@conference{
author = "Milinčić, Danijel and Petrović, Jovana and Glamočlija, Jasmina and Gašić, Uroš and Doroški, Ana and Kostić, Aleksandar and Stanojević, Slađana and Pešić, Mirjana B.",
year = "2023",
abstract = "The application of mushrooms for medicinal purposes has a long history, primarily due to its therapeutic properties. Today,
mushrooms are often used as functional food or natural sources in the development of various nutraceuticals. Using advanced
instrumental techniques, it was shown that mushrooms are a good source of highly valuable polysaccharides (i.e.,
glucans), sterols (i.e., ergosterol), different antioxidants, proteins and peptides. However, due to the great diversity of fungi,
additional research in this area should be performed. The aim of this study is to analyze biocompounds from polysaccharides
and aqueous extracts of two different mushrooms (A. bisporus and A. aegerita). Mushroom extracts were prepared
according to procedure previously desribed by Popović Minić (2023)[1]. Lyophilised mushroom powder was extracted with
80% methanol containing 0.1% HCl, after which the suspension was filtered through 0.45μm filters and used for further
chromatographic analysis by UHPLC-QToF-MS. Chemical characterization of mushroom biomolecules was performed
using exact mass (m/z) and MS2 fragment ions of each detected compound and their retention times. The identified compounds
represented four structurally distinct groups: 1) organic acids and their derivatives (7 compounds); 2) phenolic
acids and their derivatives (11 compounds); 3) esters (28 compounds); and 4) other organic compounds (Gibberellin A1 ).
Based on the obtained results, the differences between the tested samples can be clearly observed. In A.bisposrus and
A.aegerita polysaccharide extracts only few organic acids and esters were detected, while phenolics and majority of esters
were not recorded. On the other hand, the presence of organic acids, phenolic acids, esters and their derivatives was confirmed
in both aqueous extracts. The highest number of detected compounds (as many as 41 compounds) was detected in
the aqueous extract of A. aegerita. Among organic acids, fumaric, malic and citric acids were detected in all the mushroom
extracts, whereas p-hydroxybenzoic acid, m-hydroxy-hydrocinnamic acid, sinapic acid, 2-(pentanoyloxy)benzoate, and
3-(11-hydroxyundecoxy) benzoate were detected among phenolic acids and their derivatives in aqueous extracts of both
mushrooms. Regarding detected esters, following compounds were identified in the tested samples: 8-carboxyoctanoate,
3-(octyloxy)-3-oxopropanoate, 9,12,13-trihydroxyoctadecenoate, 13-hydroxy-9,11-octadecadienoate. The estimated profiles
of biocompounds present in mushroom extracts can contribute to the further understanding of their antioxidant and
biological properties.",
publisher = "Srpsko hemijsko društvo, Karnegijeva 4, Beograd 11000",
journal = "XXII Congress EuroFoodChem",
title = "Biocompounds from mushroom aqueous and polysaccharide extracts",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_6522"
}
