TY  - JOUR
AU  - Zhu, Dong
AU  - Liu, Yanlin
AU  - Jin, Man
AU  - Chen, Guanxing
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5055
AB  - Expansins are a group of plant cell wall loosening proteins that play important roles in plant growth and development. In this study, we performed the first study on the molecular characterization, transcriptional expression and functional properties of two wheat expansin genes TaEXPA2 and TaEXPB1. The results indicated that TaEXPA2 and TaEXPB1 genes had typical structural features of plant expansin gene family. As a member of alpha-expansins, TaEXPA2 is closely related to rice OsEXPA17 while the beta-expansin member TaEXPB1 has closely phylogenetic relationships with rice OsEXPAB4. The genetic transformation to Arabidopsis showed that both TaEXPA2 and TaEXPB1 were located in cell wall and highly expressed in roots, leaves and seeds. Overexpression of TaEXPA2 and TaEXPB1 genes showed similar functions, causing rapid root elongation, early bolting, and increases in leaves number, rosette diameter and stems length. These results demonstrated that wheat expansin genes TaEXPA1 and TaEXPB2 can enhance plant growth and development.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Expression and function analysis of wheat expasin genes expa2 and expb1
EP  - 274
IS  - 1
SP  - 261
VL  - 51
DO  - 10.2298/GENSR1901261Z
ER  - 

@article{
author = "Zhu, Dong and Liu, Yanlin and Jin, Man and Chen, Guanxing and Prodanović, Slaven and Yan, Yueming",
year = "2019",
abstract = "Expansins are a group of plant cell wall loosening proteins that play important roles in plant growth and development. In this study, we performed the first study on the molecular characterization, transcriptional expression and functional properties of two wheat expansin genes TaEXPA2 and TaEXPB1. The results indicated that TaEXPA2 and TaEXPB1 genes had typical structural features of plant expansin gene family. As a member of alpha-expansins, TaEXPA2 is closely related to rice OsEXPA17 while the beta-expansin member TaEXPB1 has closely phylogenetic relationships with rice OsEXPAB4. The genetic transformation to Arabidopsis showed that both TaEXPA2 and TaEXPB1 were located in cell wall and highly expressed in roots, leaves and seeds. Overexpression of TaEXPA2 and TaEXPB1 genes showed similar functions, causing rapid root elongation, early bolting, and increases in leaves number, rosette diameter and stems length. These results demonstrated that wheat expansin genes TaEXPA1 and TaEXPB2 can enhance plant growth and development.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Expression and function analysis of wheat expasin genes expa2 and expb1",
pages = "274-261",
number = "1",
volume = "51",
doi = "10.2298/GENSR1901261Z"
}

Zhu, D., Liu, Y., Jin, M., Chen, G., Prodanović, S.,& Yan, Y.. (2019). Expression and function analysis of wheat expasin genes expa2 and expb1. in Genetika
Društvo genetičara Srbije, Beograd., 51(1), 261-274.
https://doi.org/10.2298/GENSR1901261Z

Zhu D, Liu Y, Jin M, Chen G, Prodanović S, Yan Y. Expression and function analysis of wheat expasin genes expa2 and expb1. in Genetika. 2019;51(1):261-274.
doi:10.2298/GENSR1901261Z .

Zhu, Dong, Liu, Yanlin, Jin, Man, Chen, Guanxing, Prodanović, Slaven, Yan, Yueming, "Expression and function analysis of wheat expasin genes expa2 and expb1" in Genetika, 51, no. 1 (2019):261-274,
https://doi.org/10.2298/GENSR1901261Z . .

