Virginia O.
AU  - Pothier, Joël F.
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2023.1254107
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6461
AB  - Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.
T2  - Frontiers in Plant Science
T2  - Frontiers in Plant Science
T1  - Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6461
ER  - 

@article{
author = "Kałużna, Monika and Prokić, Andjelka and Obradović, Aleksa and Weldon, William A. and Stockwell, Virginia O. and Pothier, Joël F.",
year = "2023",
abstract = "Xanthomonas arboricola pv. corylina (Xac; formerly Xanthomonas campestris pv. corylina) is the causal agent of the bacterial blight of hazelnuts, a devastating disease of trees in plant nurseries and young orchards. Currently, there are no PCR assays to distinguish Xac from all other pathovars of X. arboricola. A comparative genomics approach with publicly available genomes of Xac was used to identify unique sequences, conserved across the genomes of the pathogen. We identified a 2,440 bp genomic region that was unique to Xac and designed identification and detection systems for conventional PCR, qPCR (SYBR® Green and TaqMan™), and loop-mediated isothermal amplification (LAMP). All PCR assays performed on genomic DNA isolated from eight X. arboricola pathovars and closely related bacterial species confirmed the specificity of designed primers. These new multi-platform molecular diagnostic tools may be used by plant clinics and researchers to detect and identify Xac in pure cultures and hazelnut tissues rapidly and accurately.",
journal = "Frontiers in Plant Science, Frontiers in Plant Science",
title = "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6461"
}

Kałużna, M., Prokić, A., Obradović, A., Weldon, W. A., Stockwell, V. O.,& Pothier, J. F.. (2023). Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science, 14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461

Kałużna M, Prokić A, Obradović A, Weldon WA, Stockwell VO, Pothier JF. Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics. in Frontiers in Plant Science. 2023;14.
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

Kałużna, Monika, Prokić, Andjelka, Obradović, Aleksa, Weldon, William A., Stockwell, Virginia O., Pothier, Joël F., "Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics" in Frontiers in Plant Science, 14 (2023),
https://hdl.handle.net/21.15107/rcub_agrospace_6461 .

TY  - JOUR
AU  - Popović-Djordjević, Jelena B.
AU  - Kostić, Aleksandar Ž.
AU  - Rajković, Miloš B.
AU  - Miljković, Irena
AU  - Krstić, Đurđa
AU  - Caruso, Gianluca
AU  - Siavash Moghaddam, Sina
AU  - Brčeski, Ilija
PY  - 2022
UR  - https://doi.org/10.1007/s12011-021-02639-9
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6389
AB  - Vegetables are important contributors to a healthy diet, and their adequate daily intake can help prevent some of the major illnesses. The aim of the study was to examine the content of the major and trace elements in selected organically grown (OG) and conventionally grown (CG) vegetables (cabbage, kohlrabi, Brussels sprout, beetroot, carrot, potato, and onion), taken from city green markets. Multi-elemental analysis was carried out by inductively coupled plasma method with optical emission spectrometry (ICP-OES). Nutritional quality evaluation in comparison to nutritional reference values was done. In studied vegetables, Al, Ca, K, Fe (with the exception of organic kohlrabi), Mg, Na, P, S, and Zn were quantified in all samples, whereas As, Cd, Co, Hg, Se, and V were below the limit of detection for these elements. Macroelements and trace elements were found at higher concentrations in OG and CG vegetables, respectively. Differences in concentrations of studied elements between the same vegetable species produced in two agricultural systems were significant, except for beetroot (p ≤ 0.05). Principal component analysis and hierarchical cluster analysis results showed that the botanical origin had higher influence on sample differentiation than the agronomic practice, which was in accordance with the results obtained by Mann-Whitney U test. Good quality of both OG and CG vegetables in respect of nutritionally beneficial elements was observed.
T2  - Biological Trace Element Research
T2  - Biological Trace Element ResearchBiol Trace Elem Res
T1  - Organically vs. Conventionally Grown Vegetables: Multi-elemental Analysis and Nutritional Evaluation
EP  - 436
IS  - 1
SP  - 426
VL  - 200
DO  - 10.1007/s12011-021-02639-9
ER  - 

