TY  - CONF
AU  - Sknepnek, Aleksandra
AU  - Miletić, Dunja
AU  - Mrkonjić, Živan
AU  - Zeković, Zoran
AU  - Nedović, Viktor
AU  - Pavlić, Branimir
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6832
AB  - Antimicrobial resistance to antibiotics is growing, affecting increased burden of the health
system and higher incidence of human fatal outcomes. Therefore, the research of novel
antimicrobial substances is of crucial importance. Wild thyme (Thymus serpyllum L.) extracts
were obtained using different conditions of green and environmentally-friendly supercritical
fluid extraction. Sample SFE-TSŽ2 was prepared at 350 bar and 50 °C, while SFE-TSŽ7 was
extracted at 100 bar and 40 °C. Broth microdilution method was applied to determine how
different extraction conditions reflect on sample antimicrobial activity against pathogenic
microorganisms. Two Gram positive strains, Staphylococcus aureus ATCC 25923 and
methicillin-resistant Staphylococcus aureus (MRSA), stood out as the most sensitive to both
samples (minimal inhibitory concentration, MIC value below 0.02 mg/mL). Bactericidal effect
of the sample SFE-TSŽ2 was found for all Gram-positive strains, with minimal bactericidal
concentrations (MBC) lower than for SFE-TSŽ7, except for Staphylococcus aureus (MRSA)
when the same effect of both extracts was found. Significant difference between samples was
observed in inhibitory activity against Gram-negative bacterial strains, revealing higher
efficiency of SFE-TSŽ2 compared to SFE-TSŽ7. The most sensitive to both samples were
Proteus hauseri ATCC 13315 and Yersinia enterocolitica ATCC 27729 with the same MIC
values (0.83 mg/mL for SFE-TSŽ2 and 2.5 mg/mL for SFE-TSŽ7). SFE-TSŽ2 also expressed
inhibitory and fungicidal effects against Candida albicans ATCC 1231 yeast (MIC 2.5 mg/mL;
minimal fungicidal concentration, MFC, 10 mg/mL), while SFE-TSŽ7 showed no activity.
Obtained results showed that applied extraction conditions affected antimicrobial activities,
most probably due to the differences in chemical compositions.
PB  - University of Novi Sad, Faculty of Technology Novi Sad,Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
C3  - 2nd International Conference on Advanced Production and Processing (ICAPP 2022)
T1  - ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS
SP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6832
ER  - 

@conference{
author = "Sknepnek, Aleksandra and Miletić, Dunja and Mrkonjić, Živan and Zeković, Zoran and Nedović, Viktor and Pavlić, Branimir",
year = "2022",
abstract = "Antimicrobial resistance to antibiotics is growing, affecting increased burden of the health
system and higher incidence of human fatal outcomes. Therefore, the research of novel
antimicrobial substances is of crucial importance. Wild thyme (Thymus serpyllum L.) extracts
were obtained using different conditions of green and environmentally-friendly supercritical
fluid extraction. Sample SFE-TSŽ2 was prepared at 350 bar and 50 °C, while SFE-TSŽ7 was
extracted at 100 bar and 40 °C. Broth microdilution method was applied to determine how
different extraction conditions reflect on sample antimicrobial activity against pathogenic
microorganisms. Two Gram positive strains, Staphylococcus aureus ATCC 25923 and
methicillin-resistant Staphylococcus aureus (MRSA), stood out as the most sensitive to both
samples (minimal inhibitory concentration, MIC value below 0.02 mg/mL). Bactericidal effect
of the sample SFE-TSŽ2 was found for all Gram-positive strains, with minimal bactericidal
concentrations (MBC) lower than for SFE-TSŽ7, except for Staphylococcus aureus (MRSA)
when the same effect of both extracts was found. Significant difference between samples was
observed in inhibitory activity against Gram-negative bacterial strains, revealing higher
efficiency of SFE-TSŽ2 compared to SFE-TSŽ7. The most sensitive to both samples were
Proteus hauseri ATCC 13315 and Yersinia enterocolitica ATCC 27729 with the same MIC
values (0.83 mg/mL for SFE-TSŽ2 and 2.5 mg/mL for SFE-TSŽ7). SFE-TSŽ2 also expressed
inhibitory and fungicidal effects against Candida albicans ATCC 1231 yeast (MIC 2.5 mg/mL;
minimal fungicidal concentration, MFC, 10 mg/mL), while SFE-TSŽ7 showed no activity.
Obtained results showed that applied extraction conditions affected antimicrobial activities,
most probably due to the differences in chemical compositions.",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad,Bulevar cara Lazara 1, 21000 Novi Sad, Serbia",
journal = "2nd International Conference on Advanced Production and Processing (ICAPP 2022)",
title = "ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS",
pages = "170",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6832"
}

Sknepnek, A., Miletić, D., Mrkonjić, Ž., Zeković, Z., Nedović, V.,& Pavlić, B.. (2022). ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS. in 2nd International Conference on Advanced Production and Processing (ICAPP 2022)
University of Novi Sad, Faculty of Technology Novi Sad,Bulevar cara Lazara 1, 21000 Novi Sad, Serbia., 170.
https://hdl.handle.net/21.15107/rcub_agrospace_6832

Sknepnek A, Miletić D, Mrkonjić Ž, Zeković Z, Nedović V, Pavlić B. ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS. in 2nd International Conference on Advanced Production and Processing (ICAPP 2022). 2022;:170.
https://hdl.handle.net/21.15107/rcub_agrospace_6832 .

Sknepnek, Aleksandra, Miletić, Dunja, Mrkonjić, Živan, Zeković, Zoran, Nedović, Viktor, Pavlić, Branimir, "ANTIMICROBIAL ACTIVITY OF SUPERCRITICAL EXTRACTS FROM WILD THYME (THYMUS SERPYLLUM L.) BY-PRODUCTS" in 2nd International Conference on Advanced Production and Processing (ICAPP 2022) (2022):170,
https://hdl.handle.net/21.15107/rcub_agrospace_6832 .