TY  - JOUR
AU  - Nikolić, Dušan
AU  - Poleksić, Vesna
AU  - Tasić, Aleksandra
AU  - Smederevac-Lalić, Marija
AU  - Djikanović, Vesna
AU  - Rašković, Božidar
PY  - 2023
UR  - https://econpapers.repec.org/article/gamjsusta/v_3a15_3ay_3a2023_3ai_3a14_3ap_3a11321-_3ad_3a1198759.htm
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6415
AB  - Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia) to analyze their bioindicator potential and compare their possible differences. Concentrations of 26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS). Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health. Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some 4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in both age classes. Histopathological changes and indices were minor and did not differ significantly between age classes, suggesting that pollution did not affect the morphology and structure of gills and liver. There were no significant correlations between elemental accumulation and fish condition or between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.
T2  - Sustainability
T2  - Sustainability
T1  - Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution
EP  - 13
IS  - 14
SP  - 1
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6415
ER  - 

@article{
author = "Nikolić, Dušan and Poleksić, Vesna and Tasić, Aleksandra and Smederevac-Lalić, Marija and Djikanović, Vesna and Rašković, Božidar",
year = "2023",
abstract = "Pikeperch of age classes 3+ and 4+ were collected from the Garaši reservoir (Serbia) to analyze their bioindicator potential and compare their possible differences. Concentrations of 26 elements were determined in gills, muscles, and liver by inductively-coupled plasma optical emission spectrometry (ICP-OES) and of 17 organochlorine pesticides (OCPs), and six polychlorinated biphenyls (PCBs) in muscle by gas chromatography with mass spectrometric detection (GC-MS). Histopathological changes in the liver and gills were analyzed as biomarkers of general fish health. Only the concentrations of Cd, Na, and P in the muscles differed significantly. The OCPs and PCBs concentrations were below the detection limits, so fish meat consumption does not pose a risk concerning these substances. Hg and Cd exceeded the maximum allowed concentrations in some 4+ individuals, probably due to biomagnification. Gills were the most affected by metal exposure in both age classes. Histopathological changes and indices were minor and did not differ significantly between age classes, suggesting that pollution did not affect the morphology and structure of gills and liver. There were no significant correlations between elemental accumulation and fish condition or between histopathological scores. Therefore, both age classes can be used as bioindicators of pollution.",
journal = "Sustainability, Sustainability",
title = "Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution",
pages = "13-1",
number = "14",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6415"
}

Nikolić, D., Poleksić, V., Tasić, A., Smederevac-Lalić, M., Djikanović, V.,& Rašković, B.. (2023). Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution. in Sustainability, 15(14), 1-13.
https://hdl.handle.net/21.15107/rcub_agrospace_6415

Nikolić D, Poleksić V, Tasić A, Smederevac-Lalić M, Djikanović V, Rašković B. Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution. in Sustainability. 2023;15(14):1-13.
https://hdl.handle.net/21.15107/rcub_agrospace_6415 .

Nikolić, Dušan, Poleksić, Vesna, Tasić, Aleksandra, Smederevac-Lalić, Marija, Djikanović, Vesna, Rašković, Božidar, "Two Age Groups of Adult Pikeperch ( Sander lucioperca ) as Bioindicators of Aquatic Pollution" in Sustainability, 15, no. 14 (2023):1-13,
https://hdl.handle.net/21.15107/rcub_agrospace_6415 .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Poleksić, Vesna
AU  - Rašković, Božidar
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5859
AB  - Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garaši reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (IGI) and HP index of gills (IG), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garaši reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Science and Pollution Research
T1  - Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment
DO  - 10.1007/s11356-021-14526-w
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Poleksić, Vesna and Rašković, Božidar",
year = "2021",
abstract = "Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garaši reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (IGI) and HP index of gills (IG), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garaši reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Science and Pollution Research",
title = "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment",
doi = "10.1007/s11356-021-14526-w"
}

Nikolić, D., Skorić, S., Poleksić, V.,& Rašković, B.. (2021). Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s11356-021-14526-w

Nikolić D, Skorić S, Poleksić V, Rašković B. Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research. 2021;.
doi:10.1007/s11356-021-14526-w .

Nikolić, Dušan, Skorić, Stefan, Poleksić, Vesna, Rašković, Božidar, "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia) with human health risk assessment" in Environmental Science and Pollution Research (2021),
https://doi.org/10.1007/s11356-021-14526-w . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Nikolić, Dušan
AU  - Delibašić, Goran
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5821
AB  - A four-year survey (2012–2015) was carried out to examine the genetic diversity of cucumber mosaic virus (CMV) isolates infecting tomato, as well as the presence and diversity of their satellite RNAs (satRNAs), collecting a total of 226 samples throughout the most important growing regions in Serbia. Besides CMV-like symptoms, the collected samples also exhibited more severe symptoms, such as systemic necrosis of leaves, branches and stems, accompanied by fruit malformation and necrosis. In a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the presence of CMV was detected in approximately one quarter of the tested samples. Identification of CMV was confirmed by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the coat protein (CP) gene of a group of 11 selected Serbian CMV isolates. Phylogenetic analysis of the CMV CP sequences of these selected isolates revealed their heterogeneity, as they fell into two different subgroups, IA and II. An additional RT-PCR analysis of CMV positive isolates using satellite RNAs specific primers detected the presence of satRNAs in eight samples. Sequence and phylogenetic analyses showed that Serbian CMV satRNAs variants were very heterogeneous, belonging to necrogenic and non-necrogenic variants. Necrogenic variants were divided into two groups, B and B1, containing a characteristic ‘necrogenic consensus’ sequence at the 3′ end of the RNA. A necrotic phenotype co-determined by satRNAs was expressed in inoculated tomato plants.
PB  - Springer Science and Business Media B.V.
T2  - Eur J Plant Pathol
T1  - Characterization of cucumber mosaic virus and its satellite RNAs associated with tomato lethal necrosis in Serbia
DO  - 10.1007/s10658-021-02241-8
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Nikolić, Dušan and Delibašić, Goran",
year = "2021",
abstract = "A four-year survey (2012–2015) was carried out to examine the genetic diversity of cucumber mosaic virus (CMV) isolates infecting tomato, as well as the presence and diversity of their satellite RNAs (satRNAs), collecting a total of 226 samples throughout the most important growing regions in Serbia. Besides CMV-like symptoms, the collected samples also exhibited more severe symptoms, such as systemic necrosis of leaves, branches and stems, accompanied by fruit malformation and necrosis. In a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the presence of CMV was detected in approximately one quarter of the tested samples. Identification of CMV was confirmed by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the coat protein (CP) gene of a group of 11 selected Serbian CMV isolates. Phylogenetic analysis of the CMV CP sequences of these selected isolates revealed their heterogeneity, as they fell into two different subgroups, IA and II. An additional RT-PCR analysis of CMV positive isolates using satellite RNAs specific primers detected the presence of satRNAs in eight samples. Sequence and phylogenetic analyses showed that Serbian CMV satRNAs variants were very heterogeneous, belonging to necrogenic and non-necrogenic variants. Necrogenic variants were divided into two groups, B and B1, containing a characteristic ‘necrogenic consensus’ sequence at the 3′ end of the RNA. A necrotic phenotype co-determined by satRNAs was expressed in inoculated tomato plants.",
publisher = "Springer Science and Business Media B.V.",
journal = "Eur J Plant Pathol",
title = "Characterization of cucumber mosaic virus and its satellite RNAs associated with tomato lethal necrosis in Serbia",
doi = "10.1007/s10658-021-02241-8"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Nikolić, D.,& Delibašić, G.. (2021). Characterization of cucumber mosaic virus and its satellite RNAs associated with tomato lethal necrosis in Serbia. in Eur J Plant Pathol
Springer Science and Business Media B.V...
https://doi.org/10.1007/s10658-021-02241-8

