TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Živković, Svetlana
AU  - Obradović, Aleksa
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6024
AB  - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.
PB  - MDPI
T2  - Agronomy
T1  - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
IS  - 2
SP  - 235
VL  - 12
DO  - 10.3390/agronomy12020235
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Živković, Svetlana and Obradović, Aleksa",
year = "2022",
abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically important pathogen of watermelon and related plant species worldwide. In the period 2014–2018, several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of 43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR) revealed that studied A. citrulli strains represent the homogeneous population and they clustered together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay revealed that the strains were able to infect other species within the Cucurbitaceae family.",
publisher = "MDPI",
journal = "Agronomy",
title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia",
number = "2",
pages = "235",
volume = "12",
doi = "10.3390/agronomy12020235"
}

Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Živković, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy
MDPI., 12(2), 235.
https://doi.org/10.3390/agronomy12020235

Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Živković S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12(2):235.
doi:10.3390/agronomy12020235 .

Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Živković, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12, no. 2 (2022):235,
https://doi.org/10.3390/agronomy12020235 . .

TY  - JOUR
AU  - Stevanović, Milos
AU  - Ristić, Danijela
AU  - Zivković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5044
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Milos and Ristić, Danijela and Zivković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Zivković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Zivković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Milos, Ristić, Danijela, Zivković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .

TY  - JOUR
AU  - Aleksić, Goran
AU  - Milićević, Zoran
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Delibašić, Goran
AU  - Živković, Svetlana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5229
AB  - The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection.
AB  - Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Efficacy of copper citrate in grapevine disease control
T1  - Efikasnost bakar-citrata u zaštiti vinove loze od bolesti
EP  - 109
IS  - 2
SP  - 103
VL  - 34
DO  - 10.2298/PIF1902103A
ER  - 

@article{
author = "Aleksić, Goran and Milićević, Zoran and Kuzmanović, Slobodan and Starović, Mira and Stevanović, Miloš and Delibašić, Goran and Živković, Svetlana",
year = "2019",
abstract = "The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection., Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Efficacy of copper citrate in grapevine disease control, Efikasnost bakar-citrata u zaštiti vinove loze od bolesti",
pages = "109-103",
number = "2",
volume = "34",
doi = "10.2298/PIF1902103A"
}

Aleksić, G., Milićević, Z., Kuzmanović, S., Starović, M., Stevanović, M., Delibašić, G.,& Živković, S.. (2019). Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(2), 103-109.
https://doi.org/10.2298/PIF1902103A

Aleksić G, Milićević Z, Kuzmanović S, Starović M, Stevanović M, Delibašić G, Živković S. Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina. 2019;34(2):103-109.
doi:10.2298/PIF1902103A .

Aleksić, Goran, Milićević, Zoran, Kuzmanović, Slobodan, Starović, Mira, Stevanović, Miloš, Delibašić, Goran, Živković, Svetlana, "Efficacy of copper citrate in grapevine disease control" in Pesticidi i fitomedicina, 34, no. 2 (2019):103-109,
https://doi.org/10.2298/PIF1902103A . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3556
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 

@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}

Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G

Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .

Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum: The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .

