TY  - CONF
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Salević, Ana
AU  - Radovanović, Željko
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6868
AB  - Leaves available as by-products from some crops could be used as a major protein
source for food applications. Here, pumpkin leaves from field crop side streams were used for
the preparation of a protein-rich extract. Namely, pumpkin leaves were mechanically
processed with additional steps of acidic treatment and lyophilization to produce the extract.
The extract (1 mass%) was mixed with a spinnable biopolymer-pullulan (5 mass%) with the
addition of vitamin B12 (vitB12) as a model vitamin. This homogeneous mixture was
electrospuned through a stainless-steel needle (18G) at a steady flow rate of 25.2 mL/h using
a syringe pump and an electric field (17 kV) between the positively charged needle and
grounded metallic collector plate. The starting solutions (plain pullulan-PUL, pullulan with
extract-PUL+E, and pullulan with extract and vitB12-PUL+E+B12) as well as the resulting
structures were analyzed regarding physicochemical and morphological properties.
The addition of the extract increases the surface tension of the PUL solution, but
vitB12 decreases it. On the other hand, the viscosity measurements showed an increase in the
value with the addition of extract and vitB12. PUL solution had a conductivity of 0.163
mS/cm, and the value increased upon adding the extract and vitB12 (1.420 mS/cm and 0.978
mS/cm, respectively). According to SEM images, electrospinning resulted in the formation of
beaded fibers. The lower the viscosity of the solution, the greater the proportion of particles
observed, which is in accordance with literature data. The mean value of the particle size
decreases from 388.89 nm (PUL) to 176.68 nm (PUL+E). However, the addition of vitB12
leads to an increase in the mean particle size to 255.94 nm. The addition of extract didn't have
a strong effect on average fiber diameter, while a slight increase was observed in
PUL+E+B12. FTIR analysis suggests intermolecular interactions between the constituents of
the pullulan-extract-vitamin B12 fibers. As a conclusion, the combination of pullulan and
protein-rich extract has the properties of a stable carrier for the encapsulation of vitamin B12.
PB  - University of Belgrade, Faculty of Technology and Metallurgy
C3  - Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists
T1  - Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_6868
ER  - 

@conference{
author = "Balanč, Bojana and Đorđević, Verica and Salević, Ana and Radovanović, Željko and Nedović, Viktor and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2023",
abstract = "Leaves available as by-products from some crops could be used as a major protein
source for food applications. Here, pumpkin leaves from field crop side streams were used for
the preparation of a protein-rich extract. Namely, pumpkin leaves were mechanically
processed with additional steps of acidic treatment and lyophilization to produce the extract.
The extract (1 mass%) was mixed with a spinnable biopolymer-pullulan (5 mass%) with the
addition of vitamin B12 (vitB12) as a model vitamin. This homogeneous mixture was
electrospuned through a stainless-steel needle (18G) at a steady flow rate of 25.2 mL/h using
a syringe pump and an electric field (17 kV) between the positively charged needle and
grounded metallic collector plate. The starting solutions (plain pullulan-PUL, pullulan with
extract-PUL+E, and pullulan with extract and vitB12-PUL+E+B12) as well as the resulting
structures were analyzed regarding physicochemical and morphological properties.
The addition of the extract increases the surface tension of the PUL solution, but
vitB12 decreases it. On the other hand, the viscosity measurements showed an increase in the
value with the addition of extract and vitB12. PUL solution had a conductivity of 0.163
mS/cm, and the value increased upon adding the extract and vitB12 (1.420 mS/cm and 0.978
mS/cm, respectively). According to SEM images, electrospinning resulted in the formation of
beaded fibers. The lower the viscosity of the solution, the greater the proportion of particles
observed, which is in accordance with literature data. The mean value of the particle size
decreases from 388.89 nm (PUL) to 176.68 nm (PUL+E). However, the addition of vitB12
leads to an increase in the mean particle size to 255.94 nm. The addition of extract didn't have
a strong effect on average fiber diameter, while a slight increase was observed in
PUL+E+B12. FTIR analysis suggests intermolecular interactions between the constituents of
the pullulan-extract-vitamin B12 fibers. As a conclusion, the combination of pullulan and
protein-rich extract has the properties of a stable carrier for the encapsulation of vitamin B12.",
publisher = "University of Belgrade, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists",
title = "Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6868"
}

Balanč, B., Đorđević, V., Salević, A., Radovanović, Ž., Nedović, V., Bugarski, B.,& Knežević-Jugović, Z.. (2023). Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists
University of Belgrade, Faculty of Technology and Metallurgy., 59.
https://hdl.handle.net/21.15107/rcub_agrospace_6868

Balanč B, Đorđević V, Salević A, Radovanović Ž, Nedović V, Bugarski B, Knežević-Jugović Z. Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12. in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists. 2023;:59.
https://hdl.handle.net/21.15107/rcub_agrospace_6868 .

Balanč, Bojana, Đorđević, Verica, Salević, Ana, Radovanović, Željko, Nedović, Viktor, Bugarski, Branko, Knežević-Jugović, Zorica, "Nanostructures based on pullulan and pumpkin green leaf proteins as carriers for vitamin B12" in Book of Abstracts of the International Conference on Biochemical Engineering and Biotechnology for Young Scientists (2023):59,
https://hdl.handle.net/21.15107/rcub_agrospace_6868 .

