Marjanović, Stefan

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orcid::0000-0002-5796-7023
  • Marjanović, Stefan (3)
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Author's Bibliography

Broccoli microgreens-apple juice as novel beverages: total phenolic, flavonoids and antioxidant activity

Belošević, Spasoje; Milinčić, Danijel; Salević-Jelić, Ana; Marković, Jovana; Lević, Steva; Pešić, Mirjana; Marjanović, Stefan; Đorđević, Verica; Nedović, Viktor

(University of Belgrade, Faculty of Technology and Metallurgy, 2023)

TY  - CONF
AU  - Belošević, Spasoje
AU  - Milinčić, Danijel
AU  - Salević-Jelić, Ana
AU  - Marković, Jovana
AU  - Lević, Steva
AU  - Pešić, Mirjana
AU  - Marjanović, Stefan
AU  - Đorđević, Verica
AU  - Nedović, Viktor
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6856
AB  - Recently, microgreens have been recognized as a potential food of the future, and
their application in the formulation of new products has been scarcely investigated. Only a
few studies promote the use of microgreens and sprouts in the formulation of novel products,
primarily beverages. The most often cultivated, analyzed and used microgreens are from the
Brassicaceae family, because they present a good source of bioactive compounds, primarily
glucosinolates, isothiocyanates and phenolic compounds. However, products from these
microgreens species have a typical flavor, with herbaceous, grassy and sulphurous notes,
often repulsive to consumers. Therefore, this study aims to examine total phenolic (TPC) and
flavonoids (TFC) content, as well as antioxidant properties (ABTS•+ and FRAP) of novel
sensorially acceptable broccoli microgreens-apple juice (BC-AJ). Previously produced coldpressed
broccoli microgreens and apple juices were mixed in the ratios 51% and 49%
respectively, and further analyzed by well-known spectrophotometric methods such as Folin-
Ciocalteu's (TPC) and aluminum chloride (TFC) methods, as well as methods based on
radical scavenging (ABTS•+) and ion reducing (FRAP) activities for evaluation of antioxidant
properties. Results for the TPC and TFC of BC-AJ were 109.78 ± 1.08 mg GAE/100 mL and
64.68 ± 2.25 mg QE/100 mL, respectively. Furthermore, novel BC-AJ had a good ability to
scavenge ABTS•+ radicals (162.90 ± 4.42 mg TE/100 mL) and a tendency to reduce [Fe3+-
(TPTZ)2]3+ complexes (258.50 ± 3.26 mg TE/100 mL), probably due to the most diverse of
phenolic compounds originated from broccoli and apple. Finally, broccoli microgreens-apple
juice has a high content of phenolic compounds and good antioxidant properties, so it can be
considered as a potentially functional beverage, but future research that includes additional in
vitro and in vivo studies is necessary.
PB  - University of Belgrade, Faculty of Technology and Metallurgy
C3  - Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists
T1  - Broccoli microgreens-apple juice as novel beverages: total phenolic, flavonoids and antioxidant activity
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6856
ER  - 
@conference{
author = "Belošević, Spasoje and Milinčić, Danijel and Salević-Jelić, Ana and Marković, Jovana and Lević, Steva and Pešić, Mirjana and Marjanović, Stefan and Đorđević, Verica and Nedović, Viktor",
year = "2023",
abstract = "Recently, microgreens have been recognized as a potential food of the future, and
