TY  - JOUR
AU  - Krstić, Đurđa
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Fotirić Akšić, Milica
AU  - Đorđević, Boban
AU  - Milojković-Opsenica, Dušanka
AU  - Andrić, Filip
AU  - Trifković, Jelena
PY  - 2022
UR  - https://hrcak.srce.hr/clanak/412101
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6203
AB  - Research background. Considering the importance of consumption of berry fruits with
proven health-beneficial properties and difficulties in quality control of products of specific botanical and geographic origin, a fingerprint method was developed, based on advanced data analysis (pattern recognition, classification), in order to relate the variability
of nutrients in the selected cultivars to primary metabolite profile.
Experimental approach. Forty-five samples of genuine berry fruit cultivars (strawberry,
raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry
and goji berry) were characterized according to chromatographic profiles of primary metabolites (sugars, lipids and fatty acids) obtained by three chromatographic techniques
(high-performance thin-layer chromatography, gas chromatography coupled to mass
spectrometry, and high-performance anion-exchange chromatography with pulsed amperometric detection).
Results and conclusions. Comprehensive analysis allowed monitoring and identification
of metabolites belonging to polar lipids, mono-, di- and triacylglycerols, free fatty acids,
free sterols, sterol esters, mono- to heptasaccharides and sugar alcohols. Chemical fingerprint of berry seeds showed the uniformity of primary metabolites within each fruit species, but revealed differences depending on the botanical origin. All three chromatographic methods provided a discriminative, informative and predictive metabolomics
methodology, which proved to be useful for chemotaxonomic classification.
Novelty and scientific contribution. A novel methodology for the identification of bioactive compounds from primary metabolites of natural products was described. The proposed untargeted metabolite profiling approach could be used in the future as a routine
method for tracing of novel bioactive compounds. The knowledge of metabolite composition obtained in this study can provide a better assessment of genotypic and phenotypic differences between berry fruit species and varieties, and could contribute to the development of new breeding programs.
T2  - Food Technology and Biotechnology
T2  - Food Technology and Biotechnology
T1  - Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits
EP  - 417
IS  - 3
SP  - 406
VL  - 60
DO  - 10.17113/ftb.60.03.22.7505
ER  - 

@article{
author = "Krstić, Đurđa and Tosti, Tomislav and Đurović, Saša and Fotirić Akšić, Milica and Đorđević, Boban and Milojković-Opsenica, Dušanka and Andrić, Filip and Trifković, Jelena",
year = "2022",
abstract = "Research background. Considering the importance of consumption of berry fruits with
proven health-beneficial properties and difficulties in quality control of products of specific botanical and geographic origin, a fingerprint method was developed, based on advanced data analysis (pattern recognition, classification), in order to relate the variability
of nutrients in the selected cultivars to primary metabolite profile.
Experimental approach. Forty-five samples of genuine berry fruit cultivars (strawberry,
raspberry, blackberry, black currant, blueberry, gooseberry, chokeberry, cape gooseberry
and goji berry) were characterized according to chromatographic profiles of primary metabolites (sugars, lipids and fatty acids) obtained by three chromatographic techniques
(high-performance thin-layer chromatography, gas chromatography coupled to mass
spectrometry, and high-performance anion-exchange chromatography with pulsed amperometric detection).
Results and conclusions. Comprehensive analysis allowed monitoring and identification
of metabolites belonging to polar lipids, mono-, di- and triacylglycerols, free fatty acids,
free sterols, sterol esters, mono- to heptasaccharides and sugar alcohols. Chemical fingerprint of berry seeds showed the uniformity of primary metabolites within each fruit species, but revealed differences depending on the botanical origin. All three chromatographic methods provided a discriminative, informative and predictive metabolomics
methodology, which proved to be useful for chemotaxonomic classification.
Novelty and scientific contribution. A novel methodology for the identification of bioactive compounds from primary metabolites of natural products was described. The proposed untargeted metabolite profiling approach could be used in the future as a routine
method for tracing of novel bioactive compounds. The knowledge of metabolite composition obtained in this study can provide a better assessment of genotypic and phenotypic differences between berry fruit species and varieties, and could contribute to the development of new breeding programs.",
journal = "Food Technology and Biotechnology, Food Technology and Biotechnology",
title = "Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits",
pages = "417-406",
number = "3",
volume = "60",
doi = "10.17113/ftb.60.03.22.7505"
}

