TY  - JOUR
AU  - Jovanović, Gordana D.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4085
AB  - BACKGROUND: The highest incidence of listeriosis, due to consumption of ready-to-eat foods and fresh, shredded, minimally processed vegetables, occurs among pregnant women and the elderly. In order to reduce the prevalence of listeriosis among consumers, better protective measures are recommended. Chitosan films, with or without added essential oils, represent a modern, safe method of preserving the quality of such vegetables and significantly reducing the incidence of Listeria monocytogenes in these foods. Objectives: The present study was conducted to evaluate the antimicrobial properties of composite chitosan-gelatin films with and without essential oils against two strains of L. monocytogenes, ATCC 19115 and ATCC 19112, in fresh shredded cabbage. Methods: Shredded cabbage was inoculated with L. monocytogenes and packed between two layers of the chitosan composite film, then placed in Petri dishes. The prepared samples were stored at 4 degrees C then analyzed for total viable count on PALCAM agar while incubated at 37 degrees C, every 24 hours for 7 days. Results: Average L. monocytogenes content ranged from 4.2 - 5.4 log CFU/g, reaching values of 7.2 - 8.6 log CFU/g in samples of untreated cabbage. A complete reduction of L. monocytogenes ATCC 19115 on cabbage was achieved after 120 hours in the presence of 0.5% chitosan film, whereas reduction of L. monocytogenes ATCC 19112 was achieved after 144 hours. In the presence of 1% chitosan film, the bacteria withered more quickly and complete reduction of both species of L. monocytogenes was achieved after 96 hours. Conclusions: All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of both strains of L. monocytogenes on cabbage. The best effect was achieved with a 1% chitosan concentration. The addition of essential oils increased the antimicrobial activity of all tested films.
PB  - Ahvaz Jundishapur Univ Med Sci, Ahvaz
T2  - Jundishapur Journal of Microbiology
T1  - Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage
IS  - 9
VL  - 9
DO  - 10.5812/jjm.34804
ER  - 

@article{
author = "Jovanović, Gordana D. and Klaus, Anita and Nikšić, Miomir",
year = "2016",
abstract = "BACKGROUND: The highest incidence of listeriosis, due to consumption of ready-to-eat foods and fresh, shredded, minimally processed vegetables, occurs among pregnant women and the elderly. In order to reduce the prevalence of listeriosis among consumers, better protective measures are recommended. Chitosan films, with or without added essential oils, represent a modern, safe method of preserving the quality of such vegetables and significantly reducing the incidence of Listeria monocytogenes in these foods. Objectives: The present study was conducted to evaluate the antimicrobial properties of composite chitosan-gelatin films with and without essential oils against two strains of L. monocytogenes, ATCC 19115 and ATCC 19112, in fresh shredded cabbage. Methods: Shredded cabbage was inoculated with L. monocytogenes and packed between two layers of the chitosan composite film, then placed in Petri dishes. The prepared samples were stored at 4 degrees C then analyzed for total viable count on PALCAM agar while incubated at 37 degrees C, every 24 hours for 7 days. Results: Average L. monocytogenes content ranged from 4.2 - 5.4 log CFU/g, reaching values of 7.2 - 8.6 log CFU/g in samples of untreated cabbage. A complete reduction of L. monocytogenes ATCC 19115 on cabbage was achieved after 120 hours in the presence of 0.5% chitosan film, whereas reduction of L. monocytogenes ATCC 19112 was achieved after 144 hours. In the presence of 1% chitosan film, the bacteria withered more quickly and complete reduction of both species of L. monocytogenes was achieved after 96 hours. Conclusions: All tested formulations of chitosan films exhibited strong antimicrobial activity on the growth of both strains of L. monocytogenes on cabbage. The best effect was achieved with a 1% chitosan concentration. The addition of essential oils increased the antimicrobial activity of all tested films.",
publisher = "Ahvaz Jundishapur Univ Med Sci, Ahvaz",
journal = "Jundishapur Journal of Microbiology",
title = "Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage",
number = "9",
volume = "9",
doi = "10.5812/jjm.34804"
}

Jovanović, G. D., Klaus, A.,& Nikšić, M.. (2016). Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage. in Jundishapur Journal of Microbiology
Ahvaz Jundishapur Univ Med Sci, Ahvaz., 9(9).
https://doi.org/10.5812/jjm.34804

Jovanović GD, Klaus A, Nikšić M. Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage. in Jundishapur Journal of Microbiology. 2016;9(9).
doi:10.5812/jjm.34804 .

Jovanović, Gordana D., Klaus, Anita, Nikšić, Miomir, "Antimicrobial Activity of Chitosan Films With Essential Oils Against Listeria monocytogenes on Cabbage" in Jundishapur Journal of Microbiology, 9, no. 9 (2016),
https://doi.org/10.5812/jjm.34804 . .