@article{
author = "Krnjaja, Vesna and Petrović, Tanja and Stanković, Slavica and Lukić, Miloš and Škrbić, Zdenka and Mandić, Violeta and Bijelić, Zorica",
year = "2019",
abstract = "In this study, a total of 30 poultry (chicken and laying hens) feed samples collected from different poultry farms in Serbia in 2016 were tested for fungal and aflatoxin contamination. Using the plate count and standard mycological methods, total fungal counts and potentially toxigenic fungal genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was detected by ELISA (enzyme-linked immune sorbent assay) method. The total fungal count was in the range from 1 x 102 (2 log CFU g-1 ) to 1.83 x 105 CFU g-1 (5.26 logCFU g-1 ). The majority of the chicken feeds (78.57%) had the total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1 , whereas in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x 105 CFU g -1 . In 21.43% of the chicken feeds fungal contamination reached the level above the regulation limits. Three potentially toxigenic fungal genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the tested poultry feed samples, more samples contaminated with Aspergillus were determined compared to samples contaminated by Fusarium and Penicillium species. The AFB1 was detected in concentrations from 1.34 to 18.29 g kg-1 , with an average of 4.47 and 4.56 g kg-1 in the chicken and laying hens feed samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was above the regulation limits. The obtained results confirmed the importance of continuous mycological and mycotoxicological control of poultry feed, as well as need to improve risk assessments of such contaminants along the food chain., U ovom radu je 30 uzoraka hrane za živinu sakupljenih tokom 2016. godine iz različitih živinarskih farmi u Srbiji, ispitivano na prisustvo gljiva i aflatoksina u uzorku. Primenom metode razrešenja i standardnih mikoloških metoda utvrđeni su ukupan broj gljiva i identifikovani su potencijalno toksigeni rodovi gljiva. Prirodna pojava aflatoksina B1 (AFB1) utvrđena je primenom biohemijske imunoadsorpcione metode (ELISA). Ukupan broj gljiva bio je od 1 x 102 (2 logCFU g-1 ) do 1,83 x 105 CFU g-1 (5.26 log CFU g-1 ). Najveći broj uzoraka hrane za piliće (78,57%) imao je ukupan broj gljiva u rangu od 1 x 102 do 4,8 x 104 CFU g-1 , dok je 68,75% uzoraka hrane za nosilje imalo ukupan broj gljiva u rangu od 5,3 x 104 do 1,83 x 105 CFU g-1 . U 21,43% hrane za piliće ustanovljen je nedozvoljen ukupan broj gljiva. Identifikovana su tri potencijalno toksigena roda gljiva Aspergillus, Fusarium i Penicillium. Najveći broj ispitivanih uzoraka hrane za živinu bio je kontaminiran Aspergillus vrstama, u odnosu na Fusarium i Penicillium vrste koje su kontaminirale manji broj uzoraka. Rang sadržaja AFB1 bio je od 1,34 do 18,29 µg kg-1 , sa prosečnim sadržajem od 4,47 µg kg-1 u uzorcima hrane za piliće, i 4,56 µg kg-1 u uzorcima hrane za nosilje. U 14,29% uzoraka hrane za piliće ustanovljen je nedozvoljen sadržaj AFB1. Dobijeni rezultati potvrđuju značaj stalne mikološke i mikotoksikološke kontrole hrane za živinu, kao i potrebu za usavršavanjem procene rizika od štetnih (gljivičnih) kontaminenata u lancu ishrane.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Mycobiota and aflatoxin B1 in poultry feeds, Mikobiota i aflatoksin B1 u hrani za živinu",
pages = "69-61",
number = "1",
volume = "35",
doi = "10.2298/BAH1901061K"
}