Stanković I, Vučurović A, Zečević K, Petrović B, Nikolić D, Delibašić G. Characterization of cucumber mosaic virus and its satellite RNAs associated with tomato lethal necrosis in Serbia. in Eur J Plant Pathol. 2021;.
doi:10.1007/s10658-021-02241-8 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Nikolić, Dušan, Delibašić, Goran, "Characterization of cucumber mosaic virus and its satellite RNAs associated with tomato lethal necrosis in Serbia" in Eur J Plant Pathol (2021),
https://doi.org/10.1007/s10658-021-02241-8 . .

TY  - DATA
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Nikolić, Dušan
AU  - Delibašić, Goran
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5994
PB  - Springer Science and Business Media B.V.
T2  - Eur J Plant Pathol
T1  - Supplementary data for the article: Stanković, I.; Vučurović, A.; Zečević, K.; Petrović, B.; Nikolić, D.; Delibašić, G. Characterization of Cucumber Mosaic Virus and Its Satellite RNAs Associated with Tomato Lethal Necrosis in Serbia. Eur J Plant Pathol 2021. https://doi.org/10.1007/s10658-021-02241-8.
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5994
ER  - 

@misc{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Nikolić, Dušan and Delibašić, Goran",
year = "2021",
publisher = "Springer Science and Business Media B.V.",
journal = "Eur J Plant Pathol",
title = "Supplementary data for the article: Stanković, I.; Vučurović, A.; Zečević, K.; Petrović, B.; Nikolić, D.; Delibašić, G. Characterization of Cucumber Mosaic Virus and Its Satellite RNAs Associated with Tomato Lethal Necrosis in Serbia. Eur J Plant Pathol 2021. https://doi.org/10.1007/s10658-021-02241-8.",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5994"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Nikolić, D.,& Delibašić, G.. (2021). Supplementary data for the article: Stanković, I.; Vučurović, A.; Zečević, K.; Petrović, B.; Nikolić, D.; Delibašić, G. Characterization of Cucumber Mosaic Virus and Its Satellite RNAs Associated with Tomato Lethal Necrosis in Serbia. Eur J Plant Pathol 2021. https://doi.org/10.1007/s10658-021-02241-8.. in Eur J Plant Pathol
Springer Science and Business Media B.V...
https://hdl.handle.net/21.15107/rcub_agrospace_5994

Stanković I, Vučurović A, Zečević K, Petrović B, Nikolić D, Delibašić G. Supplementary data for the article: Stanković, I.; Vučurović, A.; Zečević, K.; Petrović, B.; Nikolić, D.; Delibašić, G. Characterization of Cucumber Mosaic Virus and Its Satellite RNAs Associated with Tomato Lethal Necrosis in Serbia. Eur J Plant Pathol 2021. https://doi.org/10.1007/s10658-021-02241-8.. in Eur J Plant Pathol. 2021;.
https://hdl.handle.net/21.15107/rcub_agrospace_5994 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Nikolić, Dušan, Delibašić, Goran, "Supplementary data for the article: Stanković, I.; Vučurović, A.; Zečević, K.; Petrović, B.; Nikolić, D.; Delibašić, G. Characterization of Cucumber Mosaic Virus and Its Satellite RNAs Associated with Tomato Lethal Necrosis in Serbia. Eur J Plant Pathol 2021. https://doi.org/10.1007/s10658-021-02241-8." in Eur J Plant Pathol (2021),
https://hdl.handle.net/21.15107/rcub_agrospace_5994 .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Rašković, Božidar
AU  - Lenhardt, Mirjana
AU  - Krpo-Cetković, Jasmina
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5326
AB  - This study was conducted on individuals of European perch from six reservoirs in Serbia with different properties, used for different purposes, and situated at different altitudes. Samples of muscle, gills, and liver, as well as water samples, were used for analysis of elemental accumulation. Histopathological (HP) analysis of gills and liver, and Fulton's condition factor (CF) were used as biomarkers. Analysis of Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was conducted by inductively-coupled plasma optical emission spectrometry (ICP-OES), and accordingly, metal pollution index and bioaccumulation factor (BAF) were calculated. Elemental accumulation varied considerably both among tissues and reservoirs. Reservoirs located on large low-altitude rivers were the most heavily polluted with metals (Meduvrgje, on the Zapadna Morava River, and Lake Sava, on the Sava River). Of three tissues analyzed, the gills were most affected by pollution. The levels of Hg in muscle decreased with an increase in reservoir altitude. Specimens sampled from the highest altitude reservoir were the least affected by HP alterations; however, HP differences between the reservoirs were not statistically significant, and the severity of HP changes was generally low. No significant correlations between the elemental accumulation or HP indices with CF were observed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)
VL  - 244
DO  - 10.1016/j.chemosphere.2019.125503
ER  - 