TY  - JOUR
AU  - Delić, Dušica
AU  - Stajković-Srbinović, Olivera
AU  - Živković, Svetlana
AU  - Protić, Nada
AU  - Rasulić, Nataša
AU  - Kuzmanović, Djordje
AU  - Simić, Aleksandar
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3243
AB  - In the greenhouse experiment, the possibility of alfalfa (Medicago sativa L.) growth promotion by inoculation of preceding barley (Hordeum vulgare L.), with plant growth promoting rhizobacteria (PGPR) was examined. The aim of experiment was to select the effective strains as biofertilizer applied in plant rotation. Effects of inoculation with two Azotobacter and two Pseudomonas strains as well as one Sinorhizobium, Enterobacter and Bacillus strain on shoot dry weight and total N content of alfalfa were determined. The results pointed out significant plant growth promotion abilities of strains A1, A2 and P1 which increased alfalfa shoot dry weight over untreated control Ø, by 41, 39 and 35 %, respectively. These three strains increased total N content of alfalfa plants by 34.92 - 40.45% in respect to control Ø. The presented study showed a significant positive influence of preceding barley inoculation with rhizobacteria alone and their mixture on shoot yield and total N content of alfalfa. Results indicated that strains of Azotobacter sp., Pseudomonas sp. and Enterobacter sp. alone can be investigated in further researches as potential agents of biofertilizer for plant growth promotion of alfalfa.
AB  - U eksprimentu u sudovima ispitana je mogućnost poboljšanja rasta lucerke (Medicago sativa L.) pomoću inokulacije ječma (Hordeum vulgare L.) kao preduseva bakterijama koje poboljšavaju rast biljaka (PGPR). Cilj eksperimenta je bio odabiranje efikasnih sojeva koji bi se primenili u plodoredu u formi biološkog đubriva. U radu je korišćeno sedam sojeva koji pripadaju sledećim bakterijskim vrstama: Sinorhizobium meliloti, Bacillus megaterium, Enterobacter sp, Azotobacter sp, kao i vrsti Pseudomonas sp. Efekat inokulacije ječma primenjenim sojevima je određen na osnovu suve nadzemne mase (SDW) i sadržaja ukupnog azota (N) u biljnoj masi lucerke. Rezultati su ukazali na sposobnost nekih sojeva da poboljšaju rast biljaka. Značajno je povećan prinos lucerke u odnosu na kontrolu Ø (lucerka gajena posle neinokulisanog ječma kao preduseva) inokulacijom sojevima Azotobacter-a A1 (41%) i A2 (39%) i Pseudomonas-a P1 (35%). Sadržaj ukupnog N je bio u korelaciji sa vrednostima SDW. Rezultati su ukazali na uticaj inokulacije ječma kao preduseva na prinos lucerke i njen kvalitet kao i da sojevi Azotobacter sp., Pseudomonas sp. i Enterobacter sp. imaju PGPR potencijal što daje osnovu za dalja ispitivanja i mogućnost primene kao bio­đubriva.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria
T1  - Poboljšanje rasta lucerke, Medicago sativa L. - pomoću inokulacije preduseva rizosfernim bakterijama
EP  - 42
IS  - 1
SP  - 35
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3243
ER  - 

@article{
author = "Delić, Dušica and Stajković-Srbinović, Olivera and Živković, Svetlana and Protić, Nada and Rasulić, Nataša and Kuzmanović, Djordje and Simić, Aleksandar",
year = "2013",
abstract = "In the greenhouse experiment, the possibility of alfalfa (Medicago sativa L.) growth promotion by inoculation of preceding barley (Hordeum vulgare L.), with plant growth promoting rhizobacteria (PGPR) was examined. The aim of experiment was to select the effective strains as biofertilizer applied in plant rotation. Effects of inoculation with two Azotobacter and two Pseudomonas strains as well as one Sinorhizobium, Enterobacter and Bacillus strain on shoot dry weight and total N content of alfalfa were determined. The results pointed out significant plant growth promotion abilities of strains A1, A2 and P1 which increased alfalfa shoot dry weight over untreated control Ø, by 41, 39 and 35 %, respectively. These three strains increased total N content of alfalfa plants by 34.92 - 40.45% in respect to control Ø. The presented study showed a significant positive influence of preceding barley inoculation with rhizobacteria alone and their mixture on shoot yield and total N content of alfalfa. Results indicated that strains of Azotobacter sp., Pseudomonas sp. and Enterobacter sp. alone can be investigated in further researches as potential agents of biofertilizer for plant growth promotion of alfalfa., U eksprimentu u sudovima ispitana je mogućnost poboljšanja rasta lucerke (Medicago sativa L.) pomoću inokulacije ječma (Hordeum vulgare L.) kao preduseva bakterijama koje poboljšavaju rast biljaka (PGPR). Cilj eksperimenta je bio odabiranje efikasnih sojeva koji bi se primenili u plodoredu u formi biološkog đubriva. U radu je korišćeno sedam sojeva koji pripadaju sledećim bakterijskim vrstama: Sinorhizobium meliloti, Bacillus megaterium, Enterobacter sp, Azotobacter sp, kao i vrsti Pseudomonas sp. Efekat inokulacije ječma primenjenim sojevima je određen na osnovu suve nadzemne mase (SDW) i sadržaja ukupnog azota (N) u biljnoj masi lucerke. Rezultati su ukazali na sposobnost nekih sojeva da poboljšaju rast biljaka. Značajno je povećan prinos lucerke u odnosu na kontrolu Ø (lucerka gajena posle neinokulisanog ječma kao preduseva) inokulacijom sojevima Azotobacter-a A1 (41%) i A2 (39%) i Pseudomonas-a P1 (35%). Sadržaj ukupnog N je bio u korelaciji sa vrednostima SDW. Rezultati su ukazali na uticaj inokulacije ječma kao preduseva na prinos lucerke i njen kvalitet kao i da sojevi Azotobacter sp., Pseudomonas sp. i Enterobacter sp. imaju PGPR potencijal što daje osnovu za dalja ispitivanja i mogućnost primene kao bio­đubriva.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria, Poboljšanje rasta lucerke, Medicago sativa L. - pomoću inokulacije preduseva rizosfernim bakterijama",
pages = "42-35",
number = "1",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3243"
}

