TY  - JOUR
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Kostić Kravljanac, Ljiljana
AU  - Jovičić, Dušica
AU  - Dolijanović, Željko
AU  - Savić, Jasna
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5931
AB  - Delivery of micronutrients to plants through seed priming improves seedling vigour and increases crops yields. Two-year filed trial was conducted in Pančevo, Serbia, with aim to study the effect of seed priming with zinc (Zn) on field performance of three maize hybrids on calcareous chernozem deficient in plant available Zn. Seed priming treatments were: control (without priming), water priming and priming with 4 mM zinc sulphate water solu-tion. Seed priming had significant effect on early plant growth, plant height, yield components, grain yield and grain Zn concentra-tion. Zn-priming promoted plant growth and increased final plant height. Across two growing seasons with contrasting precipitation and three tested maize hybrids, Zn-priming resulted in an average increase of grain yield by about 18% compared to control, and by about 8.4% compared to water priming. A significant relationship between plant growth parameters, grain yield components and grain yield was detected. Grain Zn concentration was increased by Zn-priming in two hybrids in the season with less precipitation and in one hybrid in the second season. The results imply that using the seeds with elevated Zn content can improve overall field performance of maize grown on calcareous chernozem.
PB  - Page Press Publications
T2  - Italian Journal of Agronomy
T1  - Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem
IS  - 3
SP  - 1795
VL  - 16
DO  - 10.4081/ija.2021.1795
ER  - 

@article{
author = "Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Kostić Kravljanac, Ljiljana and Jovičić, Dušica and Dolijanović, Željko and Savić, Jasna",
year = "2021",
abstract = "Delivery of micronutrients to plants through seed priming improves seedling vigour and increases crops yields. Two-year filed trial was conducted in Pančevo, Serbia, with aim to study the effect of seed priming with zinc (Zn) on field performance of three maize hybrids on calcareous chernozem deficient in plant available Zn. Seed priming treatments were: control (without priming), water priming and priming with 4 mM zinc sulphate water solu-tion. Seed priming had significant effect on early plant growth, plant height, yield components, grain yield and grain Zn concentra-tion. Zn-priming promoted plant growth and increased final plant height. Across two growing seasons with contrasting precipitation and three tested maize hybrids, Zn-priming resulted in an average increase of grain yield by about 18% compared to control, and by about 8.4% compared to water priming. A significant relationship between plant growth parameters, grain yield components and grain yield was detected. Grain Zn concentration was increased by Zn-priming in two hybrids in the season with less precipitation and in one hybrid in the second season. The results imply that using the seeds with elevated Zn content can improve overall field performance of maize grown on calcareous chernozem.",
publisher = "Page Press Publications",
journal = "Italian Journal of Agronomy",
title = "Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem",
number = "3",
pages = "1795",
volume = "16",
doi = "10.4081/ija.2021.1795"
}

Tamindžić, G., Ignjatov, M., Milošević, D., Nikolić, Z., Kostić Kravljanac, L., Jovičić, D., Dolijanović, Ž.,& Savić, J.. (2021). Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem. in Italian Journal of Agronomy
Page Press Publications., 16(3), 1795.
https://doi.org/10.4081/ija.2021.1795

Tamindžić G, Ignjatov M, Milošević D, Nikolić Z, Kostić Kravljanac L, Jovičić D, Dolijanović Ž, Savić J. Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem. in Italian Journal of Agronomy. 2021;16(3):1795.
doi:10.4081/ija.2021.1795 .

Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Kostić Kravljanac, Ljiljana, Jovičić, Dušica, Dolijanović, Željko, Savić, Jasna, "Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem" in Italian Journal of Agronomy, 16, no. 3 (2021):1795,
https://doi.org/10.4081/ija.2021.1795 . .

TY  - JOUR
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Nastasić, Aleksandra
AU  - Jovičić, Dušica
AU  - Savić, Jasna
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5868
AB  - Good crop establishment is essential for achieving high yield and constraints to good establishment include untimely sowing and low seed quality combined with various adverse growing conditions after sowing. Seed priming is a pre-sowing technique used for the improvement of germination, reduction of the time from sowing to emergence and improvement of emergence uniformity. Various seed priming techniques, such as hydropriming and priming with zinc, are used nowadays to improve crop establishment. The importance of seed priming with zinc for better germination, improved stand establishment, and higher maize yield are well documented. However, there is still a lack of results on the effects of seed priming with water and zinc on seed quality and viability, given that maize seed can be kept in storage for many years without a significant reduction in germination. The study was aimed to evaluate the effects of seed priming with water and Zn on the quality and viability of the maize seed. In order to evaluate the response of four maize hybrids to priming with water (hydropriming) and 4 mM zinc sulphate, primed seeds were subjected to laboratory tests, namely to the germination test, the cold test, and the accelerated aging test. Both priming treatments increased the seed quality, but the beneficial effect of Zn-priming maintained to a larger extent than hydropriming in cold-treated and aged seeds. The negative effects of hydropriming on the viability of the aged seed of hybrid NS 4023 imply a possible limitation to deferred sowing of primed maize seed.
PB  - Institute of Field and Vegetable Crops
T2  - Ratarstvo i Povrtarstvo
T1  - Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]
EP  - 92
IS  - 3
SP  - 87
VL  - 57
DO  - 10.5937/ratpov57-26575
ER  - 