@article{
author = "Nikolić, Dušan and Skorić, Stefan and Rašković, Božidar and Lenhardt, Mirjana and Krpo-Cetković, Jasmina",
year = "2020",
abstract = "This study was conducted on individuals of European perch from six reservoirs in Serbia with different properties, used for different purposes, and situated at different altitudes. Samples of muscle, gills, and liver, as well as water samples, were used for analysis of elemental accumulation. Histopathological (HP) analysis of gills and liver, and Fulton's condition factor (CF) were used as biomarkers. Analysis of Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn was conducted by inductively-coupled plasma optical emission spectrometry (ICP-OES), and accordingly, metal pollution index and bioaccumulation factor (BAF) were calculated. Elemental accumulation varied considerably both among tissues and reservoirs. Reservoirs located on large low-altitude rivers were the most heavily polluted with metals (Meduvrgje, on the Zapadna Morava River, and Lake Sava, on the Sava River). Of three tissues analyzed, the gills were most affected by pollution. The levels of Hg in muscle decreased with an increase in reservoir altitude. Specimens sampled from the highest altitude reservoir were the least affected by HP alterations; however, HP differences between the reservoirs were not statistically significant, and the severity of HP changes was generally low. No significant correlations between the elemental accumulation or HP indices with CF were observed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)",
volume = "244",
doi = "10.1016/j.chemosphere.2019.125503"
}

Nikolić, D., Skorić, S., Rašković, B., Lenhardt, M.,& Krpo-Cetković, J.. (2020). Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis). in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 244.
https://doi.org/10.1016/j.chemosphere.2019.125503

Nikolić D, Skorić S, Rašković B, Lenhardt M, Krpo-Cetković J. Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis). in Chemosphere. 2020;244.
doi:10.1016/j.chemosphere.2019.125503 .

Nikolić, Dušan, Skorić, Stefan, Rašković, Božidar, Lenhardt, Mirjana, Krpo-Cetković, Jasmina, "Impact of reservoir properties on elemental accumulation and histopathology of European perch (Perca fluviatilis)" in Chemosphere, 244 (2020),
https://doi.org/10.1016/j.chemosphere.2019.125503 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Petrović-Obradović, Olivera
AU  - Radonjić, Andja
AU  - Nikolić, Dušan
AU  - Zečević, Katarina
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4890
AB  - The fauna of aphids appearing on cucurbits, including oilseed pumpkin have been poorly investigated in Serbia. Yellow water traps were used to determine the diversity of aphid species visiting cucurbits in Serbia and to monitor their flight activity. During the years 2009 to 2011, a total of 1,447 specimens were collected and a total of 57 different aphid taxa were identified. In 2009, the highest total number of aphids were caught (755), followed by 203 and 489 aphids in 2010 and 2011, respectively. The most abundant species were Aphis fabae (15%), Myizus persicae (13.4%) and Acyrthosiphonpisum (11.7%), followed by Anoecia corni (6.4%) and Aphis gossypii (5.6%). All of them, except A. corni, are reported as efficient vectors of cucurbit viruses. These five species represent 52.1% of all aphid species collected within this study. Out of 57 taxa found in oilseed pumpkin crops, 17 are known as vectors of ZYMV, 18 of WMV and 15 of CMV. Generally, 25 out of a total of 57 taxa are known as vectors of at least one of three viruses present in Serbia. From total of 1,447 specimens collected during three years of investigation, 917 (63.4%) are vectors of at least one of three viruses. Individual oilseed pumpkin plants were frequently colonized by A. gossypii during all three years of investigation. Results of this study showed that Serbian oilseed pumpkin growing localities are grouped among medium to reach in aphidofauna, according to total Shannon-Weaver index values which varied from 1.8 to 2.8. The highest value of Shannon-Weaver (2.8) was recorded in the Bački Petrovac I locality in 2009, while the lowest Shannon-Weaver (1.8) was recorded in Kisač locality in 2010. The maximum values of Shannon-Weaver diversity index in almost all crops were recorded in the second week of investigation or in early stages of oilseed pumpkin growth when plants had 5-6 true leaves or were in flowering stage, when potential virus infection could lead to significant yield losses.
AB  - Afidofauna koja se javlja na tikvama, uključujući uljanu tikvu do sada nije dobro ispitana u Srbiji. Da bi utvrdili diverzitet lisnih vaši koje posećuju useve tikava u Srbiji, kao i da bi pratili aktivnost i dinamiku njihovog leta, koristili smo žute lovne klopke. Tokom perioda od 2009. do 2011. godine ukupno je sakupljeno 1447 jedinki i determinisano 57 taksona lisnih vaši. Tokom 2009. godine, uhvaćen je najveći broj jedinki (755), dok je tokom 2010. i 2011. godine uhvaćeno 203, odnosno 489 jedinki lisnih vaši. Najzastupljenije vrste bile su Aphis fabae (15%), Myzus persicae (13,4%) i Acyrthosiphon pisum (11,7%), a zatim Anoecia corni (6,4%) i Aphis gossypii (5,6%). Za sve vrste, osim za A. corni, potvrđeno je da su vektori virusa tikava. Ovih pet najzastupljenijih vrsta predstavljaju 52,1% od svih sakupljenih jedinki vaši u toku ovih istraživanja. Od ukupno 57 taksona nađenih u usevima uljane tikve, 17 su poznati vektori ZYMV, 18 WMV i 15 CMV. Ukupno posmatrano, 25 od 57 nađenih taksona su vektori bar jednog od tri virusa tikava prisutnih u Srbiji. Od ukupno 1447 jedinki sakupljenih tokom tri godine istraživanja, 917 (63,4%) su vektori bar jednog od tri virusa tikava. Kolonizacija pojedinačnih biljaka uljane tikve vrstom A. gossypii uočavana je često tokom sve tri godine istraživanja. Rezultati ovih istraživanja, na osnovu ukupnog Shannon-Weaver-ovog indeksa diverziteta koji je varirao između 1,8 i 2,8, ukazuju da se lokaliteti na kojima se gaji uljana tikva u Srbiji svrstavaju u red srednje bogatih do bogatih po sastavu i brojnosti afidofaune. Najveća vrednost Shannon-Weaver-ovog indeksa diverziteta (2,8) zabeležena je tokom 2009. godine na lokalitetu Bački Petrovac I, dok je najniži indeks diverziteta (1,8) zabeležen na lokalitetu Kisač tokom 2010. godine. Shanon-Weaver-ov indeks diverziteta u gotovo svim ispitivanim usevima dostigao je maksimalne vrednosti u drugoj nedelji istraživanja ili u ranim fazama razvoja uljane tikve kada biljke imaju 5-6 pravih listova ili su u fazi cvetanja, što predstavlja najosetljivije faze za ostvarenje infekcije virusima koje mogu da dovedu do značajnih gubitaka u prinosu.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia
T1  - Diverzitet i praćenje leta lisnih vaši kao potencijalnih vektora virusa uljane tikve u Srbiji
EP  - 79
IS  - 2
SP  - 72
VL  - 55
DO  - 10.5937/ratpov55-16608
ER  - 

