TY  - JOUR
AU  - Brkić, Dragica
AU  - Szakonyne-Pasics, Ilona
AU  - Gašić, Slavica
AU  - Teodorović, Ivana
AU  - Rašković, Božidar
AU  - Brkić, Nenad
AU  - Nesković, Nesko
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3821
AB  - Subacute and subchronic toxicity of the herbicide Avalon (R), a mixture of bentazone and dicamba, were tested on rats. Avalon (R) was administered at dose levels of 250, 500 and 1000 mg/kg body weight/day for 28 and 90 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were monitored together with biochemistry parameters. The results showed that the mixture caused increases in the activities of ALT, AST and ALP, elevated concentrations of sodium, albumin and albumin/globulin ratio in males. In females, ALT activity, cholesterol and phosphate levels were increased. The changes generally were dose related and, in most cases, females exhibited lower susceptibility than males. The effects of a mixture are, in the most cases, different from the effects of the individual substances. The effects of bentazone were not prevalent which would be expected taking the composition of the mixture into account.
PB  - Elsevier, Amsterdam
T2  - Environmental Toxicology and Pharmacology
T1  - Subacute and subchronic toxicity of Avalon (R) mixture (bentazone plus dicamba) to rats
EP  - 1066
IS  - 3
SP  - 1057
VL  - 39
DO  - 10.1016/j.etap.2015.03.004
ER  - 

@article{
author = "Brkić, Dragica and Szakonyne-Pasics, Ilona and Gašić, Slavica and Teodorović, Ivana and Rašković, Božidar and Brkić, Nenad and Nesković, Nesko",
year = "2015",
abstract = "Subacute and subchronic toxicity of the herbicide Avalon (R), a mixture of bentazone and dicamba, were tested on rats. Avalon (R) was administered at dose levels of 250, 500 and 1000 mg/kg body weight/day for 28 and 90 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were monitored together with biochemistry parameters. The results showed that the mixture caused increases in the activities of ALT, AST and ALP, elevated concentrations of sodium, albumin and albumin/globulin ratio in males. In females, ALT activity, cholesterol and phosphate levels were increased. The changes generally were dose related and, in most cases, females exhibited lower susceptibility than males. The effects of a mixture are, in the most cases, different from the effects of the individual substances. The effects of bentazone were not prevalent which would be expected taking the composition of the mixture into account.",
publisher = "Elsevier, Amsterdam",
journal = "Environmental Toxicology and Pharmacology",
title = "Subacute and subchronic toxicity of Avalon (R) mixture (bentazone plus dicamba) to rats",
pages = "1066-1057",
number = "3",
volume = "39",
doi = "10.1016/j.etap.2015.03.004"
}

Brkić, D., Szakonyne-Pasics, I., Gašić, S., Teodorović, I., Rašković, B., Brkić, N.,& Nesković, N.. (2015). Subacute and subchronic toxicity of Avalon (R) mixture (bentazone plus dicamba) to rats. in Environmental Toxicology and Pharmacology
Elsevier, Amsterdam., 39(3), 1057-1066.
https://doi.org/10.1016/j.etap.2015.03.004

Brkić D, Szakonyne-Pasics I, Gašić S, Teodorović I, Rašković B, Brkić N, Nesković N. Subacute and subchronic toxicity of Avalon (R) mixture (bentazone plus dicamba) to rats. in Environmental Toxicology and Pharmacology. 2015;39(3):1057-1066.
doi:10.1016/j.etap.2015.03.004 .

Brkić, Dragica, Szakonyne-Pasics, Ilona, Gašić, Slavica, Teodorović, Ivana, Rašković, Božidar, Brkić, Nenad, Nesković, Nesko, "Subacute and subchronic toxicity of Avalon (R) mixture (bentazone plus dicamba) to rats" in Environmental Toxicology and Pharmacology, 39, no. 3 (2015):1057-1066,
https://doi.org/10.1016/j.etap.2015.03.004 . .