@article{
author = "Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Nastasić, Aleksandra and Jovičić, Dušica and Savić, Jasna",
year = "2021",
abstract = "Good crop establishment is essential for achieving high yield and constraints to good establishment include untimely sowing and low seed quality combined with various adverse growing conditions after sowing. Seed priming is a pre-sowing technique used for the improvement of germination, reduction of the time from sowing to emergence and improvement of emergence uniformity. Various seed priming techniques, such as hydropriming and priming with zinc, are used nowadays to improve crop establishment. The importance of seed priming with zinc for better germination, improved stand establishment, and higher maize yield are well documented. However, there is still a lack of results on the effects of seed priming with water and zinc on seed quality and viability, given that maize seed can be kept in storage for many years without a significant reduction in germination. The study was aimed to evaluate the effects of seed priming with water and Zn on the quality and viability of the maize seed. In order to evaluate the response of four maize hybrids to priming with water (hydropriming) and 4 mM zinc sulphate, primed seeds were subjected to laboratory tests, namely to the germination test, the cold test, and the accelerated aging test. Both priming treatments increased the seed quality, but the beneficial effect of Zn-priming maintained to a larger extent than hydropriming in cold-treated and aged seeds. The negative effects of hydropriming on the viability of the aged seed of hybrid NS 4023 imply a possible limitation to deferred sowing of primed maize seed.",
publisher = "Institute of Field and Vegetable Crops",
journal = "Ratarstvo i Povrtarstvo",
title = "Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]",
pages = "92-87",
number = "3",
volume = "57",
doi = "10.5937/ratpov57-26575"
}

Tamindžić, G., Ignjatov, M., Milošević, D., Nikolić, Z., Nastasić, A., Jovičić, D.,& Savić, J.. (2021). Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]. in Ratarstvo i Povrtarstvo
Institute of Field and Vegetable Crops., 57(3), 87-92.
https://doi.org/10.5937/ratpov57-26575

Tamindžić G, Ignjatov M, Milošević D, Nikolić Z, Nastasić A, Jovičić D, Savić J. Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]. in Ratarstvo i Povrtarstvo. 2021;57(3):87-92.
doi:10.5937/ratpov57-26575 .

Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Nastasić, Aleksandra, Jovičić, Dušica, Savić, Jasna, "Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]" in Ratarstvo i Povrtarstvo, 57, no. 3 (2021):87-92,
https://doi.org/10.5937/ratpov57-26575 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Miljaković, Dragana
AU  - Stanković, Ivana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5867
AB  - Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'- and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.
PB  - Institute of Field and Vegetable Crops
T2  - Ratarstvo i Povrtarstvo
T1  - Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]
EP  - 54
IS  - 2
SP  - 49
VL  - 57
DO  - 10.5937/ratpov57-25745
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Nikolić, Zorica and Tamindžić, Gordana and Miljaković, Dragana and Stanković, Ivana",
year = "2021",
abstract = "Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'- and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.",
publisher = "Institute of Field and Vegetable Crops",
journal = "Ratarstvo i Povrtarstvo",
title = "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]",
pages = "54-49",
number = "2",
volume = "57",
doi = "10.5937/ratpov57-25745"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Nikolić, Z., Tamindžić, G., Miljaković, D.,& Stanković, I.. (2021). Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]. in Ratarstvo i Povrtarstvo
Institute of Field and Vegetable Crops., 57(2), 49-54.
https://doi.org/10.5937/ratpov57-25745

Milošević D, Ignjatov M, Marjanović-Jeromela A, Nikolić Z, Tamindžić G, Miljaković D, Stanković I. Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]. in Ratarstvo i Povrtarstvo. 2021;57(2):49-54.
doi:10.5937/ratpov57-25745 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Nikolić, Zorica, Tamindžić, Gordana, Miljaković, Dragana, Stanković, Ivana, "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]" in Ratarstvo i Povrtarstvo, 57, no. 2 (2021):49-54,
https://doi.org/10.5937/ratpov57-25745 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5315
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Turnip Yellows Virus on Cabbage in Serbia
EP  - 998
IS  - 3
SP  - 997
VL  - 104
DO  - 10.1094/PDIS-08-19-1682-PDN
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Stanković, Ivana and Krstić, Branka",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Turnip Yellows Virus on Cabbage in Serbia",
pages = "998-997",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1682-PDN"
}

Milošević, D., Ignjatov, M., Vlajić, S., Nikolić, Z., Gvozdanović-Varga, J., Stanković, I.,& Krstić, B.. (2020). First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(3), 997-998.
https://doi.org/10.1094/PDIS-08-19-1682-PDN

Milošević D, Ignjatov M, Vlajić S, Nikolić Z, Gvozdanović-Varga J, Stanković I, Krstić B. First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease. 2020;104(3):997-998.
doi:10.1094/PDIS-08-19-1682-PDN .

