TY  - JOUR
AU  - Milošević, Nebojša
AU  - Glišić, Ivana
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Marić, Slađana
AU  - Milošević, Tomo
AU  - Nikolić, Dragan
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S1161030123001776
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6410
AB  - Pollination and temperature are predominant factors affecting the progamic phase of sexual plant reproduction. In light of climate change and the emergence of increasing temperatures, new knowledge is necessary to encourage fruit breeding programmes to develop cultivars adapted to these changes, as well as to find the best incompatibility relationships with the aim of achieving high fruit quality and yield. This research was conducted to reveal the effect of pollination treatments and temperature on the progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars (‘Jojo’, ‘Hanita’ and ‘Katinka’) resistant/tolerant to Sharka virus using fluorescent microscopy. Reproductive parameters (flowering overlapping, pollen germination, number of pollen tubes in the upper part of the style and locule of the ovary, dynamics of pollen tubes growth and initial and final fruit set) were investigated in vivo under different pollination treatments (cross, open and self-pollination). The influence of different temperature regimes (20 °C, 23 °C and 26 °C) on the progamic phase was also observed under cross and self-pollination. The pollination treatments significantly influenced the number of pollen tubes in the style and ovary, as well as dynamics of pollen tubes in different regions of pistil. Pollen tubes showed better growth in different crossing combinations compared to open and self-pollination. Higher temperatures accelerated pollen tube growth and reduced the time they need to reach ovules. Generally, ‘Jojo’ appeared to be the best pollenizer among the examined cultivars, while ‘Katinka’ exhibited the best response of pollen tube growth to various temperature regimes. The differences observed between growth of the pollen tubes in each genotype-temperature combination revealed that certain crossing combinations were better adapted to higher temperatures so this can be taken into account as one of the adjustments to climate change. The ability of cultivars to respond to high temperatures in the progamic phase can also be helpful for breeding programs in developing new cultivars better adapted to different temperature conditions.
T2  - European Journal of Agronomy
T2  - European Journal of AgronomyEuropean Journal of Agronomy
T1  - Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus
SP  - 126909
VL  - 149
DO  - 10.1016/j.eja.2023.126909
ER  - 

@article{
author = "Milošević, Nebojša and Glišić, Ivana and Đorđević, Milena and Cerović, Radosav and Radičević, Sanja and Marić, Slađana and Milošević, Tomo and Nikolić, Dragan",
year = "2023",
abstract = "Pollination and temperature are predominant factors affecting the progamic phase of sexual plant reproduction. In light of climate change and the emergence of increasing temperatures, new knowledge is necessary to encourage fruit breeding programmes to develop cultivars adapted to these changes, as well as to find the best incompatibility relationships with the aim of achieving high fruit quality and yield. This research was conducted to reveal the effect of pollination treatments and temperature on the progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars (‘Jojo’, ‘Hanita’ and ‘Katinka’) resistant/tolerant to Sharka virus using fluorescent microscopy. Reproductive parameters (flowering overlapping, pollen germination, number of pollen tubes in the upper part of the style and locule of the ovary, dynamics of pollen tubes growth and initial and final fruit set) were investigated in vivo under different pollination treatments (cross, open and self-pollination). The influence of different temperature regimes (20 °C, 23 °C and 26 °C) on the progamic phase was also observed under cross and self-pollination. The pollination treatments significantly influenced the number of pollen tubes in the style and ovary, as well as dynamics of pollen tubes in different regions of pistil. Pollen tubes showed better growth in different crossing combinations compared to open and self-pollination. Higher temperatures accelerated pollen tube growth and reduced the time they need to reach ovules. Generally, ‘Jojo’ appeared to be the best pollenizer among the examined cultivars, while ‘Katinka’ exhibited the best response of pollen tube growth to various temperature regimes. The differences observed between growth of the pollen tubes in each genotype-temperature combination revealed that certain crossing combinations were better adapted to higher temperatures so this can be taken into account as one of the adjustments to climate change. The ability of cultivars to respond to high temperatures in the progamic phase can also be helpful for breeding programs in developing new cultivars better adapted to different temperature conditions.",
journal = "European Journal of Agronomy, European Journal of AgronomyEuropean Journal of Agronomy",
title = "Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus",
pages = "126909",
volume = "149",
doi = "10.1016/j.eja.2023.126909"
}