TY  - JOUR
AU  - Tanović, Brankica
AU  - Gašić, Slavica
AU  - Hrustić, Jovana
AU  - Mihajlović, Milica
AU  - Grahovac, Mila
AU  - Delibašić, Goran
AU  - Stevanović, Marija
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3191
AB  - Antifungal activity of thyme essential oil against Monilinia fructigena and development of an effective and stable oil formulation for agricultural use were studied in this paper. Bioactivity of the oil itself and its developed formulation, an emulsifiable concentrate (EC), was tested in vitro and in vivo. In vitro experiments, using a slightly modified agar overlay technique, showed that the initially emulsified thyme essential oil, as well as the developed formulation, significantly inhibited mycelial growth of M. fructigena in vitro. Experiments in vivo, performed on inoculated apple fruits, revealed that the formulation successfully decreased oil evaporation from the treated area and provided a significant level of M. fructigena suppression, 64.7-72.1% compared to the control. To our knowledge, an EC formulation of thyme essential oil for agricultural uses had never been developed before. The presented results are initial findings and evaluation of product activity should be continued in the field to determine its efficacy and activity spectrum, and to estimate the economic aspect of its use.
AB  - U radu je proučavana antifungalna aktivnost etarskog ulja timijana na Monilinia fructigena, kao i mogućnost razvoja efikasne i stabilne formulacije ulja za upotrebu u poljoprivredi. Bioaktivnost etarskog ulja kao i razvijene formulacije - koncentrata za emulziju (EC) testirana je u ogledima in vitro i in vivo. Ogledi in vitro, izvedeni po delimično modifikovanoj metodi testiranja antimikrobne aktivnosti na površini agara, pokazali su da i inicijalno emulgovano ulje i razvijena formulacija značajno inhibiraju porast micelije izolata M. fructigena. U ogledima in vivo na inokulisanim plodovima jabuke pokazano je da je procesom formulisanja značajno smanjena isparljivost ulja sa tretirane površine i postignuta inhibicija razvoja mrke truleži ploda 64,7-72,1% u poređenju sa kontrolom. Koliko nam je poznato, u ovom radu je po prvi put razvijena EC formulacija etarskog ulja timijana za upotrebu u poljoprivredi. Dobijeni rezultati predstavljaju početak istraživanja koje treba dopuniti rezultatima ispitivanja efekata razvijene formulacije u uslovima praktične primene, proučavanja spektra njenog delovanja, kao i ekonomske isplativosti njene primene.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Development of a thyme essential oil formulation and its effect on Monilinia ructigena
T1  - Razvoj formulacije na bazi etarskog ulja timijana i njen uticaj na Monilinia fructigena
EP  - 280
IS  - 4
SP  - 273
VL  - 28
DO  - 10.2298/PIF1304273T
ER  - 

@article{
author = "Tanović, Brankica and Gašić, Slavica and Hrustić, Jovana and Mihajlović, Milica and Grahovac, Mila and Delibašić, Goran and Stevanović, Marija",
year = "2013",
abstract = "Antifungal activity of thyme essential oil against Monilinia fructigena and development of an effective and stable oil formulation for agricultural use were studied in this paper. Bioactivity of the oil itself and its developed formulation, an emulsifiable concentrate (EC), was tested in vitro and in vivo. In vitro experiments, using a slightly modified agar overlay technique, showed that the initially emulsified thyme essential oil, as well as the developed formulation, significantly inhibited mycelial growth of M. fructigena in vitro. Experiments in vivo, performed on inoculated apple fruits, revealed that the formulation successfully decreased oil evaporation from the treated area and provided a significant level of M. fructigena suppression, 64.7-72.1% compared to the control. To our knowledge, an EC formulation of thyme essential oil for agricultural uses had never been developed before. The presented results are initial findings and evaluation of product activity should be continued in the field to determine its efficacy and activity spectrum, and to estimate the economic aspect of its use., U radu je proučavana antifungalna aktivnost etarskog ulja timijana na Monilinia fructigena, kao i mogućnost razvoja efikasne i stabilne formulacije ulja za upotrebu u poljoprivredi. Bioaktivnost etarskog ulja kao i razvijene formulacije - koncentrata za emulziju (EC) testirana je u ogledima in vitro i in vivo. Ogledi in vitro, izvedeni po delimično modifikovanoj metodi testiranja antimikrobne aktivnosti na površini agara, pokazali su da i inicijalno emulgovano ulje i razvijena formulacija značajno inhibiraju porast micelije izolata M. fructigena. U ogledima in vivo na inokulisanim plodovima jabuke pokazano je da je procesom formulisanja značajno smanjena isparljivost ulja sa tretirane površine i postignuta inhibicija razvoja mrke truleži ploda 64,7-72,1% u poređenju sa kontrolom. Koliko nam je poznato, u ovom radu je po prvi put razvijena EC formulacija etarskog ulja timijana za upotrebu u poljoprivredi. Dobijeni rezultati predstavljaju početak istraživanja koje treba dopuniti rezultatima ispitivanja efekata razvijene formulacije u uslovima praktične primene, proučavanja spektra njenog delovanja, kao i ekonomske isplativosti njene primene.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Development of a thyme essential oil formulation and its effect on Monilinia ructigena, Razvoj formulacije na bazi etarskog ulja timijana i njen uticaj na Monilinia fructigena",
pages = "280-273",
number = "4",
volume = "28",
doi = "10.2298/PIF1304273T"
}