Milošević, Dragana, Ignjatov, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Stanković, Ivana, Krstić, Branka, "First Report of Turnip Yellows Virus on Cabbage in Serbia" in Plant Disease, 104, no. 3 (2020):997-998,
https://doi.org/10.1094/PDIS-08-19-1682-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Miljaković, Dragana
AU  - Stanković, Ivana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5495
AB  - Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.
AB  - Šafranika (Carthamus tinctorius L.) predstavlja jednu od važnih uljanih kultura koja pripada familiji Asteraceae. Tokom 2015. godine, prikupljeno je 46 uzoraka biljaka šafranike poreklom iz Srbobrana koji su serološki testirani na prisustvo virusa mozaika krastavca (cucumber mosaic virus, CMV), virusa mozaika lucerke (alfalfa mosaic virus, AMV) i virusa mozaika salate (lettuce mosaic virus, LMV) korišćenjem komercijalnih kitova za DAS-ELISA test. U prikupljenim uzorcima dokazano je prisustvo CMV i AMV, dok prisustvo LMV nije dokazano ni u jednom od testiranih uzoraka. Prisustvo CMV je dalje potvrđeno mehaničkim inokulacijama test biljaka Chenopodium quinoa, C. amaranticolor, Nicotianaglutinosa i Datura stramonium kao i na C. tinctorius, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija CMV obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje celog CP gena kao i delove 5' i 3' UTR. U cilju dalje identifikacije, RT-PCR produkti izolata 290Saff i 294Saff su sekvencionisani (MH577791 i MH577792) i upoređeni sa CMV sekvencama dostupnim u GenBank bazi podataka. Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje odabranih izolata u tri podgrupe, IA, IB i II. Izolati CMV iz šafranike poreklom iz Srbije grupisali su se u podgrupu II. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu CMV na biljkama šafranike u Srbiji koji može da nanese veliku štetu u proizvodnji ove biljne vrste, a takođe predstavlja pretnju drugim ekonomski značajnim gajenim biljnim vrstama u Srbiji.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia
T1  - Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji
EP  - 54
IS  - 2
SP  - 49
VL  - 57
DO  - 10.5937/ratpov57-25745
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Nikolić, Zorica and Tamindžić, Gordana and Miljaković, Dragana and Stanković, Ivana",
year = "2020",
abstract = "Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia., Šafranika (Carthamus tinctorius L.) predstavlja jednu od važnih uljanih kultura koja pripada familiji Asteraceae. Tokom 2015. godine, prikupljeno je 46 uzoraka biljaka šafranike poreklom iz Srbobrana koji su serološki testirani na prisustvo virusa mozaika krastavca (cucumber mosaic virus, CMV), virusa mozaika lucerke (alfalfa mosaic virus, AMV) i virusa mozaika salate (lettuce mosaic virus, LMV) korišćenjem komercijalnih kitova za DAS-ELISA test. U prikupljenim uzorcima dokazano je prisustvo CMV i AMV, dok prisustvo LMV nije dokazano ni u jednom od testiranih uzoraka. Prisustvo CMV je dalje potvrđeno mehaničkim inokulacijama test biljaka Chenopodium quinoa, C. amaranticolor, Nicotianaglutinosa i Datura stramonium kao i na C. tinctorius, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija CMV obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje celog CP gena kao i delove 5' i 3' UTR. U cilju dalje identifikacije, RT-PCR produkti izolata 290Saff i 294Saff su sekvencionisani (MH577791 i MH577792) i upoređeni sa CMV sekvencama dostupnim u GenBank bazi podataka. Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje odabranih izolata u tri podgrupe, IA, IB i II. Izolati CMV iz šafranike poreklom iz Srbije grupisali su se u podgrupu II. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu CMV na biljkama šafranike u Srbiji koji može da nanese veliku štetu u proizvodnji ove biljne vrste, a takođe predstavlja pretnju drugim ekonomski značajnim gajenim biljnim vrstama u Srbiji.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia, Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji",
pages = "54-49",
number = "2",
volume = "57",
doi = "10.5937/ratpov57-25745"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Nikolić, Z., Tamindžić, G., Miljaković, D.,& Stanković, I.. (2020). Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 57(2), 49-54.
https://doi.org/10.5937/ratpov57-25745

Milošević D, Ignjatov M, Marjanović-Jeromela A, Nikolić Z, Tamindžić G, Miljaković D, Stanković I. Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia. in Ratarstvo i povrtarstvo. 2020;57(2):49-54.
doi:10.5937/ratpov57-25745 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Nikolić, Zorica, Tamindžić, Gordana, Miljaković, Dragana, Stanković, Ivana, "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia" in Ratarstvo i povrtarstvo, 57, no. 2 (2020):49-54,
https://doi.org/10.5937/ratpov57-25745 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Krstić, Branka
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5234
AB  - In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
AB  - Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
T1  - Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
EP  - 87
IS  - 3
SP  - 82
VL  - 56
DO  - 10.5937/ratpov56-23514
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Stanković, Ivana and Nikolić, Zorica and Tamindžić, Gordana and Krstić, Branka",
year = "2019",
abstract = "In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank., Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia, Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji",
pages = "87-82",
number = "3",
volume = "56",
doi = "10.5937/ratpov56-23514"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Stanković, I., Nikolić, Z., Tamindžić, G.,& Krstić, B.. (2019). Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 56(3), 82-87.
https://doi.org/10.5937/ratpov56-23514

Milošević D, Ignjatov M, Marjanović-Jeromela A, Stanković I, Nikolić Z, Tamindžić G, Krstić B. Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo. 2019;56(3):82-87.
doi:10.5937/ratpov56-23514 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Stanković, Ivana, Nikolić, Zorica, Tamindžić, Gordana, Krstić, Branka, "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia" in Ratarstvo i povrtarstvo, 56, no. 3 (2019):82-87,
https://doi.org/10.5937/ratpov56-23514 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4880
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Occurrence and diversity of viruses infecting pepper in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 155
IS  - 46
SP  - 141
VL  - 23
DO  - 10.5937/AASer1846141M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Stanković, Ivana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Krstić, Branka",
year = "2018",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Occurrence and diversity of viruses infecting pepper in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "155-141",
number = "46",
volume = "23",
doi = "10.5937/AASer1846141M"
}

Milošević, D., Ignjatov, M., Stanković, I., Nikolić, Z., Gvozdanović-Varga, J.,& Krstić, B.. (2018). Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 23(46), 141-155.
https://doi.org/10.5937/AASer1846141M

Milošević D, Ignjatov M, Stanković I, Nikolić Z, Gvozdanović-Varga J, Krstić B. Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica. 2018;23(46):141-155.
doi:10.5937/AASer1846141M .