TY  - JOUR
AU  - Wang, Wanqing
AU  - Wang, Ke
AU  - Chen, Xi
AU  - Prodanović, Slaven
AU  - Li, Xiaohui
AU  - Ye, Xingguo
AU  - Yan, Yueming
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4648
AB  - Gliadins, as the major components of wheat storage proteins, determine the extensibility properties of dough and have important effects on flour processing quality. Wheat related species carries potential storage protein gene resources for quality improvement. In this study, we isolated and characterized the first complete omega-gliadin gene Omega-AS from Aegilops speltoides L. (2n = 2x = 14, SS) by allelic-specific PCR and investigated its phylogenetic relationships among Triticum and Aegilops species. Molecular structure showed that Omega-AS gene consisted of 1122 bp encoding 373 amino acid residues with deduced molecular mass 41379.21 Da. Omega-AS gene was exceptionally rich in prolines and glutamines with fewer methionine and no cysteine. Sequence characterization and epitope analysis showed that three epitopes QQPIPVQPQQ, TQPQQPTPIQ and IQPQQPFPQQ were absent in Omega-AS gene encoded protein, indicating its potential value for wheat quality improvement with less toxic, or no toxic peptides. Phylogenetic analysis revealed that Omega-AS was closely related to gliadin genes of wheat and related species and its divergence from bread wheat was more recently (less than 1.243 MYA). Heterologous expression showed that Omega-AS gene could successfully express with a high level in E. coli under the control of T-7 promoter. The transcription expression pattern of Omega AS gene during grain development detected by qRT-PCR revealed that the highest expression level occurred at 17 days post anthesis.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.
EP  - 517
IS  - 2
SP  - 503
VL  - 50
DO  - 10.2298/GENSR1802503W
ER  - 

@article{
author = "Wang, Wanqing and Wang, Ke and Chen, Xi and Prodanović, Slaven and Li, Xiaohui and Ye, Xingguo and Yan, Yueming",
year = "2018",
abstract = "Gliadins, as the major components of wheat storage proteins, determine the extensibility properties of dough and have important effects on flour processing quality. Wheat related species carries potential storage protein gene resources for quality improvement. In this study, we isolated and characterized the first complete omega-gliadin gene Omega-AS from Aegilops speltoides L. (2n = 2x = 14, SS) by allelic-specific PCR and investigated its phylogenetic relationships among Triticum and Aegilops species. Molecular structure showed that Omega-AS gene consisted of 1122 bp encoding 373 amino acid residues with deduced molecular mass 41379.21 Da. Omega-AS gene was exceptionally rich in prolines and glutamines with fewer methionine and no cysteine. Sequence characterization and epitope analysis showed that three epitopes QQPIPVQPQQ, TQPQQPTPIQ and IQPQQPFPQQ were absent in Omega-AS gene encoded protein, indicating its potential value for wheat quality improvement with less toxic, or no toxic peptides. Phylogenetic analysis revealed that Omega-AS was closely related to gliadin genes of wheat and related species and its divergence from bread wheat was more recently (less than 1.243 MYA). Heterologous expression showed that Omega-AS gene could successfully express with a high level in E. coli under the control of T-7 promoter. The transcription expression pattern of Omega AS gene during grain development detected by qRT-PCR revealed that the highest expression level occurred at 17 days post anthesis.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.",
pages = "517-503",
number = "2",
volume = "50",
doi = "10.2298/GENSR1802503W"
}

Wang, W., Wang, K., Chen, X., Prodanović, S., Li, X., Ye, X.,& Yan, Y.. (2018). Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.. in Genetika
Društvo genetičara Srbije, Beograd., 50(2), 503-517.
https://doi.org/10.2298/GENSR1802503W

Wang W, Wang K, Chen X, Prodanović S, Li X, Ye X, Yan Y. Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l.. in Genetika. 2018;50(2):503-517.
doi:10.2298/GENSR1802503W .

Wang, Wanqing, Wang, Ke, Chen, Xi, Prodanović, Slaven, Li, Xiaohui, Ye, Xingguo, Yan, Yueming, "Molecular characterization and phylogenetic analysis of one omega-gliadin gene from aegilops speltoides l." in Genetika, 50, no. 2 (2018):503-517,
https://doi.org/10.2298/GENSR1802503W . .

TY  - JOUR
AU  - Wu, Jisu
AU  - Lu, Xiaobin
AU  - Yu, Zitong
AU  - Han, Caixia
AU  - Li, Xiaohui
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4407
AB  - Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and gliadins through intermolecular disulfide bond, confer dough viscoelasticity and wheat processing quality. Glutenins consist of high and low molecular weight glutenin subunits (HMW-GS, LMW-GS) encoded by Glu-1 and Glu-3 loci, respectively. The allelic variations at both loci have important effects on GMP content and breadmaking quality. In this study, GMP extraction and size-exclusion high performance liquid chromatography (SE-HPLC) separation were optimized, and then applied to investigate the effects of Glu-1 and Glu-3 allelic variations on GMP content using different wheat genotypes, chromosome substitution lines and near-isogenic lines (NILs). The results showed that the optimized GMP extraction and SE-HPLC protocol could obtain a reproducible separation and reliable quantitation of GMP content with small samples. The allelic variations at Glu-1 and Glu-3 were closely related to GMP content. Particularly, Glu-DId encoded 1Dx5+1Dy10 subunits, Glu-A3a and Glu-B3h encoded an abundant LMW-B subunit respectively had positive effects on GMP content and breadmaking quality. The introgress of HMW-GS and LMW-GS into bread wheat from related genomes could significantly increase GMP content, indicating that wheat related species has potential gene resources for breadmaking quality improvement. Our results demonstrated that SE-HPLC could serve as an effective tool for rapid separation and quantitation of GMPs and had potential application for gluten quality screening in the early generations during wheat quality improvement program.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc)
EP  - 691
IS  - 2
SP  - 677
VL  - 49
DO  - 10.2298/GENSR1702677W
ER  - 