Tanović, B., Gašić, S., Hrustić, J., Mihajlović, M., Grahovac, M., Delibašić, G.,& Stevanović, M.. (2013). Development of a thyme essential oil formulation and its effect on Monilinia ructigena. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 28(4), 273-280.
https://doi.org/10.2298/PIF1304273T

Tanović B, Gašić S, Hrustić J, Mihajlović M, Grahovac M, Delibašić G, Stevanović M. Development of a thyme essential oil formulation and its effect on Monilinia ructigena. in Pesticidi i fitomedicina. 2013;28(4):273-280.
doi:10.2298/PIF1304273T .

Tanović, Brankica, Gašić, Slavica, Hrustić, Jovana, Mihajlović, Milica, Grahovac, Mila, Delibašić, Goran, Stevanović, Marija, "Development of a thyme essential oil formulation and its effect on Monilinia ructigena" in Pesticidi i fitomedicina, 28, no. 4 (2013):273-280,
https://doi.org/10.2298/PIF1304273T . .

TY  - CONF
AU  - Brkić, Dragica
AU  - Gašić, Slavica
AU  - Radivojević, Ljiljana
AU  - Szakonyne-Pasics, I.
AU  - Karan, Vesela
AU  - Nešković, Neško
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2669
PB  - Elsevier Ireland Ltd, Clare
C3  - Toxicology Letters
T1  - Avalon (R) (bentazon plus dicamba) herbicide: Subchronic toxicity to rats
EP  - S229
SP  - S228
VL  - 205
DO  - 10.1016/j.toxlet.2011.05.782
ER  - 

@conference{
author = "Brkić, Dragica and Gašić, Slavica and Radivojević, Ljiljana and Szakonyne-Pasics, I. and Karan, Vesela and Nešković, Neško",
year = "2011",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Toxicology Letters",
title = "Avalon (R) (bentazon plus dicamba) herbicide: Subchronic toxicity to rats",
pages = "S229-S228",
volume = "205",
doi = "10.1016/j.toxlet.2011.05.782"
}

Brkić, D., Gašić, S., Radivojević, L., Szakonyne-Pasics, I., Karan, V.,& Nešković, N.. (2011). Avalon (R) (bentazon plus dicamba) herbicide: Subchronic toxicity to rats. in Toxicology Letters
Elsevier Ireland Ltd, Clare., 205, S228-S229.
https://doi.org/10.1016/j.toxlet.2011.05.782

Brkić D, Gašić S, Radivojević L, Szakonyne-Pasics I, Karan V, Nešković N. Avalon (R) (bentazon plus dicamba) herbicide: Subchronic toxicity to rats. in Toxicology Letters. 2011;205:S228-S229.
doi:10.1016/j.toxlet.2011.05.782 .

Brkić, Dragica, Gašić, Slavica, Radivojević, Ljiljana, Szakonyne-Pasics, I., Karan, Vesela, Nešković, Neško, "Avalon (R) (bentazon plus dicamba) herbicide: Subchronic toxicity to rats" in Toxicology Letters, 205 (2011):S228-S229,
https://doi.org/10.1016/j.toxlet.2011.05.782 . .