Milošević, Dragana, Ignjatov, Maja, Stanković, Ivana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Krstić, Branka, "Occurrence and diversity of viruses infecting pepper in Serbia" in Acta agriculturae Serbica, 23, no. 46 (2018):141-155,
https://doi.org/10.5937/AASer1846141M . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Krstić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4539
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Presence and distribution of pepper viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 656
IS  - 6
SP  - 647
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4539
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Ignjatov, Maja and Nikolić, Zorica and Krstić, Branka",
year = "2017",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Presence and distribution of pepper viruses in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "656-647",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4539"
}

Milošević, D., Stanković, I., Ignjatov, M., Nikolić, Z.,& Krstić, B.. (2017). Presence and distribution of pepper viruses in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 647-656.
https://hdl.handle.net/21.15107/rcub_agrospace_4539

Milošević D, Stanković I, Ignjatov M, Nikolić Z, Krstić B. Presence and distribution of pepper viruses in Serbia. in Biljni lekar. 2017;45(6):647-656.
https://hdl.handle.net/21.15107/rcub_agrospace_4539 .

Milošević, Dragana, Stanković, Ivana, Ignjatov, Maja, Nikolić, Zorica, Krstić, Branka, "Presence and distribution of pepper viruses in Serbia" in Biljni lekar, 45, no. 6 (2017):647-656,
https://hdl.handle.net/21.15107/rcub_agrospace_4539 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Marjanović-Jeromela, Ana
AU  - Krstić, Branka
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4234
AB  - A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.
AB  - Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia
T1  - Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji
EP  - 44
IS  - 1-2
SP  - 37
VL  - 31
DO  - 10.2298/PIF1602037M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Stanković, Ivana and Bulajić, Aleksandra and Marjanović-Jeromela, Ana and Krstić, Branka",
year = "2016",
abstract = "A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates., Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia, Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji",
pages = "44-37",
number = "1-2",
volume = "31",
doi = "10.2298/PIF1602037M"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Stanković, I., Bulajić, A., Marjanović-Jeromela, A.,& Krstić, B.. (2016). The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 31(1-2), 37-44.
https://doi.org/10.2298/PIF1602037M

Milošević D, Ignjatov M, Nikolić Z, Stanković I, Bulajić A, Marjanović-Jeromela A, Krstić B. The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina. 2016;31(1-2):37-44.
doi:10.2298/PIF1602037M .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Stanković, Ivana, Bulajić, Aleksandra, Marjanović-Jeromela, Ana, Krstić, Branka, "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia" in Pesticidi i fitomedicina, 31, no. 1-2 (2016):37-44,
https://doi.org/10.2298/PIF1602037M . .

TY  - JOUR
AU  - Tamindžić, Gordana
AU  - Nikolić, Zorica T.
AU  - Savić, Jasna
AU  - Milošević, Dragana
AU  - Petrović, Gordana R.
AU  - Ivanović, Dragana
AU  - Ignjatov, Maja
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4219
AB  - Seed treatment is a common way of fungicide and insecticide use nowadays, since this way of pesticide application can provide the best protection in the vicinity of the future plant. The aim of research was to evaluate the effects of different seed treatments on germination and seedling growth in three maize inbred lines. The research included the seed treatment with several combinations of a fungicide Maxim XL 035-FS (a.i. metalaxil-M + fludioxonil) and neonicotinoid insecticides Gaucho 600-FS (a.i. imidacloprid) and Cruiser 350-FS (a.i. thiamethoxam), as well as untreated seed (control). The results indicated that inbred lines 21202 x 21101 NS and 317659 NS had a highly vigourous seed which was not affected by the seed treatments. The seed treatments with Maxim XL 035- FS+Gaucho 600-FS and Maxim XL 035-FS+Cruiser 350-FS led to a decrease in germination (90.25% and 89.50%, respectively) of maize inbred line 306081 NS as well as a decrease in root length (126.75 mm and 125.25 mm, respectively) and dry root weight (0.135 g and 0.1875 g, respectively) of maize inbred line 21202 x 21101 NS. All seed treatments had positive effects on root growth, as well as on fresh root weight of maize inbred lines 306081 NS and 317659 NS.
AB  - Tretiranje semena fungicidima i insekticidima je uobičajen način pripreme semena pre setve, s obzirom na to da ovaj način primene pesticida može da pruži najbolju zaštitu mlade biljke od patogena i štetočina u zemljištu. Cilj ovog istraživanja bio je da se ispita uticaj različitih tretmana semena pesticidima na klijanje semena i početni porast izdanaka različitih inbred linija kukuruza. Istraživanje je obuhvatilo testiranje tretmana semena inbred linija kukuruza sa nekoliko kombinacija fungicida (Maxim XL 035-FS, fludioksonil, 25 g/l + metalaksil-M, 10 g/l) i insekticida (Gaucho 600-FS, imidakloprid 600 g/l i Cruiser 350-FS, tiametoksam, 350 g/l). Netretirano seme je uzeto za kontrolu. Rezultati istraživanja su pokazali da inbred linije 21202 x 21101 NS i 317659 NS imaju visoko vigorozno seme, na koje nisu uticali testirani preparati. Testirane kombinacije preparata pesticida Maxim XL 035-FS+Gaucho 600-FS i Maxim XL 035-FS+Cruiser 350-FS dovele su do smanjenja klijavosti semena inbred linije 306081 NS (90,25%, odnosno 89,50%), kao i do smanjenja dužine korena (126,75 mm, odnosno 125,25 mm) i mase suvog korena (0,135 g, odnosno 0,1875 g) izdanaka inbred linije 21202 x 21101 NS. Svi testirani tretmani imali su pozitivan uticaj na porast korena izdanaka, kao i na masu svežeg korena izdanaka inbred linija 306081 NS i 317659 NS.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides
T1  - Porast izdanaka inbred linija kukuruza (Zea mays L.) pod uticajem tretmana semena pesticidima
EP  - 235
IS  - 3
SP  - 227
VL  - 61
DO  - 10.2298/JAS1603227T
ER  - 