Delić, D., Stajković-Srbinović, O., Živković, S., Protić, N., Rasulić, N., Kuzmanović, D.,& Simić, A.. (2013). Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 64(1), 35-42.
https://hdl.handle.net/21.15107/rcub_agrospace_3243

Delić D, Stajković-Srbinović O, Živković S, Protić N, Rasulić N, Kuzmanović D, Simić A. Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria. in Zaštita bilja. 2013;64(1):35-42.
https://hdl.handle.net/21.15107/rcub_agrospace_3243 .

Delić, Dušica, Stajković-Srbinović, Olivera, Živković, Svetlana, Protić, Nada, Rasulić, Nataša, Kuzmanović, Djordje, Simić, Aleksandar, "Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria" in Zaštita bilja, 64, no. 1 (2013):35-42,
https://hdl.handle.net/21.15107/rcub_agrospace_3243 .

TY  - JOUR
AU  - Delić, Dušica
AU  - Stajković-Srbinović, Olivera
AU  - Živković, Svetlana
AU  - Protić, Nada
AU  - Rasulić, Nataša
AU  - Kuzmanović, Djordje
AU  - Simić, Aleksandar
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2858
AB  - The object of this study was to evaluate the possible PGPR effects of ryegrass inoculation with Pseudomonas sp. strains as well as its coinoculation with Rhizobium trifolii on the yield and quality of Italian ryegrassm, with the aim to select effective strains as biofertilizer. The inoculation effects of PGPR on the yield of Italian Ryegrass cultivar K-29t were examined in pot experiment under greenhouse conditions. The experiment was designed with 3 inoculated treatments with 3 replications in completely randomised system. Inoculation of ryegrass with two Pseudomonas sp. strains LG and L1K alone as well as strain coinoculation with strains L1K and Rhizobium trfolii 459 represented treatments which were compared with control uninoculated treatment-Ø. The response of plant to the inoculation and coinoculation was positive in comparison to uninoculated plants. Results showed a significant positive influence of coinoculation with strains R. trifilii 459 and Pseudomonas sp. LG as well as Pseudomonas sp. L1K alone on shoot yield, N and P contents of Italian ryegrass. Results indicated that Pseudomonas sp. strains L1K and LG alone and in coinoculation with rhizobial strains can be investigated in further researches as potential agent of biofertilizer for plant growth promotion of Italian ryerass.
AB  - U eksperimentu u sudovima ispitivan je PGPR efekat sojeva Pseudomonas sp. kao i koinokulacija sa Rhizobium trifolii 459 na prinos i kvalitet italijanskog ljulja, Lolium multiflorum Lam. kroz prvi i drugi otkos a u cilju selekcije efikasnih sojeva kao biofertilizatora. Kao biljka domaćin, korišćena je sorta italijanskog ljulja K-29. Eksperiment se sastojao od tri tretmana sa inokulacijom i jednim kontrolnim neinokulisanim tretmanom u 3 ponavljanja u kompletno randomiziranom sistemu. Uticaj inokulacije italijanskog ljulja sa pojedinačnim sojevima LG i L1K kao i koinokulacije sa L1K i Rhizobium trfolii 459 ocenjen je kroz analizu određenih parametara (suva nadzemna masa, ukupan sadržaj N i P u suvoj nadzemnoj masi) koji su poređeni sa kontrolnim neinokulisanim tretmanom. Odgovor biljaka na inokulaciju i koinokulaciju je bio pozitivan u poređenju sa kontrolom. Prema rezultatima, koinokulacija ljulja sojevima R. trifilii 459 and Pseudomonas sp. LG kao i inokulacija pojedinačnim sojevima LG i L1K su uticali na značajno povečanje prinosa suve nadzemne mase u oba otkosa ali i sadržaja N i P u nadzemnom delu biljke. Rezultati su ukazali da sojevi Pseudomionasa samostalno ili u inokulaciji sa sojem R. trifolii imaju potencijal za unapređenje rasta italijanskog ljulja i da predstavljaju potencijalne agense biofertilizatora.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria
T1  - Unapređenje rasta italijanskog ljulja (Lolium multiflorum lam.) primenom PGP Rizobakterija
EP  - 99
IS  - 2
SP  - 93
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2858
ER  - 