Krstić, Đ., Tosti, T., Đurović, S., Fotirić Akšić, M., Đorđević, B., Milojković-Opsenica, D., Andrić, F.,& Trifković, J.. (2022). Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits. in Food Technology and Biotechnology, 60(3), 406-417.
https://doi.org/10.17113/ftb.60.03.22.7505

Krstić Đ, Tosti T, Đurović S, Fotirić Akšić M, Đorđević B, Milojković-Opsenica D, Andrić F, Trifković J. Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits. in Food Technology and Biotechnology. 2022;60(3):406-417.
doi:10.17113/ftb.60.03.22.7505 .

Krstić, Đurđa, Tosti, Tomislav, Đurović, Saša, Fotirić Akšić, Milica, Đorđević, Boban, Milojković-Opsenica, Dušanka, Andrić, Filip, Trifković, Jelena, "Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits" in Food Technology and Biotechnology, 60, no. 3 (2022):406-417,
https://doi.org/10.17113/ftb.60.03.22.7505 . .

TY  - JOUR
AU  - Krstić, Đurđa
AU  - Vukojević, Vesna
AU  - Mutić, Jelena
AU  - Fotirić-Akšić, Milica
AU  - Ličina, Vlado
AU  - Milojković-Opsenica, Dušanka M.
AU  - Trifković, Jelena
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5025
AB  - BACKGROUND:  The compositional, functional, and nutritional properties of fruits are important for defining their quality. Fruit seeds should be better exploited as they are also considered to be a good source of bioactive components. Twenty macro, micro, and trace elements were identified and quantified in the seeds of 70 genuine wild and cultivated fruit species/cultivars by inductively coupled plasma atomic emission spectrometry and inductively coupled plasma mass spectrometry. Sophisticated chemometric techniques were also used to establish criteria for the classification of the analyzed samples. RESULTS Calcium and P were the most abundant elements, followed by K and Na. The content of microelements and trace elements differed among the different cultivars/genotypes. The content of Ba, Pb, and Sr was significantly higher in wild fruits, whereas Fe, Mg, Mn, Ni and Zn content was higher in cultivated fruits. CONCLUSION All of the statistical procedures that were used - Kruskal-Wallis, Mann-Whitney U-test, and principal component analysis (PCA) - confirm a unique set of parameters that could be used as phytochemical biomarkers to differentiate fruit-seed samples belonging to different cultivars/genotypes according to their botanical origin. This kind of investigation may contribute to intercultivar/genetic discrimination and may enhance the possibilities of acquiring a valuable authenticity factor.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Distribution of elements in seeds of some wild and cultivated fruits. Nutrition and authenticity aspects
EP  - 554
IS  - 2
SP  - 546
VL  - 99
DO  - 10.1002/jsfa.9213
ER  - 

@article{
author = "Krstić, Đurđa and Vukojević, Vesna and Mutić, Jelena and Fotirić-Akšić, Milica and Ličina, Vlado and Milojković-Opsenica, Dušanka M. and Trifković, Jelena",
year = "2019",
abstract = "BACKGROUND:  The compositional, functional, and nutritional properties of fruits are important for defining their quality. Fruit seeds should be better exploited as they are also considered to be a good source of bioactive components. Twenty macro, micro, and trace elements were identified and quantified in the seeds of 70 genuine wild and cultivated fruit species/cultivars by inductively coupled plasma atomic emission spectrometry and inductively coupled plasma mass spectrometry. Sophisticated chemometric techniques were also used to establish criteria for the classification of the analyzed samples. RESULTS Calcium and P were the most abundant elements, followed by K and Na. The content of microelements and trace elements differed among the different cultivars/genotypes. The content of Ba, Pb, and Sr was significantly higher in wild fruits, whereas Fe, Mg, Mn, Ni and Zn content was higher in cultivated fruits. CONCLUSION All of the statistical procedures that were used - Kruskal-Wallis, Mann-Whitney U-test, and principal component analysis (PCA) - confirm a unique set of parameters that could be used as phytochemical biomarkers to differentiate fruit-seed samples belonging to different cultivars/genotypes according to their botanical origin. This kind of investigation may contribute to intercultivar/genetic discrimination and may enhance the possibilities of acquiring a valuable authenticity factor.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Distribution of elements in seeds of some wild and cultivated fruits. Nutrition and authenticity aspects",
pages = "554-546",
number = "2",
volume = "99",
doi = "10.1002/jsfa.9213"
}

