TY  - JOUR
AU  - Ðorđević, Milena
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
AU  - Glišić, Ivana
AU  - Milošević, Nebojša
AU  - Marić, Slađana
AU  - Radičević, Sanja
AU  - Fotirić Akšić, Milica
AU  - Meland, Mekjell
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6146
AB  - A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−20, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
T2  - Horticulturae
T2  - Horticulturae
T1  - In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070616
ER  - 

@article{
author = "Ðorđević, Milena and Vujović, Tatjana and Cerović, Radosav and Glišić, Ivana and Milošević, Nebojša and Marić, Slađana and Radičević, Sanja and Fotirić Akšić, Milica and Meland, Mekjell",
year = "2022",
abstract = "A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−20, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
journal = "Horticulturae, Horticulturae",
title = "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070616"
}

Ðorđević, M., Vujović, T., Cerović, R., Glišić, I., Milošević, N., Marić, S., Radičević, S., Fotirić Akšić, M.,& Meland, M.. (2022). In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods. in Horticulturae, 8(7).
https://doi.org/10.3390/horticulturae8070616

Ðorđević M, Vujović T, Cerović R, Glišić I, Milošević N, Marić S, Radičević S, Fotirić Akšić M, Meland M. In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods. in Horticulturae. 2022;8(7).
doi:10.3390/horticulturae8070616 .

Ðorđević, Milena, Vujović, Tatjana, Cerović, Radosav, Glišić, Ivana, Milošević, Nebojša, Marić, Slađana, Radičević, Sanja, Fotirić Akšić, Milica, Meland, Mekjell, "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods" in Horticulturae, 8, no. 7 (2022),
https://doi.org/10.3390/horticulturae8070616 . .