their application in the formulation of new products has been scarcely investigated. Only a
few studies promote the use of microgreens and sprouts in the formulation of novel products,
primarily beverages. The most often cultivated, analyzed and used microgreens are from the
Brassicaceae family, because they present a good source of bioactive compounds, primarily
glucosinolates, isothiocyanates and phenolic compounds. However, products from these
microgreens species have a typical flavor, with herbaceous, grassy and sulphurous notes,
often repulsive to consumers. Therefore, this study aims to examine total phenolic (TPC) and
flavonoids (TFC) content, as well as antioxidant properties (ABTS•+ and FRAP) of novel
sensorially acceptable broccoli microgreens-apple juice (BC-AJ). Previously produced coldpressed
broccoli microgreens and apple juices were mixed in the ratios 51% and 49%
respectively, and further analyzed by well-known spectrophotometric methods such as Folin-
Ciocalteu's (TPC) and aluminum chloride (TFC) methods, as well as methods based on
radical scavenging (ABTS•+) and ion reducing (FRAP) activities for evaluation of antioxidant
properties. Results for the TPC and TFC of BC-AJ were 109.78 ± 1.08 mg GAE/100 mL and
64.68 ± 2.25 mg QE/100 mL, respectively. Furthermore, novel BC-AJ had a good ability to
scavenge ABTS•+ radicals (162.90 ± 4.42 mg TE/100 mL) and a tendency to reduce [Fe3+-
(TPTZ)2]3+ complexes (258.50 ± 3.26 mg TE/100 mL), probably due to the most diverse of
phenolic compounds originated from broccoli and apple. Finally, broccoli microgreens-apple
juice has a high content of phenolic compounds and good antioxidant properties, so it can be
considered as a potentially functional beverage, but future research that includes additional in
vitro and in vivo studies is necessary.",
publisher = "University of Belgrade, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists",
title = "Broccoli microgreens-apple juice as novel beverages: total phenolic, flavonoids and antioxidant activity",
pages = "68",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6856"
}
Belošević, S., Milinčić, D., Salević-Jelić, A., Marković, J., Lević, S., Pešić, M., Marjanović, S., Đorđević, V.,& Nedović, V.. (2023). Broccoli microgreens-apple juice as novel beverages: total phenolic, flavonoids and antioxidant activity. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists
University of Belgrade, Faculty of Technology and Metallurgy., 68.
https://hdl.handle.net/21.15107/rcub_agrospace_6856
Belošević S, Milinčić D, Salević-Jelić A, Marković J, Lević S, Pešić M, Marjanović S, Đorđević V, Nedović V. Broccoli microgreens-apple juice as novel beverages: total phenolic, flavonoids and antioxidant activity. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists. 2023;:68.
https://hdl.handle.net/21.15107/rcub_agrospace_6856 .
Belošević, Spasoje, Milinčić, Danijel, Salević-Jelić, Ana, Marković, Jovana, Lević, Steva, Pešić, Mirjana, Marjanović, Stefan, Đorđević, Verica, Nedović, Viktor, "Broccoli microgreens-apple juice as novel beverages: total phenolic, flavonoids and antioxidant activity" in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists (2023):68,
https://hdl.handle.net/21.15107/rcub_agrospace_6856 .

