TY  - JOUR
AU  - Yan, Y.
AU  - Prodanović, Slaven
AU  - Mladenov, N.
AU  - Milovanović, M.
PY  - 1999
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/147
AB  - Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.
T2  - Cereal Research Communications
T1  - Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis
EP  - 257
IS  - 3
SP  - 251
VL  - 27
DO  - 10.1007/bf03543533
ER  - 

@article{
author = "Yan, Y. and Prodanović, Slaven and Mladenov, N. and Milovanović, M.",
year = "1999",
abstract = "Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.",
journal = "Cereal Research Communications",
title = "Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis",
pages = "257-251",
number = "3",
volume = "27",
doi = "10.1007/bf03543533"
}

Yan, Y., Prodanović, S., Mladenov, N.,& Milovanović, M.. (1999). Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis. in Cereal Research Communications, 27(3), 251-257.
https://doi.org/10.1007/bf03543533

Yan Y, Prodanović S, Mladenov N, Milovanović M. Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis. in Cereal Research Communications. 1999;27(3):251-257.
doi:10.1007/bf03543533 .

Yan, Y., Prodanović, Slaven, Mladenov, N., Milovanović, M., "Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis" in Cereal Research Communications, 27, no. 3 (1999):251-257,
https://doi.org/10.1007/bf03543533 . .

TY  - JOUR
AU  - Yan, Y.
AU  - Liu, G.T.
AU  - Prodanović, Slaven
AU  - Zorić, D.
PY  - 1998
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/139
AB  - Gliadin proteins extracted from seventeen China and Yugoslav winter wheat cultivars were separated by capillary electrophoresis (CE). In order to optimise resolution and reproducibility of gliadin separations, different CE conditions, including capillary length, internal diameter, temperature and voltage, were investigated. Excellent resolution and high reproducibility of gliadin CE patterns were obtained by using a 47 cm length, 50 μm i.d. capillary at 15 kV and 30°C in 0.06 M sodium borate buffer (pH 9.0), containing 20% acetonitrile (ACN) and 1% sodium dodecyl sulphate (SDS). Using these conditions, gliadin electrophoregrams of all cultivars studied showed clear qualitative and quantitative differences, including peak numbers, heights and positions. In particular, some closely related cultivars that were not differentiated by acid polyacrylamide gel electrophoresis (A-PAGE, pH 3.1) were readily differentiated by CE. These results demonstrate the great potential of CE as a new technique for crop varietal identification and seed purity testing.
T2  - Seed Science and Technology
T1  - Discrimination between some Chinese and Yugoslav winter wheat cultivars by capillary electrophoresis of gliadins
EP  - 843
IS  - 3
SP  - 839
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_139
ER  - 

@article{
author = "Yan, Y. and Liu, G.T. and Prodanović, Slaven and Zorić, D.",
year = "1998",
abstract = "Gliadin proteins extracted from seventeen China and Yugoslav winter wheat cultivars were separated by capillary electrophoresis (CE). In order to optimise resolution and reproducibility of gliadin separations, different CE conditions, including capillary length, internal diameter, temperature and voltage, were investigated. Excellent resolution and high reproducibility of gliadin CE patterns were obtained by using a 47 cm length, 50 μm i.d. capillary at 15 kV and 30°C in 0.06 M sodium borate buffer (pH 9.0), containing 20% acetonitrile (ACN) and 1% sodium dodecyl sulphate (SDS). Using these conditions, gliadin electrophoregrams of all cultivars studied showed clear qualitative and quantitative differences, including peak numbers, heights and positions. In particular, some closely related cultivars that were not differentiated by acid polyacrylamide gel electrophoresis (A-PAGE, pH 3.1) were readily differentiated by CE. These results demonstrate the great potential of CE as a new technique for crop varietal identification and seed purity testing.",
journal = "Seed Science and Technology",
title = "Discrimination between some Chinese and Yugoslav winter wheat cultivars by capillary electrophoresis of gliadins",
pages = "843-839",
number = "3",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_139"
}

Yan, Y., Liu, G.T., Prodanović, S.,& Zorić, D.. (1998). Discrimination between some Chinese and Yugoslav winter wheat cultivars by capillary electrophoresis of gliadins. in Seed Science and Technology, 26(3), 839-843.
https://hdl.handle.net/21.15107/rcub_agrospace_139

Yan Y, Liu G, Prodanović S, Zorić D. Discrimination between some Chinese and Yugoslav winter wheat cultivars by capillary electrophoresis of gliadins. in Seed Science and Technology. 1998;26(3):839-843.
https://hdl.handle.net/21.15107/rcub_agrospace_139 .

Yan, Y., Liu, G.T., Prodanović, Slaven, Zorić, D., "Discrimination between some Chinese and Yugoslav winter wheat cultivars by capillary electrophoresis of gliadins" in Seed Science and Technology, 26, no. 3 (1998):839-843,
https://hdl.handle.net/21.15107/rcub_agrospace_139 .