TY  - JOUR
AU  - Petrović, Predrag
AU  - Ivanović, Katarina
AU  - Octrue, Charly
AU  - Tumara, Mica
AU  - Jovanović, Aleksandra
AU  - Vunduk, Jovana
AU  - Nikšić, Miomir
AU  - Pjanović, Rada
AU  - Bugarski, Branko
AU  - Klaus, Anita
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5015
AB  - Chaga (Inonotus obliquus) is a parasitic fungus, which has been used in traditional medicine in Russia and other northern European countries in the treatment of cancer, gastrointestinal andliver diseases. It has been a subject of intensive researchrecently, confirming many of its health-beneficial effects. In order to obtain a product that would allow modified and prolonged release of the Chaga's active metabolites, hot water Chaga extract was immobilized using calcium - alginate. The extract, which was predominantly composed of carbohydrates (57 %), also contained a relatively high amount of antioxidants/phenolic compounds (130 mg gallic acid equivalents per g of dry extract) and exhibited pronounced radical scavenging activity. It showed significant antibacterial activity as well, inhibiting growth of tested bacterial strains at concentrations of 1.25-20 mg/mL. Entrapment efficiency was about 80 %, and the extract-alginate system showed pH-dependant extract release; there was negligible release at pH 1.75 (gastric pH), and the release gradually increased with the increase in pH, reaching similar to 43 % of immobilized extract at pH 8.5 after 90 min. Such a product could be used as a dietary supplement, adjuvant in therapy of gastrointestinal diseases or as a food additive.
PB  - Savez hemijskih inženjera, Beograd
T2  - HEMIJSKA INDUSTRIJA
T1  - Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency
EP  - 335
IS  - 5
SP  - 325
VL  - 73
DO  - 10.2298/HEMIND190819028P
ER  - 

@article{
author = "Petrović, Predrag and Ivanović, Katarina and Octrue, Charly and Tumara, Mica and Jovanović, Aleksandra and Vunduk, Jovana and Nikšić, Miomir and Pjanović, Rada and Bugarski, Branko and Klaus, Anita",
year = "2019",
abstract = "Chaga (Inonotus obliquus) is a parasitic fungus, which has been used in traditional medicine in Russia and other northern European countries in the treatment of cancer, gastrointestinal andliver diseases. It has been a subject of intensive researchrecently, confirming many of its health-beneficial effects. In order to obtain a product that would allow modified and prolonged release of the Chaga's active metabolites, hot water Chaga extract was immobilized using calcium - alginate. The extract, which was predominantly composed of carbohydrates (57 %), also contained a relatively high amount of antioxidants/phenolic compounds (130 mg gallic acid equivalents per g of dry extract) and exhibited pronounced radical scavenging activity. It showed significant antibacterial activity as well, inhibiting growth of tested bacterial strains at concentrations of 1.25-20 mg/mL. Entrapment efficiency was about 80 %, and the extract-alginate system showed pH-dependant extract release; there was negligible release at pH 1.75 (gastric pH), and the release gradually increased with the increase in pH, reaching similar to 43 % of immobilized extract at pH 8.5 after 90 min. Such a product could be used as a dietary supplement, adjuvant in therapy of gastrointestinal diseases or as a food additive.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "HEMIJSKA INDUSTRIJA",
title = "Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency",
pages = "335-325",
number = "5",
volume = "73",
doi = "10.2298/HEMIND190819028P"
}

Petrović, P., Ivanović, K., Octrue, C., Tumara, M., Jovanović, A., Vunduk, J., Nikšić, M., Pjanović, R., Bugarski, B.,& Klaus, A.. (2019). Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency. in HEMIJSKA INDUSTRIJA
Savez hemijskih inženjera, Beograd., 73(5), 325-335.
https://doi.org/10.2298/HEMIND190819028P

Petrović P, Ivanović K, Octrue C, Tumara M, Jovanović A, Vunduk J, Nikšić M, Pjanović R, Bugarski B, Klaus A. Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency. in HEMIJSKA INDUSTRIJA. 2019;73(5):325-335.
doi:10.2298/HEMIND190819028P .

Petrović, Predrag, Ivanović, Katarina, Octrue, Charly, Tumara, Mica, Jovanović, Aleksandra, Vunduk, Jovana, Nikšić, Miomir, Pjanović, Rada, Bugarski, Branko, Klaus, Anita, "Immobilization of Chaga extract in alginate beads for modified release: simplicity meets efficiency" in HEMIJSKA INDUSTRIJA, 73, no. 5 (2019):325-335,
https://doi.org/10.2298/HEMIND190819028P . .

TY  - JOUR
AU  - Balanc, Bojana
AU  - Trifković, Kata
AU  - Djordjević, Verica
AU  - Marković, Smilja
AU  - Pjanović, Rada
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4033
AB  - We reported the design of liposome-loaded Ca-alginate microspheres as a drug delivery system for controlled release of resveratrol. The effect of admixed sucrose and chitosan coating was assessed in terms of physicochemical, thermal and release properties of liposome-in alginate systems with encapsulated resveratrol. The diameter of liposomes produced by proliposome method increased from 412 to 471 nm with addition of sucrose as a cryoprotectant. DSC analysis revealed that phospolipids interact with each other while forming the lipid bilayer and that resveratrol was incorporated within the lipid bilayer, causing destabilizing effect in the two temperature regions (137-202 degrees C and 240-270 degrees C). Liposomes were entrapped within polymer network and remained intact as determined by SEM cross-sectional observation of the microbeads. Liposomes interfered with the thermal behavior of alginate in the temperature region above 220 degrees C. The presence of liposomes decreased the strength of the beads in comparison to placebo beads, according to mechanical tests on compression. Release studies performed in Franz diffusion cell showed the overall resistance to mass transfer one order of magnitude higher (10(6) s/m) than the resistance ascribed solely to the liposomal membrane. The chitosan coating, visible as a dense surface layer (similar to 7 mu m thick) in dry state, caused decrease in encapsulation efficiency of resveratrol (85% vs. 91%) and in size of the particles (d(50) of 387 vs. 440 mu m); the chitosan also caused weakening of the polymer matrix, but increased resistance to drug diffusion (11.4 x 10(5) s/m) in comparison to the uncoated alginate-liposome formulation (9.1 x 10(5) s/m).
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Hydrocolloids
T1  - Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes
EP  - 842
SP  - 832
VL  - 61
DO  - 10.1016/j.foodhyd.2016.07.005
ER  - 