Krstić, Đ., Vukojević, V., Mutić, J., Fotirić-Akšić, M., Ličina, V., Milojković-Opsenica, D. M.,& Trifković, J.. (2019). Distribution of elements in seeds of some wild and cultivated fruits. Nutrition and authenticity aspects. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 99(2), 546-554.
https://doi.org/10.1002/jsfa.9213

Krstić Đ, Vukojević V, Mutić J, Fotirić-Akšić M, Ličina V, Milojković-Opsenica DM, Trifković J. Distribution of elements in seeds of some wild and cultivated fruits. Nutrition and authenticity aspects. in Journal of the Science of Food and Agriculture. 2019;99(2):546-554.
doi:10.1002/jsfa.9213 .

Krstić, Đurđa, Vukojević, Vesna, Mutić, Jelena, Fotirić-Akšić, Milica, Ličina, Vlado, Milojković-Opsenica, Dušanka M., Trifković, Jelena, "Distribution of elements in seeds of some wild and cultivated fruits. Nutrition and authenticity aspects" in Journal of the Science of Food and Agriculture, 99, no. 2 (2019):546-554,
https://doi.org/10.1002/jsfa.9213 . .

TY  - JOUR
AU  - Mesarović, Jelena
AU  - Trifković, Jelena
AU  - Tosti, Tomislav
AU  - Fotirić-Akšić, Milica
AU  - Milatović, Dragan
AU  - Ličina, Vlado
AU  - Milojković-Opsenica, Dušanka M.
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4650
AB  - Apricot seeds could be obtained as a byproduct in different juice or conserve producing industries. Disposal of large amount of apricot seeds is wasting of potentially precious sources of phytochemicals. This study encompassed apricot cultivars that have different origin and flowering time with the aim of providing valuable information about the sugar content in its kernels. High-performance anion-exchange chromatography with pulsed amperometric detection was used for the determination of 22 sugars in the kernels of 70 apricot cultivars. The most common sugars in the apricot kernels are sucrose, fructose, and glucose whose ratio proved to be ripening time dependent (1:1:1 in the early, 1:2:3 in medium and 1:3:3 in the late cultivars). The kernels of the apricot with shorter vegetation period had the highest sum of glucose and fructose compared to other groups. Other sugar components were present in different levels as minor constituents depending on the ripening time. Also, most common sugars and some low-level sugars were strongly correlated to each other, indicating the normal metabolic carbohydrate pathway. To understand the distribution modes of sugars, a principal component analysis was performed.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Relationship between ripening time and sugar content of apricot (Prunus armeniaca L.) kernels
IS  - 8
VL  - 40
DO  - 10.1007/s11738-018-2731-7
ER  - 

@article{
author = "Mesarović, Jelena and Trifković, Jelena and Tosti, Tomislav and Fotirić-Akšić, Milica and Milatović, Dragan and Ličina, Vlado and Milojković-Opsenica, Dušanka M.",
year = "2018",
abstract = "Apricot seeds could be obtained as a byproduct in different juice or conserve producing industries. Disposal of large amount of apricot seeds is wasting of potentially precious sources of phytochemicals. This study encompassed apricot cultivars that have different origin and flowering time with the aim of providing valuable information about the sugar content in its kernels. High-performance anion-exchange chromatography with pulsed amperometric detection was used for the determination of 22 sugars in the kernels of 70 apricot cultivars. The most common sugars in the apricot kernels are sucrose, fructose, and glucose whose ratio proved to be ripening time dependent (1:1:1 in the early, 1:2:3 in medium and 1:3:3 in the late cultivars). The kernels of the apricot with shorter vegetation period had the highest sum of glucose and fructose compared to other groups. Other sugar components were present in different levels as minor constituents depending on the ripening time. Also, most common sugars and some low-level sugars were strongly correlated to each other, indicating the normal metabolic carbohydrate pathway. To understand the distribution modes of sugars, a principal component analysis was performed.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Relationship between ripening time and sugar content of apricot (Prunus armeniaca L.) kernels",
number = "8",
volume = "40",
doi = "10.1007/s11738-018-2731-7"
}