@article{
author = "Vučurović, Ana and Petrović-Obradović, Olivera and Radonjić, Andja and Nikolić, Dušan and Zečević, Katarina and Stanković, Ivana and Krstić, Branka",
year = "2018",
abstract = "The fauna of aphids appearing on cucurbits, including oilseed pumpkin have been poorly investigated in Serbia. Yellow water traps were used to determine the diversity of aphid species visiting cucurbits in Serbia and to monitor their flight activity. During the years 2009 to 2011, a total of 1,447 specimens were collected and a total of 57 different aphid taxa were identified. In 2009, the highest total number of aphids were caught (755), followed by 203 and 489 aphids in 2010 and 2011, respectively. The most abundant species were Aphis fabae (15%), Myizus persicae (13.4%) and Acyrthosiphonpisum (11.7%), followed by Anoecia corni (6.4%) and Aphis gossypii (5.6%). All of them, except A. corni, are reported as efficient vectors of cucurbit viruses. These five species represent 52.1% of all aphid species collected within this study. Out of 57 taxa found in oilseed pumpkin crops, 17 are known as vectors of ZYMV, 18 of WMV and 15 of CMV. Generally, 25 out of a total of 57 taxa are known as vectors of at least one of three viruses present in Serbia. From total of 1,447 specimens collected during three years of investigation, 917 (63.4%) are vectors of at least one of three viruses. Individual oilseed pumpkin plants were frequently colonized by A. gossypii during all three years of investigation. Results of this study showed that Serbian oilseed pumpkin growing localities are grouped among medium to reach in aphidofauna, according to total Shannon-Weaver index values which varied from 1.8 to 2.8. The highest value of Shannon-Weaver (2.8) was recorded in the Bački Petrovac I locality in 2009, while the lowest Shannon-Weaver (1.8) was recorded in Kisač locality in 2010. The maximum values of Shannon-Weaver diversity index in almost all crops were recorded in the second week of investigation or in early stages of oilseed pumpkin growth when plants had 5-6 true leaves or were in flowering stage, when potential virus infection could lead to significant yield losses., Afidofauna koja se javlja na tikvama, uključujući uljanu tikvu do sada nije dobro ispitana u Srbiji. Da bi utvrdili diverzitet lisnih vaši koje posećuju useve tikava u Srbiji, kao i da bi pratili aktivnost i dinamiku njihovog leta, koristili smo žute lovne klopke. Tokom perioda od 2009. do 2011. godine ukupno je sakupljeno 1447 jedinki i determinisano 57 taksona lisnih vaši. Tokom 2009. godine, uhvaćen je najveći broj jedinki (755), dok je tokom 2010. i 2011. godine uhvaćeno 203, odnosno 489 jedinki lisnih vaši. Najzastupljenije vrste bile su Aphis fabae (15%), Myzus persicae (13,4%) i Acyrthosiphon pisum (11,7%), a zatim Anoecia corni (6,4%) i Aphis gossypii (5,6%). Za sve vrste, osim za A. corni, potvrđeno je da su vektori virusa tikava. Ovih pet najzastupljenijih vrsta predstavljaju 52,1% od svih sakupljenih jedinki vaši u toku ovih istraživanja. Od ukupno 57 taksona nađenih u usevima uljane tikve, 17 su poznati vektori ZYMV, 18 WMV i 15 CMV. Ukupno posmatrano, 25 od 57 nađenih taksona su vektori bar jednog od tri virusa tikava prisutnih u Srbiji. Od ukupno 1447 jedinki sakupljenih tokom tri godine istraživanja, 917 (63,4%) su vektori bar jednog od tri virusa tikava. Kolonizacija pojedinačnih biljaka uljane tikve vrstom A. gossypii uočavana je često tokom sve tri godine istraživanja. Rezultati ovih istraživanja, na osnovu ukupnog Shannon-Weaver-ovog indeksa diverziteta koji je varirao između 1,8 i 2,8, ukazuju da se lokaliteti na kojima se gaji uljana tikva u Srbiji svrstavaju u red srednje bogatih do bogatih po sastavu i brojnosti afidofaune. Najveća vrednost Shannon-Weaver-ovog indeksa diverziteta (2,8) zabeležena je tokom 2009. godine na lokalitetu Bački Petrovac I, dok je najniži indeks diverziteta (1,8) zabeležen na lokalitetu Kisač tokom 2010. godine. Shanon-Weaver-ov indeks diverziteta u gotovo svim ispitivanim usevima dostigao je maksimalne vrednosti u drugoj nedelji istraživanja ili u ranim fazama razvoja uljane tikve kada biljke imaju 5-6 pravih listova ili su u fazi cvetanja, što predstavlja najosetljivije faze za ostvarenje infekcije virusima koje mogu da dovedu do značajnih gubitaka u prinosu.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia, Diverzitet i praćenje leta lisnih vaši kao potencijalnih vektora virusa uljane tikve u Srbiji",
pages = "79-72",
number = "2",
volume = "55",
doi = "10.5937/ratpov55-16608"
}

Vučurović, A., Petrović-Obradović, O., Radonjić, A., Nikolić, D., Zečević, K., Stanković, I.,& Krstić, B.. (2018). Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 55(2), 72-79.
https://doi.org/10.5937/ratpov55-16608

Vučurović A, Petrović-Obradović O, Radonjić A, Nikolić D, Zečević K, Stanković I, Krstić B. Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia. in Ratarstvo i povrtarstvo. 2018;55(2):72-79.
doi:10.5937/ratpov55-16608 .

Vučurović, Ana, Petrović-Obradović, Olivera, Radonjić, Andja, Nikolić, Dušan, Zečević, Katarina, Stanković, Ivana, Krstić, Branka, "Diversity and flight activity of aphid species as potential vectors of oilseed pumpkin viruses in Serbia" in Ratarstvo i povrtarstvo, 55, no. 2 (2018):72-79,
https://doi.org/10.5937/ratpov55-16608 . .