@article{
author = "Wu, Jisu and Lu, Xiaobin and Yu, Zitong and Han, Caixia and Li, Xiaohui and Prodanović, Slaven and Yan, Yueming",
year = "2017",
abstract = "Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and gliadins through intermolecular disulfide bond, confer dough viscoelasticity and wheat processing quality. Glutenins consist of high and low molecular weight glutenin subunits (HMW-GS, LMW-GS) encoded by Glu-1 and Glu-3 loci, respectively. The allelic variations at both loci have important effects on GMP content and breadmaking quality. In this study, GMP extraction and size-exclusion high performance liquid chromatography (SE-HPLC) separation were optimized, and then applied to investigate the effects of Glu-1 and Glu-3 allelic variations on GMP content using different wheat genotypes, chromosome substitution lines and near-isogenic lines (NILs). The results showed that the optimized GMP extraction and SE-HPLC protocol could obtain a reproducible separation and reliable quantitation of GMP content with small samples. The allelic variations at Glu-1 and Glu-3 were closely related to GMP content. Particularly, Glu-DId encoded 1Dx5+1Dy10 subunits, Glu-A3a and Glu-B3h encoded an abundant LMW-B subunit respectively had positive effects on GMP content and breadmaking quality. The introgress of HMW-GS and LMW-GS into bread wheat from related genomes could significantly increase GMP content, indicating that wheat related species has potential gene resources for breadmaking quality improvement. Our results demonstrated that SE-HPLC could serve as an effective tool for rapid separation and quantitation of GMPs and had potential application for gluten quality screening in the early generations during wheat quality improvement program.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc)",
pages = "691-677",
number = "2",
volume = "49",
doi = "10.2298/GENSR1702677W"
}

Wu, J., Lu, X., Yu, Z., Han, C., Li, X., Prodanović, S.,& Yan, Y.. (2017). Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc). in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 677-691.
https://doi.org/10.2298/GENSR1702677W

Wu J, Lu X, Yu Z, Han C, Li X, Prodanović S, Yan Y. Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc). in Genetika. 2017;49(2):677-691.
doi:10.2298/GENSR1702677W .

Wu, Jisu, Lu, Xiaobin, Yu, Zitong, Han, Caixia, Li, Xiaohui, Prodanović, Slaven, Yan, Yueming, "Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (gmp) content as revealed by size-exclusion high performance liquid chromatography (se-hplc)" in Genetika, 49, no. 2 (2017):677-691,
https://doi.org/10.2298/GENSR1702677W . .

TY  - JOUR
AU  - Dong, Liwei
AU  - Li, Ning
AU  - Lu, Xiaobing
AU  - Prodanović, Slaven
AU  - Xu, Yanhao
AU  - Zhang, Wenying
AU  - Yan, Yueming
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4191
AB  - Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines
EP  - 269
IS  - 1
SP  - 249
VL  - 48
DO  - 10.2298/GENSR1601249D
ER  - 

@article{
author = "Dong, Liwei and Li, Ning and Lu, Xiaobing and Prodanović, Slaven and Xu, Yanhao and Zhang, Wenying and Yan, Yueming",
year = "2016",
abstract = "Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+ 10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+ 12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+ 10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines",
pages = "269-249",
number = "1",
volume = "48",
doi = "10.2298/GENSR1601249D"
}

Dong, L., Li, N., Lu, X., Prodanović, S., Xu, Y., Zhang, W.,& Yan, Y.. (2016). Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 249-269.
https://doi.org/10.2298/GENSR1601249D

Dong L, Li N, Lu X, Prodanović S, Xu Y, Zhang W, Yan Y. Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines. in Genetika. 2016;48(1):249-269.
doi:10.2298/GENSR1601249D .