Milošević, N., Glišić, I., Đorđević, M., Cerović, R., Radičević, S., Marić, S., Milošević, T.,& Nikolić, D.. (2023). Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus. in European Journal of Agronomy, 149, 126909.
https://doi.org/10.1016/j.eja.2023.126909

Milošević N, Glišić I, Đorđević M, Cerović R, Radičević S, Marić S, Milošević T, Nikolić D. Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus. in European Journal of Agronomy. 2023;149:126909.
doi:10.1016/j.eja.2023.126909 .

Milošević, Nebojša, Glišić, Ivana, Đorđević, Milena, Cerović, Radosav, Radičević, Sanja, Marić, Slađana, Milošević, Tomo, Nikolić, Dragan, "Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus" in European Journal of Agronomy, 149 (2023):126909,
https://doi.org/10.1016/j.eja.2023.126909 . .

TY  - JOUR
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Nikolić, Dragan
AU  - Radičević, Sanja
AU  - Glišić, Ivana
AU  - Milošević, Nebojša
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5894
AB  - Pollen grain micromorphological characterisation of four plum (Prunus domestica L.) genotypes from two different breeding programmes (Fruit Research Institute, Serbia, and Hohenheim, Germany) was performed using scanning electron microscopy (SEM). The studied characteristics were pollen size and shape, colpus length, mesocolpium width as well as exine characteristics. All genotypes had tricolpate pollen grains and striate exine ornamentation, with more parallel longitudinal ridges. The most significant differences among the analysed genotypes concerned the length of the pollen grain and exine sculpturing. The genotypes 'Pozna Plava', 'Presenta' and 'Čačanska najbolja' had the largest pollen grains (> 57 µm), whereas 'Hanita' showed a smaller pollen size, below 54 µm. 'Pozna Plava' had perprolate pollen in the equatorial view (L/W ratio 2.06), while the other three genotypes had prolate shaped pollen. By analysing the pollen morphological characteristics of the plum varieties in this paper, primarily the shape, size, length of colpi and, most importantly, the sculpture of the exine, each variety showed a specificity which can be used together with other morphological characteristics for description and characterisation.
PB  - Academic Publishing House
T2  - Comptes Rendus de L'Academie Bulgare des Sciences
T1  - Using scanning electron microscopy to characterise plum (Prunus domestica L.) genotypes
EP  - 1397
IS  - 10
SP  - 1390
VL  - 73
DO  - 10.7546/CRABS.2020.10.08
ER  - 

@article{
author = "Đorđević, Milena and Cerović, Radosav and Nikolić, Dragan and Radičević, Sanja and Glišić, Ivana and Milošević, Nebojša",
year = "2021",
abstract = "Pollen grain micromorphological characterisation of four plum (Prunus domestica L.) genotypes from two different breeding programmes (Fruit Research Institute, Serbia, and Hohenheim, Germany) was performed using scanning electron microscopy (SEM). The studied characteristics were pollen size and shape, colpus length, mesocolpium width as well as exine characteristics. All genotypes had tricolpate pollen grains and striate exine ornamentation, with more parallel longitudinal ridges. The most significant differences among the analysed genotypes concerned the length of the pollen grain and exine sculpturing. The genotypes 'Pozna Plava', 'Presenta' and 'Čačanska najbolja' had the largest pollen grains (> 57 µm), whereas 'Hanita' showed a smaller pollen size, below 54 µm. 'Pozna Plava' had perprolate pollen in the equatorial view (L/W ratio 2.06), while the other three genotypes had prolate shaped pollen. By analysing the pollen morphological characteristics of the plum varieties in this paper, primarily the shape, size, length of colpi and, most importantly, the sculpture of the exine, each variety showed a specificity which can be used together with other morphological characteristics for description and characterisation.",
publisher = "Academic Publishing House",
journal = "Comptes Rendus de L'Academie Bulgare des Sciences",
title = "Using scanning electron microscopy to characterise plum (Prunus domestica L.) genotypes",
pages = "1397-1390",
number = "10",
volume = "73",
doi = "10.7546/CRABS.2020.10.08"
}