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Radović, Nikola
AU  - Zečević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4649
AB  - In a two-year survey (2011-2012), 3220 samples were collected and analyzed in order to determine the presence and distribution of viruses in tomato crops at 56 localities of 18 districts in Serbia. Out of 12 viruses tested, Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) were detected in 42.1, 40, 11, 8.6, 2.3 and 1.3% of the total tested samples, respectively. The results revealed that CMV was prevalent in 2011 and PVY in 2012. CMV and PVY, apart from being predominant, were also the most widespread viruses. In general, single infections were the most frequent type of infection. Additionally, the most common mixed infections were double infections and the most prevalent combination was CMV and PVY. In 2011, the incidence of diseases and the percentage of all infection types were significantly higher than in 2012. Furthermore, in 2011, regardless of total single infections being prevalent compared to mixed infections, two prevailing viruses were commonly detected in mixed infections. The additional molecular testing of ELISA-negative samples using virus specific primers did not reveal the presence of Pepino mosaic virus (PepMV), Tomato yellow leaf curl virus (TYLC), Tomato infections chlorosis virus (TICV) and Tomato chlorosis virus (ToCV).
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Viruses affecting tomato crops in Serbia
EP  - 235
IS  - 1
SP  - 225
VL  - 152
DO  - 10.1007/s10658-018-1467-y
ER  - 

@article{
author = "Nikolić, Dušan and Vučurović, Ana and Stanković, Ivana and Radović, Nikola and Zečević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2018",
abstract = "In a two-year survey (2011-2012), 3220 samples were collected and analyzed in order to determine the presence and distribution of viruses in tomato crops at 56 localities of 18 districts in Serbia. Out of 12 viruses tested, Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) were detected in 42.1, 40, 11, 8.6, 2.3 and 1.3% of the total tested samples, respectively. The results revealed that CMV was prevalent in 2011 and PVY in 2012. CMV and PVY, apart from being predominant, were also the most widespread viruses. In general, single infections were the most frequent type of infection. Additionally, the most common mixed infections were double infections and the most prevalent combination was CMV and PVY. In 2011, the incidence of diseases and the percentage of all infection types were significantly higher than in 2012. Furthermore, in 2011, regardless of total single infections being prevalent compared to mixed infections, two prevailing viruses were commonly detected in mixed infections. The additional molecular testing of ELISA-negative samples using virus specific primers did not reveal the presence of Pepino mosaic virus (PepMV), Tomato yellow leaf curl virus (TYLC), Tomato infections chlorosis virus (TICV) and Tomato chlorosis virus (ToCV).",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Viruses affecting tomato crops in Serbia",
pages = "235-225",
number = "1",
volume = "152",
doi = "10.1007/s10658-018-1467-y"
}

Nikolić, D., Vučurović, A., Stanković, I., Radović, N., Zečević, K., Bulajić, A.,& Krstić, B.. (2018). Viruses affecting tomato crops in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 152(1), 225-235.
https://doi.org/10.1007/s10658-018-1467-y

Nikolić D, Vučurović A, Stanković I, Radović N, Zečević K, Bulajić A, Krstić B. Viruses affecting tomato crops in Serbia. in European Journal of Plant Pathology. 2018;152(1):225-235.
doi:10.1007/s10658-018-1467-y .

Nikolić, Dušan, Vučurović, Ana, Stanković, Ivana, Radović, Nikola, Zečević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "Viruses affecting tomato crops in Serbia" in European Journal of Plant Pathology, 152, no. 1 (2018):225-235,
https://doi.org/10.1007/s10658-018-1467-y . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Nikolić, Dušan
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Krstić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4547
AB  - So far, tomato is a natural host for about 146 plant viruses, but several of them have caused a considerable economic damage constantly, such as aphid-borne viruses, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), the thrips-transmitted Tomato spotted wilt orthotospovirus (TSWV) and mechanically- and seed- transmitted Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV). However, in recent years emerging viruses including Pepino mosaic virus (PepMV) as well as whitefly-transmited viruses: Tomato yellow leaf curl virus (TYLCV), Tomato chlorosis virus (ToCV), Tomato infectious chlorosis virus (TICV) and Tomato torrado virus (ToTV) have appeared in tomato crops posing a serious threat to its production in many European countries. These viruses with a rapidly increasing incidence are considered extremely dangerous and their occurrence can be expected in our country. If the eradication of these viruses has failed or if this is not possible, other strategies are needed. In this article, specific measures for the prevention and control of these viruses are discussed.
AB  - Paradajz je domaćin oko 146 virusa, međutim samo nekoliko njih izaziva značajne ekonomske štete u kontinuitetu uključujući virus mozaika krastavca (Cucumber mosaic virus, CMV), Y virus krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus mozaika duvana (Tobacco mosaic virus, TMV) i virus mozaika paradajza (Tomato mosaic virus, ToMV). Međutim, poslednjih godina značajne štete u proizvodnji paradajza u mnogim evropskih zemljama prouzrokuju 'emerging' virusi, koji se smatraju ekstremno opasnim i čija se pojava očekuje i kod nas: virus mozaika pepina (Pepino mosaic virus, PepMV) i grupa virusa koji se prenose leptirastim vašima, uključujući virus žute uvijenosti lista paradajza (Tomato yellow leaf curl virus, TYLCV), virus hloroze paradajza (Tomato chlorosis virus, ToCV), virus infektivne hloroze paradajza (Tomato infectious chlorosis virus, TICV) i torado virus paradajza (Tomato torrado virus, ToTV). Ukoliko eradikacija ovih virusa ne uspe ili nije moguća, potrebno je sprovesti druge mere za prevenciju i kontrolu oboljenja koje ovi virusi izazivaju, a koje su prodiskutovane u ovom radu.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Viruses of tomato with special emphasis on emerging viruses
T1  - Virusi paradajza sa posebnim osvrtom na 'emerging' viruse
EP  - 646
IS  - 6
SP  - 628
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4547
ER  - 