TY  - JOUR
AU  - Brkić, Dragica
AU  - Szakonyne-Pasics, Ilona
AU  - Gašić, Slavica
AU  - Karan, Vesela
AU  - Radivojević, Ljiljana
AU  - Nešković, Neško
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2533
AB  - Hematological effects of the herbicide Avalon (GAL-57), a mixture of bentazon and dicamba, were tested on rats. Avalon was administered by gavage at three and four dose levels (250, 500, 1000 and 1250 mg/kg weight/day) for 28 and 90 days. Hematological parameters, number of erythrocytes, leukocytes and platelets, hemoglobin concentration, hematocrit and erythrocyte indexes (MCV, MCH and MCHC) were monitored. The results showed that the herbicide Avalon caused decrease in the values of hemoglobin, hematocrit and erythrocyte indexes (both males and females). The changes (mostly) correlated with the doses administered and, in most cases, a lower susceptibility of females than males was observed. The herbicide GAL-57 had no adverse effect on the number of leukocytes, erythrocytes and thrombocytes (both sexes, all doses tested). The results showed that the herbicide Avalon causes weak anemia to the animals tested. Reversibility was apparent during the recovery period of 28 days.
AB  - U radu su ispitivani hematološki efekti herbicida Avalon (GAL-57), koji je mešavina bentazona i dikambe kao aktivnih materija, na pacovima. Preparat je doziran oralnim putem (sondom u želudac) u više nivoa doza (250, 500, 1000 i 1250 mg/kg/telesne mase/dan) tokom 28 i 90 dana. Praćeni su sledeći pokazatelji: broj eritrocita, leukocita i trombocita, koncentracija hemoglobina, hematokrit i eritrocitni indeksi (prosečna zapremina eritrocita, srednja vrednost hemoglobina po eritrocitu i prosečna koncentracija hemoglobina u eritrocitima). Rezultati ovih ispitivanja su pokazali da herbicid Avalon kod oba pola izaziva smanjenje vrednosti hemoglobina, hematokrita i eritrocitnih indeksa, dok kod drugih ispitivanih pokazatelja (broj leukocita, eritrocita i trombocita) nisu registrovane promene u odnosu na kontrolu. Sve promene su, najčešće, u korelaciji sa primenjenim dozama a uočena je, u najvećem broju slučajeva, i nešto manja osetljivost ženki u odnosu na mužjake. Rezultati ispitivanja su, takođe, pokazali da Avalon izaziva blagu anemiju kod ispitivanih životinja s tim da je efekat reverzibilnog karaktera; nakon isteka perioda oporavka od 28 dana vrednosti za sve ispitivane parametre se vraćaju na normalu i ne razlikuju se značajnije u odnosu na kontrolu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Hematological effects of herbicide Avalon® (bentazon+dicamba) in rats
T1  - Hematološki efekti herbicida Avalon® (bentazon+dikamba) za pacova
EP  - 407
IS  - 4
SP  - 401
VL  - 26
DO  - 10.2298/PIF1104401B
ER  - 

@article{
author = "Brkić, Dragica and Szakonyne-Pasics, Ilona and Gašić, Slavica and Karan, Vesela and Radivojević, Ljiljana and Nešković, Neško",
year = "2011",
abstract = "Hematological effects of the herbicide Avalon (GAL-57), a mixture of bentazon and dicamba, were tested on rats. Avalon was administered by gavage at three and four dose levels (250, 500, 1000 and 1250 mg/kg weight/day) for 28 and 90 days. Hematological parameters, number of erythrocytes, leukocytes and platelets, hemoglobin concentration, hematocrit and erythrocyte indexes (MCV, MCH and MCHC) were monitored. The results showed that the herbicide Avalon caused decrease in the values of hemoglobin, hematocrit and erythrocyte indexes (both males and females). The changes (mostly) correlated with the doses administered and, in most cases, a lower susceptibility of females than males was observed. The herbicide GAL-57 had no adverse effect on the number of leukocytes, erythrocytes and thrombocytes (both sexes, all doses tested). The results showed that the herbicide Avalon causes weak anemia to the animals tested. Reversibility was apparent during the recovery period of 28 days., U radu su ispitivani hematološki efekti herbicida Avalon (GAL-57), koji je mešavina bentazona i dikambe kao aktivnih materija, na pacovima. Preparat je doziran oralnim putem (sondom u želudac) u više nivoa doza (250, 500, 1000 i 1250 mg/kg/telesne mase/dan) tokom 28 i 90 dana. Praćeni su sledeći pokazatelji: broj eritrocita, leukocita i trombocita, koncentracija hemoglobina, hematokrit i eritrocitni indeksi (prosečna zapremina eritrocita, srednja vrednost hemoglobina po eritrocitu i prosečna koncentracija hemoglobina u eritrocitima). Rezultati ovih ispitivanja su pokazali da herbicid Avalon kod oba pola izaziva smanjenje vrednosti hemoglobina, hematokrita i eritrocitnih indeksa, dok kod drugih ispitivanih pokazatelja (broj leukocita, eritrocita i trombocita) nisu registrovane promene u odnosu na kontrolu. Sve promene su, najčešće, u korelaciji sa primenjenim dozama a uočena je, u najvećem broju slučajeva, i nešto manja osetljivost ženki u odnosu na mužjake. Rezultati ispitivanja su, takođe, pokazali da Avalon izaziva blagu anemiju kod ispitivanih životinja s tim da je efekat reverzibilnog karaktera; nakon isteka perioda oporavka od 28 dana vrednosti za sve ispitivane parametre se vraćaju na normalu i ne razlikuju se značajnije u odnosu na kontrolu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Hematological effects of herbicide Avalon® (bentazon+dicamba) in rats, Hematološki efekti herbicida Avalon® (bentazon+dikamba) za pacova",
pages = "407-401",
number = "4",
volume = "26",
doi = "10.2298/PIF1104401B"
}