@article{
author = "Balanc, Bojana and Trifković, Kata and Djordjević, Verica and Marković, Smilja and Pjanović, Rada and Nedović, Viktor and Bugarski, Branko",
year = "2016",
abstract = "We reported the design of liposome-loaded Ca-alginate microspheres as a drug delivery system for controlled release of resveratrol. The effect of admixed sucrose and chitosan coating was assessed in terms of physicochemical, thermal and release properties of liposome-in alginate systems with encapsulated resveratrol. The diameter of liposomes produced by proliposome method increased from 412 to 471 nm with addition of sucrose as a cryoprotectant. DSC analysis revealed that phospolipids interact with each other while forming the lipid bilayer and that resveratrol was incorporated within the lipid bilayer, causing destabilizing effect in the two temperature regions (137-202 degrees C and 240-270 degrees C). Liposomes were entrapped within polymer network and remained intact as determined by SEM cross-sectional observation of the microbeads. Liposomes interfered with the thermal behavior of alginate in the temperature region above 220 degrees C. The presence of liposomes decreased the strength of the beads in comparison to placebo beads, according to mechanical tests on compression. Release studies performed in Franz diffusion cell showed the overall resistance to mass transfer one order of magnitude higher (10(6) s/m) than the resistance ascribed solely to the liposomal membrane. The chitosan coating, visible as a dense surface layer (similar to 7 mu m thick) in dry state, caused decrease in encapsulation efficiency of resveratrol (85% vs. 91%) and in size of the particles (d(50) of 387 vs. 440 mu m); the chitosan also caused weakening of the polymer matrix, but increased resistance to drug diffusion (11.4 x 10(5) s/m) in comparison to the uncoated alginate-liposome formulation (9.1 x 10(5) s/m).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Hydrocolloids",
title = "Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes",
pages = "842-832",
volume = "61",
doi = "10.1016/j.foodhyd.2016.07.005"
}

Balanc, B., Trifković, K., Djordjević, V., Marković, S., Pjanović, R., Nedović, V.,& Bugarski, B.. (2016). Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes. in Food Hydrocolloids
Elsevier Sci Ltd, Oxford., 61, 832-842.
https://doi.org/10.1016/j.foodhyd.2016.07.005

Balanc B, Trifković K, Djordjević V, Marković S, Pjanović R, Nedović V, Bugarski B. Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes. in Food Hydrocolloids. 2016;61:832-842.
doi:10.1016/j.foodhyd.2016.07.005 .

Balanc, Bojana, Trifković, Kata, Djordjević, Verica, Marković, Smilja, Pjanović, Rada, Nedović, Viktor, Bugarski, Branko, "Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes" in Food Hydrocolloids, 61 (2016):832-842,
https://doi.org/10.1016/j.foodhyd.2016.07.005 . .

TY  - JOUR
AU  - Pjanović, Rada
AU  - Goosen, MFA
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2000
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/234
AB  - The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the electrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied.
PB  - Edizioni Minerva Medica, Turin
T2  - Minerva Biotecnologica
T1  - Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory
EP  - 248
IS  - 4
SP  - 241
VL  - 12
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_234
ER  - 

@article{
author = "Pjanović, Rada and Goosen, MFA and Nedović, Viktor and Bugarski, Branko",
year = "2000",
abstract = "The mechanism of alginate droplet formation as well as experimental parameters for producing very small polymer microbeads (i,e, less then 100 microns diameter) using an electrostatic droplet generator were investigated, It was found that microbead size was a function of needle diameter, charge arrangement (i,e, electrode geometry and spacing) and strength of electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; After a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament, When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until now of the polymer caused the process to start again, A mathematical model of droplet formation at the electrified needle was developed from an analysis of the forces acting on a charged droplet, and agreed well with experimental results. Finally, to assess the effect of an electric field on animal cell viability, an insect cell suspension was subjected to a high voltage, There was no detectable loss in cell viability after the voltage was applied.",
publisher = "Edizioni Minerva Medica, Turin",
journal = "Minerva Biotecnologica",
title = "Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory",
pages = "248-241",
number = "4",
volume = "12",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_234"
}

Pjanović, R., Goosen, M., Nedović, V.,& Bugarski, B.. (2000). Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory. in Minerva Biotecnologica
Edizioni Minerva Medica, Turin., 12(4), 241-248.
https://hdl.handle.net/21.15107/rcub_agrospace_234

Pjanović R, Goosen M, Nedović V, Bugarski B. Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory. in Minerva Biotecnologica. 2000;12(4):241-248.
https://hdl.handle.net/21.15107/rcub_agrospace_234 .

Pjanović, Rada, Goosen, MFA, Nedović, Viktor, Bugarski, Branko, "Immobilization/encapsulation of cells using electrostatic droplet generation - Experiments and theory" in Minerva Biotecnologica, 12, no. 4 (2000):241-248,
https://hdl.handle.net/21.15107/rcub_agrospace_234 .