@article{
author = "Tamindžić, Gordana and Nikolić, Zorica T. and Savić, Jasna and Milošević, Dragana and Petrović, Gordana R. and Ivanović, Dragana and Ignjatov, Maja",
year = "2016",
abstract = "Seed treatment is a common way of fungicide and insecticide use nowadays, since this way of pesticide application can provide the best protection in the vicinity of the future plant. The aim of research was to evaluate the effects of different seed treatments on germination and seedling growth in three maize inbred lines. The research included the seed treatment with several combinations of a fungicide Maxim XL 035-FS (a.i. metalaxil-M + fludioxonil) and neonicotinoid insecticides Gaucho 600-FS (a.i. imidacloprid) and Cruiser 350-FS (a.i. thiamethoxam), as well as untreated seed (control). The results indicated that inbred lines 21202 x 21101 NS and 317659 NS had a highly vigourous seed which was not affected by the seed treatments. The seed treatments with Maxim XL 035- FS+Gaucho 600-FS and Maxim XL 035-FS+Cruiser 350-FS led to a decrease in germination (90.25% and 89.50%, respectively) of maize inbred line 306081 NS as well as a decrease in root length (126.75 mm and 125.25 mm, respectively) and dry root weight (0.135 g and 0.1875 g, respectively) of maize inbred line 21202 x 21101 NS. All seed treatments had positive effects on root growth, as well as on fresh root weight of maize inbred lines 306081 NS and 317659 NS., Tretiranje semena fungicidima i insekticidima je uobičajen način pripreme semena pre setve, s obzirom na to da ovaj način primene pesticida može da pruži najbolju zaštitu mlade biljke od patogena i štetočina u zemljištu. Cilj ovog istraživanja bio je da se ispita uticaj različitih tretmana semena pesticidima na klijanje semena i početni porast izdanaka različitih inbred linija kukuruza. Istraživanje je obuhvatilo testiranje tretmana semena inbred linija kukuruza sa nekoliko kombinacija fungicida (Maxim XL 035-FS, fludioksonil, 25 g/l + metalaksil-M, 10 g/l) i insekticida (Gaucho 600-FS, imidakloprid 600 g/l i Cruiser 350-FS, tiametoksam, 350 g/l). Netretirano seme je uzeto za kontrolu. Rezultati istraživanja su pokazali da inbred linije 21202 x 21101 NS i 317659 NS imaju visoko vigorozno seme, na koje nisu uticali testirani preparati. Testirane kombinacije preparata pesticida Maxim XL 035-FS+Gaucho 600-FS i Maxim XL 035-FS+Cruiser 350-FS dovele su do smanjenja klijavosti semena inbred linije 306081 NS (90,25%, odnosno 89,50%), kao i do smanjenja dužine korena (126,75 mm, odnosno 125,25 mm) i mase suvog korena (0,135 g, odnosno 0,1875 g) izdanaka inbred linije 21202 x 21101 NS. Svi testirani tretmani imali su pozitivan uticaj na porast korena izdanaka, kao i na masu svežeg korena izdanaka inbred linija 306081 NS i 317659 NS.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides, Porast izdanaka inbred linija kukuruza (Zea mays L.) pod uticajem tretmana semena pesticidima",
pages = "235-227",
number = "3",
volume = "61",
doi = "10.2298/JAS1603227T"
}

Tamindžić, G., Nikolić, Z. T., Savić, J., Milošević, D., Petrović, G. R., Ivanović, D.,& Ignjatov, M.. (2016). Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 61(3), 227-235.
https://doi.org/10.2298/JAS1603227T

Tamindžić G, Nikolić ZT, Savić J, Milošević D, Petrović GR, Ivanović D, Ignjatov M. Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides. in Journal of Agricultural Sciences (Belgrade). 2016;61(3):227-235.
doi:10.2298/JAS1603227T .

Tamindžić, Gordana, Nikolić, Zorica T., Savić, Jasna, Milošević, Dragana, Petrović, Gordana R., Ivanović, Dragana, Ignjatov, Maja, "Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides" in Journal of Agricultural Sciences (Belgrade), 61, no. 3 (2016):227-235,
https://doi.org/10.2298/JAS1603227T . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Petrović, Gordana R.
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3684
AB  - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Detection and molecular characterization of pepper mild mottle virus in Serbia
EP  - 663
IS  - 2
SP  - 651
VL  - 47
DO  - 10.2298/GENSR1502651M
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana R. and Krstić, Branka",
year = "2015",
abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Detection and molecular characterization of pepper mild mottle virus in Serbia",
pages = "663-651",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502651M"
}

Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G. R.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika
Društvo genetičara Srbije, Beograd., 47(2), 651-663.
https://doi.org/10.2298/GENSR1502651M

Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović GR, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika. 2015;47(2):651-663.
doi:10.2298/GENSR1502651M .

Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana R., Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika, 47, no. 2 (2015):651-663,
https://doi.org/10.2298/GENSR1502651M . .

