TY  - JOUR
AU  - Drvenica, Ivana T.
AU  - Stancić, Ana Z.
AU  - Kalušević, Ana
AU  - Marković, Smilja
AU  - Dragišić-Maksimović, Jelena J.
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Ilić, Vesna Lj
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4996
AB  - Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin
EP  - 1117
IS  - 10
SP  - 1105
VL  - 84
DO  - 10.2298/JSC190513067D
ER  - 

@article{
author = "Drvenica, Ivana T. and Stancić, Ana Z. and Kalušević, Ana and Marković, Smilja and Dragišić-Maksimović, Jelena J. and Nedović, Viktor and Bugarski, Branko and Ilić, Vesna Lj",
year = "2019",
abstract = "Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin",
pages = "1117-1105",
number = "10",
volume = "84",
doi = "10.2298/JSC190513067D"
}

Drvenica, I. T., Stancić, A. Z., Kalušević, A., Marković, S., Dragišić-Maksimović, J. J., Nedović, V., Bugarski, B.,& Ilić, V. L.. (2019). Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 84(10), 1105-1117.
https://doi.org/10.2298/JSC190513067D

Drvenica IT, Stancić AZ, Kalušević A, Marković S, Dragišić-Maksimović JJ, Nedović V, Bugarski B, Ilić VL. Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2019;84(10):1105-1117.
doi:10.2298/JSC190513067D .

Drvenica, Ivana T., Stancić, Ana Z., Kalušević, Ana, Marković, Smilja, Dragišić-Maksimović, Jelena J., Nedović, Viktor, Bugarski, Branko, Ilić, Vesna Lj, "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 84, no. 10 (2019):1105-1117,
https://doi.org/10.2298/JSC190513067D . .