@article{
author = "Delić, Dušica and Stajković-Srbinović, Olivera and Živković, Svetlana and Protić, Nada and Rasulić, Nataša and Kuzmanović, Djordje and Simić, Aleksandar",
year = "2012",
abstract = "The object of this study was to evaluate the possible PGPR effects of ryegrass inoculation with Pseudomonas sp. strains as well as its coinoculation with Rhizobium trifolii on the yield and quality of Italian ryegrassm, with the aim to select effective strains as biofertilizer. The inoculation effects of PGPR on the yield of Italian Ryegrass cultivar K-29t were examined in pot experiment under greenhouse conditions. The experiment was designed with 3 inoculated treatments with 3 replications in completely randomised system. Inoculation of ryegrass with two Pseudomonas sp. strains LG and L1K alone as well as strain coinoculation with strains L1K and Rhizobium trfolii 459 represented treatments which were compared with control uninoculated treatment-Ø. The response of plant to the inoculation and coinoculation was positive in comparison to uninoculated plants. Results showed a significant positive influence of coinoculation with strains R. trifilii 459 and Pseudomonas sp. LG as well as Pseudomonas sp. L1K alone on shoot yield, N and P contents of Italian ryegrass. Results indicated that Pseudomonas sp. strains L1K and LG alone and in coinoculation with rhizobial strains can be investigated in further researches as potential agent of biofertilizer for plant growth promotion of Italian ryerass., U eksperimentu u sudovima ispitivan je PGPR efekat sojeva Pseudomonas sp. kao i koinokulacija sa Rhizobium trifolii 459 na prinos i kvalitet italijanskog ljulja, Lolium multiflorum Lam. kroz prvi i drugi otkos a u cilju selekcije efikasnih sojeva kao biofertilizatora. Kao biljka domaćin, korišćena je sorta italijanskog ljulja K-29. Eksperiment se sastojao od tri tretmana sa inokulacijom i jednim kontrolnim neinokulisanim tretmanom u 3 ponavljanja u kompletno randomiziranom sistemu. Uticaj inokulacije italijanskog ljulja sa pojedinačnim sojevima LG i L1K kao i koinokulacije sa L1K i Rhizobium trfolii 459 ocenjen je kroz analizu određenih parametara (suva nadzemna masa, ukupan sadržaj N i P u suvoj nadzemnoj masi) koji su poređeni sa kontrolnim neinokulisanim tretmanom. Odgovor biljaka na inokulaciju i koinokulaciju je bio pozitivan u poređenju sa kontrolom. Prema rezultatima, koinokulacija ljulja sojevima R. trifilii 459 and Pseudomonas sp. LG kao i inokulacija pojedinačnim sojevima LG i L1K su uticali na značajno povečanje prinosa suve nadzemne mase u oba otkosa ali i sadržaja N i P u nadzemnom delu biljke. Rezultati su ukazali da sojevi Pseudomionasa samostalno ili u inokulaciji sa sojem R. trifolii imaju potencijal za unapređenje rasta italijanskog ljulja i da predstavljaju potencijalne agense biofertilizatora.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria, Unapređenje rasta italijanskog ljulja (Lolium multiflorum lam.) primenom PGP Rizobakterija",
pages = "99-93",
number = "2",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2858"
}

Delić, D., Stajković-Srbinović, O., Živković, S., Protić, N., Rasulić, N., Kuzmanović, D.,& Simić, A.. (2012). Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 63(2), 93-99.
https://hdl.handle.net/21.15107/rcub_agrospace_2858

Delić D, Stajković-Srbinović O, Živković S, Protić N, Rasulić N, Kuzmanović D, Simić A. Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria. in Zaštita bilja. 2012;63(2):93-99.
https://hdl.handle.net/21.15107/rcub_agrospace_2858 .

Delić, Dušica, Stajković-Srbinović, Olivera, Živković, Svetlana, Protić, Nada, Rasulić, Nataša, Kuzmanović, Djordje, Simić, Aleksandar, "Growth promotion of Italian ryegrass (Lolium multiflorum lam.) by application of plant growth promoting Rhizobacteria" in Zaštita bilja, 63, no. 2 (2012):93-99,
https://hdl.handle.net/21.15107/rcub_agrospace_2858 .