Brkić, D., Szakonyne-Pasics, I., Gašić, S., Karan, V., Radivojević, L.,& Nešković, N.. (2011). Hematological effects of herbicide Avalon® (bentazon+dicamba) in rats. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 401-407.
https://doi.org/10.2298/PIF1104401B

Brkić D, Szakonyne-Pasics I, Gašić S, Karan V, Radivojević L, Nešković N. Hematological effects of herbicide Avalon® (bentazon+dicamba) in rats. in Pesticidi i fitomedicina. 2011;26(4):401-407.
doi:10.2298/PIF1104401B .

Brkić, Dragica, Szakonyne-Pasics, Ilona, Gašić, Slavica, Karan, Vesela, Radivojević, Ljiljana, Nešković, Neško, "Hematological effects of herbicide Avalon® (bentazon+dicamba) in rats" in Pesticidi i fitomedicina, 26, no. 4 (2011):401-407,
https://doi.org/10.2298/PIF1104401B . .

TY  - JOUR
AU  - Brkić, Dragica
AU  - Vitorović, Slavoljub Lj.
AU  - Gašić, Slavica
AU  - Nešković, Neško
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1793
AB  - Carbofuran toxicity on rats was studied during subchronic exposure. Female and male rats were administered carbofuran in drinking water in concentrations of 25, 100 and 400ppm for a period of 90 days. Clinical symptoms, water consumption, body weight gain, organ weight, pathological and histopathological changes in the liver and kidneys were observed and biochemical and haematological examinations were carried out. The results obtained show that carbofuran administered to rats caused a significant decrease in water consumption as well as in brain, serum and erythrocyte cholinesterase activities. Statistically significant increases in relation to the control were found in the serum enzyme activities. The haematological data showed that carbofuran had no significant effect on Hb concentration and total RBC, but total WBC showed a significant statistical decrease. The histopathological changes in liver and kidneys were observed. However, cell regeneration in the liver and kidneys was found in all test groups.
PB  - Elsevier Science Bv, Amsterdam
T2  - Environmental Toxicology and Pharmacology
T1  - Carbofuran in water: Subchronic toxicity to rats
EP  - 341
IS  - 3
SP  - 334
VL  - 25
DO  - 10.1016/j.etap.2007.11.002
ER  - 

@article{
author = "Brkić, Dragica and Vitorović, Slavoljub Lj. and Gašić, Slavica and Nešković, Neško",
year = "2008",
abstract = "Carbofuran toxicity on rats was studied during subchronic exposure. Female and male rats were administered carbofuran in drinking water in concentrations of 25, 100 and 400ppm for a period of 90 days. Clinical symptoms, water consumption, body weight gain, organ weight, pathological and histopathological changes in the liver and kidneys were observed and biochemical and haematological examinations were carried out. The results obtained show that carbofuran administered to rats caused a significant decrease in water consumption as well as in brain, serum and erythrocyte cholinesterase activities. Statistically significant increases in relation to the control were found in the serum enzyme activities. The haematological data showed that carbofuran had no significant effect on Hb concentration and total RBC, but total WBC showed a significant statistical decrease. The histopathological changes in liver and kidneys were observed. However, cell regeneration in the liver and kidneys was found in all test groups.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Environmental Toxicology and Pharmacology",
title = "Carbofuran in water: Subchronic toxicity to rats",
pages = "341-334",
number = "3",
volume = "25",
doi = "10.1016/j.etap.2007.11.002"
}