Dong, Liwei, Li, Ning, Lu, Xiaobing, Prodanović, Slaven, Xu, Yanhao, Zhang, Wenying, Yan, Yueming, "Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines" in Genetika, 48, no. 1 (2016):249-269,
https://doi.org/10.2298/GENSR1601249D . .

TY  - JOUR
AU  - Zhu, Gengrui
AU  - Wang, Shunli
AU  - Zhen, Shoumin
AU  - Shen, Xixi
AU  - Prodanović, Slaven
AU  - Yan, Yueming
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3839
AB  - Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima
EP  - 203
IS  - 1
SP  - 185
VL  - 47
DO  - 10.2298/GENSR1501185Z
ER  - 

@article{
author = "Zhu, Gengrui and Wang, Shunli and Zhen, Shoumin and Shen, Xixi and Prodanović, Slaven and Yan, Yueming",
year = "2015",
abstract = "Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima",
pages = "203-185",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501185Z"
}

Zhu, G., Wang, S., Zhen, S., Shen, X., Prodanović, S.,& Yan, Y.. (2015). Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 185-203.
https://doi.org/10.2298/GENSR1501185Z

Zhu G, Wang S, Zhen S, Shen X, Prodanović S, Yan Y. Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima. in Genetika. 2015;47(1):185-203.
doi:10.2298/GENSR1501185Z .

Zhu, Gengrui, Wang, Shunli, Zhen, Shoumin, Shen, Xixi, Prodanović, Slaven, Yan, Yueming, "Molecular characterization and phylogenetic analysis of unusual x-type hmw glutenin subunits from 1s(l) genome of aegilops longissima" in Genetika, 47, no. 1 (2015):185-203,
https://doi.org/10.2298/GENSR1501185Z . .

TY  - JOUR
AU  - Perović, Dragan
AU  - Zorić, Dragica
AU  - Milovanović, Milivoje
AU  - Prodanović, Slaven
AU  - Yan, Yueming
AU  - Janković, Snežana
AU  - Šurlan-Momirović, Gordana
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2077
AB  - The presence of two maternal chromosome sets in triploid barley endosperm allows the distinction of maternal and paternal hordein bands in an electrophoregram: the maternal bands are stronger due to the higher gene dose. In the F1 generation there are differences between reciprocal crosses and in the F2 generation all 16 classes that are theoretically possible for a pair of polymorphic loci can be distinguished. This full classification is rarely possible in genetic studies, and allows more accurate estimates of recombination rates. Two hordein gene clusters (Hor1 and Hor2, corresponding to hordein C and hordein B respectively) were analyzed in hybrids obtained by crossing two winter barley cultivars Partizan and HWV-247. Hordein separation was performed by acid-polyacrylamide gel electrophoresis at pH 3.2 (A-PAGE). A set of most informative bands of B and C hordeins was selected in each cross by two criteria: (1) presence or absence of bands in the parents and (2) signal strength to allow doses scoring. The average genetic distance between Hor1 and Hor2 loci was 11 cM. Distances in male and female maps were not significantly different, suggesting a similar recombination rate in male and female meiosis.
AB  - Prisustvo dva majčinska seta hromozoma u triploidnom endospermu zrna ječma omogućuje razlikovanje hordeinskih traka u elektroforegramu koje potiču od majke i oca: majčinske komponente su jače ispoljene usled dejstva efekta doze gena. U F1 generaciji vidljive su razlike kod recipročnog ukrštanja, a u F2 generaciji je moguće razlikovati svih 16 teoretski očekivanih klasa za par polimorfnih lokusa. Ova potpuna klasifikacija redak je fenomen u genetičkim studijama te zbog toga omogućuje precizniju procenu rekombinacija. Dve grupe hordeinskih gena, Hor1 i Hor2 koji kodiraju C I B hordeine, su analizirane kod hibrida nastalih ukrštanjem dve ozime sorte ječma Partizan i HWV-247. Separacija hordeina je izvršena primenom kisele poliakrilamidne gel elektriforeze pri pH 3.2 (A-PAGE). Set najinformativnijih hordeinskih trajka za B i C hordeine je odabran na osnovu sledećih kriterijuma: (1) prisustvo ili odsustvo trake kod roditelja i (2) jačina signala koja omogućava ocenu efekta doze gena. Prosečna genetička distance između Hor1 i Hor2 lokusa bila je 11 cM. Genetičke distance kod roditeljskih komponenti nisu se statistički značajno razlikovale što sugeriše sličnu distribuciju rekombinacija u obe mejoze.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.)
T1  - Efekti doze gena kod hordeina u triploidnom endospermu ječma (Hordeum vulgare L.)
EP  - 287
IS  - 3
SP  - 271
VL  - 41
DO  - 10.2298/GENSR0903271P
ER  - 