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Miletić, Novica
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2536
AB  - Peach rusty spot has regularly occurred in Serbia in recent years, causing significant economic losses in the production of late maturing cultivars (Summerset, Suncrest, Fayette and O'Henry). Concerns regarding the etiology of this disease have been solved using molecular identification and cross-pathogenicity tests, which have proved that peach rusty spot is caused by Podosphaera leucotricha. The usual recommended strategy for its control, by multiple treatments starting from petal fall phenophase, has not proved satisfactory in agro-ecological conditions in Serbia. After testing the fungicide efficacy, a three-year study was carried out to determine the precise treatment terms and make the control of P. leucotricha more efficient. The investigation was conducted in the conditions of natural infection in a Summerset cultivar orchard at the locality of Bela Crkva, during the period 2006-2008. Nine different variants of both single and mixed treatments were tested, covering six defined peach growth phenophases - from green top, until the phenophase of second fruit falling. In the conditions of high levels of natural infection in the control plots, in all three investigation years the most efficient was the variant in which kresoxim-methyl was applied three times: in green top, pink bud and full bloom phenophases (91.69 - 91.92%), followed by the variant of treatments in the phenophases of rose bud, full bloom and petal fall (86.3-87.87%) and the variant of two treatments, performed in the phenophases of pink bud and full bloom (79.3-83.09%). The other tested variants showed significantly lower efficiency. The results obtained could significantly contribute to better peach fruit production, without losses caused by peach rusty spot which is commercially very important disease in Serbia.
AB  - Rđasta mrežavost plodova breskve poslednjih godina u Srbiji redovno se javlja i u proizvodnji poznih sorti (Summerset, Suncrest, Fayette i O'Henry) nanoseći značajne ekonomske gubitke. Nedoumice u pogledu etiologije ove bolesti razrešene su na osnovu molekularne identifikacije i unakrsnih testova patogenosti čime je dokazano da rđastu mrežavost breskve izaziva Podosphaera leucotricha. Strategija kontrole P. leucotricha primenjena u svetu, po kojoj suzbijanje treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate u agroekološkim uslovima Srbije. Nakon izvršenih ispitivanja efikasnosti fungicida, obavljeno je trogodišnje istraživanje u cilju određivanja preciznih rokova tretiranja breskve radi uspostavljanja efikasne kontrole P. leucotricha. Ispitivanje je obavljeno u periodu od 2006. do 2008. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je devet različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od šest definisanih fenofaza razvoja breskve, počevši od fenofaze zeleni vrh do fenofaze drugo opadanje plodova. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost u sve tri godine ispitivanja ispoljila je varijanta u kojoj je kresoksim- metil primenjivan tri puta, u fenofazi zeleni vrh, roze pupoljak i cvetanje (91,69-91,92%), odmah zatim varijanta u kojoj su izvođeni tretmani u fenofazi roze pupoljak, cvetanje i precvetavanje (86,3-87,87%) i varijanta u okviru koje su bila vršena dva tretmana u fenofazi roze pupoljak i cvetanje (79,3-83,09%). Ostale varijante u ogledu ispoljile su znatno nižu efikasnost. Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve bez gubitaka izazvanih rđastom mrežavosti ploda, ekonomski sve značajnijeg oboljenja u Srbiji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia
T1  - Optimalni rokovi primene fungicida za suzbijanje Podosphaera leucotricha, prouzrokovača rđaste mrežavosti plodova breskve u Srbiji
EP  - 346
IS  - 4
SP  - 337
VL  - 26
DO  - 10.2298/PIF1104337D
ER  - 