Characterization of amaranth (Amaranthus tricolor L.) microgreens juice encapsulated within inulin and maltodextrin

Belošević, Spasoje; Milinčić, Danijel; Salević-Jelić, Ana; Marković, Jovana; Lević, Steva; Pešić, Mirjana; Marjanović, Stefan; Đorđević, Verica; Nedović, Viktor

(University of Belgrade, Faculty of Technology and Metallurgy, 2023)

TY  - CONF
AU  - Belošević, Spasoje
AU  - Milinčić, Danijel
AU  - Salević-Jelić, Ana
AU  - Marković, Jovana
AU  - Lević, Steva
AU  - Pešić, Mirjana
AU  - Marjanović, Stefan
AU  - Đorđević, Verica
AU  - Nedović, Viktor
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6869
AB  - Amaranth microgreens represent a rich source of betalains and phenolic compounds,
which show a broad range of positive effects on human health. However, these
biocompounds are very sensitive and easily degraded, which often limits their application and
bioaccessibility. For the above reasons, natural extracts and juices are most often
encapsulated using different carriers, which protect and control the release of bioactive
compounds. The aim of this study was to encapsulate cold-pressed amaranth (Amaranthus
tricolor L.) microgreens juice using maltodextrin (AMD) and inulin (AIN) as carriers and to
investigate total phenolic content (TPC), total flavonoid content (TFC) and antioxidant
properties (ABTS•+ and FRAP) of obtained spray-dried powders. To the best of our
knowledge, this is the first report on the encapsulation of amaranth microgreens juice. Before
analysis, both powders were reconstituted in Milli-Q water (5% solutions) and analyzed using
well-known spectrophotometric methods. The results are expressed in mg equivalents (gallic
acid, quercetin, Trolox) per 100 g encapsulates. The obtained values for TPC and TFC for
AMD were 291.7 ± 3.0 mg GAE/100 g and 291.3 ± 2.5 mg QE/100 g, while values for AIN
were 356.9 ± 1.0 mg GAE/100 g and 289.6 ± 3.8 mg QE/100 g. The results of antioxidant
activity were as follows: 546.3 ± 12.6 mg TE/100 g (AMD) and 745.2 ± 3.1 mg TE/100 g
(AIN) for ABTS•+ and 713.3 ± 8.4 mg TE/100 g (AMD) and 905.1 ± 4.5 mg TE/100 g (AIN)
for FRAP. Finally, maltodextrin and inulin can be successfully used for the encapsulation of
bioactive compounds of amaranth microgreens. In addition, both powders show good
antioxidant properties and can be used in the food industry as potentially novel additives or
supplements.
PB  - University of Belgrade, Faculty of Technology and Metallurgy
C3  - Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists
T1  - Characterization of amaranth (Amaranthus tricolor L.) microgreens juice encapsulated within inulin and maltodextrin
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6869
ER  - 
@conference{
author = "Belošević, Spasoje and Milinčić, Danijel and Salević-Jelić, Ana and Marković, Jovana and Lević, Steva and Pešić, Mirjana and Marjanović, Stefan and Đorđević, Verica and Nedović, Viktor",
year = "2023",
abstract = "Amaranth microgreens represent a rich source of betalains and phenolic compounds,
which show a broad range of positive effects on human health. However, these
biocompounds are very sensitive and easily degraded, which often limits their application and
bioaccessibility. For the above reasons, natural extracts and juices are most often
encapsulated using different carriers, which protect and control the release of bioactive
compounds. The aim of this study was to encapsulate cold-pressed amaranth (Amaranthus
tricolor L.) microgreens juice using maltodextrin (AMD) and inulin (AIN) as carriers and to
investigate total phenolic content (TPC), total flavonoid content (TFC) and antioxidant
properties (ABTS•+ and FRAP) of obtained spray-dried powders. To the best of our
knowledge, this is the first report on the encapsulation of amaranth microgreens juice. Before
analysis, both powders were reconstituted in Milli-Q water (5% solutions) and analyzed using
well-known spectrophotometric methods. The results are expressed in mg equivalents (gallic
acid, quercetin, Trolox) per 100 g encapsulates. The obtained values for TPC and TFC for
AMD were 291.7 ± 3.0 mg GAE/100 g and 291.3 ± 2.5 mg QE/100 g, while values for AIN
were 356.9 ± 1.0 mg GAE/100 g and 289.6 ± 3.8 mg QE/100 g. The results of antioxidant
activity were as follows: 546.3 ± 12.6 mg TE/100 g (AMD) and 745.2 ± 3.1 mg TE/100 g
(AIN) for ABTS•+ and 713.3 ± 8.4 mg TE/100 g (AMD) and 905.1 ± 4.5 mg TE/100 g (AIN)
for FRAP. Finally, maltodextrin and inulin can be successfully used for the encapsulation of
bioactive compounds of amaranth microgreens. In addition, both powders show good
antioxidant properties and can be used in the food industry as potentially novel additives or
supplements.",
publisher = "University of Belgrade, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists",
title = "Characterization of amaranth (Amaranthus tricolor L.) microgreens juice encapsulated within inulin and maltodextrin",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6869"
}
Belošević, S., Milinčić, D., Salević-Jelić, A., Marković, J., Lević, S., Pešić, M., Marjanović, S., Đorđević, V.,& Nedović, V.. (2023). Characterization of amaranth (Amaranthus tricolor L.) microgreens juice encapsulated within inulin and maltodextrin. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists
University of Belgrade, Faculty of Technology and Metallurgy., 69.
https://hdl.handle.net/21.15107/rcub_agrospace_6869
Belošević S, Milinčić D, Salević-Jelić A, Marković J, Lević S, Pešić M, Marjanović S, Đorđević V, Nedović V. Characterization of amaranth (Amaranthus tricolor L.) microgreens juice encapsulated within inulin and maltodextrin. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists. 2023;:69.
https://hdl.handle.net/21.15107/rcub_agrospace_6869 .
Belošević, Spasoje, Milinčić, Danijel, Salević-Jelić, Ana, Marković, Jovana, Lević, Steva, Pešić, Mirjana, Marjanović, Stefan, Đorđević, Verica, Nedović, Viktor, "Characterization of amaranth (Amaranthus tricolor L.) microgreens juice encapsulated within inulin and maltodextrin" in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists (2023):69,
https://hdl.handle.net/21.15107/rcub_agrospace_6869 .

Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns

Vranković, Jelena; Božanić, Milenka; Živić, Miroslav; Marković, Zoran; Marjanović, Stefan; Golubović, Vukosav; Živić, Ivana

(2022)

TY  - JOUR
AU  - Vranković, Jelena
AU  - Božanić, Milenka
AU  - Živić, Miroslav
AU  - Marković, Zoran
AU  - Marjanović, Stefan
AU  - Golubović, Vukosav
AU  - Živić, Ivana
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6210
AB  - A 102-day feeding trial was conducted to evaluate the effects of four different fish feeding patterns on carp pond water quality and antioxidant biomarkers [superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione S-transferase (GST) and Thiol groups (SH)], protein content and biomass of the midge Chironomus plumosus. Farmed fish were fed two commercial diets: Soprofish 25/7 Standard (containing 25% protein and 7 % fat) and Soprofish 32/7 Profi Effect (containing 32 % protein and 7 % fat). These feeds were combined during a feeding trial in four different feeding patterns, designated as A, B, C and D. In feeding pattern A, the fish received Soprofish 25/7 Standard throughout the experiment and in feeding pattern D, Soprofish 32/7 Profi Effect. During feeding patterns B and C, a mixed feeding pattern was used, alternating between lower and higher protein diets. The study revealed no significant effects (P > 0.05) of the feeding regimens on water quality, so their effects on C. plumosus larvae can be evaluated independently. The activities of the chironomid enzymes CAT and GR showed a clear statistically significant dependence on the feeding pattern (P < 0.05), which increased with increasing protein content. On the other hand, the activity of SOD as well as larval biomass were noticeably affected by water quality, the former increasing with its deterioration and the latter with its improvement. The activities of GPx and GST were correlated with each other, but no statistically significant relationship was found with any of the measured abiotic factors (P > 0.05). Overall, our results suggest that long-term supplementation with a highly concentrated protein diet may have preventive effects against oxidative stress and support the use of C. plumosus as a model for assessing the effects of organic pollution on pond zoobenthic fauna. © 2022
T2  - Aquaculture Reports
T2  - Aquaculture Reports
T1  - Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns
VL  - 27
DO  - 10.1016/j.aqrep.2022.101387
ER  - 
@article{
author = "Vranković, Jelena and Božanić, Milenka and Živić, Miroslav and Marković, Zoran and Marjanović, Stefan and Golubović, Vukosav and Živić, Ivana",
year = "2022",
abstract = "A 102-day feeding trial was conducted to evaluate the effects of four different fish feeding patterns on carp pond water quality and antioxidant biomarkers [superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR), Glutathione S-transferase (GST) and Thiol groups (SH)], protein content and biomass of the midge Chironomus plumosus. Farmed fish were fed two commercial diets: Soprofish 25/7 Standard (containing 25% protein and 7 % fat) and Soprofish 32/7 Profi Effect (containing 32 % protein and 7 % fat). These feeds were combined during a feeding trial in four different feeding patterns, designated as A, B, C and D. In feeding pattern A, the fish received Soprofish 25/7 Standard throughout the experiment and in feeding pattern D, Soprofish 32/7 Profi Effect. During feeding patterns B and C, a mixed feeding pattern was used, alternating between lower and higher protein diets. The study revealed no significant effects (P > 0.05) of the feeding regimens on water quality, so their effects on C. plumosus larvae can be evaluated independently. The activities of the chironomid enzymes CAT and GR showed a clear statistically significant dependence on the feeding pattern (P < 0.05), which increased with increasing protein content. On the other hand, the activity of SOD as well as larval biomass were noticeably affected by water quality, the former increasing with its deterioration and the latter with its improvement. The activities of GPx and GST were correlated with each other, but no statistically significant relationship was found with any of the measured abiotic factors (P > 0.05). Overall, our results suggest that long-term supplementation with a highly concentrated protein diet may have preventive effects against oxidative stress and support the use of C. plumosus as a model for assessing the effects of organic pollution on pond zoobenthic fauna. © 2022",
journal = "Aquaculture Reports, Aquaculture Reports",
title = "Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns",
volume = "27",
doi = "10.1016/j.aqrep.2022.101387"
}
Vranković, J., Božanić, M., Živić, M., Marković, Z., Marjanović, S., Golubović, V.,& Živić, I.. (2022). Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns. in Aquaculture Reports, 27.
https://doi.org/10.1016/j.aqrep.2022.101387
Vranković J, Božanić M, Živić M, Marković Z, Marjanović S, Golubović V, Živić I. Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns. in Aquaculture Reports. 2022;27.
doi:10.1016/j.aqrep.2022.101387 .
Vranković, Jelena, Božanić, Milenka, Živić, Miroslav, Marković, Zoran, Marjanović, Stefan, Golubović, Vukosav, Živić, Ivana, "Antioxidant biomarker profile of chironomid larvae from carp ponds: Evaluation of the effects of different fish feeding patterns" in Aquaculture Reports, 27 (2022),
https://doi.org/10.1016/j.aqrep.2022.101387 . .