Mesarović, J., Trifković, J., Tosti, T., Fotirić-Akšić, M., Milatović, D., Ličina, V.,& Milojković-Opsenica, D. M.. (2018). Relationship between ripening time and sugar content of apricot (Prunus armeniaca L.) kernels. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 40(8).
https://doi.org/10.1007/s11738-018-2731-7

Mesarović J, Trifković J, Tosti T, Fotirić-Akšić M, Milatović D, Ličina V, Milojković-Opsenica DM. Relationship between ripening time and sugar content of apricot (Prunus armeniaca L.) kernels. in Acta Physiologiae Plantarum. 2018;40(8).
doi:10.1007/s11738-018-2731-7 .

Mesarović, Jelena, Trifković, Jelena, Tosti, Tomislav, Fotirić-Akšić, Milica, Milatović, Dragan, Ličina, Vlado, Milojković-Opsenica, Dušanka M., "Relationship between ripening time and sugar content of apricot (Prunus armeniaca L.) kernels" in Acta Physiologiae Plantarum, 40, no. 8 (2018),
https://doi.org/10.1007/s11738-018-2731-7 . .

TY  - JOUR
AU  - Lazarević, Kristina B.
AU  - Trifković, Jelena dj.
AU  - Andrić, Filip Lj.
AU  - Tešić, Živoslav
AU  - Andjelković, Ivan B.
AU  - Radović, Dejan I.
AU  - Nedić, Nebojša
AU  - Milojković-Opsenica, Dušanka M.
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3091
AB  - Multifloral honey was characterized in regards mineral to composition, sugar content and basic physicochemical properties. A total of 164 honey samples were collected from different regions of Serbia during the harvesting season 2009. Univariate data analysis (descriptive statistics and analysis of variance), geographic information system and pattern recognition methods (principal component analysis and cluster analysis) were utilized in order to identify the geographical origin of honey. The content of Mg, K, and Cu, electrical conductivity and optical rotation were established as useful indicators in tracing regional differences between honey samples. Samples originating from Zlatibor region were clearly distinguished from those from the rest of Serbia, showing higher K and Mg contents, as well as higher values of optical rotation, electrical conductivity, and free acidity. The influence of the soil composition, and climate conditions, as well as the presence of particular flora on the honey composition is emphasized. The modeling of the geographic origin of honey was attempted by means of linear discriminant analysis. .
AB  - Karakterizacija polifloralnih medova u pogledu mineralnog sastava, sadržaja šećera i osnovnih fizičko-hemijskih parametara izvršena je na osnovu analize 164 uzorka iz različitih regiona Srbije sakupljenih tokom 2009. godine. Univarijantna analiza podataka (deskriptivna statistika i analiza varijanse), zajedno sa geografskim informacionim sistemom i multivarijantnom hemometrijskom analizom (analiza glavnih komponenata i klasterska analiza) primenjene su u cilju određivanja geografskog porekla meda. Utvrđeno je da su sadržaj Mg, K i Cu, električna provodljivost i optička rotacija parametri koji ukazuju na razlike u regionalnom poreklu meda. Uzorci koji potiču iz zlatiborskog regiona su se jasno razdvojili od uzoraka iz ostalih delova Srbije na osnovu većeg sadržaja K i Mg, kao i većih vrednosti optičke rotacije, električne provodljivosti i slobodne kiselosti. Ukazano je na uticaj sastava zemljišta, klimatskih uslova i postojanja endemičnih vrsta na sastav meda. Modelovanje geografskog porekla meda je urađeno primenom linearne diskriminantne analize. .
PB  - Srpsko hemijsko društvo, Beograd
T2  - JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
T1  - Quality parameters and pattern recognition methods as a tool in tracing the regional origin of multifloral honey
T1  - Određivanje geografskog porekla polifloralnih medova pomoću parametara kvaliteta i metoda prepoznavanja obrazaca
EP  - 1892
IS  - 12
SP  - 1875
VL  - 78
DO  - 10.2298/JSC130701099L
ER  - 