@article{
author = "Dolovac, Nenad and Miletić, Novica and Trkulja, Nenad and Pfaf-Dolovac, Erika and Živković, Svetlana and Krstić, Branka and Bulajić, Aleksandra",
year = "2011",
abstract = "Peach rusty spot has regularly occurred in Serbia in recent years, causing significant economic losses in the production of late maturing cultivars (Summerset, Suncrest, Fayette and O'Henry). Concerns regarding the etiology of this disease have been solved using molecular identification and cross-pathogenicity tests, which have proved that peach rusty spot is caused by Podosphaera leucotricha. The usual recommended strategy for its control, by multiple treatments starting from petal fall phenophase, has not proved satisfactory in agro-ecological conditions in Serbia. After testing the fungicide efficacy, a three-year study was carried out to determine the precise treatment terms and make the control of P. leucotricha more efficient. The investigation was conducted in the conditions of natural infection in a Summerset cultivar orchard at the locality of Bela Crkva, during the period 2006-2008. Nine different variants of both single and mixed treatments were tested, covering six defined peach growth phenophases - from green top, until the phenophase of second fruit falling. In the conditions of high levels of natural infection in the control plots, in all three investigation years the most efficient was the variant in which kresoxim-methyl was applied three times: in green top, pink bud and full bloom phenophases (91.69 - 91.92%), followed by the variant of treatments in the phenophases of rose bud, full bloom and petal fall (86.3-87.87%) and the variant of two treatments, performed in the phenophases of pink bud and full bloom (79.3-83.09%). The other tested variants showed significantly lower efficiency. The results obtained could significantly contribute to better peach fruit production, without losses caused by peach rusty spot which is commercially very important disease in Serbia., Rđasta mrežavost plodova breskve poslednjih godina u Srbiji redovno se javlja i u proizvodnji poznih sorti (Summerset, Suncrest, Fayette i O'Henry) nanoseći značajne ekonomske gubitke. Nedoumice u pogledu etiologije ove bolesti razrešene su na osnovu molekularne identifikacije i unakrsnih testova patogenosti čime je dokazano da rđastu mrežavost breskve izaziva Podosphaera leucotricha. Strategija kontrole P. leucotricha primenjena u svetu, po kojoj suzbijanje treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate u agroekološkim uslovima Srbije. Nakon izvršenih ispitivanja efikasnosti fungicida, obavljeno je trogodišnje istraživanje u cilju određivanja preciznih rokova tretiranja breskve radi uspostavljanja efikasne kontrole P. leucotricha. Ispitivanje je obavljeno u periodu od 2006. do 2008. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je devet različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od šest definisanih fenofaza razvoja breskve, počevši od fenofaze zeleni vrh do fenofaze drugo opadanje plodova. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost u sve tri godine ispitivanja ispoljila je varijanta u kojoj je kresoksim- metil primenjivan tri puta, u fenofazi zeleni vrh, roze pupoljak i cvetanje (91,69-91,92%), odmah zatim varijanta u kojoj su izvođeni tretmani u fenofazi roze pupoljak, cvetanje i precvetavanje (86,3-87,87%) i varijanta u okviru koje su bila vršena dva tretmana u fenofazi roze pupoljak i cvetanje (79,3-83,09%). Ostale varijante u ogledu ispoljile su znatno nižu efikasnost. Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve bez gubitaka izazvanih rđastom mrežavosti ploda, ekonomski sve značajnijeg oboljenja u Srbiji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia, Optimalni rokovi primene fungicida za suzbijanje Podosphaera leucotricha, prouzrokovača rđaste mrežavosti plodova breskve u Srbiji",
pages = "346-337",
number = "4",
volume = "26",
doi = "10.2298/PIF1104337D"
}

Dolovac, N., Miletić, N., Trkulja, N., Pfaf-Dolovac, E., Živković, S., Krstić, B.,& Bulajić, A.. (2011). Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 337-346.
https://doi.org/10.2298/PIF1104337D

Dolovac N, Miletić N, Trkulja N, Pfaf-Dolovac E, Živković S, Krstić B, Bulajić A. Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia. in Pesticidi i fitomedicina. 2011;26(4):337-346.
doi:10.2298/PIF1104337D .

Dolovac, Nenad, Miletić, Novica, Trkulja, Nenad, Pfaf-Dolovac, Erika, Živković, Svetlana, Krstić, Branka, Bulajić, Aleksandra, "Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):337-346,
https://doi.org/10.2298/PIF1104337D . .