@article{
author = "Perović, Dragan and Zorić, Dragica and Milovanović, Milivoje and Prodanović, Slaven and Yan, Yueming and Janković, Snežana and Šurlan-Momirović, Gordana",
year = "2009",
abstract = "The presence of two maternal chromosome sets in triploid barley endosperm allows the distinction of maternal and paternal hordein bands in an electrophoregram: the maternal bands are stronger due to the higher gene dose. In the F1 generation there are differences between reciprocal crosses and in the F2 generation all 16 classes that are theoretically possible for a pair of polymorphic loci can be distinguished. This full classification is rarely possible in genetic studies, and allows more accurate estimates of recombination rates. Two hordein gene clusters (Hor1 and Hor2, corresponding to hordein C and hordein B respectively) were analyzed in hybrids obtained by crossing two winter barley cultivars Partizan and HWV-247. Hordein separation was performed by acid-polyacrylamide gel electrophoresis at pH 3.2 (A-PAGE). A set of most informative bands of B and C hordeins was selected in each cross by two criteria: (1) presence or absence of bands in the parents and (2) signal strength to allow doses scoring. The average genetic distance between Hor1 and Hor2 loci was 11 cM. Distances in male and female maps were not significantly different, suggesting a similar recombination rate in male and female meiosis., Prisustvo dva majčinska seta hromozoma u triploidnom endospermu zrna ječma omogućuje razlikovanje hordeinskih traka u elektroforegramu koje potiču od majke i oca: majčinske komponente su jače ispoljene usled dejstva efekta doze gena. U F1 generaciji vidljive su razlike kod recipročnog ukrštanja, a u F2 generaciji je moguće razlikovati svih 16 teoretski očekivanih klasa za par polimorfnih lokusa. Ova potpuna klasifikacija redak je fenomen u genetičkim studijama te zbog toga omogućuje precizniju procenu rekombinacija. Dve grupe hordeinskih gena, Hor1 i Hor2 koji kodiraju C I B hordeine, su analizirane kod hibrida nastalih ukrštanjem dve ozime sorte ječma Partizan i HWV-247. Separacija hordeina je izvršena primenom kisele poliakrilamidne gel elektriforeze pri pH 3.2 (A-PAGE). Set najinformativnijih hordeinskih trajka za B i C hordeine je odabran na osnovu sledećih kriterijuma: (1) prisustvo ili odsustvo trake kod roditelja i (2) jačina signala koja omogućava ocenu efekta doze gena. Prosečna genetička distance između Hor1 i Hor2 lokusa bila je 11 cM. Genetičke distance kod roditeljskih komponenti nisu se statistički značajno razlikovale što sugeriše sličnu distribuciju rekombinacija u obe mejoze.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.), Efekti doze gena kod hordeina u triploidnom endospermu ječma (Hordeum vulgare L.)",
pages = "287-271",
number = "3",
volume = "41",
doi = "10.2298/GENSR0903271P"
}

Perović, D., Zorić, D., Milovanović, M., Prodanović, S., Yan, Y., Janković, S.,& Šurlan-Momirović, G.. (2009). Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.). in Genetika
Društvo genetičara Srbije, Beograd., 41(3), 271-287.
https://doi.org/10.2298/GENSR0903271P

Perović D, Zorić D, Milovanović M, Prodanović S, Yan Y, Janković S, Šurlan-Momirović G. Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.). in Genetika. 2009;41(3):271-287.
doi:10.2298/GENSR0903271P .

Perović, Dragan, Zorić, Dragica, Milovanović, Milivoje, Prodanović, Slaven, Yan, Yueming, Janković, Snežana, Šurlan-Momirović, Gordana, "Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.)" in Genetika, 41, no. 3 (2009):271-287,
https://doi.org/10.2298/GENSR0903271P . .