@article{
author = "Stanković, Ivana and Nikolić, Dušan and Vučurović, Ana and Zečević, Katarina and Krstić, Branka",
year = "2017",
abstract = "So far, tomato is a natural host for about 146 plant viruses, but several of them have caused a considerable economic damage constantly, such as aphid-borne viruses, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), the thrips-transmitted Tomato spotted wilt orthotospovirus (TSWV) and mechanically- and seed- transmitted Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV). However, in recent years emerging viruses including Pepino mosaic virus (PepMV) as well as whitefly-transmited viruses: Tomato yellow leaf curl virus (TYLCV), Tomato chlorosis virus (ToCV), Tomato infectious chlorosis virus (TICV) and Tomato torrado virus (ToTV) have appeared in tomato crops posing a serious threat to its production in many European countries. These viruses with a rapidly increasing incidence are considered extremely dangerous and their occurrence can be expected in our country. If the eradication of these viruses has failed or if this is not possible, other strategies are needed. In this article, specific measures for the prevention and control of these viruses are discussed., Paradajz je domaćin oko 146 virusa, međutim samo nekoliko njih izaziva značajne ekonomske štete u kontinuitetu uključujući virus mozaika krastavca (Cucumber mosaic virus, CMV), Y virus krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus mozaika duvana (Tobacco mosaic virus, TMV) i virus mozaika paradajza (Tomato mosaic virus, ToMV). Međutim, poslednjih godina značajne štete u proizvodnji paradajza u mnogim evropskih zemljama prouzrokuju 'emerging' virusi, koji se smatraju ekstremno opasnim i čija se pojava očekuje i kod nas: virus mozaika pepina (Pepino mosaic virus, PepMV) i grupa virusa koji se prenose leptirastim vašima, uključujući virus žute uvijenosti lista paradajza (Tomato yellow leaf curl virus, TYLCV), virus hloroze paradajza (Tomato chlorosis virus, ToCV), virus infektivne hloroze paradajza (Tomato infectious chlorosis virus, TICV) i torado virus paradajza (Tomato torrado virus, ToTV). Ukoliko eradikacija ovih virusa ne uspe ili nije moguća, potrebno je sprovesti druge mere za prevenciju i kontrolu oboljenja koje ovi virusi izazivaju, a koje su prodiskutovane u ovom radu.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Viruses of tomato with special emphasis on emerging viruses, Virusi paradajza sa posebnim osvrtom na 'emerging' viruse",
pages = "646-628",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4547"
}

Stanković, I., Nikolić, D., Vučurović, A., Zečević, K.,& Krstić, B.. (2017). Viruses of tomato with special emphasis on emerging viruses. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 628-646.
https://hdl.handle.net/21.15107/rcub_agrospace_4547

Stanković I, Nikolić D, Vučurović A, Zečević K, Krstić B. Viruses of tomato with special emphasis on emerging viruses. in Biljni lekar. 2017;45(6):628-646.
https://hdl.handle.net/21.15107/rcub_agrospace_4547 .

Stanković, Ivana, Nikolić, Dušan, Vučurović, Ana, Zečević, Katarina, Krstić, Branka, "Viruses of tomato with special emphasis on emerging viruses" in Biljni lekar, 45, no. 6 (2017):628-646,
https://hdl.handle.net/21.15107/rcub_agrospace_4547 .

TY  - JOUR
AU  - Vučurović, Ivan
AU  - Nikolić, Dušan
AU  - Radović, Nikola
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4603
AB  - Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.
AB  - Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Incidence and distribution of leek yellow stripe virus in allium crops in Serbia
T1  - Prisustvo i rasprostranjenost virusa žute prugavosti praziluka u usevima različitih vrsta lukova u Srbiji
EP  - 155
IS  - 3-4
SP  - 145
VL  - 32
DO  - 10.2298/PIF1704145V
ER  - 

@article{
author = "Vučurović, Ivan and Nikolić, Dušan and Radović, Nikola and Vučurović, Ana and Ristić, Danijela and Krstić, Branka and Stanković, Ivana",
year = "2017",
abstract = "Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E., Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia, Prisustvo i rasprostranjenost virusa žute prugavosti praziluka u usevima različitih vrsta lukova u Srbiji",
pages = "155-145",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704145V"
}

Vučurović, I., Nikolić, D., Radović, N., Vučurović, A., Ristić, D., Krstić, B.,& Stanković, I.. (2017). Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 32(3-4), 145-155.
https://doi.org/10.2298/PIF1704145V

Vučurović I, Nikolić D, Radović N, Vučurović A, Ristić D, Krstić B, Stanković I. Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina. 2017;32(3-4):145-155.
doi:10.2298/PIF1704145V .

Vučurović, Ivan, Nikolić, Dušan, Radović, Nikola, Vučurović, Ana, Ristić, Danijela, Krstić, Branka, Stanković, Ivana, "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):145-155,
https://doi.org/10.2298/PIF1704145V . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Milojević, Katarina
AU  - Vučurović, Ana
AU  - Nikolić, Dušan
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3615
AB  - In the period 2005-2014, total of 19 isolates of Alternaria petroselini originating from parsley leaves, taproot and seed, as well as infested soil were collected and analyzed on the bases of morphological, pathogenic and molecular features. All isolates formed dark gray colonies on PDA and subsurfacely produced microsclerotia, as well as pigmented, broadly ellipsoidal, singly feodyctiosporic conidia. Pathogenicity and host range studies using spore suspension spray inoculation, revealed that isolates were pathogenic for parsley, parsnip, celery and coriander, weakly pathogenic for carrot, fennel and anise, and not pathogenic for onion, cabbage, pepper, tomato, and cucumber. Molecular detection utilizing A. radicina specific primers failed to demonstrate difference between two closely related but diffe­rent species, A. radicina and A. petroselini. For further confirmation of idendty of Serbian A. petroselini isolates, sequencing of selected genomic fragments including ITS rDNA, Alt a1 and EF1a protein coding genes was performed. BLAST search revealed that the most reliable molecular delineation between A. petroselini and closely related species could be achieved on the bases of sequence analysis of Alt a1 and EF1a genes (25-27, and 10-12 nucleotide differences from A. radicina isolates, respectively), while sequence of ITS rDNA were helpful, but not completely informative for identification of A. petroselini isolates.
AB  - U periodu od 2005. do 2014. godine, sakupljeno je 19 izolata Alternaria petroselini poreklom iz lista, korena i semena peršuna, kao i infestiranog zemljišta i analizirano na osnovu morfoloških, patogenih i molekularnih osobina. Svi izolati formirali su tamnosive kolonije na PDA i supstratno obrazovali mikrosklerocije, kao i obojene, elipsoidno okruglaste, feodiktiosporne konidije pojedinačno na kratkim konidioforama. Ispitujući patogenost i krug domaćina inokulacijom suspenzijom spora, svi ispitivani izolati ispoljili su patogenost za peršun, paštrnak, celer i korijander, slabu patogenost za mrkvu, morač i anis, a nisu bili infektivni za crni luk, kupus, papriku, paradajz i krastavac. Molekularna detekcija korišćenjem prajmera specifičnih za detekciju A. radicina nije uspela da napravi razliku između izolata dve srodne, ali različite vrste, A. radicina i A. petroselini. Dalja karakterizacija izolata A. petroselini poreklom iz Srbije, obavljena je sekvenciranjem različitih genskih segmenata uključujući ITS rDNK, kao i Alt a1 i EF1a gene. BLAST analiza pokazala je da su sekvence Alt a1 i EF1a gena najpouzdanije za razlikovanje A. petroselini od drugih blisko srodnih vrsta (25-27 odnosno 10-12 nukleotida razlike od izolata A. radicina). Sekvence ITS rDNK regiona bile su korisne, ali ne i potpuno informativne za identifikaciju izolata A. petroselini.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Black leaf spot: Important disease of parsley in Serbia
T1  - Pegavost i sušenje lista - značajna bolest peršuna u Srbiji
EP  - 154
IS  - 4
SP  - 146
VL  - 65
DO  - 10.5937/zasbilj1404146S
ER  - 