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Miletić, Novica
AU  - Aleksić, Goran
AU  - Savić, Dušan
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Bulajić, Aleksandra
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2250
AB  - Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O'Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur) for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the untreated control plots, high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial), flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively) while sulfur was the least efficient (82.33% and 80.30%, respectively). Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic information for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures.
AB  - Rđasta mrežavost plodova breskve odavno je poznato i ekonomski značajno oboljenje u mnogim delovima sveta. U Srbiji tokom proteklih godina ovo oboljenje nanosi značajne štete na poznim sortama breskve (Summerset, Suncrest, Fayette i O'Henry). Mada etiologija bolesti nije poznata, brojna istraživanja u mnogim delovima sveta pokušavaju da uspostave strategiju za kontrolu i preporuče odgovarajuće hemijske i druge metode zaštite breskve. U agroekološkim uslovima Srbije strategija primenjena u svetu, da suzbijanje prouzrokovača rđaste mrežavosti treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate. U pojedinim godinama zaraza plodova osetljivih sorti breskve dostiže 100%. U periodu od 2003. do 2004. godine, ispitivana je efikasnost tri fungicida (kresoksim-metil, fluzilazol i elementarni sumpor) u cilju iznalaženja mogućnosti uspešnije zaštite breskve od prouzrokovača rđaste mrežavosti plodova. Ogled je izveden na breskvi, sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Intenzitet zaraze rđaste mrežavosti plodova na osnovu procenta zahvaćene površine plodova, u kontrolnim parcelama kretao se od 33,5% u prvoj do 35,4% u drugoj godini ispitivanja. Tokom obe godine ispitivanja kresoksim-metil ispoljio je najveću efikasnost (90,25% u prvoj, odnosno 91,12% u drugoj godini ispitivanja), nižu fluzilazol (87,28%, odnosno 87,61%) i najnižu elementarni sumpor (82,33%, odnosno 80,30%). Određivanje fungicida sa najefikasnijim delovanjem prema prouzrokovaču rđaste mrežavosti plodova breskve u Srbiji predstavlja preduslov za dalja ispitivanja koja će uključiti pre svega optimizaciju rokova fungicidnih tretmana kao i određivanje drugih agrotehničkih mera kontrole.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Fungicide efficacy in peach rusty spot control in Serbia
T1  - Efikasnost fungicida za suzbijanje prouzrokovača rđaste mrežavosti plodova breskve u Srbiji
EP  - 249
IS  - 3
SP  - 241
VL  - 25
DO  - 10.2298/PIF1003241D
ER  - 

@article{
author = "Dolovac, Nenad and Miletić, Novica and Aleksić, Goran and Savić, Dušan and Živković, Svetlana and Trkulja, Nenad and Bulajić, Aleksandra",
year = "2010",
abstract = "Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O'Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur) for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the untreated control plots, high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial), flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively) while sulfur was the least efficient (82.33% and 80.30%, respectively). Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic information for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures., Rđasta mrežavost plodova breskve odavno je poznato i ekonomski značajno oboljenje u mnogim delovima sveta. U Srbiji tokom proteklih godina ovo oboljenje nanosi značajne štete na poznim sortama breskve (Summerset, Suncrest, Fayette i O'Henry). Mada etiologija bolesti nije poznata, brojna istraživanja u mnogim delovima sveta pokušavaju da uspostave strategiju za kontrolu i preporuče odgovarajuće hemijske i druge metode zaštite breskve. U agroekološkim uslovima Srbije strategija primenjena u svetu, da suzbijanje prouzrokovača rđaste mrežavosti treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate. U pojedinim godinama zaraza plodova osetljivih sorti breskve dostiže 100%. U periodu od 2003. do 2004. godine, ispitivana je efikasnost tri fungicida (kresoksim-metil, fluzilazol i elementarni sumpor) u cilju iznalaženja mogućnosti uspešnije zaštite breskve od prouzrokovača rđaste mrežavosti plodova. Ogled je izveden na breskvi, sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Intenzitet zaraze rđaste mrežavosti plodova na osnovu procenta zahvaćene površine plodova, u kontrolnim parcelama kretao se od 33,5% u prvoj do 35,4% u drugoj godini ispitivanja. Tokom obe godine ispitivanja kresoksim-metil ispoljio je najveću efikasnost (90,25% u prvoj, odnosno 91,12% u drugoj godini ispitivanja), nižu fluzilazol (87,28%, odnosno 87,61%) i najnižu elementarni sumpor (82,33%, odnosno 80,30%). Određivanje fungicida sa najefikasnijim delovanjem prema prouzrokovaču rđaste mrežavosti plodova breskve u Srbiji predstavlja preduslov za dalja ispitivanja koja će uključiti pre svega optimizaciju rokova fungicidnih tretmana kao i određivanje drugih agrotehničkih mera kontrole.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Fungicide efficacy in peach rusty spot control in Serbia, Efikasnost fungicida za suzbijanje prouzrokovača rđaste mrežavosti plodova breskve u Srbiji",
pages = "249-241",
number = "3",
volume = "25",
doi = "10.2298/PIF1003241D"
}

Dolovac, N., Miletić, N., Aleksić, G., Savić, D., Živković, S., Trkulja, N.,& Bulajić, A.. (2010). Fungicide efficacy in peach rusty spot control in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(3), 241-249.
https://doi.org/10.2298/PIF1003241D

Dolovac N, Miletić N, Aleksić G, Savić D, Živković S, Trkulja N, Bulajić A. Fungicide efficacy in peach rusty spot control in Serbia. in Pesticidi i fitomedicina. 2010;25(3):241-249.
doi:10.2298/PIF1003241D .