TY  - JOUR
AU  - Milošević, D.
AU  - Marjanović-Jeromela, Ana
AU  - Jovicić, D.
AU  - Ignjatov, Maja
AU  - Nikolić, Z.
AU  - Terzić, Sreten
AU  - Stanković, Ivana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3698
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Alfalfa mosaic virus on Safflower in Serbia
EP  - 896
IS  - 6
SP  - 896
VL  - 99
DO  - 10.1094/PDIS-12-14-1267-PDN
ER  - 

@article{
author = "Milošević, D. and Marjanović-Jeromela, Ana and Jovicić, D. and Ignjatov, Maja and Nikolić, Z. and Terzić, Sreten and Stanković, Ivana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Alfalfa mosaic virus on Safflower in Serbia",
pages = "896-896",
number = "6",
volume = "99",
doi = "10.1094/PDIS-12-14-1267-PDN"
}

Milošević, D., Marjanović-Jeromela, A., Jovicić, D., Ignjatov, M., Nikolić, Z., Terzić, S.,& Stanković, I.. (2015). First Report of Alfalfa mosaic virus on Safflower in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(6), 896-896.
https://doi.org/10.1094/PDIS-12-14-1267-PDN

Milošević D, Marjanović-Jeromela A, Jovicić D, Ignjatov M, Nikolić Z, Terzić S, Stanković I. First Report of Alfalfa mosaic virus on Safflower in Serbia. in Plant Disease. 2015;99(6):896-896.
doi:10.1094/PDIS-12-14-1267-PDN .

Milošević, D., Marjanović-Jeromela, Ana, Jovicić, D., Ignjatov, Maja, Nikolić, Z., Terzić, Sreten, Stanković, Ivana, "First Report of Alfalfa mosaic virus on Safflower in Serbia" in Plant Disease, 99, no. 6 (2015):896-896,
https://doi.org/10.1094/PDIS-12-14-1267-PDN . .

TY  - JOUR
AU  - Milošević, D.
AU  - Ignjatov, Maja
AU  - Nikolić, Z.
AU  - Gvozdanović-Varga, Jelica
AU  - Petrović, Gordana R.
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3700
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia
EP  - 736
IS  - 5
SP  - 736
VL  - 99
DO  - 10.1094/PDIS-11-14-1208-PDN
ER  - 

@article{
author = "Milošević, D. and Ignjatov, Maja and Nikolić, Z. and Gvozdanović-Varga, Jelica and Petrović, Gordana R. and Stanković, Ivana and Krstić, Branka",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia",
pages = "736-736",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1208-PDN"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Petrović, G. R., Stanković, I.,& Krstić, B.. (2015). First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 736-736.
https://doi.org/10.1094/PDIS-11-14-1208-PDN

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Petrović GR, Stanković I, Krstić B. First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia. in Plant Disease. 2015;99(5):736-736.
doi:10.1094/PDIS-11-14-1208-PDN .

Milošević, D., Ignjatov, Maja, Nikolić, Z., Gvozdanović-Varga, Jelica, Petrović, Gordana R., Stanković, Ivana, Krstić, Branka, "First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia" in Plant Disease, 99, no. 5 (2015):736-736,
https://doi.org/10.1094/PDIS-11-14-1208-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tamindžić, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3947
AB  - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II.
AB  - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
T1  - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
EP  - 73
IS  - 2
SP  - 67
VL  - 52
DO  - 10.5937/ratpov52-7814
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji",
pages = "73-67",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-7814"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73.
https://doi.org/10.5937/ratpov52-7814

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo. 2015;52(2):67-73.
doi:10.5937/ratpov52-7814 .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo, 52, no. 2 (2015):67-73,
https://doi.org/10.5937/ratpov52-7814 . .

TY  - JOUR
AU  - Milošević, D.
AU  - Gvozdanović-Varga, Jelica
AU  - Ignjatov, Maja
AU  - Nikolić, Z.
AU  - Vucurović, I.
AU  - Vučurović, Ana
AU  - Stanković, Ivana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3670
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Onion yellow dwarf virus Infecting Shallot in Serbia.
EP  - 1450
IS  - 10
SP  - 1450
VL  - 99
DO  - 10.1094/PDIS-04-15-0370-PDN
ER  - 

@article{
author = "Milošević, D. and Gvozdanović-Varga, Jelica and Ignjatov, Maja and Nikolić, Z. and Vucurović, I. and Vučurović, Ana and Stanković, Ivana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Onion yellow dwarf virus Infecting Shallot in Serbia.",
pages = "1450-1450",
number = "10",
volume = "99",
doi = "10.1094/PDIS-04-15-0370-PDN"
}

Milošević, D., Gvozdanović-Varga, J., Ignjatov, M., Nikolić, Z., Vucurović, I., Vučurović, A.,& Stanković, I.. (2015). First Report of Onion yellow dwarf virus Infecting Shallot in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(10), 1450-1450.
https://doi.org/10.1094/PDIS-04-15-0370-PDN

Milošević D, Gvozdanović-Varga J, Ignjatov M, Nikolić Z, Vucurović I, Vučurović A, Stanković I. First Report of Onion yellow dwarf virus Infecting Shallot in Serbia.. in Plant Disease. 2015;99(10):1450-1450.
doi:10.1094/PDIS-04-15-0370-PDN .