Brkić, D., Vitorović, S. Lj., Gašić, S.,& Nešković, N.. (2008). Carbofuran in water: Subchronic toxicity to rats. in Environmental Toxicology and Pharmacology
Elsevier Science Bv, Amsterdam., 25(3), 334-341.
https://doi.org/10.1016/j.etap.2007.11.002

Brkić D, Vitorović SL, Gašić S, Nešković N. Carbofuran in water: Subchronic toxicity to rats. in Environmental Toxicology and Pharmacology. 2008;25(3):334-341.
doi:10.1016/j.etap.2007.11.002 .

Brkić, Dragica, Vitorović, Slavoljub Lj., Gašić, Slavica, Nešković, Neško, "Carbofuran in water: Subchronic toxicity to rats" in Environmental Toxicology and Pharmacology, 25, no. 3 (2008):334-341,
https://doi.org/10.1016/j.etap.2007.11.002 . .

TY  - JOUR
AU  - Brkić, Dragica
AU  - Gašić, Slavica
AU  - Béres, Erzsébet
AU  - Pápai, Enikő
AU  - Karan, Vesela
AU  - Nešković, Neško
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1478
AB  - A micronucleus test was run to investigate mutagenic potential of the herbicide GAL-57, a formulated mixture of bentazone and dicamba. The test was applied to mice of both sexes (strain: CRL: NMRI BR) and the herbicide (product) was administered by gavage at 2000 mg/kg rate, twice within 24 hs. Cyclophosphamide (positive control) was administered at 60 mg/kg, while distilled water as a solvent was negative control. The animals were sacrificed 24 hrs after second treatment, their bone marrow cells isolated from femur, and effects evaluated. The data acquired showed that repeated treatment of mice with GAL-57 caused neither biological nor significant statistical increase in the number of micronuclei in treated animals. At the same time, the number of micronucleated polychromatic erythrocytes in the bone marrow of animals treated with cyclophosphamide (positive control) showed a significant statistical increase. The results suggest that the herbicide product tested did not show any mutagenic activity under the conditions of mouse micronucleus test.
AB  - Mutageni potencijal herbicida GAL-57, koji je formulisan kao mešavina bentazona i dikambe, ispitivan je primenom mikronukleus testa. Ispitivanja su rađena na miševima oba pola (soj: CRL: NMRI BR), a ispitivani herbicid (preparat) je doziran oralno (sondom u želudac) u dozi od 2000 mg/kg, dva puta u razmaku od 24 sata. Kao pozitivna kontrola korišćen je ciklofosfamid (60 mg/kg), a kao negativna rastvarač (destilovana voda). Životinje su žrtvovane 24 sata nakon drugog tretiranja, izolovane su ćelije koštane srži (iz femura) i ocenjivan je efekat supstancije. Dobijeni rezultati su pokazali da ponovljen tretman miševa herbicidom GAL-57 ne izaziva ni biološki ni statistički značajno povećanje broja mikronukleusa kod tretiranih životinja. Istovremeno, kod životinja tretiranih ciklofosfamidom (pozitivna kontrola) broj mikronukleusa u polihromatskim eritrocitima koštane srži miševa bio je statistički značajno povećan. Na osnovu ovih rezultata može se zaključiti da ispitivani herbicid (preparat), u uslovima mikronukleus testa, ne ispoljava mutagena svojstva.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Examination of mutagenic effects of GAL-57 herbicide (bentazone+dicamba) using mouse micronucleus test
T1  - Ispitivanja mutagenih svojstava herbicida GAL-57 (bentazon + dikamba) primenom mikronukleus testa
EP  - 260
IS  - 3
SP  - 255
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1478
ER  - 