Dolovac, Nenad, Miletić, Novica, Aleksić, Goran, Savić, Dušan, Živković, Svetlana, Trkulja, Nenad, Bulajić, Aleksandra, "Fungicide efficacy in peach rusty spot control in Serbia" in Pesticidi i fitomedicina, 25, no. 3 (2010):241-249,
https://doi.org/10.2298/PIF1003241D . .

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Obradović, Aleksa
AU  - Milijašević, Svetlana
AU  - Zivković, S.
AU  - Arsenijević, M.
AU  - Vojinović, M.
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1784
AB  - In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanotneles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25 degrees C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - Proceedings of the Eleventh International Workshop on Fire Blight
T1  - Sorbus sp - New host of Erwinia amylovora in Serbia
EP  - +
SP  - 351
VL  - 793
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1784
ER  - 

@conference{
author = "Gavrilović, Veljko and Obradović, Aleksa and Milijašević, Svetlana and Zivković, S. and Arsenijević, M. and Vojinović, M.",
year = "2008",
abstract = "In 1989 Erwinia amylovora was confirmed to be a pathogen of pear and quince in Serbia. Subsequently, apple, firethorn, hawthorn, Mespilus spp., Cotoneaster horisontalis and Cheanotneles spp. were confirmed as hosts of the pathogen. During the summer 2005, fire blight symptoms were observed on Sorbus spp. for the first time in the south-eastern Serbia, near Nis. Disease symptoms included leaf and shoot blight and cankers with purple-brown colouration of necrotic tissue on mature branches. The diseased tissue was macerated and the suspension was streaked on nutrient sucrose agar (NAS) and King's medium B. Typical light grey, levan-positive colonies developed on NAS medium after two day incubation at 25 degrees C. Colonies on King's medium B were white and nonfluorescent. The results of physiological and biochemical tests of the bacterium were as follows: gram negative, oxidative and fermentative metabolism of glucose; oxidase negative, gelatin hydrolysis positive, aesculin hydrolysis negative and acid produced from most carbon sources. In pathogenicity tests, all isolates induced HR in tobacco leaves and necrosis on artificially inoculated pear fruits followed by appearence of bacterial ooze. After inoculation of petioles and shoots of the host plant (Sorbus spp.) initial symptoms were dark green, watersoaked, eliptical spots on inoculated tissues. Leaf and shoot blight resembling the natural infection appeared five to six days after inoculation. The investigated strains reacted positively with antisera specific to E. amylovora in DASI ELISA test. Identity of isolated strains also was confirmed by polymerase chain reaction (PCR). According to the biochemical and physiological characteristics, pathogenicity tests, results of PCR analyses and ELISA test, strains isolated from Sorbus spp. plants were identified as E. amylovora. This is the first report of E. amylovora on Sorbus spp. plants in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "Proceedings of the Eleventh International Workshop on Fire Blight",
title = "Sorbus sp - New host of Erwinia amylovora in Serbia",
pages = "+-351",
volume = "793",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1784"
}

Gavrilović, V., Obradović, A., Milijašević, S., Zivković, S., Arsenijević, M.,& Vojinović, M.. (2008). Sorbus sp - New host of Erwinia amylovora in Serbia. in Proceedings of the Eleventh International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 793, 351-+.
https://hdl.handle.net/21.15107/rcub_agrospace_1784

Gavrilović V, Obradović A, Milijašević S, Zivković S, Arsenijević M, Vojinović M. Sorbus sp - New host of Erwinia amylovora in Serbia. in Proceedings of the Eleventh International Workshop on Fire Blight. 2008;793:351-+.
https://hdl.handle.net/21.15107/rcub_agrospace_1784 .

Gavrilović, Veljko, Obradović, Aleksa, Milijašević, Svetlana, Zivković, S., Arsenijević, M., Vojinović, M., "Sorbus sp - New host of Erwinia amylovora in Serbia" in Proceedings of the Eleventh International Workshop on Fire Blight, 793 (2008):351-+,
https://hdl.handle.net/21.15107/rcub_agrospace_1784 .