TY  - JOUR
AU  - Popović, Ana L.
AU  - Velicković, Zlate
AU  - Radovanović, Željko
AU  - Đolić, Maja
AU  - Pavlović, Vladimir
AU  - Marinković, Aleksandar D.
AU  - Gržetić, Jelena D.
PY  - 2022
UR  - https://www.sciencedirect.com/science/article/pii/S221334372200882X
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6113
AB  - Highly effective lignin-based microspheres were prepared by inverse copolymerization of lignin (kraft) from suspension, with poly(ethylene imine) and amino-functionalized magnetite nanoparticles (A-LMS_Fe3O4) or manganese(IV)oxide nanoparticles (A-LMS_MnO2). The XRD, FTIR, SEM, BET, TEM techniques, including the porosity determination, were performed to analyze morphological and structural properties of synthesized microspheres. The effect of pH, the adsorbent dosage, temperature and contact duration on a batch-mode adsorption efficiency of arsenate and chromate (oxy)anions removal was tested. Spontaneous adsorption was found to be feasible, reaching the adsorption capacities of Cr(VI) (62.9 mg g−1), As(V) (47.8 mg g−1) by A-LMS_Fe3O4, and of Cr(VI) (73.9 mg g−1), As(V) (62.5 mg g−1) using A-LMS_MnO2 adsorbent. Fast removal rates were confirmed via kinetic study, using pseudo-second order, the Weber-Morris and the single resistance mass-transfer model, with a limiting step of the diffusion transport through pores. The correlation of fixed-bed-column results with Bohart–Adams, Thomas, Yoon–Nelson and Dose Response models displayed that breakthrough behavior was influenced by flow rate and the inlet concentration. Significant potential of produced bio-adsorbent is further confirmed by the pore surface diffusion modeling, desorption study and adsorption from multi-component system using artificial water.
T2  - Journal of Environmental Chemical Engineering
T2  - Journal of Environmental Chemical EngineeringJournal of Environmental Chemical Engineering
T1  - Hybrid amino-terminated lignin microspheres loaded with magnetite and manganese oxide nanoparticles: An effective hazardous oxyanions adsorbent
IS  - 3
SP  - 108009
VL  - 10
DO  - 10.1016/j.jece.2022.108009
ER  - 

@article{
author = "Popović, Ana L. and Velicković, Zlate and Radovanović, Željko and Đolić, Maja and Pavlović, Vladimir and Marinković, Aleksandar D. and Gržetić, Jelena D.",
year = "2022",
abstract = "Highly effective lignin-based microspheres were prepared by inverse copolymerization of lignin (kraft) from suspension, with poly(ethylene imine) and amino-functionalized magnetite nanoparticles (A-LMS_Fe3O4) or manganese(IV)oxide nanoparticles (A-LMS_MnO2). The XRD, FTIR, SEM, BET, TEM techniques, including the porosity determination, were performed to analyze morphological and structural properties of synthesized microspheres. The effect of pH, the adsorbent dosage, temperature and contact duration on a batch-mode adsorption efficiency of arsenate and chromate (oxy)anions removal was tested. Spontaneous adsorption was found to be feasible, reaching the adsorption capacities of Cr(VI) (62.9 mg g−1), As(V) (47.8 mg g−1) by A-LMS_Fe3O4, and of Cr(VI) (73.9 mg g−1), As(V) (62.5 mg g−1) using A-LMS_MnO2 adsorbent. Fast removal rates were confirmed via kinetic study, using pseudo-second order, the Weber-Morris and the single resistance mass-transfer model, with a limiting step of the diffusion transport through pores. The correlation of fixed-bed-column results with Bohart–Adams, Thomas, Yoon–Nelson and Dose Response models displayed that breakthrough behavior was influenced by flow rate and the inlet concentration. Significant potential of produced bio-adsorbent is further confirmed by the pore surface diffusion modeling, desorption study and adsorption from multi-component system using artificial water.",
journal = "Journal of Environmental Chemical Engineering, Journal of Environmental Chemical EngineeringJournal of Environmental Chemical Engineering",
title = "Hybrid amino-terminated lignin microspheres loaded with magnetite and manganese oxide nanoparticles: An effective hazardous oxyanions adsorbent",
number = "3",
pages = "108009",
volume = "10",
doi = "10.1016/j.jece.2022.108009"
}

Popović, A. L., Velicković, Z., Radovanović, Ž., Đolić, M., Pavlović, V., Marinković, A. D.,& Gržetić, J. D.. (2022). Hybrid amino-terminated lignin microspheres loaded with magnetite and manganese oxide nanoparticles: An effective hazardous oxyanions adsorbent. in Journal of Environmental Chemical Engineering, 10(3), 108009.
https://doi.org/10.1016/j.jece.2022.108009

Popović AL, Velicković Z, Radovanović Ž, Đolić M, Pavlović V, Marinković AD, Gržetić JD. Hybrid amino-terminated lignin microspheres loaded with magnetite and manganese oxide nanoparticles: An effective hazardous oxyanions adsorbent. in Journal of Environmental Chemical Engineering. 2022;10(3):108009.
doi:10.1016/j.jece.2022.108009 .

Popović, Ana L., Velicković, Zlate, Radovanović, Željko, Đolić, Maja, Pavlović, Vladimir, Marinković, Aleksandar D., Gržetić, Jelena D., "Hybrid amino-terminated lignin microspheres loaded with magnetite and manganese oxide nanoparticles: An effective hazardous oxyanions adsorbent" in Journal of Environmental Chemical Engineering, 10, no. 3 (2022):108009,
https://doi.org/10.1016/j.jece.2022.108009 . .