TY  - JOUR
AU  - Volić, Mina
AU  - Pećinar, Ilinka
AU  - Micić, Darko
AU  - Đorđević, Verica
AU  - Pešić, Radojica
AU  - Nedović, Viktor
AU  - Obradović, Nataša
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6049
AB  - Innovative coating powders, based on whey protein concentrate (10–15 wt%) as native (WPC) or denatured protein (D-WPC), solely or in combination with alginate (0.75 wt%, AL), containing thyme essential oil, were produced using the freeze-drying technique. The impact of individual components (protein, alginate and oil) as well as the effect of heat-induced protein denaturation, was resolved regarding physicochemical, thermal and morphological properties of powders. High product yield (∼100%), particle size (223–257 nm), low moisture content (0.10–0.13%) and zeta potential (−19 to −25.6 mV) were determined for all samples. Strong antimicrobial activity of thyme oil nanocarriers against foodborne pathogens was demonstrated. Thermogravimetric analysis (TGA) indicated enhanced thermal stability of encapsulated oil. The most specific bands of structural compounds were identified in Raman spectra of the tested formulations, but principal component analysis (PCA) on recorded spectra was necessary to show the differences between carriers of different wall materials.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying
SP  - 132749
VL  - 386
DO  - 10.1016/j.foodchem.2022.132749
ER  - 

@article{
author = "Volić, Mina and Pećinar, Ilinka and Micić, Darko and Đorđević, Verica and Pešić, Radojica and Nedović, Viktor and Obradović, Nataša",
year = "2022",
abstract = "Innovative coating powders, based on whey protein concentrate (10–15 wt%) as native (WPC) or denatured protein (D-WPC), solely or in combination with alginate (0.75 wt%, AL), containing thyme essential oil, were produced using the freeze-drying technique. The impact of individual components (protein, alginate and oil) as well as the effect of heat-induced protein denaturation, was resolved regarding physicochemical, thermal and morphological properties of powders. High product yield (∼100%), particle size (223–257 nm), low moisture content (0.10–0.13%) and zeta potential (−19 to −25.6 mV) were determined for all samples. Strong antimicrobial activity of thyme oil nanocarriers against foodborne pathogens was demonstrated. Thermogravimetric analysis (TGA) indicated enhanced thermal stability of encapsulated oil. The most specific bands of structural compounds were identified in Raman spectra of the tested formulations, but principal component analysis (PCA) on recorded spectra was necessary to show the differences between carriers of different wall materials.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying",
pages = "132749",
volume = "386",
doi = "10.1016/j.foodchem.2022.132749"
}

Volić, M., Pećinar, I., Micić, D., Đorđević, V., Pešić, R., Nedović, V.,& Obradović, N.. (2022). Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying. in Food Chemistry
Elsevier Ltd., 386, 132749.
https://doi.org/10.1016/j.foodchem.2022.132749

Volić M, Pećinar I, Micić D, Đorđević V, Pešić R, Nedović V, Obradović N. Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying. in Food Chemistry. 2022;386:132749.
doi:10.1016/j.foodchem.2022.132749 .

Volić, Mina, Pećinar, Ilinka, Micić, Darko, Đorđević, Verica, Pešić, Radojica, Nedović, Viktor, Obradović, Nataša, "Design and characterization of whey protein nanocarriers for thyme essential oil encapsulation obtained by freeze-drying" in Food Chemistry, 386 (2022):132749,
https://doi.org/10.1016/j.foodchem.2022.132749 . .

TY  - JOUR
AU  - Nedović, Viktor
AU  - Leskošek-Čukalović, Ida
AU  - Trifunović, Olivera
AU  - Pešić, Radojica
AU  - Bugarski, Branko
PY  - 2000
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/172
AB  - In beer brewing with immobilized yeast cells it is very important to minimize the mass transfer resistance, problem associated with large diameter beads, because the mass transfer limitations may force the cells to alter their metabolic states and thus, impact the efficiency of fermentation process and quality of final beer. Internal mass transfer can be optimized by adjusting the immobilization matrix size, texture and porosity. The classical dripping method, that is commonly used to produce gel beads, has a limitation in the large diameter of the produced beads, which is typically 2 to 3 mm. In recent time other techniques have been developed to produce smaller particles of equal size. The major objective of this study was to optimize the bead size by using the new technique, electrostatic droplet generation. We have obtained promising results since in this way it was possible to produce very small uniform microbeads (in the diameter range of several hundreds of micrometers to 2 mm) under stable conditions. The size of beads was strongly influenced by applied potential, needle size and electrode distance.
AB  - Pri primeni imobilisanih ćelija kvasca u fermentaciji piva važno je, kako sa aspekta produktivnosti sistema, tako i sa aspekta hemijskog sastava piva, da se otpori prenosu mase supstrata i proizvoda metabolizma ćelija unutar matrice nosača svedu na najmanju moguću meru. Ovo se može postići podešavanjem veličine, teksture i poroznosti matrice nosača. Obzirom da klasična tehnika imobilizacije kvasca u matrici Ca-alginata daje čestice većih dimenzija (prečnika od 2 do 3 mm), danas je razvijeno vise različitih tehnika koje pružaju mogućnost dobijanja čestica malih dimenzija. Jedna od novih tehnika, čije su mogućnosti primene u cilju optimizacije veličine alginatnih čestica ispitivane u sklopu ovog istraživanja, je postupak elektrostatičke ekstruzije. Dobijeni rezultati su pokazali daje primenom ove tehnike moguće dobiti kontrolisanu i stabilnu proizvodnju mikro čestica uniformne veličine, čiji se prečnik kretao u rasponu od nekoliko stotina mikrometara do 2 mm, u zavisnosti od primenjene razlike potencijala, prečnika igle i rastojanja između elektroda.
PB  - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad
T2  - Acta periodica technologica
T1  - Immobilization of yeast cells in small alginate beads using electrostatic droplet generation
T1  - Primena postupka elektrostatičke ekstruzije pri dobijanju alginatnih čestica malih dimenzija sa imobilisanim ćelijama pivskog kvasca
EP  - 597
IS  - 31
SP  - 591
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_172
ER  - 