@article{
author = "Popović-Djordjević, Jelena B. and Kostić, Aleksandar Ž. and Rajković, Miloš B. and Miljković, Irena and Krstić, Đurđa and Caruso, Gianluca and Siavash Moghaddam, Sina and Brčeski, Ilija",
year = "2022",
abstract = "Vegetables are important contributors to a healthy diet, and their adequate daily intake can help prevent some of the major illnesses. The aim of the study was to examine the content of the major and trace elements in selected organically grown (OG) and conventionally grown (CG) vegetables (cabbage, kohlrabi, Brussels sprout, beetroot, carrot, potato, and onion), taken from city green markets. Multi-elemental analysis was carried out by inductively coupled plasma method with optical emission spectrometry (ICP-OES). Nutritional quality evaluation in comparison to nutritional reference values was done. In studied vegetables, Al, Ca, K, Fe (with the exception of organic kohlrabi), Mg, Na, P, S, and Zn were quantified in all samples, whereas As, Cd, Co, Hg, Se, and V were below the limit of detection for these elements. Macroelements and trace elements were found at higher concentrations in OG and CG vegetables, respectively. Differences in concentrations of studied elements between the same vegetable species produced in two agricultural systems were significant, except for beetroot (p ≤ 0.05). Principal component analysis and hierarchical cluster analysis results showed that the botanical origin had higher influence on sample differentiation than the agronomic practice, which was in accordance with the results obtained by Mann-Whitney U test. Good quality of both OG and CG vegetables in respect of nutritionally beneficial elements was observed.",
journal = "Biological Trace Element Research, Biological Trace Element ResearchBiol Trace Elem Res",
title = "Organically vs. Conventionally Grown Vegetables: Multi-elemental Analysis and Nutritional Evaluation",
pages = "436-426",
number = "1",
volume = "200",
doi = "10.1007/s12011-021-02639-9"
}

Popović-Djordjević, J. B., Kostić, A. Ž., Rajković, M. B., Miljković, I., Krstić, Đ., Caruso, G., Siavash Moghaddam, S.,& Brčeski, I.. (2022). Organically vs. Conventionally Grown Vegetables: Multi-elemental Analysis and Nutritional Evaluation. in Biological Trace Element Research, 200(1), 426-436.
https://doi.org/10.1007/s12011-021-02639-9

Popović-Djordjević JB, Kostić AŽ, Rajković MB, Miljković I, Krstić Đ, Caruso G, Siavash Moghaddam S, Brčeski I. Organically vs. Conventionally Grown Vegetables: Multi-elemental Analysis and Nutritional Evaluation. in Biological Trace Element Research. 2022;200(1):426-436.
doi:10.1007/s12011-021-02639-9 .

Popović-Djordjević, Jelena B., Kostić, Aleksandar Ž., Rajković, Miloš B., Miljković, Irena, Krstić, Đurđa, Caruso, Gianluca, Siavash Moghaddam, Sina, Brčeski, Ilija, "Organically vs. Conventionally Grown Vegetables: Multi-elemental Analysis and Nutritional Evaluation" in Biological Trace Element Research, 200, no. 1 (2022):426-436,
https://doi.org/10.1007/s12011-021-02639-9 . .

TY  - JOUR
AU  - Sknepnek, Aleksandra
AU  - Tomić, Sergej
AU  - Miletić, Dunja
AU  - Lević, Steva
AU  - Čolić, Miodrag
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5772
AB  - Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.
PB  - Elsevier
T2  - Food Chemistry
T1  - Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts
SP  - 128344
VL  - 342
DO  - 10.1016/j.foodchem.2020.128344
ER  - 

@article{
author = "Sknepnek, Aleksandra and Tomić, Sergej and Miletić, Dunja and Lević, Steva and Čolić, Miodrag and Nedović, Viktor and Nikšić, Miomir",
year = "2021",
abstract = "Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals. Here we used fruiting bodies to prepare novel kombucha beverage. Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures. FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins. C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract. The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions. The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures. Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts",
pages = "128344",
volume = "342",
doi = "10.1016/j.foodchem.2020.128344"
}

Sknepnek, A., Tomić, S., Miletić, D., Lević, S., Čolić, M., Nedović, V.,& Nikšić, M.. (2021). Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts. in Food Chemistry
Elsevier., 342, 128344.
https://doi.org/10.1016/j.foodchem.2020.128344

Sknepnek A, Tomić S, Miletić D, Lević S, Čolić M, Nedović V, Nikšić M. Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts. in Food Chemistry. 2021;342:128344.
doi:10.1016/j.foodchem.2020.128344 .

Sknepnek, Aleksandra, Tomić, Sergej, Miletić, Dunja, Lević, Steva, Čolić, Miodrag, Nedović, Viktor, Nikšić, Miomir, "Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts" in Food Chemistry, 342 (2021):128344,
https://doi.org/10.1016/j.foodchem.2020.128344 . .