TY  - JOUR
AU  - Decleer, Marlies
AU  - Jovanović, Jelena
AU  - Vakula, Anita
AU  - Udovički, Božidar
AU  - Agoua, Rock-Seth E.K.
AU  - Madder, Annemieke
AU  - De Saeger, Sarah
AU  - Rajković, Andreja
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4759
AB  - The emetic syndrome of Bacillus cereus is a food intoxication caused by cereulide (CER) and manifested by emesis, nausea and in most severe cases with liver failure. While acute effects have been studied in the aftermath of food intoxication, an exposure to low doses of cereulide might cause unnoticed damages to the intestines and liver. The toxicity which relies on the mitochondrial dysfunction was assessed on Caco-2 and HepG2 cells after exposure of one, three and ten days to a range of low doses of cereulide. Oxygen consumption rate analyses were used to study the impact of low doses of CER on the bioenergetics functions of undifferentiated Caco-2 and HepG2 cells using Seahorse XF extracellular flux analyzer. Both Caco-2 and HepG2 cells experienced measurable mitochondrial impairment after prolonged exposure of 10 days to 0.25 nM of cereulide. Observed mitochondrial dysfunction was greatly reflected in reduction of maximal cell respiration. At 0.50 nM CER, mitochondrial respiration was almost completely shut down, especially in HepG2 cells. These results corresponded with a severe reduction in the amount of cells and an altered morphology, observed by microscopic examination of the cells. Accurate and robust quantification of basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration allowed better understanding of the effects of cereulide in underlying respiratory malfunctions in low-dose exposure.
PB  - MDPI, BASEL
T2  - Toxins
T1  - Oxygen Consumption Rate Analysis of Mitochondrial Dysfunction Caused by Bacillus cereus Cereulide in Caco-2 and HepG2 Cells
IS  - 7
VL  - 10
DO  - 10.3390/toxins10070266
ER  - 

@article{
author = "Decleer, Marlies and Jovanović, Jelena and Vakula, Anita and Udovički, Božidar and Agoua, Rock-Seth E.K. and Madder, Annemieke and De Saeger, Sarah and Rajković, Andreja",
year = "2018",
abstract = "The emetic syndrome of Bacillus cereus is a food intoxication caused by cereulide (CER) and manifested by emesis, nausea and in most severe cases with liver failure. While acute effects have been studied in the aftermath of food intoxication, an exposure to low doses of cereulide might cause unnoticed damages to the intestines and liver. The toxicity which relies on the mitochondrial dysfunction was assessed on Caco-2 and HepG2 cells after exposure of one, three and ten days to a range of low doses of cereulide. Oxygen consumption rate analyses were used to study the impact of low doses of CER on the bioenergetics functions of undifferentiated Caco-2 and HepG2 cells using Seahorse XF extracellular flux analyzer. Both Caco-2 and HepG2 cells experienced measurable mitochondrial impairment after prolonged exposure of 10 days to 0.25 nM of cereulide. Observed mitochondrial dysfunction was greatly reflected in reduction of maximal cell respiration. At 0.50 nM CER, mitochondrial respiration was almost completely shut down, especially in HepG2 cells. These results corresponded with a severe reduction in the amount of cells and an altered morphology, observed by microscopic examination of the cells. Accurate and robust quantification of basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration allowed better understanding of the effects of cereulide in underlying respiratory malfunctions in low-dose exposure.",
publisher = "MDPI, BASEL",
journal = "Toxins",
title = "Oxygen Consumption Rate Analysis of Mitochondrial Dysfunction Caused by Bacillus cereus Cereulide in Caco-2 and HepG2 Cells",
number = "7",
volume = "10",
doi = "10.3390/toxins10070266"
}

Decleer, M., Jovanović, J., Vakula, A., Udovički, B., Agoua, R. E.K., Madder, A., De Saeger, S.,& Rajković, A.. (2018). Oxygen Consumption Rate Analysis of Mitochondrial Dysfunction Caused by Bacillus cereus Cereulide in Caco-2 and HepG2 Cells. in Toxins
MDPI, BASEL., 10(7).
https://doi.org/10.3390/toxins10070266

Decleer M, Jovanović J, Vakula A, Udovički B, Agoua RE, Madder A, De Saeger S, Rajković A. Oxygen Consumption Rate Analysis of Mitochondrial Dysfunction Caused by Bacillus cereus Cereulide in Caco-2 and HepG2 Cells. in Toxins. 2018;10(7).
doi:10.3390/toxins10070266 .

Decleer, Marlies, Jovanović, Jelena, Vakula, Anita, Udovički, Božidar, Agoua, Rock-Seth E.K., Madder, Annemieke, De Saeger, Sarah, Rajković, Andreja, "Oxygen Consumption Rate Analysis of Mitochondrial Dysfunction Caused by Bacillus cereus Cereulide in Caco-2 and HepG2 Cells" in Toxins, 10, no. 7 (2018),
https://doi.org/10.3390/toxins10070266 . .