Milošević, D., Gvozdanović-Varga, Jelica, Ignjatov, Maja, Nikolić, Z., Vucurović, I., Vučurović, Ana, Stanković, Ivana, "First Report of Onion yellow dwarf virus Infecting Shallot in Serbia." in Plant Disease, 99, no. 10 (2015):1450-1450,
https://doi.org/10.1094/PDIS-04-15-0370-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Milošević, Dragana
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2937
AB  - Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease.
AB  - Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Sensitivity of some pepper genotypes to bacterial spot causal agent
T1  - Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti
EP  - 182
IS  - 2
SP  - 177
VL  - 49
DO  - 10.5937/ratpov49-1769
ER  - 

@article{
author = "Ignjatov, Maja and Šević, Milan and Gašić, Katarina and Jovičić, Dušica and Nikolić, Zorica and Milošević, Dragana and Obradović, Aleksa",
year = "2012",
abstract = "Bacterial spot, caused by either Xanthomonas euvesicatoria (Xe-Group A), X. vesicatoria (Xv-Group B), X. perforans (Xp-group C) and X. gardneri (Xg-Group D), formerly known as X. campestris pv. vesicatoria, is considered one of the most common pepper and tomato diseases in Serbia. So far, 11 physiological races of X. euvesicatoria bacterium were described around the world. However, four of them (P1, P3, P7, P8) have been recorded in Serbia, predominating being P8. The aim of this study was to examine the sensitivity of the 11 pepper genotypes: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 and Boni to X. euvesicatoria (RKFB 263), race P8. The experiments were carried out in a greenhouse. Pepper plants, with five fully expanded leaves, were artificially inoculated by dipping into the bacterial suspension, concentration of 106 cfu/ml and 108 cfu/ml, for 10 seconds. Early Calwonder (ECW) was used as susceptible control genotype, and its isogenic line ECW-20 withBs2 resistance gene as a resistant control. Experiments were organized as randomized block design with four replications, with five plants in each repetition. The intensity of infection was assessed by Horsfall - Barratt (HB) scale 7 and 14 days after inoculation. According to the results, pepper genotypes showed various degree of susceptibility to X. euvesicatoria (P8) differentiating into significantly different groups. Among the tested cultivars, Bihar F1 showed the highest degree of resistance to the pathogen, while all the other genotypes showed various degrees of sensitivity compared to the controls. Considering that most of the studied genotypes were sensitive to X. euvesicatoria (P8), with exception of the isogenic line ECW-20 with Bs2 resistance gene, transfer of this gene into commercial varieties of pepper would be a significant contribution to control of this economically important disease., Prema najnovijoj sistematici bakterioznu pegavost paprike i paradajzaprouzrokuju vrste Xanthomonas kompleksa: Xanthomonas euvesicatoria, Xanthomonas vesicatoria, Xanthomonas perforans i Xanthomonas gardneri. Do sada je u svetu opisano 11 fizioloških rasa bakterije Xanthomonas euvesicatoria, od kojih je u našoj zemlji prisutno četiri (P1, P3, P7, P8), a rasa P8 je najzastupljenija. Cilj ovog rada bio je proučavanje osetljivosti 11 odabranih genotipovapaprike: HS-2, Amfora, Plamena, Anita, Novosađanka, Palanačka babura, Palanačko čudo, Slonovo uvo, Brillant F1, Bihar F1 i Boni, prema rasi 8 X. euvesicatoria. Kao osetljiva prema svim rasama patogena korišćena je sorta Early Calwonder (ECW), a kao nosilac gena otpornosti Bs2 prema genu avirulentnosti patogena (avrBs2) njena izogena linija ECW-20. Izvedena su dva ogleda, a za veštačku inokulaciju biljaka paprike pripremljene su suspenzije bakterija koncentracije 106 cfu/ml i 108 cfu/ml soja X. euvesicatoria (RKFB 263), rase P8. Veštačka inokulacija biljaka paprike izvedena je u stadijumu šest potpuno razvijenih listova, metodom potapanja biljaka. Ogledi su postavljeni po potpuno slučajnom blok rasporedu u četiri ponavljanja sa po pet biljaka u svakom ponavljanju. Intenzitet zaraze ocenjen je po Horsfall - Barratt (HB) skali 7 i 14 dana nakon inokulacije. Proučavani genotipovi paprike ispoljili su različit stepen osetljivosti prema bakteriji X. euvesicatoria. Nakon ocena intenziteta zaraze u oba ogleda izdvojile su se statistički značajno različite grupe. Samo je hibrid Bihar F1 ispoljio određeni stepen otpornosti prema bakteriji, dok su svi ostali proučavani genotipovi pokazali viši ili niži stepen osetljivosti u odnosu na kontrolne sorte. Obzirom da se većina proučavanih genotipova pokazala kao osetljiva prema prouzrokovaču bakteriozne pegavosti, kao i da postoji izolovan gen otpornosti prema najzastupljenijoj rasi patogena u nas, njegov transfer u komercijalne sorte paprike bio bi značajan doprinos kontroli ovog ekonomski važnog oboljenja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Sensitivity of some pepper genotypes to bacterial spot causal agent, Proučavanje osetljivosti odabranih genotipova paprike prema prouzrokovaču bakteriozne pegavosti",
pages = "182-177",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1769"
}

Ignjatov, M., Šević, M., Gašić, K., Jovičić, D., Nikolić, Z., Milošević, D.,& Obradović, A.. (2012). Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 177-182.
https://doi.org/10.5937/ratpov49-1769

Ignjatov M, Šević M, Gašić K, Jovičić D, Nikolić Z, Milošević D, Obradović A. Sensitivity of some pepper genotypes to bacterial spot causal agent. in Ratarstvo i povrtarstvo. 2012;49(2):177-182.
doi:10.5937/ratpov49-1769 .

Ignjatov, Maja, Šević, Milan, Gašić, Katarina, Jovičić, Dušica, Nikolić, Zorica, Milošević, Dragana, Obradović, Aleksa, "Sensitivity of some pepper genotypes to bacterial spot causal agent" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):177-182,
https://doi.org/10.5937/ratpov49-1769 . .

TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Katić, Slobodan
AU  - Vasiljević, Sanja
AU  - Nikolić, Zorica
AU  - Branković, Gordana
AU  - Čalić, Irena
AU  - Milić, Dragan
AU  - Mikić, Aleksandar
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2284
AB  - Breeding of perennial legumes for many agronomic important traits like grain yield, persistence, longevity, resistance to diseases and pests, resistance to limiting abiotic conditions and polyploidy is more efficient and precise if genetic markers are used. Estimates based on isozyme polymorphism may underestimate overall levels of genetic variation because they are sampling only coding regions of DNA that may be conserved to maintain the function of the enzymes. The complete coverage of a genome can be achieved only by the use of molecular variability indicators (DNA polymorphism), i.e. molecular markers. Molecular markers are independent of environmental influences and can be detected in all plant development stages. The main aspects of genetic markers use in perennial legumes breeding are: germplasm characterisation, genetic linkage mapping, QTL analysis, marker assisted selection (MAS), variety identification and protection of plant breeders' rights.
AB  - Oplemenjivanje višegodišnjih leguminoza za mnoge agronomski značajne osobine kao što su prinos semena, perzistentnost, dugovečnost, otpornost na bolesti i štetočine, otpornost na limitirajuće abiotičke uslove i pojavu poliploidije efikasnije je i preciznije ako se u radu primenjuju i genetički markeri. Polimorfi zam genotipova zasnovan na profi lima izoenzima može da potceni ukupan nivo genetičke varijanse jer se odnosi na kodirajuće regione DNK koji su bili konzervirani kroz evoluciju da bi se očuvala funkcija enzima. Kompletna pokrivenost genoma može se ostvariti samo primenom pokazatelja molekularne varijabilnosti (DNK polimorfizam) tj. molekularnim markerima. Pored toga, molekularni markeri ne zavise od uslova spoljašnje sredine i mogu se detektovati u svim stadijumima fazama razvića biljaka. Glavni aspekti primene genetičkih markera u oplemenjivanju višegodišnjih leguminoza se odnose na: karakterizaciju germplazme, mapiranje vezanih gena, QTL analizu, selekciju uz pomoć markera (MAS), identifikaciju sorata i zaštitu prava oplemenjivača.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Use of genetic markers in breeding of perennial legumes
T1  - Primena genetičkih markera u oplemenjivanju višegodišnjih leguminoza
EP  - 424
IS  - 2
SP  - 417
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2284
ER  - 

@article{
author = "Šurlan-Momirović, Gordana and Katić, Slobodan and Vasiljević, Sanja and Nikolić, Zorica and Branković, Gordana and Čalić, Irena and Milić, Dragan and Mikić, Aleksandar",
year = "2010",
abstract = "Breeding of perennial legumes for many agronomic important traits like grain yield, persistence, longevity, resistance to diseases and pests, resistance to limiting abiotic conditions and polyploidy is more efficient and precise if genetic markers are used. Estimates based on isozyme polymorphism may underestimate overall levels of genetic variation because they are sampling only coding regions of DNA that may be conserved to maintain the function of the enzymes. The complete coverage of a genome can be achieved only by the use of molecular variability indicators (DNA polymorphism), i.e. molecular markers. Molecular markers are independent of environmental influences and can be detected in all plant development stages. The main aspects of genetic markers use in perennial legumes breeding are: germplasm characterisation, genetic linkage mapping, QTL analysis, marker assisted selection (MAS), variety identification and protection of plant breeders' rights., Oplemenjivanje višegodišnjih leguminoza za mnoge agronomski značajne osobine kao što su prinos semena, perzistentnost, dugovečnost, otpornost na bolesti i štetočine, otpornost na limitirajuće abiotičke uslove i pojavu poliploidije efikasnije je i preciznije ako se u radu primenjuju i genetički markeri. Polimorfi zam genotipova zasnovan na profi lima izoenzima može da potceni ukupan nivo genetičke varijanse jer se odnosi na kodirajuće regione DNK koji su bili konzervirani kroz evoluciju da bi se očuvala funkcija enzima. Kompletna pokrivenost genoma može se ostvariti samo primenom pokazatelja molekularne varijabilnosti (DNK polimorfizam) tj. molekularnim markerima. Pored toga, molekularni markeri ne zavise od uslova spoljašnje sredine i mogu se detektovati u svim stadijumima fazama razvića biljaka. Glavni aspekti primene genetičkih markera u oplemenjivanju višegodišnjih leguminoza se odnose na: karakterizaciju germplazme, mapiranje vezanih gena, QTL analizu, selekciju uz pomoć markera (MAS), identifikaciju sorata i zaštitu prava oplemenjivača.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Use of genetic markers in breeding of perennial legumes, Primena genetičkih markera u oplemenjivanju višegodišnjih leguminoza",
pages = "424-417",
number = "2",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2284"
}

Šurlan-Momirović, G., Katić, S., Vasiljević, S., Nikolić, Z., Branković, G., Čalić, I., Milić, D.,& Mikić, A.. (2010). Use of genetic markers in breeding of perennial legumes. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(2), 417-424.
https://hdl.handle.net/21.15107/rcub_agrospace_2284

Šurlan-Momirović G, Katić S, Vasiljević S, Nikolić Z, Branković G, Čalić I, Milić D, Mikić A. Use of genetic markers in breeding of perennial legumes. in Ratarstvo i povrtarstvo. 2010;47(2):417-424.
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Šurlan-Momirović, Gordana, Katić, Slobodan, Vasiljević, Sanja, Nikolić, Zorica, Branković, Gordana, Čalić, Irena, Milić, Dragan, Mikić, Aleksandar, "Use of genetic markers in breeding of perennial legumes" in Ratarstvo i povrtarstvo, 47, no. 2 (2010):417-424,
https://hdl.handle.net/21.15107/rcub_agrospace_2284 .