Đorđević, M., Cerović, R., Nikolić, D., Radičević, S., Glišić, I.,& Milošević, N.. (2021). Using scanning electron microscopy to characterise plum (Prunus domestica L.) genotypes. in Comptes Rendus de L'Academie Bulgare des Sciences
Academic Publishing House., 73(10), 1390-1397.
https://doi.org/10.7546/CRABS.2020.10.08

Đorđević M, Cerović R, Nikolić D, Radičević S, Glišić I, Milošević N. Using scanning electron microscopy to characterise plum (Prunus domestica L.) genotypes. in Comptes Rendus de L'Academie Bulgare des Sciences. 2021;73(10):1390-1397.
doi:10.7546/CRABS.2020.10.08 .

Đorđević, Milena, Cerović, Radosav, Nikolić, Dragan, Radičević, Sanja, Glišić, Ivana, Milošević, Nebojša, "Using scanning electron microscopy to characterise plum (Prunus domestica L.) genotypes" in Comptes Rendus de L'Academie Bulgare des Sciences, 73, no. 10 (2021):1390-1397,
https://doi.org/10.7546/CRABS.2020.10.08 . .

TY  - JOUR
AU  - Glišić, Ivana
AU  - Milatović, Dragan
AU  - Cerović, Radoslav
AU  - Radičević, Sanja
AU  - Đorđević, Milena
AU  - Milošević, Nebojša
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6129
AB  - Unusual growth of pollen tubes in different pollination variants (self-, cross- and open pollination) was observed in the ovary of six promising plum (Prunus domestica L.) genotypes developed at Fruit Research Institute (FRI), Čačak (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87 and 22/17/87 and cultivar ‘Nada’) in Serbia. The cross-pollination was performed using the pollen of plum cultivar ‘Čačanska Lepotica’. The occurrence of unusual pollen tubes growth in the ovary was studied over the three-year period (2009‒2011) using fluorescent microscopy method. The appearance of unusual growth of pollen tubes in the ovary of all studied genotypes during the whole examination period was recorded in the region of the obturator and the micropyle, with or without further growth of the pollen tubes to the nucellus.
Several different types of unusual growth of pollen tubes were observed, i.e. the growth of pollen tubes in opposite direction in relation to the nucellus, the curling up of pollen tubes, the branching of pollen tubes, as well as combinations of some of the above mentioned types of specific pollen tubes growth. Average values of all analyzed genotypes showed that the highest frequency of the ovaries with unusual growth of pollen tubes was determined in the open pollination variant (7.89%), followed by the cross-pollination variant (6.27%), while the smallest frequency was found in the self-pollination variant (3.12%).
PB  - Biotechnical Faculty of the University of Montenegro, Podgorica
T2  - Agriculture and Forestry
T1  - UNUSUAL GROWTH OF POLLEN TUBES IN THE OVARY OF PLUM GENOTYPES DEVELOPED AT FRUIT RESEARCH INSTITUTE ČAČAK (SERBIA)
EP  - 64
IS  - 1
SP  - 57
VL  - 65
DO  - 10.17707/AgricultForest.65.1.06
ER  - 