@article{
author = "Stanković, Ivana and Milojević, Katarina and Vučurović, Ana and Nikolić, Dušan and Krstić, Branka and Bulajić, Aleksandra",
year = "2014",
abstract = "In the period 2005-2014, total of 19 isolates of Alternaria petroselini originating from parsley leaves, taproot and seed, as well as infested soil were collected and analyzed on the bases of morphological, pathogenic and molecular features. All isolates formed dark gray colonies on PDA and subsurfacely produced microsclerotia, as well as pigmented, broadly ellipsoidal, singly feodyctiosporic conidia. Pathogenicity and host range studies using spore suspension spray inoculation, revealed that isolates were pathogenic for parsley, parsnip, celery and coriander, weakly pathogenic for carrot, fennel and anise, and not pathogenic for onion, cabbage, pepper, tomato, and cucumber. Molecular detection utilizing A. radicina specific primers failed to demonstrate difference between two closely related but diffe­rent species, A. radicina and A. petroselini. For further confirmation of idendty of Serbian A. petroselini isolates, sequencing of selected genomic fragments including ITS rDNA, Alt a1 and EF1a protein coding genes was performed. BLAST search revealed that the most reliable molecular delineation between A. petroselini and closely related species could be achieved on the bases of sequence analysis of Alt a1 and EF1a genes (25-27, and 10-12 nucleotide differences from A. radicina isolates, respectively), while sequence of ITS rDNA were helpful, but not completely informative for identification of A. petroselini isolates., U periodu od 2005. do 2014. godine, sakupljeno je 19 izolata Alternaria petroselini poreklom iz lista, korena i semena peršuna, kao i infestiranog zemljišta i analizirano na osnovu morfoloških, patogenih i molekularnih osobina. Svi izolati formirali su tamnosive kolonije na PDA i supstratno obrazovali mikrosklerocije, kao i obojene, elipsoidno okruglaste, feodiktiosporne konidije pojedinačno na kratkim konidioforama. Ispitujući patogenost i krug domaćina inokulacijom suspenzijom spora, svi ispitivani izolati ispoljili su patogenost za peršun, paštrnak, celer i korijander, slabu patogenost za mrkvu, morač i anis, a nisu bili infektivni za crni luk, kupus, papriku, paradajz i krastavac. Molekularna detekcija korišćenjem prajmera specifičnih za detekciju A. radicina nije uspela da napravi razliku između izolata dve srodne, ali različite vrste, A. radicina i A. petroselini. Dalja karakterizacija izolata A. petroselini poreklom iz Srbije, obavljena je sekvenciranjem različitih genskih segmenata uključujući ITS rDNK, kao i Alt a1 i EF1a gene. BLAST analiza pokazala je da su sekvence Alt a1 i EF1a gena najpouzdanije za razlikovanje A. petroselini od drugih blisko srodnih vrsta (25-27 odnosno 10-12 nukleotida razlike od izolata A. radicina). Sekvence ITS rDNK regiona bile su korisne, ali ne i potpuno informativne za identifikaciju izolata A. petroselini.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Black leaf spot: Important disease of parsley in Serbia, Pegavost i sušenje lista - značajna bolest peršuna u Srbiji",
pages = "154-146",
number = "4",
volume = "65",
doi = "10.5937/zasbilj1404146S"
}

Stanković, I., Milojević, K., Vučurović, A., Nikolić, D., Krstić, B.,& Bulajić, A.. (2014). Black leaf spot: Important disease of parsley in Serbia. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 65(4), 146-154.
https://doi.org/10.5937/zasbilj1404146S

Stanković I, Milojević K, Vučurović A, Nikolić D, Krstić B, Bulajić A. Black leaf spot: Important disease of parsley in Serbia. in Zaštita bilja. 2014;65(4):146-154.
doi:10.5937/zasbilj1404146S .

Stanković, Ivana, Milojević, Katarina, Vučurović, Ana, Nikolić, Dušan, Krstić, Branka, Bulajić, Aleksandra, "Black leaf spot: Important disease of parsley in Serbia" in Zaštita bilja, 65, no. 4 (2014):146-154,
https://doi.org/10.5937/zasbilj1404146S . .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Nikolić, Dušan
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3242
AB  - Investigation of the presence and distribution of Cucumber mosaic virus in watermelon crops in Serbia during 2012 revealed that the virus was present in five out of six surveyed localities. CMV was detected in 24.05% of total serologically tested samples using DAS-ELI­SA method and the virus was the most prevalent in the locality of Silbaš. During this investigation, Cucumber mosaic virus was present more often in mixed infections with ZYMV and/or WMV (12.66%), while the single infection was detected in lower percentage, 11.39%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and two of them were selected for further biological characterization. Concerning type and severity of symptoms on test plants, the investigated isolates exhibited different phenotypic features. Molecular detection was performed by amplification of a fragment of 871 bp in all tested isolates, using the primer pair CMVCPfwd/CPrev that amplify the entire coat protein gene and part of 5' and 3' UTR regions. The affiliation of Serbian CMV isolates from watermelon to subgroup IA, the group that includes most of the isolates selected for phylogenetic analysis, was determined by sequence analysis of the complete CP gene and reconstruction of phylogenetic tree.
AB  - Proučavanjem pojave i rasprostranjenosti virusa mozaika krastavca u usevu lubenice u Srbiji tokom 2012. godine, prisustvo virusa utvrđeno je u pet od šest pregledanih lokaliteta. Prisustvo virusa ustanovljeno je u 24,05% serološki testiranih uzoraka (DAS-ELISA), a najzastupljeniji je bio na lokalitetu Silbaš. Tokom ovih istraživanja virus mozaika krastavca češće je bio prisutan u mešanim infekcijama sa ZYMV i/ili WMV (12,66%), dok je prisustvo pojedinačne infekcije utvrđeno u nešto nižem procentu, 11,39%. Mehaničkim inokulacijama N. glutinosa, dobijeno je pet izolata, od kojih su dva odabrana za dalju biološku karakterizaciju. Na osnovu tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem para prajmera CMVCPfwd/CPrev, koji omogućavaju umnožavanje gena za protein omotača i deo 5' i 3' UTR regiona. Analizom sekvenci kompletnog gena za protein omotača i rekonstrukcijom filogenetskog stabla, utvrđena je pripadnost izolata CMV iz lubenice iz Srbije podgrupi IA, koja obuhvata većinu izolata odabranih za filogenetsku analizu.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia
T1  - Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji
EP  - 25
IS  - 1
SP  - 14
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3242
ER  - 

