TY  - JOUR
AU  - Savić, Iva
AU  - Nikolić, Milica
AU  - Nikolić, Ana
AU  - Vico, Ivana
AU  - Mladenović Drinić, Snežana
AU  - Duduk, Nataša
AU  - Stanković, Slavica
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5934
AB  - Savić I., M. Nikolić, A.Nikolić, I. Vico, S. Mladenović Drinić, N. Duduk, S. Stanković (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. - Genetika, Vol 53, No.2, 641-649. During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain
EP  - 649
IS  - 2
SP  - 641
VL  - 53
DO  - 10.2298/GENSR2102641S
ER  - 

@article{
author = "Savić, Iva and Nikolić, Milica and Nikolić, Ana and Vico, Ivana and Mladenović Drinić, Snežana and Duduk, Nataša and Stanković, Slavica",
year = "2021",
abstract = "Savić I., M. Nikolić, A.Nikolić, I. Vico, S. Mladenović Drinić, N. Duduk, S. Stanković (2021). The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain. - Genetika, Vol 53, No.2, 641-649. During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain",
pages = "649-641",
number = "2",
volume = "53",
doi = "10.2298/GENSR2102641S"
}

Savić, I., Nikolić, M., Nikolić, A., Vico, I., Mladenović Drinić, S., Duduk, N.,& Stanković, S.. (2021). The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain. in Genetika
Serbian Genetics Society., 53(2), 641-649.
https://doi.org/10.2298/GENSR2102641S

Savić I, Nikolić M, Nikolić A, Vico I, Mladenović Drinić S, Duduk N, Stanković S. The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain. in Genetika. 2021;53(2):641-649.
doi:10.2298/GENSR2102641S .

Savić, Iva, Nikolić, Milica, Nikolić, Ana, Vico, Ivana, Mladenović Drinić, Snežana, Duduk, Nataša, Stanković, Slavica, "The Morfological And Molecular Identification Of Fusarium Verticillioides Causing Fusariosis On Wheat Grain" in Genetika, 53, no. 2 (2021):641-649,
https://doi.org/10.2298/GENSR2102641S . .

TY  - JOUR
AU  - Božinović, Sofija
AU  - Prodanović, Slaven
AU  - Vančetović, Jelena
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Ignjatović, Dragana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3681
AB  - Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield
EP  - 167
IS  - 2
SP  - 160
VL  - 75
DO  - 10.4067/S0718-58392015000200004
ER  - 

@article{
author = "Božinović, Sofija and Prodanović, Slaven and Vančetović, Jelena and Nikolić, Ana and Ristić, Danijela and Kostadinović, Marija and Ignjatović, Dragana",
year = "2015",
abstract = "Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield",
pages = "167-160",
number = "2",
volume = "75",
doi = "10.4067/S0718-58392015000200004"
}

Božinović, S., Prodanović, S., Vančetović, J., Nikolić, A., Ristić, D., Kostadinović, M.,& Ignjatović, D.. (2015). Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 75(2), 160-167.
https://doi.org/10.4067/S0718-58392015000200004

Božinović S, Prodanović S, Vančetović J, Nikolić A, Ristić D, Kostadinović M, Ignjatović D. Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research. 2015;75(2):160-167.
doi:10.4067/S0718-58392015000200004 .

Božinović, Sofija, Prodanović, Slaven, Vančetović, Jelena, Nikolić, Ana, Ristić, Danijela, Kostadinović, Marija, Ignjatović, Dragana, "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield" in Chilean Journal of Agricultural Research, 75, no. 2 (2015):160-167,
https://doi.org/10.4067/S0718-58392015000200004 . .