@article{
author = "Lazarević, Kristina B. and Trifković, Jelena dj. and Andrić, Filip Lj. and Tešić, Živoslav and Andjelković, Ivan B. and Radović, Dejan I. and Nedić, Nebojša and Milojković-Opsenica, Dušanka M.",
year = "2013",
abstract = "Multifloral honey was characterized in regards mineral to composition, sugar content and basic physicochemical properties. A total of 164 honey samples were collected from different regions of Serbia during the harvesting season 2009. Univariate data analysis (descriptive statistics and analysis of variance), geographic information system and pattern recognition methods (principal component analysis and cluster analysis) were utilized in order to identify the geographical origin of honey. The content of Mg, K, and Cu, electrical conductivity and optical rotation were established as useful indicators in tracing regional differences between honey samples. Samples originating from Zlatibor region were clearly distinguished from those from the rest of Serbia, showing higher K and Mg contents, as well as higher values of optical rotation, electrical conductivity, and free acidity. The influence of the soil composition, and climate conditions, as well as the presence of particular flora on the honey composition is emphasized. The modeling of the geographic origin of honey was attempted by means of linear discriminant analysis. ., Karakterizacija polifloralnih medova u pogledu mineralnog sastava, sadržaja šećera i osnovnih fizičko-hemijskih parametara izvršena je na osnovu analize 164 uzorka iz različitih regiona Srbije sakupljenih tokom 2009. godine. Univarijantna analiza podataka (deskriptivna statistika i analiza varijanse), zajedno sa geografskim informacionim sistemom i multivarijantnom hemometrijskom analizom (analiza glavnih komponenata i klasterska analiza) primenjene su u cilju određivanja geografskog porekla meda. Utvrđeno je da su sadržaj Mg, K i Cu, električna provodljivost i optička rotacija parametri koji ukazuju na razlike u regionalnom poreklu meda. Uzorci koji potiču iz zlatiborskog regiona su se jasno razdvojili od uzoraka iz ostalih delova Srbije na osnovu većeg sadržaja K i Mg, kao i većih vrednosti optičke rotacije, električne provodljivosti i slobodne kiselosti. Ukazano je na uticaj sastava zemljišta, klimatskih uslova i postojanja endemičnih vrsta na sastav meda. Modelovanje geografskog porekla meda je urađeno primenom linearne diskriminantne analize. .",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "JOURNAL OF THE SERBIAN CHEMICAL SOCIETY",
title = "Quality parameters and pattern recognition methods as a tool in tracing the regional origin of multifloral honey, Određivanje geografskog porekla polifloralnih medova pomoću parametara kvaliteta i metoda prepoznavanja obrazaca",
pages = "1892-1875",
number = "12",
volume = "78",
doi = "10.2298/JSC130701099L"
}

Lazarević, K. B., Trifković, J. dj., Andrić, F. Lj., Tešić, Ž., Andjelković, I. B., Radović, D. I., Nedić, N.,& Milojković-Opsenica, D. M.. (2013). Quality parameters and pattern recognition methods as a tool in tracing the regional origin of multifloral honey. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY
Srpsko hemijsko društvo, Beograd., 78(12), 1875-1892.
https://doi.org/10.2298/JSC130701099L

Lazarević KB, Trifković JD, Andrić FL, Tešić Ž, Andjelković IB, Radović DI, Nedić N, Milojković-Opsenica DM. Quality parameters and pattern recognition methods as a tool in tracing the regional origin of multifloral honey. in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY. 2013;78(12):1875-1892.
doi:10.2298/JSC130701099L .

Lazarević, Kristina B., Trifković, Jelena dj., Andrić, Filip Lj., Tešić, Živoslav, Andjelković, Ivan B., Radović, Dejan I., Nedić, Nebojša, Milojković-Opsenica, Dušanka M., "Quality parameters and pattern recognition methods as a tool in tracing the regional origin of multifloral honey" in JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 78, no. 12 (2013):1875-1892,
https://doi.org/10.2298/JSC130701099L . .