@article{
author = "Glišić, Ivana and Milatović, Dragan and Cerović, Radoslav and Radičević, Sanja and Đorđević, Milena and Milošević, Nebojša",
year = "2019",
abstract = "Unusual growth of pollen tubes in different pollination variants (self-, cross- and open pollination) was observed in the ovary of six promising plum (Prunus domestica L.) genotypes developed at Fruit Research Institute (FRI), Čačak (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87 and 22/17/87 and cultivar ‘Nada’) in Serbia. The cross-pollination was performed using the pollen of plum cultivar ‘Čačanska Lepotica’. The occurrence of unusual pollen tubes growth in the ovary was studied over the three-year period (2009‒2011) using fluorescent microscopy method. The appearance of unusual growth of pollen tubes in the ovary of all studied genotypes during the whole examination period was recorded in the region of the obturator and the micropyle, with or without further growth of the pollen tubes to the nucellus.
Several different types of unusual growth of pollen tubes were observed, i.e. the growth of pollen tubes in opposite direction in relation to the nucellus, the curling up of pollen tubes, the branching of pollen tubes, as well as combinations of some of the above mentioned types of specific pollen tubes growth. Average values of all analyzed genotypes showed that the highest frequency of the ovaries with unusual growth of pollen tubes was determined in the open pollination variant (7.89%), followed by the cross-pollination variant (6.27%), while the smallest frequency was found in the self-pollination variant (3.12%).",
publisher = "Biotechnical Faculty of the University of Montenegro, Podgorica",
journal = "Agriculture and Forestry",
title = "UNUSUAL GROWTH OF POLLEN TUBES IN THE OVARY OF PLUM GENOTYPES DEVELOPED AT FRUIT RESEARCH INSTITUTE ČAČAK (SERBIA)",
pages = "64-57",
number = "1",
volume = "65",
doi = "10.17707/AgricultForest.65.1.06"
}

Glišić, I., Milatović, D., Cerović, R., Radičević, S., Đorđević, M.,& Milošević, N.. (2019). UNUSUAL GROWTH OF POLLEN TUBES IN THE OVARY OF PLUM GENOTYPES DEVELOPED AT FRUIT RESEARCH INSTITUTE ČAČAK (SERBIA). in Agriculture and Forestry
Biotechnical Faculty of the University of Montenegro, Podgorica., 65(1), 57-64.
https://doi.org/10.17707/AgricultForest.65.1.06

Glišić I, Milatović D, Cerović R, Radičević S, Đorđević M, Milošević N. UNUSUAL GROWTH OF POLLEN TUBES IN THE OVARY OF PLUM GENOTYPES DEVELOPED AT FRUIT RESEARCH INSTITUTE ČAČAK (SERBIA). in Agriculture and Forestry. 2019;65(1):57-64.
doi:10.17707/AgricultForest.65.1.06 .

Glišić, Ivana, Milatović, Dragan, Cerović, Radoslav, Radičević, Sanja, Đorđević, Milena, Milošević, Nebojša, "UNUSUAL GROWTH OF POLLEN TUBES IN THE OVARY OF PLUM GENOTYPES DEVELOPED AT FRUIT RESEARCH INSTITUTE ČAČAK (SERBIA)" in Agriculture and Forestry, 65, no. 1 (2019):57-64,
https://doi.org/10.17707/AgricultForest.65.1.06 . .

TY  - CONF
AU  - Glišić, Ivana
AU  - Milatović, Dragan
AU  - Milošević, Nebojša
AU  - Lukić, Milan
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5793
AB  - This paper presents the results of a three-year study of ripening time and major physical and chemical properties of fruits of six promising plum (Prunus domestica L.) hybrids created at the Fruit Research Institute, Čačak. One of these hybrids was released as a new cultivar and was named ‘Nada’. The cultivar ‘Čačanska Lepotica’ served as the standard. The genotypes under study ripened between 29 July (hybrid 38/62/70) and 2 September (hybrid 22/17/87). An elongated fruit shape, a yellowish-green flesh colour and a firm fruit prevailed, whereas violet blue and dark blue skin colours were equally present. Fruit weight ranged from 26.96±0.68 g (22/17/87) to 56.92±2.87 g (38/62/70), with higher values obtained in 38/62/70 and the new cv.‘Nada’, compared to the standard. The highest flesh/stone ratio was found in hybrid 38/62/70 (96.91±0.19%), which was the only significantly higher result compared to the control cultivar. The highest soluble solids content was measured in hybrid 22/17/87 (17.01±0.96%), and the lowest in hybrid 38/62/70 (11.39±0.44%). Compared to the standard, higher values of soluble solids were exhibited by ‘Nada’ and hybrids 32/21/87, 34/41/87 and 22/17/87. Total acid levels were highest in hybrid 32/21/87 (1.42±0.05%), and lowest in ‘Nada’ (0.35±0.02%). Compared to the standard, higher levels were found in fruits of hybrids 38/62/70, IV/63/81 and 32/21/87. The ratio of soluble solids to total acids was highest in ‘Nada’ (43.72±1.47) and lowest in hybrid 32/21/87 (10.25±0.58). Compared to the control cultivar, higher values of this parameter were observed in ‘Nada’, and hybrids 34/41/87 and 22/17/87. The results of this research suggest that ‘Nada’ can be singled out among the tested genotypes as having the best combination of positive traits.
PB  - Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina
C3  - Sixth International Scientific Agricultural Symposium „Agrosym 2015“
T1  - Biological and pomological properties of promising plum hybrids created at the Fruit Research Institute, Čačak
EP  - 429
SP  - 424
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5793
ER  - 