@article{
author = "Milojević, Katarina and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Nikolić, Dušan and Bulajić, Aleksandra and Krstić, Branka",
year = "2013",
abstract = "Investigation of the presence and distribution of Cucumber mosaic virus in watermelon crops in Serbia during 2012 revealed that the virus was present in five out of six surveyed localities. CMV was detected in 24.05% of total serologically tested samples using DAS-ELI­SA method and the virus was the most prevalent in the locality of Silbaš. During this investigation, Cucumber mosaic virus was present more often in mixed infections with ZYMV and/or WMV (12.66%), while the single infection was detected in lower percentage, 11.39%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and two of them were selected for further biological characterization. Concerning type and severity of symptoms on test plants, the investigated isolates exhibited different phenotypic features. Molecular detection was performed by amplification of a fragment of 871 bp in all tested isolates, using the primer pair CMVCPfwd/CPrev that amplify the entire coat protein gene and part of 5' and 3' UTR regions. The affiliation of Serbian CMV isolates from watermelon to subgroup IA, the group that includes most of the isolates selected for phylogenetic analysis, was determined by sequence analysis of the complete CP gene and reconstruction of phylogenetic tree., Proučavanjem pojave i rasprostranjenosti virusa mozaika krastavca u usevu lubenice u Srbiji tokom 2012. godine, prisustvo virusa utvrđeno je u pet od šest pregledanih lokaliteta. Prisustvo virusa ustanovljeno je u 24,05% serološki testiranih uzoraka (DAS-ELISA), a najzastupljeniji je bio na lokalitetu Silbaš. Tokom ovih istraživanja virus mozaika krastavca češće je bio prisutan u mešanim infekcijama sa ZYMV i/ili WMV (12,66%), dok je prisustvo pojedinačne infekcije utvrđeno u nešto nižem procentu, 11,39%. Mehaničkim inokulacijama N. glutinosa, dobijeno je pet izolata, od kojih su dva odabrana za dalju biološku karakterizaciju. Na osnovu tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem para prajmera CMVCPfwd/CPrev, koji omogućavaju umnožavanje gena za protein omotača i deo 5' i 3' UTR regiona. Analizom sekvenci kompletnog gena za protein omotača i rekonstrukcijom filogenetskog stabla, utvrđena je pripadnost izolata CMV iz lubenice iz Srbije podgrupi IA, koja obuhvata većinu izolata odabranih za filogenetsku analizu.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia, Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji",
pages = "25-14",
number = "1",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3242"
}

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Nikolić, D., Bulajić, A.,& Krstić, B.. (2013). Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 64(1), 14-25.
https://hdl.handle.net/21.15107/rcub_agrospace_3242

Milojević K, Stanković I, Vučurović A, Ristić D, Nikolić D, Bulajić A, Krstić B. Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia. in Zaštita bilja. 2013;64(1):14-25.
https://hdl.handle.net/21.15107/rcub_agrospace_3242 .

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Nikolić, Dušan, Bulajić, Aleksandra, Krstić, Branka, "Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia" in Zaštita bilja, 64, no. 1 (2013):14-25,
https://hdl.handle.net/21.15107/rcub_agrospace_3242 .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Dušan
AU  - Berenji, Janoš
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2608
AB  - A total of 39 samples of sorghum (Sorghum bicolor) with symptoms of stem and root rot were collected and analyzed during 2009-2011 in Bački Petrovac and Čantavir, Serbia. Monosporic cultures were isolated from stem tissue, their pathogenicity was confirmed by the development of symptoms on artificially inoculated sorghum plants, and they were identified on the basis of macroscopic and microscopic morphological features as Fusarium graminearum. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ef1/ ef2 and by amplification of protein coding TEF 1-alpha gen. Sequence of TEF gene from the selected isolate 535- 10 (JF747146) showed 98-99% nucleotide identity with sequences of 63 Gibberella zeae isolates deposited in NCBI GenBank. Amplification of the barcoding region of F. graminearum genome of sorghum isolate, contributes to the fast and accurate identification and characterization of Fusarium species in Serbia.
AB  - U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia
T1  - Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji
EP  - 352
IS  - 2
SP  - 347
VL  - 48
DO  - 10.5937/ratpov1102347R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ana and Stanković, Ivana and Nikolić, Dušan and Berenji, Janoš and Krstić, Branka and Bulajić, Aleksandra",
year = "2011",
abstract = "A total of 39 samples of sorghum (Sorghum bicolor) with symptoms of stem and root rot were collected and analyzed during 2009-2011 in Bački Petrovac and Čantavir, Serbia. Monosporic cultures were isolated from stem tissue, their pathogenicity was confirmed by the development of symptoms on artificially inoculated sorghum plants, and they were identified on the basis of macroscopic and microscopic morphological features as Fusarium graminearum. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ef1/ ef2 and by amplification of protein coding TEF 1-alpha gen. Sequence of TEF gene from the selected isolate 535- 10 (JF747146) showed 98-99% nucleotide identity with sequences of 63 Gibberella zeae isolates deposited in NCBI GenBank. Amplification of the barcoding region of F. graminearum genome of sorghum isolate, contributes to the fast and accurate identification and characterization of Fusarium species in Serbia., U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia, Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji",
pages = "352-347",
number = "2",
volume = "48",
doi = "10.5937/ratpov1102347R"
}

Ristić, D., Vučurović, A., Stanković, I., Nikolić, D., Berenji, J., Krstić, B.,& Bulajić, A.. (2011). Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(2), 347-352.
https://doi.org/10.5937/ratpov1102347R

Ristić D, Vučurović A, Stanković I, Nikolić D, Berenji J, Krstić B, Bulajić A. Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia. in Ratarstvo i povrtarstvo. 2011;48(2):347-352.
doi:10.5937/ratpov1102347R .

Ristić, Danijela, Vučurović, Ana, Stanković, Ivana, Nikolić, Dušan, Berenji, Janoš, Krstić, Branka, Bulajić, Aleksandra, "Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia" in Ratarstvo i povrtarstvo, 48, no. 2 (2011):347-352,
https://doi.org/10.5937/ratpov1102347R . .