@article{
author = "Nedović, Viktor and Leskošek-Čukalović, Ida and Trifunović, Olivera and Pešić, Radojica and Bugarski, Branko",
year = "2000",
abstract = "In beer brewing with immobilized yeast cells it is very important to minimize the mass transfer resistance, problem associated with large diameter beads, because the mass transfer limitations may force the cells to alter their metabolic states and thus, impact the efficiency of fermentation process and quality of final beer. Internal mass transfer can be optimized by adjusting the immobilization matrix size, texture and porosity. The classical dripping method, that is commonly used to produce gel beads, has a limitation in the large diameter of the produced beads, which is typically 2 to 3 mm. In recent time other techniques have been developed to produce smaller particles of equal size. The major objective of this study was to optimize the bead size by using the new technique, electrostatic droplet generation. We have obtained promising results since in this way it was possible to produce very small uniform microbeads (in the diameter range of several hundreds of micrometers to 2 mm) under stable conditions. The size of beads was strongly influenced by applied potential, needle size and electrode distance., Pri primeni imobilisanih ćelija kvasca u fermentaciji piva važno je, kako sa aspekta produktivnosti sistema, tako i sa aspekta hemijskog sastava piva, da se otpori prenosu mase supstrata i proizvoda metabolizma ćelija unutar matrice nosača svedu na najmanju moguću meru. Ovo se može postići podešavanjem veličine, teksture i poroznosti matrice nosača. Obzirom da klasična tehnika imobilizacije kvasca u matrici Ca-alginata daje čestice većih dimenzija (prečnika od 2 do 3 mm), danas je razvijeno vise različitih tehnika koje pružaju mogućnost dobijanja čestica malih dimenzija. Jedna od novih tehnika, čije su mogućnosti primene u cilju optimizacije veličine alginatnih čestica ispitivane u sklopu ovog istraživanja, je postupak elektrostatičke ekstruzije. Dobijeni rezultati su pokazali daje primenom ove tehnike moguće dobiti kontrolisanu i stabilnu proizvodnju mikro čestica uniformne veličine, čiji se prečnik kretao u rasponu od nekoliko stotina mikrometara do 2 mm, u zavisnosti od primenjene razlike potencijala, prečnika igle i rastojanja između elektroda.",
publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad",
journal = "Acta periodica technologica",
title = "Immobilization of yeast cells in small alginate beads using electrostatic droplet generation, Primena postupka elektrostatičke ekstruzije pri dobijanju alginatnih čestica malih dimenzija sa imobilisanim ćelijama pivskog kvasca",
pages = "597-591",
number = "31",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_172"
}

Nedović, V., Leskošek-Čukalović, I., Trifunović, O., Pešić, R.,& Bugarski, B.. (2000). Immobilization of yeast cells in small alginate beads using electrostatic droplet generation. in Acta periodica technologica
Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad.(31), 591-597.
https://hdl.handle.net/21.15107/rcub_agrospace_172

Nedović V, Leskošek-Čukalović I, Trifunović O, Pešić R, Bugarski B. Immobilization of yeast cells in small alginate beads using electrostatic droplet generation. in Acta periodica technologica. 2000;(31):591-597.
https://hdl.handle.net/21.15107/rcub_agrospace_172 .

Nedović, Viktor, Leskošek-Čukalović, Ida, Trifunović, Olivera, Pešić, Radojica, Bugarski, Branko, "Immobilization of yeast cells in small alginate beads using electrostatic droplet generation" in Acta periodica technologica, no. 31 (2000):591-597,
https://hdl.handle.net/21.15107/rcub_agrospace_172 .