TY  - JOUR
AU  - Živković, Ivana
AU  - Šavikin, Katarina
AU  - Živković, Jelena
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Lazić, Dejan
AU  - Radin, Dragoslava
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5834
AB  - Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.
PB  - Springer
T2  - Plant Foods for Human Nutrition
T1  - Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids
DO  - 10.1007/s11130-021-00895-7
ER  - 

@article{
author = "Živković, Ivana and Šavikin, Katarina and Živković, Jelena and Zdunić, Gordana and Janković, Teodora and Lazić, Dejan and Radin, Dragoslava",
year = "2021",
abstract = "Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.",
publisher = "Springer",
journal = "Plant Foods for Human Nutrition",
title = "Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids",
doi = "10.1007/s11130-021-00895-7"
}

Živković, I., Šavikin, K., Živković, J., Zdunić, G., Janković, T., Lazić, D.,& Radin, D.. (2021). Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids. in Plant Foods for Human Nutrition
Springer..
https://doi.org/10.1007/s11130-021-00895-7

Živković I, Šavikin K, Živković J, Zdunić G, Janković T, Lazić D, Radin D. Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids. in Plant Foods for Human Nutrition. 2021;.
doi:10.1007/s11130-021-00895-7 .

Živković, Ivana, Šavikin, Katarina, Živković, Jelena, Zdunić, Gordana, Janković, Teodora, Lazić, Dejan, Radin, Dragoslava, "Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids" in Plant Foods for Human Nutrition (2021),
https://doi.org/10.1007/s11130-021-00895-7 . .

TY  - JOUR
AU  - Živković, Ivana
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Živković, Jelena
AU  - Bigović, Dubravka
AU  - Menković, Nebojša
AU  - Radin, Dragoslava
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4828
AB  - Medicinal plant extracts (MPE) have a broad antimicrobial activity, including antiviral effects. In our study, eleven dry extract of ten different medicinal plant prepared with two solvents (5%, dimethyl sulfoxide (DMSO) and 30% ethanol in 5% DMSO) in different concentrations have been examined for anti-noroviral activity. The reduction of norovirus > 1 log10 genome equivalents (GE) has been obtained with 1 mg/mL of Aronia melanocarpa leaf extract (AMLE), dried wine Prokupac (WPE) and Hypericum perforatum extract (HyPE). Some of MPE i.e. HyPE, Aronia melanocarpa fruit extract (AMFE) and Punica granatum (PPE) showed better activity when dissolved in 30% ethanol with 5% DMSO. All results of anti-noroviral activity of tested MPE which achieve  lt  0.5 log10 GE are considered as not effective.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Antiviral activity of medicinal plants extracts against foodborne norovirus
EP  - 34
IS  - 38
SP  - 31
DO  - 10.5937/leksir1838031Z
ER  - 

@article{
author = "Živković, Ivana and Šavikin, Katarina and Zdunić, Gordana and Živković, Jelena and Bigović, Dubravka and Menković, Nebojša and Radin, Dragoslava",
year = "2018",
abstract = "Medicinal plant extracts (MPE) have a broad antimicrobial activity, including antiviral effects. In our study, eleven dry extract of ten different medicinal plant prepared with two solvents (5%, dimethyl sulfoxide (DMSO) and 30% ethanol in 5% DMSO) in different concentrations have been examined for anti-noroviral activity. The reduction of norovirus > 1 log10 genome equivalents (GE) has been obtained with 1 mg/mL of Aronia melanocarpa leaf extract (AMLE), dried wine Prokupac (WPE) and Hypericum perforatum extract (HyPE). Some of MPE i.e. HyPE, Aronia melanocarpa fruit extract (AMFE) and Punica granatum (PPE) showed better activity when dissolved in 30% ethanol with 5% DMSO. All results of anti-noroviral activity of tested MPE which achieve  lt  0.5 log10 GE are considered as not effective.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Antiviral activity of medicinal plants extracts against foodborne norovirus",
pages = "34-31",
number = "38",
doi = "10.5937/leksir1838031Z"
}

Živković, I., Šavikin, K., Zdunić, G., Živković, J., Bigović, D., Menković, N.,& Radin, D.. (2018). Antiviral activity of medicinal plants extracts against foodborne norovirus. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 31-34.
https://doi.org/10.5937/leksir1838031Z

Živković I, Šavikin K, Zdunić G, Živković J, Bigović D, Menković N, Radin D. Antiviral activity of medicinal plants extracts against foodborne norovirus. in Lekovite sirovine. 2018;(38):31-34.
doi:10.5937/leksir1838031Z .

Živković, Ivana, Šavikin, Katarina, Zdunić, Gordana, Živković, Jelena, Bigović, Dubravka, Menković, Nebojša, Radin, Dragoslava, "Antiviral activity of medicinal plants extracts against foodborne norovirus" in Lekovite sirovine, no. 38 (2018):31-34,
https://doi.org/10.5937/leksir1838031Z . .