@article{
author = "Brkić, Dragica and Gašić, Slavica and Béres, Erzsébet and Pápai, Enikő and Karan, Vesela and Nešković, Neško",
year = "2007",
abstract = "A micronucleus test was run to investigate mutagenic potential of the herbicide GAL-57, a formulated mixture of bentazone and dicamba. The test was applied to mice of both sexes (strain: CRL: NMRI BR) and the herbicide (product) was administered by gavage at 2000 mg/kg rate, twice within 24 hs. Cyclophosphamide (positive control) was administered at 60 mg/kg, while distilled water as a solvent was negative control. The animals were sacrificed 24 hrs after second treatment, their bone marrow cells isolated from femur, and effects evaluated. The data acquired showed that repeated treatment of mice with GAL-57 caused neither biological nor significant statistical increase in the number of micronuclei in treated animals. At the same time, the number of micronucleated polychromatic erythrocytes in the bone marrow of animals treated with cyclophosphamide (positive control) showed a significant statistical increase. The results suggest that the herbicide product tested did not show any mutagenic activity under the conditions of mouse micronucleus test., Mutageni potencijal herbicida GAL-57, koji je formulisan kao mešavina bentazona i dikambe, ispitivan je primenom mikronukleus testa. Ispitivanja su rađena na miševima oba pola (soj: CRL: NMRI BR), a ispitivani herbicid (preparat) je doziran oralno (sondom u želudac) u dozi od 2000 mg/kg, dva puta u razmaku od 24 sata. Kao pozitivna kontrola korišćen je ciklofosfamid (60 mg/kg), a kao negativna rastvarač (destilovana voda). Životinje su žrtvovane 24 sata nakon drugog tretiranja, izolovane su ćelije koštane srži (iz femura) i ocenjivan je efekat supstancije. Dobijeni rezultati su pokazali da ponovljen tretman miševa herbicidom GAL-57 ne izaziva ni biološki ni statistički značajno povećanje broja mikronukleusa kod tretiranih životinja. Istovremeno, kod životinja tretiranih ciklofosfamidom (pozitivna kontrola) broj mikronukleusa u polihromatskim eritrocitima koštane srži miševa bio je statistički značajno povećan. Na osnovu ovih rezultata može se zaključiti da ispitivani herbicid (preparat), u uslovima mikronukleus testa, ne ispoljava mutagena svojstva.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Examination of mutagenic effects of GAL-57 herbicide (bentazone+dicamba) using mouse micronucleus test, Ispitivanja mutagenih svojstava herbicida GAL-57 (bentazon + dikamba) primenom mikronukleus testa",
pages = "260-255",
number = "3",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1478"
}

Brkić, D., Gašić, S., Béres, E., Pápai, E., Karan, V.,& Nešković, N.. (2007). Examination of mutagenic effects of GAL-57 herbicide (bentazone+dicamba) using mouse micronucleus test. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 22(3), 255-260.
https://hdl.handle.net/21.15107/rcub_agrospace_1478

Brkić D, Gašić S, Béres E, Pápai E, Karan V, Nešković N. Examination of mutagenic effects of GAL-57 herbicide (bentazone+dicamba) using mouse micronucleus test. in Pesticidi i fitomedicina. 2007;22(3):255-260.
https://hdl.handle.net/21.15107/rcub_agrospace_1478 .

Brkić, Dragica, Gašić, Slavica, Béres, Erzsébet, Pápai, Enikő, Karan, Vesela, Nešković, Neško, "Examination of mutagenic effects of GAL-57 herbicide (bentazone+dicamba) using mouse micronucleus test" in Pesticidi i fitomedicina, 22, no. 3 (2007):255-260,
https://hdl.handle.net/21.15107/rcub_agrospace_1478 .