@conference{
author = "Glišić, Ivana and Milatović, Dragan and Milošević, Nebojša and Lukić, Milan",
year = "2015",
abstract = "This paper presents the results of a three-year study of ripening time and major physical and chemical properties of fruits of six promising plum (Prunus domestica L.) hybrids created at the Fruit Research Institute, Čačak. One of these hybrids was released as a new cultivar and was named ‘Nada’. The cultivar ‘Čačanska Lepotica’ served as the standard. The genotypes under study ripened between 29 July (hybrid 38/62/70) and 2 September (hybrid 22/17/87). An elongated fruit shape, a yellowish-green flesh colour and a firm fruit prevailed, whereas violet blue and dark blue skin colours were equally present. Fruit weight ranged from 26.96±0.68 g (22/17/87) to 56.92±2.87 g (38/62/70), with higher values obtained in 38/62/70 and the new cv.‘Nada’, compared to the standard. The highest flesh/stone ratio was found in hybrid 38/62/70 (96.91±0.19%), which was the only significantly higher result compared to the control cultivar. The highest soluble solids content was measured in hybrid 22/17/87 (17.01±0.96%), and the lowest in hybrid 38/62/70 (11.39±0.44%). Compared to the standard, higher values of soluble solids were exhibited by ‘Nada’ and hybrids 32/21/87, 34/41/87 and 22/17/87. Total acid levels were highest in hybrid 32/21/87 (1.42±0.05%), and lowest in ‘Nada’ (0.35±0.02%). Compared to the standard, higher levels were found in fruits of hybrids 38/62/70, IV/63/81 and 32/21/87. The ratio of soluble solids to total acids was highest in ‘Nada’ (43.72±1.47) and lowest in hybrid 32/21/87 (10.25±0.58). Compared to the control cultivar, higher values of this parameter were observed in ‘Nada’, and hybrids 34/41/87 and 22/17/87. The results of this research suggest that ‘Nada’ can be singled out among the tested genotypes as having the best combination of positive traits.",
publisher = "Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina",
journal = "Sixth International Scientific Agricultural Symposium „Agrosym 2015“",
title = "Biological and pomological properties of promising plum hybrids created at the Fruit Research Institute, Čačak",
pages = "429-424",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5793"
}

Glišić, I., Milatović, D., Milošević, N.,& Lukić, M.. (2015). Biological and pomological properties of promising plum hybrids created at the Fruit Research Institute, Čačak. in Sixth International Scientific Agricultural Symposium „Agrosym 2015“
Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina., 424-429.
https://hdl.handle.net/21.15107/rcub_agrospace_5793

Glišić I, Milatović D, Milošević N, Lukić M. Biological and pomological properties of promising plum hybrids created at the Fruit Research Institute, Čačak. in Sixth International Scientific Agricultural Symposium „Agrosym 2015“. 2015;:424-429.
https://hdl.handle.net/21.15107/rcub_agrospace_5793 .

Glišić, Ivana, Milatović, Dragan, Milošević, Nebojša, Lukić, Milan, "Biological and pomological properties of promising plum hybrids created at the Fruit Research Institute, Čačak" in Sixth International Scientific Agricultural Symposium „Agrosym 2015“ (2015):424-429,
https://hdl.handle.net/21.15107/rcub_agrospace_5793 .