TY  - JOUR
AU  - Brkić, Dragica
AU  - Gašić, Slavica
AU  - Vértesi, Adél
AU  - Karan, Vesela
AU  - Nešković, Neško
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1190
AB  - Genotoxicity of the herbicide GAL-57, containing two active ingredients, bentazon and dicamba, was investigated using the Ames test. Salmonella typhimurium (tester strains TA98 and TA100) and Escherichia coli (strain WP2uvrA) were used. Nine product concentrations were tested at a range of 19.53-5000 μg/plate and each concentration, as well as the controls, in triplicate. Testing was done with and without metabolic activation (liver microsomal fraction, S-9 mix). The results of our investigation revealed no biological or statistically significant increase in mutagenic factors, and this offered a basis for our conclusion that the herbicide GAL-57 has no genotoxic properties (with or without metabolic activation) under experimental conditions in the Ames test.
AB  - Genotoksična svojstva herbicida GAL-57, koji u svom sastavu ima dve aktivne materije, bentazon i dikambu, ispitivana su primenom Ames-ovog testa. Korišćene su bakterije Salmonella typhimurium (sojevi TA98 i TA100) i Escherichia coli (WP2uvrA soj). Ispitivano je devet koncentracija preparata u opsegu 19.53-5000 μg/ploča pri čemu je svaka koncentracija, kao i kontrole, testirana po tri puta. Ispitivanje je obavljeno sa i bez metaboličke aktivacije (mikrozomska frakcija jetre, S-9 mix). Rezultati ispitivanja su pokazali da nema ni biološki ni statistički značajnog povećanja faktora mutacije na osnovu čega se zaključuje da herbicid GAL-57, u eksperimentalnim uslovima Ames-ovog testa, ne poseduje genotoksična svojstva (sa i bez metaboličke aktivacije).
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Genotoxicity of GAL-57 herbicide in Salmonella typhimurium and Escherichia coli
T1  - Ispitivanja genotoksičnosti herbicida GAL-57 na Salmonella typhimurium i Escherichia coli
EP  - 323
IS  - 4
SP  - 317
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1190
ER  - 

@article{
author = "Brkić, Dragica and Gašić, Slavica and Vértesi, Adél and Karan, Vesela and Nešković, Neško",
year = "2006",
abstract = "Genotoxicity of the herbicide GAL-57, containing two active ingredients, bentazon and dicamba, was investigated using the Ames test. Salmonella typhimurium (tester strains TA98 and TA100) and Escherichia coli (strain WP2uvrA) were used. Nine product concentrations were tested at a range of 19.53-5000 μg/plate and each concentration, as well as the controls, in triplicate. Testing was done with and without metabolic activation (liver microsomal fraction, S-9 mix). The results of our investigation revealed no biological or statistically significant increase in mutagenic factors, and this offered a basis for our conclusion that the herbicide GAL-57 has no genotoxic properties (with or without metabolic activation) under experimental conditions in the Ames test., Genotoksična svojstva herbicida GAL-57, koji u svom sastavu ima dve aktivne materije, bentazon i dikambu, ispitivana su primenom Ames-ovog testa. Korišćene su bakterije Salmonella typhimurium (sojevi TA98 i TA100) i Escherichia coli (WP2uvrA soj). Ispitivano je devet koncentracija preparata u opsegu 19.53-5000 μg/ploča pri čemu je svaka koncentracija, kao i kontrole, testirana po tri puta. Ispitivanje je obavljeno sa i bez metaboličke aktivacije (mikrozomska frakcija jetre, S-9 mix). Rezultati ispitivanja su pokazali da nema ni biološki ni statistički značajnog povećanja faktora mutacije na osnovu čega se zaključuje da herbicid GAL-57, u eksperimentalnim uslovima Ames-ovog testa, ne poseduje genotoksična svojstva (sa i bez metaboličke aktivacije).",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Genotoxicity of GAL-57 herbicide in Salmonella typhimurium and Escherichia coli, Ispitivanja genotoksičnosti herbicida GAL-57 na Salmonella typhimurium i Escherichia coli",
pages = "323-317",
number = "4",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1190"
}

Brkić, D., Gašić, S., Vértesi, A., Karan, V.,& Nešković, N.. (2006). Genotoxicity of GAL-57 herbicide in Salmonella typhimurium and Escherichia coli. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 21(4), 317-323.
https://hdl.handle.net/21.15107/rcub_agrospace_1190

Brkić D, Gašić S, Vértesi A, Karan V, Nešković N. Genotoxicity of GAL-57 herbicide in Salmonella typhimurium and Escherichia coli. in Pesticidi i fitomedicina. 2006;21(4):317-323.
https://hdl.handle.net/21.15107/rcub_agrospace_1190 .

Brkić, Dragica, Gašić, Slavica, Vértesi, Adél, Karan, Vesela, Nešković, Neško, "Genotoxicity of GAL-57 herbicide in Salmonella typhimurium and Escherichia coli" in Pesticidi i fitomedicina, 21, no. 4 (2006):317-323,
https://hdl.handle.net/21.15107/rcub_agrospace_1190 .